Evaluation of biphasic culture system for mycobacterial isolation from the sputum of patients with pulmonary tuberculosis.

Abstract:

:Mycobacterial diseases continue to cause high morbidity and mortality. Isolation, identification and sensitivity testing form the backbone of laboratory investigations. M. tuberculosis isolation needs 6-8 weeks on conventional egg containing media. For rapid isolation various methods have been evaluated. We evaluated biphasic system (Middlebrook 7H11 agar slant+Middlebrook 9H broth) in comparison with Lowenstein-Jensen (LJ) medium. In smear positive cases biphasic system showed the recovery rate of 97.05% as against 79.41% on LJ on incubation for 21+/-4.44 and 28+/-3.76 days respectively. In smear negative and culture positive cases biphasic system and LJ showed isolation rates of 91.66% and 66.6% after 36+/-3.44 and 41+/- 4.09 days respectively. Biphasic system showed lower contamination rate (1.33%). Biphasic medium is superior to LJ medium in isolation of M. tuberculosis.

journal_name

Indian J Med Microbiol

authors

Ghatole M,Sable C,Kamale P,Kandle S,Jahagirdar V,Yemul V

doi

10.4103/0255-0857.16050

subject

Has Abstract

pub_date

2005-04-01 00:00:00

pages

111-3

issue

2

eissn

0255-0857

issn

1998-3646

journal_volume

23

pub_type

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