Spoligotype patterns of Mycobacterium tuberculosis isolated from extra pulmonary tuberculosis patients in Puducherry, India.

Abstract:

PURPOSE:Genotyping studies like spoligotyping are valuable tools in understanding the genetic diversity and epidemiology of Mycobacterium tuberculosis. Though there are reports of spoligotyping of M. tuberculosis isolates from pulmonary specimens from different parts of India, spoligotyping of extra pulmonary tuberculosis isolates are very few. Puducherry has not yet recorded spoligopatterns of M. tuberculosis from either pulmonary or extra pulmonary (EPTB) specimens. The aim of this study is to analyze the spoligotype patterns of EPTB strains circulating in Puducherry and neighboring districts of Tamil Nadu. MATERIALS AND METHODS:During June 2011 to December 2013, 570 EPTB specimens were processed by culturing on to Lowenstein Jensen (LJ) medium and automated Mycobacterium Growth Indicator Tube system (MGIT960). Identification of M. tuberculosis was carried out as per standard procedures, and MPT 64 antigen positivity in a commercial immunochromatography kit. Spoligotyping was carried out at National Institute of Research in Tuberculosis (ICMR), Chennai. RESULTS:M. tuberculosis was isolated from 67 single EPTB specimens (11.8%) like pus/cold abscess (34), TB spine (10), pleural fluid (10), urine (5), tissue bit (2), lymph nodes (2), ascitic fluid (2), synovial fluid (1) and endometrial curetting (1). Among 67 isolates with 41 spoligopatterns, EAI lineage with 28 isolates (41.8%) predominated followed by 18 orphans (26.9%), 10 Beijing (14.9%) and 8 U (11.9%). BOVIS1_BCG (ST482), T1-T2 (ST78) and H3 (ST50) were represented by one strain each (1.5%). C onclusions: Spoligotyping plays a significant role in the epidemiology of tuberculosis. Three spoligotypes, T1-T2 (ST78), EAI6 (ST292) and U (ST1429) are reported for the first time in India.

journal_name

Indian J Med Microbiol

authors

Kandhakumari G,Stephen S,Sivakumar S,Narayanan S

doi

10.4103/0255-0857.154871

subject

Has Abstract

pub_date

2015-04-01 00:00:00

pages

267-70

issue

2

eissn

0255-0857

issn

1998-3646

pii

IndianJMedMicrobiol_2015_33_2_267_154871

journal_volume

33

pub_type

杂志文章
  • Investigation of an outbreak of varicella in Chandigarh, North India, using a real-time polymerase chain reaction approach.

    abstract::Outbreaks of varicella are reported when susceptible population accumulates. This study reports a chickenpox outbreak in Burail in August 2014, wherein 20 laboratory-confirmed cases were identified by the detection of varicella zoster virus (VZV) DNA and VZV IgM antibodies. The viral load between vesicular swabs and s...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_16_420

    authors: Singh MP,Kaur R,Kumar A,Gupta M,Garg S,Ratho RK

    更新日期:2017-07-01 00:00:00

  • Serotyping of dengue viruses circulating during 2014-2015 in Assam, India.

    abstract::Dengue has become endemic in India with outbreaks caused by all the four serotypes occurring almost every year. Dengue cases have been increasing alarmingly in Assam also. This study aimed to identify the dengue serotypes circulating in Assam. Clinically suspected dengue fever cases were included in the study. Viral R...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_17_121

    authors: Rahman M,Sharma A,Patgiri S,Hussain E,Borah AK,Saikia L

    更新日期:2018-07-01 00:00:00

  • Superantigens - concepts, clinical disease and therapy.

    abstract::The term superantigen was introduced in microbiology and immunology only a decade ago. The unique feature of superantigen is that it bypasses the antigen processing mechanism and specifically binds to TCR v segment and forms a trimolecular complex along with major histocompatibility complex class II. Since its discove...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Hemalatha V,Srikanth P,Mallika M

    更新日期:2004-10-01 00:00:00

  • A pilot study of Chlamydia trachomatis pneumonia in infants.

    abstract::Chlamydia trachomatis is currently recognized as one of the most common sexually transmitted pathogen and a major cause of perinatally acquired infection. Vertical transmission of infection from mother to the infant may result in the development of conjunctivitis and pneumonia. The study was conducted with the aim of ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Chawla R,Bhalla P,Sachdev HP

    更新日期:2004-07-01 00:00:00

  • Cutaneous leishmaniasis: an emerging infection in a non-endemic area and a brief update.

    abstract::We report here the emergence of a new focus of cutaneous leishmaniasis (CL) due to Leishmania tropica (L. tropica) in the Ajmer city of Rajasthan, India, a previously non-endemic area. Between January-February 2006, 13 new indigenously acquired cases of CL were diagnosed among the patients attending the Skin and STD d...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.34774

    authors: Rastogi V,Nirwan PS

    更新日期:2007-07-01 00:00:00

  • Learning from an outbreak: ESBL- the essential points.

    abstract::Extended spectrum beta-lactamase (ESBL) producing strains of Klebsiella pneumoniae have emerged as important nosocomial pathogens. The present report describes an outbreak of ESBL positive K. pneumoniae in a neonatal intensive care unit of a tertiary care center in southern India. The clinical and microbiological sign...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Shanmuganathan C,Ananthakrishnan A,Jayakeerthi SR,Kanungo R,Kumar A,Bhattacharya S,Badrinath S

    更新日期:2004-10-01 00:00:00

  • Prosthetic joint infection due to Lysobacter thermophilus diagnosed by 16S rRNA gene sequencing.

    abstract::We report the first case of prosthetic joint infection caused by Lysobacter thermophilus which was identified by 16S rRNA gene sequencing. Removal of prosthesis followed by antibiotic treatment resulted in good clinical outcome. This case illustrates the use of molecular diagnostics to detect uncommon organisms in sus...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.174124

    authors: Dhawan B,Sebastian S,Malhotra R,Kapil A,Gautam D

    更新日期:2016-01-01 00:00:00

  • Assessment of trends of ofloxacin resistance in Mycobacterium tuberculosis.

    abstract:PURPOSE:Ofloxacin (OFX) is one of the potent fluoroquinolone (FQ) recommended to treat MDR-TB. Over a decade, the preexposure of this drug for the treatment of other bacterial infections has resulted in acquisition of FQ resistance among Mycobacterium tuberculosis strains. Considering this possibility, a study was unde...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.83913

    authors: Verma JS,Nair D,Rawat D,Manzoor N

    更新日期:2011-07-01 00:00:00

  • Staphylococcus aureus colonisation in HIV-infected patients: Incidence, risk factors and subsequent skin- and soft-tissue infections.

    abstract::We evaluated the incidence and risk factors of Staphylococcus aureus colonisation in 300 treatment-naïve HIV patients. Swabs from anterior nares and pharynx were cultured. Eighty-eight patients (29.3%) were colonised with S. aureus (47.7% nasal, 23.8% pharyngeal and 28.5% at both sites), which yielded 112 isolates. Me...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_20_5

    authors: Panigrahy A,Sinha S,Das BK,Kapil A,Vishnubhatla S,Dhawan B

    更新日期:2020-07-01 00:00:00

  • Acute abdomen: an unusual presentation of disseminated Penicillium marneffei infection.

    abstract::Varied clinical presentations of Penicillium marneffei, an opportunistic pathogen in HIV disease has been rarely described in literature. We report a patient with advanced AIDS who presented to us with prolonged fever and had features of an acute abdomen. On radiologic imaging he had features of intestinal obstruction...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.40538

    authors: George IA,Sudarsanam TD,Pulimood AB,Mathews MS

    更新日期:2008-04-01 00:00:00

  • Characterisation of antimicrobial resistance in Salmonellae during 2014-2015 from four centres across India: An ICMR antimicrobial resistance surveillance network report.

    abstract:PURPOSE:The main purpose of this study was to establish 'Antimicrobial Resistance Surveillance Network' in India and to monitor the antimicrobial susceptibility profile of clinical isolates to establish a national network across the country for monitoring antimicrobial resistance in Salmonella. MATERIALS AND METHODS:T...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章,多中心研究

    doi:10.4103/ijmm.IJMM_16_382

    authors: Dahiya S,Sharma P,Kumari B,Pandey S,Malik R,Manral N,Veeraraghavan B,Pragasam AK,Ray P,Gautam V,Sistla S,Parija SC,Walia K,Ohri V,Das BK,Sood S,Kapil A

    更新日期:2017-01-01 00:00:00

  • Colistin susceptibility testing of gram-negative bacilli: Better performance of vitek2 system than E-test compared to broth microdilution method as the gold standard test.

    abstract:Introduction:Unavailability of optimal susceptibility testing (ST) challenges the clinical use of colistin. Broth microdilution (BMD), which is the reference for colistin ST, is inconvenient for diagnostics. Vitek2 and E-test although technically easier, are no longer recommended. Materials and Methods:For the evaluat...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_19_480

    authors: Das S,Roy S,Roy S,Goelv G,Sinha S,Mathur P,Walia K,Bhattacharya S

    更新日期:2020-01-01 00:00:00

  • Molecular characterisation of antimicrobial resistance in Pseudomonas aeruginosa and Acinetobacter baumannii during 2014 and 2015 collected across India.

    abstract:BACKGROUND:Surveillance of antimicrobial resistance (AMR) is of great importance. Pseudomonas aeruginosa and Acinetobacter baumannii are important pathogens and emergence of resistance in these have increased the morbidity and mortality rates. This surveillance study was initiated by the Government of India - Indian Co...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章,多中心研究

    doi:10.4103/0255-0857.195376

    authors: Pragasam AK,Vijayakumar S,Bakthavatchalam YD,Kapil A,Das BK,Ray P,Gautam V,Sistla S,Parija SC,Walia K,Ohri VC,Anandan S,Veeraraghavan B

    更新日期:2016-10-01 00:00:00

  • Seroprevalence, risk factors and genotype distribution for Hepatitis C infection: A study from rural hospital in Maharashtra.

    abstract:BACKGROUND AND OBJECTIVES:Hepatitis C is global health problem affecting a significant portion of the world's population. Available data in Western Maharashtra on seroprevalence, risk factors and genotype distribution are very limited. OBJECTIVES:The present study was carried out to estimate the seroprevalence, factor...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_16_96

    authors: Patil SR,Datkhile KD,Ghorpade MV,Patil SS,Kakade SV

    更新日期:2017-10-01 00:00:00

  • Prevalence of torch infections and its associated poor outcome in high-risk pregnant women of Central India: Time to think for prevention strategies.

    abstract:Introduction:The TORCH infections during pregnancy are associated with adverse congenital abnormalities, poor foetal outcome and subsequent reproductive failures. The absence of baseline data on status of TORCH infections and associated foetal outcomes prompted us to conduct the current study in Central India. Materia...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_20_136

    authors: Manjunathachar HV,Singh KN,Chouksey V,Kumar R,Sharma RK,Barde PV

    更新日期:2020-07-01 00:00:00

  • A study of tubercular lymphadenitis: a comparison of various laboratory diagnostic modalities with a special reference to tubercular polymerase chain reaction.

    abstract:OBJECTIVE:The purpose of our study was to compare various laboratory diagnostic methods, namely histopathological examination, Ziehl-Neelsen (ZN) stain, AFB culture by conventional Lowenstein-Jensen (LJ) method and fluorescence-based mycobacterial growth indicator tube (MGIT) technique and polymerase chain reaction (PC...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.90173

    authors: Patwardhan SA,Bhargava P,Bhide VM,Kelkar DS

    更新日期:2011-10-01 00:00:00

  • Pneumococcusuria: From bench to bedside.

    abstract::The present study highlights six cases of pneumococcusuria during the time period of May 2008 to May 2010. All the patients had a co-existing predisposing factor with the isolation of Streptococcus pneumoniae in urine. Five of the six patients having signs and symptoms of urinary tract infections (UTI) were treated an...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.93056

    authors: Krishna S,Sanjeevan KV,Sudheer A,Dinesh KR,Kumar A,Karim S

    更新日期:2012-01-01 00:00:00

  • Elizabethkingia meningoseptica: an emerging pathogen causing meningitis in a hospitalized adult trauma patient.

    abstract::A 23-year-old male patient who was a follow-up case of neurosurgery presented to our emergency department with a history of high-grade fever and clinical features of meningitis for 1 week. The cerebrospinal fluid (CSF) was sent to our laboratory for culture. The culture demonstrated growth of 1-2 mm in diameter light ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.115653

    authors: Tak V,Mathur P,Varghese P,Misra MC

    更新日期:2013-07-01 00:00:00

  • Identification of opportunistic enteric parasites among immunocompetent patients with diarrhoea from Northern India and genetic characterisation of Cryptosporidium and Microsporidia.

    abstract:PURPOSE:Enteric parasitic infestation is a major public health problem in developing countries. Parasites such as Cryptosporidium spp., Cyclospora spp., Cystoisospora spp. and Microsporidia may cause severe diarrhoea among immunocompromised patients. There is scanty data on their frequency among immunocompetent patient...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.174114

    authors: Ghoshal U,Dey A,Ranjan P,Khanduja S,Agarwal V,Ghoshal UC

    更新日期:2016-01-01 00:00:00

  • Safe transportation of biomedical waste in a health care institution.

    abstract:INTRODUCTION:The chances of health care waste (Biomedical waste) coming in contact with the health care workers, patients, visitors, sanitary workers, waste handlers, public, rag pickers and animals during transportation are high. MATERIALS AND METHODS:The study was conducted over a period of seven months (April 2013-...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.158559

    authors: Kumar A,Duggal S,Gur R,Rongpharpi SR,Sagar S,Rani M,Dhayal D,Khanijo CM

    更新日期:2015-07-01 00:00:00

  • Nanotechnology: a new frontier in virus detection in clinical practice.

    abstract::Researchers are expanding the applications of nanotechnology in the field of medicine since mid-2000. These technologies include nanoarrays, protein arrays, nanopore technology, nanoparticles as a contrivance in immunoassays and nanosensors, among others. Nanobiotechnologies are clinically applicable and possess the p...

    journal_title:Indian journal of medical microbiology

    pub_type: 社论,评审

    doi:

    authors: Abraham AM,Kannangai R,Sridharan G

    更新日期:2008-10-01 00:00:00

  • Detection of bacterial growth in blood components using oxygen consumption as a surrogate marker in a tertiary oncology setup.

    abstract::Microbiological contamination of blood and blood products is a well-recognised transfusion risk. This study was performed in the blood bank of our oncology centre, with an objective to detect bacterial contamination in our blood products using oxygen consumption as a surrogate marker [Pall Enhanced Bacterial Detection...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.96695

    authors: Chavan PD,Bhat VG,Ojha S,Kelkar RS,Rajadhyaksha SB,Marathe AN

    更新日期:2012-04-01 00:00:00

  • 16S rRNA PCR for differentiation of pathogenic and non-pathogenic leptospira isolates.

    abstract:PURPOSE:To determine the risk factors, microbiological features, clinical features and other epidemiological characteristics of Nocardia keratitis seen at a tertiary eye care centre in south India. METHODS:We evaluated 31 patients with Nocardia keratitis seen over two years, from September 1999 to September 2001. Corn...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:

    authors: Shukla J

    更新日期:2003-01-01 00:00:00

  • Assessment of antibacterial activity of levonadifloxacin against contemporary gram-positive clinical isolates collected from various Indian hospitals using disk-diffusion assay.

    abstract:Objectives:Levonadifloxacin is a novel benzoquinolizine subclass of quinolone with broad-spectrum activities against problematic pathogens such as methicillin-resistant Staphylococcus aureus, quinolone-resistant S. aureus, vancomycin intermediate S. aureus, and vancomycin-resistant S. aureus. Levonadifloxacin and its o...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_20_307

    authors: Baliga S,Mamtora DK,Gupta V,Shanmugam P,Biswas S,Mukherjee DN,Shenoy S

    更新日期:2020-07-01 00:00:00

  • Effectiveness of hand hygiene promotional program based on the WHO multimodal hand hygiene improvement strategy, in terms of compliance and decontamination efficacy in an indian tertiary level neonatal surgical intensive care unit.

    abstract:Background:The WHO Multimodal Hand Hygiene Improvement Strategy (MHHIS) has been proposed to improve the Hand Hygiene (HH) compliance of the WHO recommendations on HH.Therefore, the current study was planned in our neonatal unit with the objective of evaluating the effectiveness of a Hand Hygiene Promotional Program (H...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_20_47

    authors: Thomas AM,Kaur S,Biswal M,N Rao KL,Vig S

    更新日期:2019-10-01 00:00:00

  • Review of a 7-year record of the bacteriological profile of airway secretions of children with cystic fibrosis in North India.

    abstract:Background:Cystic fibrosis (CF) is now a recognised entity in India, with prevalence rates between 1/10,000 and 1/50,000. However, no data were available with regard to the profile of respiratory pathogens in the Indian setting. Materials and Methods:The records of respiratory secretion bacterial cultures of children ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/ijmm.IJMM_18_424

    authors: Gautam V,Kaza P,Mathew JL,Kaur V,Sharma M,Ray P

    更新日期:2019-04-01 00:00:00

  • Gut microbiota and its mysteries.

    abstract::Gut microbiota are microorganisms that inhabit the gut; they coexist peacefully with the host, thereby contributing to the health and well-being of individuals. Bacteroidetes and Firmicutes largely dominate the gut microbial flora. The intestinal flora promotes intestinal mucosal integrity, provides essential nutrient...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.4103/ijmm.IJMM_19_373

    authors: Pushpanathan P,Mathew GS,Selvarajan S,Seshadri KG,Srikanth P

    更新日期:2019-04-01 00:00:00

  • ESBL - from petri dish to the patient.

    abstract::Treatment of extended spectrum beta-lactamase (ESBL) producing strains of Enterobacteriaceae has emerged as a major challenge in hospitalised as well as community based patients. Infections due to ESBL producers range from uncomplicated urinary tract infection to life threatening sepsis. Although several reviews have ...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.4103/0255-0857.19889

    authors: Bhattacharya S

    更新日期:2006-01-01 00:00:00

  • Rapid identification of non-sporing anaerobes using nuclear magnetic resonance spectroscopy and an identification strategy.

    abstract:PURPOSE:The non-sporing anaerobes cause a wide spectrum of infections. They are difficult to culture and their identification is tedious and time-consuming. Rapid identification of anaerobes is highly desirable. Towards this end, the potential of nuclear magnetic resonance (NMR) spectroscopy for providing a fingerprint...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.37334

    authors: Menon S,Bharadwaj R,Chowdhary AS,Kaundinya DV,Palande DA

    更新日期:2007-10-01 00:00:00

  • Vertebro-cerebral cryptococcosis mimicking tuberculosis: a diagnostic dilemma in countries with high burden of tuberculosis.

    abstract::We report a case of a 30-year-old immunocompetent man with disseminated cryptococcosis who was initially treated with antitubercular therapy due to clinical and radiological diagnosis of vertebro-cerebral tuberculosis. The diagnosis of Cryptococcus infection was made due to incidental isolation of this fungus from blo...

    journal_title:Indian journal of medical microbiology

    pub_type: 杂志文章

    doi:10.4103/0255-0857.96715

    authors: Gupta R,Kushwaha S,Behera S,Jaiswal A,Thakur R

    更新日期:2012-04-01 00:00:00