Abstract:
:Mycobacterial diseases continue to cause high morbidity and mortality. Isolation, identification and sensitivity testing form the backbone of laboratory investigations. M. tuberculosis isolation needs 6-8 weeks on conventional egg containing media. For rapid isolation various methods have been evaluated. We evaluated biphasic system (Middlebrook 7H11 agar slant+Middlebrook 9H broth) in comparison with Lowenstein-Jensen (LJ) medium. In smear positive cases biphasic system showed the recovery rate of 97.05% as against 79.41% on LJ on incubation for 21+/-4.44 and 28+/-3.76 days respectively. In smear negative and culture positive cases biphasic system and LJ showed isolation rates of 91.66% and 66.6% after 36+/-3.44 and 41+/- 4.09 days respectively. Biphasic system showed lower contamination rate (1.33%). Biphasic medium is superior to LJ medium in isolation of M. tuberculosis.
journal_name
Indian J Med Microbioljournal_title
Indian journal of medical microbiologyauthors
Ghatole M,Sable C,Kamale P,Kandle S,Jahagirdar V,Yemul Vdoi
10.4103/0255-0857.16050subject
Has Abstractpub_date
2005-04-01 00:00:00pages
111-3issue
2eissn
0255-0857issn
1998-3646journal_volume
23pub_type
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更新日期:2010-07-01 00:00:00
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更新日期:2015-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
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doi:10.4103/0255-0857.115628
更新日期:2013-07-01 00:00:00
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pub_type: 杂志文章
doi:10.4103/0255-0857.115230
更新日期:2013-04-01 00:00:00
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journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
doi:
更新日期:2004-10-01 00:00:00
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更新日期:2004-07-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Indian journal of medical microbiology
pub_type: 杂志文章
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更新日期:2001-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-07-01 00:00:00