Abstract：
PURPOSE:Virulence genes play important roles in pathogenesis of infections caused by S. aureus. The aim of this study was to determine the prevalence of PVL, eta and mecA genes in S. aureus isolated from patients in South-Western Nigeria. MATERIALS AND METHODS:In this study, a total of 116 S. aureus isolates from the clinical specimens submitted to laboratories in tertiary hospitals in the South Western Nigeria were used. Antibiotic susceptibility test was carried out to determine the susceptibility pattern of the isolates using multiple antibiotics disc. Minimum inhibitory concentration (MIC) was also carried out to determine the degree of resistant of the isolates to methicillin. PCR was used to screen for the presence of PVL, eta, and mecAgenes. RESULTS:mecA gene was detected in 48 (41.4%) of 116 strains of S. aureus. The MIC 50 and MIC 90 for mecA negative strains were 1 and 8 μg/ml, respectively while the MIC 50 and MIC 90 for mecA positive were >256 μg/ml. Twenty eight (24.1%) of 116 isolates were PVL gene positive with none of them mecA+. The prevalence of community acquired MRSA (CA-MRSA) was estimated to be 6.9% using molecular techniques. No localization of mecA gene and PVL gene on the genome of the entire S. aureus strains studied. Site of isolation of organism /specimen type was found to be associated with the prevalence of PVL+ and mecA+ S. aureus (P< 0.01). CONCLUSION:This study concludes that the PVL+ MRSA is rare and the prevalence of CA-MRSA is low in South-Western, Nigeria.
journal_name
Indian J Med Microbioljournal_title
Indian journal of medical microbiologyauthors
Terry Alli OA,Ogbolu DO,Mustapha JO,Akinbami R,Ajayi AOdoi
10.4103/0255-0857.96675subject
Has Abstractpub_date
2012-04-01 00:00:00pages
159-64issue
2eissn
0255-0857issn
1998-3646pii
IndianJMedMicrobiol_2012_30_2_159_96675journal_volume
30pub_type
杂志文章abstract：BACKGROUND:Culture filtrate proteins (CFPs) of Mycobacterium tuberculosis are potential vaccine candidates. OBJECTIVE:The aim was to study the influence of iron levels on CFPs and assess the immuno-protective potential of defined antigenic fractions from high (8 μg Fe/mL) and low iron (0.02 μg Fe / mL) cultures of M. ...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/0255-0857.99495
更新日期：2012-07-01 00:00:00
abstract：:Seroprevalence of Rubella was determined in 580 women including 80 women of medical community of district Amritsar, by ELISA test. The overall Rubella IgG seropositivity was 68.8% while in women of medical community it was 80%. Maximum number of women were seropositive (77.2%) in age group 26-35 years. Significantly h...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:Disseminated cases of histoplasmosis in acquired immune deficiency syndrome (AIDS) are rarely reported from India. Most of these cases report isolation of this fungus from the bone marrow, lymph node aspirate, spleenic aspirate, and biopsies. We report isolation of Histoplasma capsulatum from the blood of an AIDS pati...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/0255-0857.81792
更新日期：2011-04-01 00:00:00
abstract：:The aim of this study was to explore baseline data, laboratory and molecular analyses to determine if any could serve as potential prognostic marker(s) for treatment response to second line tuberculosis regimens. Of a total number of 50 multi-drug resistant tuberculosis (MDR-TB) patients starting second-line drug MDR-...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/0255-0857.118899
更新日期：2013-10-01 00:00:00
abstract：PURPOSE:The Western Blot test is considered a gold standard test for the confirmation of an ELISA and/or rapid assay screened reactive sample in the diagnosis of HIV infection, especially in the low risk population. In this study, an indigenously developed HIV W. Blot kit (J.Mitra & Co., New Delhi, India) was compared ...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：
更新日期：2002-10-01 00:00:00
abstract：Background:Raoultella is a Gram-negative bacteria, which commonly occur in the natural environment such as water, soil and on plants. In recent years, Raoultella spp. gained more interest. There is also an increasing number of publications describing mainly clinical cases involving these bacteria. Identification of Rao...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/ijmm.IJMM_17_99
更新日期：2018-04-01 00:00:00
abstract：:The detection of extended-spectrum beta-lactamases (ESBLs) in gram-negative bacteria that produce AmpC beta-lactamases is problematic. In the present study, the performance of modified double-disc synergy test (MDDST) that employs a combination of cefepime and piperacillin-tazobactam for the detection of Proteus mirab...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/0255-0857.38860
更新日期：2008-01-01 00:00:00
abstract：:An outbreak of food poisoning due to Salmonella enterica serotype Weltevreden ( S.weltevreden ) involving 34 students has been reported from a tertiary care hospital in Mangalore. The symptoms developed 8-10 hours, after consuming a non- vegetarian dish, probably fish, from an outside caterer. The identity of the orga...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/0255-0857.53211
更新日期：2009-07-01 00:00:00
abstract：:Ocular nocardiosis is an opportunistic infection and is believed to be a rare entity. We describe a rare case report of a patient with culture-positive Nocardia asteroides canaliculitis who presented with complaints of watering, purulent discharge and painful swelling of left lower eye lid. A purulent tenacious materi...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：
更新日期：2004-04-01 00:00:00
abstract：PURPOSE:Genotyping studies like spoligotyping are valuable tools in understanding the genetic diversity and epidemiology of Mycobacterium tuberculosis. Though there are reports of spoligotyping of M. tuberculosis isolates from pulmonary specimens from different parts of India, spoligotyping of extra pulmonary tuberculo...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/0255-0857.154871
更新日期：2015-04-01 00:00:00
abstract：PURPOSE:The present study was undertaken to detect metallo-beta-lactamase (MBL) in nosocomial isolates of Pseudomonas aeruginosa by four different phenotypic methods. METHODS:Ninety-one consecutive P. aeruginosa isolates were subjected to susceptibility testing by disc-diffusion assay and Vitek 2. Imipenem resistance ...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/0255-0857.39587
更新日期：2008-07-01 00:00:00
abstract：:We report the first case of prosthetic joint infection caused by Lysobacter thermophilus which was identified by 16S rRNA gene sequencing. Removal of prosthesis followed by antibiotic treatment resulted in good clinical outcome. This case illustrates the use of molecular diagnostics to detect uncommon organisms in sus...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/0255-0857.174124
更新日期：2016-01-01 00:00:00
abstract：:A male infant admitted with pyogenic meningitis with protein energy malnutrition developed fatal infection due to Salmonella enteritidis. The same organism was isolated from CSF and blood cultures. ...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：:A 23-year-old male patient who was a follow-up case of neurosurgery presented to our emergency department with a history of high-grade fever and clinical features of meningitis for 1 week. The cerebrospinal fluid (CSF) was sent to our laboratory for culture. The culture demonstrated growth of 1-2 mm in diameter light ...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/0255-0857.115653
更新日期：2013-07-01 00:00:00
abstract：:Legionella pneumophila is one of the important pathogen responsible for community -acquired pneumonia attributing for 1-5% of cases. Since early and accurate therapy reduces mortality, rapid and reliable diagnostic methods are needed. A total of 134 samples of blood, urine and respiratory tract fluids were collected. ...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/0255-0857.195353
更新日期：2016-10-01 00:00:00
abstract：PURPOSE:The purpose of this study was to evaluate the discriminatory power of two DNA-based technique and a protein-based technique for the typing of nosocomial isolates of Klebsiella pneumoniae. A second objective was to determine the antimicrobial susceptibility pattern and characterise the presence of genes encoding...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/ijmm.IJMM_16_308
更新日期：2017-07-01 00:00:00
abstract：:The surveillance in Chennai identified 134 children and 443 adults clinically suspected for leptospirosis. Of these, 35 (26.1%) children and 118 (26.6%) adults had laboratory confirmed diagnosis for leptospirosis. The paediatric leptospirosis exhibited a higher frequency of classic features of Weil's disease. The prev...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/0255-0857.180353
更新日期：2016-04-01 00:00:00
abstract：:Corynebacterium striatum is an emerging nosocomial pathogen associated with wound infections, pneumonia and meningitis. It is also a multidrug-resistant pathogen causing high morbidity. This is a report of an unusual case of wound infection in a patient with laryngeal carcinoma. Accurate diagnosis of the infection and...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/0255-0857.136589
更新日期：2014-07-01 00:00:00
abstract：:Varied clinical presentations of Penicillium marneffei, an opportunistic pathogen in HIV disease has been rarely described in literature. We report a patient with advanced AIDS who presented to us with prolonged fever and had features of an acute abdomen. On radiologic imaging he had features of intestinal obstruction...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/0255-0857.40538
更新日期：2008-04-01 00:00:00
abstract：Background:The implementation of hospital infection prevention and control (IPC) in south Asia is not well described. We aimed to assess IPC programmes in hospitals in this region and explore opportunities for improvement. Methods:Attendees from hospitals in the South Asian Association for Regional Cooperation (SAARC)...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/ijmm.IJMM_18_82
更新日期：2018-10-01 00:00:00
abstract：AIMS:This study was undertaken to determine the rate of detection of rotavirus causing diarrhoea among children and adults, identify the common genotypes circulating and determine clinical correlates. SETTINGS AND DESIGN:This is a cross-sectional study in a tertiary care centre. MATERIALS AND METHODS:Stool samples we...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/ijmm.IJMM_16_51
更新日期：2017-04-01 00:00:00
abstract：:An ocular cysticercosis case of a 42-year-old male, who presented with anterior uveitis is being reported. Microscopical examination of the cyst revealed presence of only one hooklet suggestive of T. solium cysticercus. Mitochondrial DNA analysis confirmed it to be T. solium cysticercus of Asian genotype. This is the ...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/0255-0857.167319
更新日期：2015-10-01 00:00:00
abstract：:Serum samples from 465 subjects aged between 1 and 25 years were tested for antibody against hepatitis A virus (HAV) [anti-HAV IgG and IgM] to determine the seroprevalence of HAV antibody and do a cost-benefit analysis for decision making about vaccination against HAV among the general population of Bangladesh. A high...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：PURPOSE:Opportunistic viral infections cause increased morbidity and mortality among human immunodeficiency virus (HIV) infected individuals, especially those who are not on antiretroviral treatment. Early diagnosis of these opportunistic viruses will be able to reduce the risk of disease progression with appropriate i...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/0255-0857.53202
更新日期：2009-07-01 00:00:00
abstract：PURPOSE:To determine the risk factors, microbiological features, clinical features and other epidemiological characteristics of Nocardia keratitis seen at a tertiary eye care centre in south India. METHODS:We evaluated 31 patients with Nocardia keratitis seen over two years, from September 1999 to September 2001. Corn...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:Fusarium is an emerging human opportunistic pathogen of growing importance, especially among immunosuppressed haematology patients due to an increased incidence of disseminated infections over the past two decades. This trend is expected only to continue due to the advances in medical and surgical technologies that wi...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章,评审
doi：10.4103/ijmm.IJMM_16_24
更新日期：2018-01-01 00:00:00
abstract：PURPOSE:Hepatitis B surface Antigen (HBsAg) is the hallmark in diagnosing hepatitis B virus (HBV) infection. In India many commercial assays are available for detection of HBsAg but very few can measure it quantitatively. The present study presents the comparative evaluation of two methods and their correlation with se...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/0255-0857.148400
更新日期：2015-01-01 00:00:00
abstract：BACKGROUND:Mycobacterial antigen MPB64 is a secretory protein specific for Mycobacterium tuberculosis complex. A lateral flow immunochromatographic assay (ICA) is a method used for the rapid differentiation of M. tuberculosis complex. AIM:We aimed to evaluate the performance of ICA in rapid differentiation of M. tuber...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/0255-0857.71817
更新日期：2010-10-01 00:00:00
abstract：BACKGROUND:Detecting plasmid-mediated AmpC (pAmpC) β-lactamase-producing organism is important for optimal infection control and providing accurate and effective treatment option for physicians. OBJECTIVES:The aim of this study was to investigate the prevalence of pAmpC β-lactamase and compare the results of boronic a...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/0255-0857.108723
更新日期：2013-01-01 00:00:00
abstract：Background and Objectives:Human papillomavirus (HPV) is the causative agent of cervical cancer, a major cause of cancer mortality in Indian women. The current study was undertaken to add information to the existing data on HPV type distribution in Indians, in an attempt to document HPV types for future vaccination prog...
journal_title：Indian journal of medical microbiology
pub_type： 杂志文章
doi：10.4103/ijmm.IJMM_19_451
更新日期：2019-10-01 00:00:00
