当前位置： SCI文献检索 > JOURNAL OF AUTOIMMUNITY期刊下所有文献 > Immunoblotting for the detection of TSH receptor autoantibodies.

Immunoblotting for the detection of TSH receptor autoantibodies.

Abstract：

:Immunoblotting was optimized to detect autoantibodies to TSH receptors from human and porcine thyroid tissue and to determine their epitope specificity. Autoantibodies to putative TSH receptor proteins in thyroid particulate membranes were detected in approximately 35% of sera from patients with Graves' disease. However, despite modifications to increase immunoblotting sensitivity and specificity, only a minority (less than 15%) of Graves' disease sera contained autoantibodies that identified epitopes within TSH affinity-purified human or porcine receptor proteins. In these sera there was no correlation between the TSH receptor antibody titre, determined by radioreceptor assay, and receptor epitope reactivity. The sensitivity of immunoblotting was limited by reduced transfer of purified receptor from the gel. However, in addition, the inability to immunoblot the purified receptor with a majority of Graves' sera, under conditions designed to enhance receptor renaturation, appears to reflect a strict conformational requirement for immunoreactivity. Immunoblotting of purified receptors therefore has a limited application in detecting, and defining the epitope reactivity of, TSH receptor autoantibodies.

journal_name

J Autoimmun

journal_title

Journal of autoimmunity

authors

Leedman PJ,Harrison PJ,Harrison LC

doi

10.1016/0896-8411(91)90164-8

subject

Has Abstract

pub_date

1991-06-01 00:00:00

pages

529-42

issue

3

eissn

0896-8411

issn

1095-9157

pii

0896-8411(91)90164-8

journal_volume

4

pub_type

杂志文章

相关文献

JOURNAL OF AUTOIMMUNITY文献大全
  • Characterization of regulatory T cells in patients with dermatomyositis.

    abstract：:The purpose of this study was to characterize regulatory T cells (T(reg)) in skin lesions and peripheral blood from patients with dermatomyositis (DM) and to determine the serum levels of regulatory cytokines in the disease. In skin biopsy specimens from patients with DM, immunohistochemistry was performed for CD4(+),...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1016/j.jaut.2010.07.006

    authors： Antiga E,Kretz CC,Klembt R,Massi D,Ruland V,Stumpf C,Baroni G,Hartmann M,Hartschuh W,Volpi W,Del Bianco E,Enk A,Fabbri P,Krammer PH,Caproni M,Kuhn A

    更新日期：2010-12-01 00:00:00

  • Type 1 cytokines polarize thymocytes during T cell development in adult thymus organ cultures.

    abstract：:Peripheral T cells can be polarized towards type 1 or type 2 cytokine immune responses during TCR engagement. Because T cell selection by peptide plus self-MHC in the thymus requires TCR engagement, we hypothesized that type 1 cytokines may polarize developing T cells. We cultured thymi from BBDR rats in adult thymus ...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1016/s0896-8411(02)00091-4

    authors： Whalen BJ,Marounek J,Mordes JP,Rossini AA,Greiner DL

    更新日期：2003-02-01 00:00:00

  • Therapeutic effect of CpG-enriched plasmid administration on the tight-skin mouse model of scleroderma.

    abstract：:Immunostimulatory CpG motifs can preferentially induce Th1 immune responses and have been applied to treat Th2-dominant disease. In this study, we investigated whether a plasmid with the addition of 20 copies of an immunostimulatory CpG motif (pB-CpG20) might prevent the development of scleroderma-like syndrome in tig...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1016/j.jaut.2005.01.014

    authors： Shen Y,Ichino M,Nakazawa M,Ikejima T,Kojima Y,Okuda K,Minami M

    更新日期：2005-05-01 00:00:00

  • Emergence of long-lived autoreactive plasma cells in the spleen of primary warm auto-immune hemolytic anemia patients treated with rituximab.

    abstract：:Primary warm autoimmune hemolytic anemia (wAIHA) is a rare autoimmune disease in which red blood cells are eliminated by IgG autoantibodies. We analyzed the antibody-secreting cells in the spleen and the peripheral blood of wAIHA patients in various contexts of treatment. Plasmablasts were observed in peripheral blood...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1016/j.jaut.2015.05.006

    authors： Mahévas M,Michel M,Vingert B,Moroch J,Boutboul D,Audia S,Cagnard N,Ripa J,Menard C,Tarte K,Mégret J,Le Gallou S,Patin P,Thai L,Galicier L,Bonnotte B,Godeau B,Noizat-Pirenne F,Weill JC,Reynaud CA

    更新日期：2015-08-01 00:00:00

  • Association of SLC11A1 promoter polymorphisms with the incidence of autoimmune and inflammatory diseases: a meta-analysis.

    abstract：:Solute carrier family 11 member a1 (SLC11A1) exerts pleiotropic effects on macrophage function. Expression of SLC11A1 is regulated by a (GT)(n) microsatellite promoter repeat polymorphism of which nine alleles have been described. Enhanced activation of macrophages, associated with increased expression from allele 3, ...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章,meta分析

    doi：10.1016/j.jaut.2008.02.002

    authors： O'Brien BA,Archer NS,Simpson AM,Torpy FR,Nassif NT

    更新日期：2008-08-01 00:00:00

  • Risk of progression to diabetes of low titer ICA-positive first-degree relatives of type I diabetics in southern Germany.

    abstract：:In a prospective study to evaluate the prevalence and predictive potential of circulating cytoplasmatic islet cell antibodies (ICA) and competitive insulin autoantibodies (CIAA), we screened 406 non-diabetic first-degree relatives of patients with Type I diabetes mellitus (n = 154 for CIAA). The prevalence of ICA was ...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1016/s0896-8411(05)80029-0

    authors： Ziegler AG,Baumgartl HJ,Standl E,Mehnert H

    更新日期：1990-10-01 00:00:00

  • Outcome of pregnancy and neonatal complications with anti-tumor necrosis factor-α use in females with immune mediated diseases; a systematic review and meta-analysis.

    abstract：BACKGROUND:Immune mediated diseases such as rheumatoid arthritis (RA), ankylosing spondylitis (AS), and inflammatory bowel disease (IBD) commonly affect young and adolescent females. Anti-tumor necrosis factor (TNF)-α agents are increasingly used to treat these conditions, but their safety during pregnancy remains uncl...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章,meta分析,评审

    doi：10.1016/j.jaut.2016.11.004

    authors： Komaki F,Komaki Y,Micic D,Ido A,Sakuraba A

    更新日期：2017-01-01 00:00:00

  • The deadly coronaviruses: The 2003 SARS pandemic and the 2020 novel coronavirus epidemic in China.

    abstract：:The 2019-nCoV is officially called SARS-CoV-2 and the disease is named COVID-19. This viral epidemic in China has led to the deaths of over 1800 people, mostly elderly or those with an underlying chronic disease or immunosuppressed state. This is the third serious Coronavirus outbreak in less than 20 years, following ...

    journal_title：Journal of autoimmunity

    pub_type： 历史文章,杂志文章,评审

    doi：10.1016/j.jaut.2020.102434

    authors： Yang Y,Peng F,Wang R,Yange M,Guan K,Jiang T,Xu G,Sun J,Chang C

    更新日期：2020-05-01 00:00:00

  • Regulatory dendritic cells in autoimmunity: A comprehensive review.

    abstract：:Dendritic cells (DCs) are professional antigen-presenting cells (APC) with significant phenotypic heterogeneity and functional plasticity. DCs play crucial roles in initiating effective adaptive immune responses for elimination of invading pathogens and also in inducing immune tolerance toward harmless components to m...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章,评审

    doi：10.1016/j.jaut.2015.07.011

    authors： Liu J,Cao X

    更新日期：2015-09-01 00:00:00

  • Dysregulation of innate and adaptive serum mediators precedes systemic lupus erythematosus classification and improves prognostic accuracy of autoantibodies.

    abstract：:Systemic lupus erythematosus (SLE) is a complex autoimmune disease with a poorly understood preclinical stage of immune dysregulation and symptom accrual. Accumulation of antinuclear autoantibody (ANA) specificities is a hallmark of impending clinical disease. Yet, many ANA-positive individuals remain healthy, suggest...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1016/j.jaut.2016.06.001

    authors： Lu R,Munroe ME,Guthridge JM,Bean KM,Fife DA,Chen H,Slight-Webb SR,Keith MP,Harley JB,James JA

    更新日期：2016-11-01 00:00:00

  • The top ten clues to understand the origin of chronic lymphocytic leukemia (CLL).

    abstract：:The fundamental task of the immune system is to protect the individual from infectious organisms without serious injury to self. The essence of acquired immunity is molecular self/non self discrimination. Chronic lymphocytic leukemia is characterized by a global failure of immune system that begins with the failure of...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章,评审

    doi：10.1016/j.jaut.2014.10.005

    authors： García-Muñoz R,Feliu J,Llorente L

    更新日期：2015-01-01 00:00:00

  • Acquisition of autoimmunity genes by New Zealand mice is associated with natural resistance to infection by mycobacteria.

    abstract：:New Zealand (NZ) mouse strains comprise both autoimmune and non-autoimmune animals: NZ black (NZB) mice and the F1 hybrid (NZB/W) of NZB and NZ white (NZW) mice show spontaneous autoimmune disease by 6 months of age and die before the first year of age from renal disease, while NZW mice do not show autoimmune disorder...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1016/0896-8411(92)90160-r

    authors： Esaguy N,Macedo PM,Castro AP,Aguas AP

    更新日期：1992-10-01 00:00:00

  • Dual-Targeted Autoimmune Sword in Fatal Epilepsy: Patient's glutamate receptor AMPA GluR3B peptide autoimmune antibodies bind, induce Reactive Oxygen Species (ROS) in, and kill both human neural cells and T cells.

    abstract：:Nodding Syndrome (NS) is a fatal pediatric epilepsy of unknown etiology, accompanied by multiple neurological impairments, and associated with Onchocerca volvulus (Ov), malnutrition, war-induced trauma, and other insults. NS patients have neuroinflammation, and ~50% have cross-reactive Ov/Leiomodin-1 neurotoxic autoim...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1016/j.jaut.2020.102462

    authors： Levite M,Zelig D,Friedman A,Ilouz N,Eilam R,Bromberg Z,Ramadhan Lasu AA,Arbel-Alon S,Edvardson S,Tarshish M,Riek LP,Lako RL,Reubinoff B,Lebendiker M,Yaish D,Stavsky A,Galun E

    更新日期：2020-08-01 00:00:00

  • Heritability versus the role of the environment in autoimmunity.

    abstract：:The higher concordant occurrence of autoimmune diseases in monozygotic twins compared to dizygotic or sibling pairs supports the role for genetic susceptibility. For most conditions, however, concordance rates are considerably below 100% and lead to the estimate of the weight of genetics coined "heritability". In the ...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章,评审

    doi：10.1016/j.jaut.2012.07.011

    authors： Selmi C,Lu Q,Humble MC

    更新日期：2012-12-01 00:00:00

  • 'Anergic' T cells modulate the T-cell activating capacity of antigen-presenting cells.

    abstract：:Nowadays there is compelling evidence for immunoregulation by T cells. Recently, we showed that so-called 'anergic' T cells are not functionally inert but can act as regulatory cells by actively suppressing other T cell responses. We now show that 'anergic' T cells mediate this suppressive effect via modulation of the...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1006/jaut.2000.0372

    authors： Taams LS,Boot EP,van Eden W,Wauben MH

    更新日期：2000-06-01 00:00:00

  • T-cell clone anti-clone interactions. Effects on suppressor and helper activities.

    abstract：:An experimental model of two interacting clones of T cells is described, which may be used for defining and exploring the T-cell immunoregulatory network. Mx9/9 is a CD4 clone bearing an antigen receptor recognized by the Mx9 anti-V beta 8 monoclonal antibody (MoAb). Anti-V beta 8 MoAbs activate and induce cell prolif...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1016/0896-8411(89)90112-1

    authors： Naor D,Essery G,Kahan M,Feldmann M

    更新日期：1989-06-01 00:00:00

  • Anti-CD4 treatment inhibits autoimmunity in scurfy mice through the attenuation of co-stimulatory signals.

    abstract：:A major concept in autoimmunity is that disruption of Foxp3(+) regulatory T cells (Tregs) predisposes to breach of tolerance. This is exemplified by the Foxp3-linked disorder termed IPEX (immunodysregulation, polyendocrinopathy, enteropathy, X-linked) which affects newborn children. There has been considerable clinica...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1016/j.jaut.2013.08.010

    authors： Mayer CT,Tian L,Hesse C,Kühl AA,Swallow M,Kruse F,Thiele M,Gershwin ME,Liston A,Sparwasser T

    更新日期：2014-05-01 00:00:00

  • Monocytes contribute to DNA sensing through the TBK1 signaling pathway in type 1 diabetes patients.

    abstract：BACKGROUND:The aberrant recognition of self-nucleic acids by the innate immune system contributes to the pathology of several autoimmune diseases. Although microbial DNA and, in certain instances, self-DNA that is released from damaged cells are primarily recognized by Toll-like receptor 9 (TLR9), recent evidence sugge...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1016/j.jaut.2019.06.005

    authors： Zentsova I,Parackova Z,Kayserova J,Palova-Jelinkova L,Vrabcova P,Volfova N,Sumnik Z,Pruhova S,Petruzelkova L,Sediva A

    更新日期：2019-12-01 00:00:00

  • Lack of requirement for CD8+ cells in recovery from and resistance to experimental autoimmune encephalomyelitis.

    abstract：:Experimental autoimmune encephalomyelitis (EAE) is a model of T-cell mediated autoimmune disease. Active disease is mediated by myelin basic protein specific CD4+ T-cells, whose adoptive transfer can also induce passive disease. In the Lewis rat EAE is a transient disease inducing lasting resistance to rechallenge. Th...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1006/jaut.1995.0031

    authors： Lohse AW,Schwerdt A,Herkel J,Spahn T,Meyer zum Büschenfelde KH

    更新日期：1995-06-01 00:00:00

  • Comparison of conventional immunosuppressive drugs versus anti-TNF-α agents in non-infectious non-anterior uveitis.

    abstract：OBJECTIVE:To compare the efficacy and safety of Disease-modifying antirheumatic drugs (DMARDs) and anti-TNF-α agents in patients with non-infectious non-anterior uveitis. METHODS:Single center retrospective study including adult patients with non-infectious intermediate, posterior or pan-uveitis. Outcomes were compare...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1016/j.jaut.2020.102481

    authors： Leclercq M,Langlois V,Girszyn N,Le Besnerais M,Benhamou Y,Levesque H,Muraine M,Gueudry J

    更新日期：2020-09-01 00:00:00

  • IA-2 antibody epitopes and isotypes during the prediabetic process in siblings of children with type 1 diabetes.

    abstract：:To characterize the humoral immune response to the protein tyrosine phosphatase (PTP)-like autoantigen (IA-2) in preclinical type 1 diabetes (T1D), and to assess the utility of epitope and isotype-specific IA-2 antibody responses as surrogate markers for disease development, we analyzed these antibodies in 34 initiall...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1016/j.jaut.2004.09.005

    authors： Hoppu S,Härkönen T,Ronkainen MS,Akerblom HK,Knip M,Childhood Diabetes in Finland Study Group.

    更新日期：2004-12-01 00:00:00

  • Hemodynamic improvement and removal of autoantibodies against beta1-adrenergic receptor by immunoadsorption therapy in dilated cardiomyopathy.

    abstract：:The removal of beta(1)-adrenergic receptor (beta(1)AR) autoantibodies by immunoadsorption (IA) has been proposed as a potential mechanism for the improvement of the left ventricular function in dilated cardiomyopathy (DCM). In the present study, the possible association between removal of the autoantibodies against th...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1016/s0896-8411(03)00042-8

    authors： Mobini R,Staudt A,Felix SB,Baumann G,Wallukat G,Deinum J,Svensson H,Hjalmarson A,Fu M

    更新日期：2003-06-01 00:00:00

  • The paternally inherited insulin gene B allele (1,428 FokI site) confers protection from insulin-dependent diabetes in families.

    abstract：:Several polymorphisms of the insulin gene and its flanking regions (INS region) are in linkage disequilibrium and confer susceptibility to insulin-dependent diabetes (IDDM). We have analysed INS AA and AB-BB genotypes at the 1,428 FokI site (3' of the insulin gene) in 217 patients with IDDM, 402 non-diabetic first deg...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1006/jaut.1994.1053

    authors： Pugliese A,Awdeh ZL,Alper CA,Jackson RA,Eisenbarth GS

    更新日期：1994-10-01 00:00:00

  • Juvenile autoimmune hepatitis: A comprehensive review.

    abstract：:Autoimmune hepatitis (AIH) is a rare, chronic disease that affects both adults and children, including infants. The disease is probably triggered by environmental factors in genetically predisposed individuals. The clinical presentation ranges from asymptomatic patients or patients with non-specific symptoms, such as ...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章,评审

    doi：10.1016/j.jaut.2018.10.007

    authors： Sokollik C,McLin VA,Vergani D,Terziroli Beretta-Piccoli B,Mieli-Vergani G

    更新日期：2018-12-01 00:00:00

  • Metagenomic profiling of the pro-inflammatory gut microbiota in ankylosing spondylitis.

    abstract：OBJECTIVE:Gut dysbiosis has been reported implicated in ankylosing spondylitis (AS), a common chronic inflammatory disease mainly affects sacroiliac joints and spine. Utilizing deep sequencing on the feces of untreated AS patients, our study aimed at providing an in-depth understanding of AS gut microbiota. METHODS:We...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1016/j.jaut.2019.102360

    authors： Zhou C,Zhao H,Xiao XY,Chen BD,Guo RJ,Wang Q,Chen H,Zhao LD,Zhang CC,Jiao YH,Ju YM,Yang HX,Fei YY,Wang L,Shen M,Li H,Wang XH,Lu X,Yang B,Liu JJ,Li J,Peng LY,Zheng WJ,Zhang CY,Zhou JX,Wu QJ,Yang YJ,Su

    更新日期：2020-02-01 00:00:00

  • TNF-like weak inducer of apoptosis promotes blood brain barrier disruption and increases neuronal cell death in MRL/lpr mice.

    abstract：:Neuropsychiatric disease is one of the most common manifestations of human systemic lupus erythematosus, but the mechanisms remain poorly understood. In human brain microvascular endothelial cells in vitro, TNF-like weak inducer of apoptosis (TWEAK) decreases tight junction ZO-1 expression and increases the permeabili...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1016/j.jaut.2015.03.005

    authors： Wen J,Doerner J,Weidenheim K,Xia Y,Stock A,Michaelson JS,Baruch K,Deczkowska A,Gulinello M,Schwartz M,Burkly LC,Putterman C

    更新日期：2015-06-01 00:00:00

  • Adoptive transfer of IL-10-secreting CD4+CD49b+ regulatory T cells suppresses ongoing arthritis.

    abstract：:We have previously demonstrated, in the collagen-induced arthritis model (CIA), that repetitive injections of immature bone-marrow-derived dendritic cells (iDCs) induce the expansion of a population of CD4CD49b-expressing cells, and that their adoptive transfer results in protection against CIA in a prophylactic setti...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1016/j.jaut.2009.10.003

    authors： Charbonnier LM,Han WG,Quentin J,Huizinga TW,Zwerina J,Toes RE,Jorgensen C,Louis-Plence P

    更新日期：2010-06-01 00:00:00

  • Regulatory T cell defect in APECED patients is associated with loss of naive FOXP3(+) precursors and impaired activated population.

    abstract：:The pathogenetic mechanisms of organ-specific autoimmune diseases remain obscured by the complexity of the genetic and environmental factors participating in the breakdown of tolerance. A unique opportunity to study the pathogenesis of human autoimmunity is provided by autoimmune polyendocrinopathy-candidiasis-ectoder...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1016/j.jaut.2010.07.008

    authors： Laakso SM,Laurinolli TT,Rossi LH,Lehtoviita A,Sairanen H,Perheentupa J,Kekäläinen E,Arstila TP

    更新日期：2010-12-01 00:00:00

  • Role of the first external domain of I-A beta chain in immune responses and diabetes in non-obese diabetic (NOD) mice.

    abstract：:Diabetes in the non-obese diabetic (NOD) mouse is a multigenic autoimmune disease and is possibly controlled by three recessive loci, including one that is linked to the major histocompatibility complex (MHC). The first external domain of the Class II MHC I-A beta chain in these mice is unique and has been suggested a...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1016/s0896-8411(05)80017-4

    authors： Singh B,Dillon T,Fraga E,Lauzon J

    更新日期：1990-10-01 00:00:00

  • Autoimmunity to the glutamate receptor in mice--a model for Rasmussen's encephalitis?

    abstract：:We investigated the in vivo pathogenic potential of murine autoimmunity to peptides of the glutamate/AMPA receptor subunit 3 (GluR3). Antibodies to GluR3 are found in human epilepsy, Rasmussen's encephalitis (RE). In our accompanying paper in this issue we found that murine antibodies to the GluR3B peptide (amino acid...

    journal_title：Journal of autoimmunity

    pub_type： 杂志文章

    doi：10.1006/jaut.1999.0297

    authors： Levite M,Hermelin A

    更新日期：1999-08-01 00:00:00