Abstract：
:The purpose of this study was to characterize regulatory T cells (T(reg)) in skin lesions and peripheral blood from patients with dermatomyositis (DM) and to determine the serum levels of regulatory cytokines in the disease. In skin biopsy specimens from patients with DM, immunohistochemistry was performed for CD4(+), CD25(+), forkhead/winged helix transcription factor (FoxP3)(+), transforming growth factor (TGF)-β(+) and interleukin (IL)-10(+) cells. Additionally, we defined the number of T(reg) subpopulations in peripheral blood by flow cytometry using monoclonal antibodies against CD4, CD25, FoxP3, CD45RO, CD95, CCR4 and CLA. The levels of TGF-β and IL-10 were also determined in serum samples from patients with DM by enzyme-linked immunosorbent assays. Controls included patients with cutaneous lupus erythematosus, psoriasis and atopic dermatitis (AD) as well as healthy donors. The frequency of FoxP3(+) cells was significantly reduced in skin lesions from patients with DM (p < 0.001) compared to psoriasis and AD. Moreover, the number of cells positive for TGF-β was lower in DM than in psoriasis and AD, while IL-10(+) cells were significantly reduced only compared to psoriasis. The number of CD4(+)CD25(++)FoxP3(+) T(reg) in the peripheral blood of patients with DM was significantly reduced compared to healthy controls (p < 0.05), whereas other cell populations showed no significant differences. Finally, TGF-β and IL-10 serum levels were significantly lower in patients with DM compared to healthy controls (p < 0.05). These data suggest that the depletion of T(reg) and their main effector cytokines in the skin and the serum of patients with DM may be an important factor in the pathogenesis of the disease.
journal_name
J Autoimmunjournal_title
Journal of autoimmunityauthors
Antiga E,Kretz CC,Klembt R,Massi D,Ruland V,Stumpf C,Baroni G,Hartmann M,Hartschuh W,Volpi W,Del Bianco E,Enk A,Fabbri P,Krammer PH,Caproni M,Kuhn Adoi
10.1016/j.jaut.2010.07.006subject
Has Abstractpub_date
2010-12-01 00:00:00pages
342-50issue
4eissn
0896-8411issn
1095-9157pii
S0896-8411(10)00081-8journal_volume
35pub_type
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journal_title：Journal of autoimmunity
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journal_title：Journal of autoimmunity
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pub_type： 杂志文章
doi：10.1016/s0896-8411(05)80048-4
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journal_title：Journal of autoimmunity
pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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journal_title：Journal of autoimmunity
pub_type： 杂志文章,评审
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journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1006/jaut.1996.0004
更新日期：1996-02-01 00:00:00
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journal_title：Journal of autoimmunity
pub_type： 杂志文章
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更新日期：1991-04-01 00:00:00
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journal_title：Journal of autoimmunity
pub_type： 杂志文章,评审
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更新日期：1992-04-01 00:00:00
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journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1006/jaut.1996.0020
更新日期：1996-04-01 00:00:00
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journal_title：Journal of autoimmunity
pub_type： 杂志文章,评审
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更新日期：2012-08-01 00:00:00
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journal_title：Journal of autoimmunity
pub_type： 杂志文章
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journal_title：Journal of autoimmunity
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journal_title：Journal of autoimmunity
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doi：10.1006/jaut.1994.1003
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journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1006/jaut.1996.0047
更新日期：1996-06-01 00:00:00
abstract：OBJECTIVE:Evaluation of systematic epidemiological data regarding clinical characteristics, sex distribution and autoantibody pattern in Caucasian children with autoimmune hepatitis (AIH). STUDY DESIGN:Data of 142 children presenting with AIH (97 girls and 45 boys) have been analysed for their clinical, serological, a...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1016/j.jaut.2004.11.009
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abstract：:T cell clones reactive to beta-cell antigens prepared from different species were established in order to identify putative pathogenic T cells in human IDDM. We were able to generate T cell clones from patients, but not from controls, reactive specifically to the insulin secretory enriched fraction (ISG) of a rat insu...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1006/jaut.1995.0017
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abstract：:Systemic lupus erythematosus (SLE) is an autoimmune disorder with unknown aetiology. The major hallmark of this disease is the presence of antibodies against nuclear components, including double-stranded (ds)DNA and histones. The disease affects different organs, particularly the skin, kidneys and the nervous system. ...
journal_title：Journal of autoimmunity
pub_type： 杂志文章,评审
doi：10.1016/s0896-8411(02)00110-5
更新日期：2003-03-01 00:00:00
abstract：:The features of autoantibodies (autoAb) to liver fumarylacetoacetate hydrolase (FAH) elicited in mice infected with mouse hepatitis virus (MHV) were studied by ELISA and western-blot competition assays. All sera tested contained Ab to cryptic FAH epitopes according with results from western-blot tests, whereas ELISA d...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1016/j.jaut.2004.05.006
更新日期：2004-09-01 00:00:00
abstract：:Beta(2)-glycoprotein I (beta(2)GPI) is known as a major autoantigen for antiphospholipid antibodies. Our recent data show that binding of beta(2)GPI to oxidized low-density lipoprotein (oxLDL) or to liposomes containing anionic phospholipid(s) may facilitate the presentation of beta(2)GPI's epitope by macrophages/dend...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1016/j.jaut.2007.07.003
更新日期：2007-09-01 00:00:00
abstract：OBJECTIVE:To compare the efficacy and safety of Disease-modifying antirheumatic drugs (DMARDs) and anti-TNF-α agents in patients with non-infectious non-anterior uveitis. METHODS:Single center retrospective study including adult patients with non-infectious intermediate, posterior or pan-uveitis. Outcomes were compare...
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pub_type： 杂志文章
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更新日期：2020-09-01 00:00:00
