Abstract：
:In this work, the interaction between nano-TiO(2) and trypsin was investigated, and the mechanisms of the interaction were explored by the methods of UV-vis detection, circular dichroism (CD), and fluorescence. The results clearly demonstrated that nano-TiO(2) had an inhibitory effect on the enzyme activity. The activity was decreased to 64% of the untreated trypsin in the presence of 300 μg/ml nano-TiO(2). UV spectrometry proved that nano-TiO(2) had a strong physical absorption effect on trypsin, and the CD spectra revealed that the secondary structure of trypsin was partly destroyed while bound together with nano-TiO(2). The ratio of α-helix increased from 7.9% to 12.8% in the presence of 100 μg/ml TiO(2) while the ratio of β-sheet decreased from 48.7% to 36.4%. Furthermore, the fluorescence spectrometry indicated that nano-TiO(2) could quench the intrinsic fluorescence of trypsin through static quenching. Meanwhile, the binding constant was calculated to be 1, and the process of binding of trypsin on nano-TiO(2) was a spontaneous molecular interaction procedure in which electrostatic interaction plays a major role. Our study was to provide a useful approach for evaluating the health risk of nanomaterials on level of proteins.
journal_name
Biol Trace Elem Resjournal_title
Biological trace element researchauthors
Wang WR,Zhu RR,Xiao R,Liu H,Wang SLdoi
10.1007/s12011-010-8823-xsubject
Has Abstractpub_date
2011-09-01 00:00:00pages
435-46issue
3eissn
0163-4984issn
1559-0720journal_volume
142pub_type
杂志文章abstract：:Three kinds of plant leaves, Chinese white aspen, arborvitae, and pine needles, have been sampled from the Yanshan Oil refinery complex, the Capital Iron and Steel Factory, and Badachu, a control area in Beijing, as biomonitors for air-pollution studies. Each sample was divided into two parts, washed, and unwashed. Th...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02784212
更新日期：1999-01-01 00:00:00
abstract：:Sugar-borates (SBs) are mono- or di-sugar-borate esters (SBEs) comprised of one or two monosaccharide molecules linked to a boron (B) atom. SBEs occur naturally in commonly consumed herbs, vegetables, fruits, seeds, and nuts and, other than greatly varying levels of B found in local drinking water, are the primary nat...
journal_title：Biological trace element research
pub_type： 杂志文章,评审
doi：10.1007/s12011-018-1550-4
更新日期：2019-03-01 00:00:00
abstract：:In this study, eight kinds of nickel (Ni) compounds were orally administered to Wistar male rats and the distribution of each compound was investigated 24 h after the administration. The Ni compounds used in this experiment were nickel metal [Ni-M], nickel oxide (green) [NiO(G)], nickel oxide (black) [NiO(B)], nickel ...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02789001
更新日期：1995-07-01 00:00:00
abstract：:The association between serum copper and zinc concentrations and age, sex, and other risk factors of cardiovascular disease in randomly selected adult volunteers aged 19-59 were investigated. There was a positive relationship between copper and age in both sexes, but zinc was negatively correlated with age in males on...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02950795
更新日期：1994-03-01 00:00:00
abstract：:The distribution, potential ecological hazards, and homologous relativity of heavy metals in the water-level fluctuating zone (WLFZ) of the Yangtze River within the Wanzhou Section (YRWS) were investigated. The heavy metal content (Cd, Cu, Cr, and Ni) of the surface soil was analysed via atomic absorption spectroscopy...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-011-9179-6
更新日期：2012-02-01 00:00:00
abstract：:Recently, several attempts have been made to use the phytopharmaceuticals from plant extracts as reducing, capping and stabilizing agents for the biomimetic synthesis of various metal nanoparticles conjugated to the phytopharmaceuticals. These biogenic metal nanoparticles are non-toxic and can be used as contrast agen...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-018-1287-0
更新日期：2018-11-01 00:00:00
abstract：:Various studies indicate the role of manganese (Mn) in the virulence of pathogens. Salmonella is an intracellular pathogen which is able to multiply within the macrophages. The present study was therefore, designed to assess the effect of Mn supplementation on Salmonella-macrophage interactions particularly in referen...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-009-8406-x
更新日期：2010-01-01 00:00:00
abstract：:An "anti-oxidant cocktail" consisting of betacarotene, vitamins B6, C, E, zinc, and selenium or corresponding placebos were given for one y as daily dietary supplements to 45 elderly residents of a nursing home. Initially, the serum TBA reactant levels were higher (2.7 +/- 0.7 mumol/L) than those of an ad hoc control ...
journal_title：Biological trace element research
pub_type： 临床试验,杂志文章
doi：10.1007/BF02795458
更新日期：1988-09-01 00:00:00
abstract：:Aflatoxin B1 (AFB1) is a mycotoxin that causes cytotoxicity through oxidative damage to its target organs. The liver is the first target of AFB1 damage. The aim of this study was to evaluate the protective effect of selenium on AFB1-induced hepatic mitochondrial damage in ducklings using molecular biological and histo...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-014-0189-z
更新日期：2015-02-01 00:00:00
abstract：:Sea cucumbers are a potential source of natural organic vanadium that may improve insulin resistance. In this work, vanadium was accumulated rapidly in blood, body wall, and intestine by sea cucumber Apostichopus japonicus. Furthermore, water-soluble vanadium-containing proteins, the main form of the organic vanadium,...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-015-0517-y
更新日期：2016-05-01 00:00:00
abstract：:Soaking of a rat diet, high in both plant phytate and phytase, progressively degraded the phytate content with time of soaking. This dephytinization in turn enhanced the digestion of feed organic matter in the animals, and it significantly improved the absorption and retention of minerals and trace elements as observe...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02783810
更新日期：1993-10-01 00:00:00
abstract：:Cadmium (Cd) pollution and acid rain are the main environmental issues, and they often occur in the same agricultural region. Nevertheless, up to now, little information on the combined pollution of Cd(2+) and acid rain action on crops were presented. Here, we investigated the combined effect of Cd(2+) and acid rain o...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-011-9053-6
更新日期：2011-12-01 00:00:00
abstract：:This study was designed to measure the effect of iron supplementation on antioxidant status in iron-deficient anemia, including the time for hemoglobin normalization and at the time of filling of iron body stores. The extent of plasma lipid peroxidation was evaluated by measuring the levels of malondialdehyde and glut...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1385/BTER:96:1-3:117
更新日期：2003-01-01 00:00:00
abstract：:The seeds of Nelumbo nucifera (Lotus) have been used in the traditional system of medicine for various ailments including diabetes. The present study was aimed at analyzing the levels of biologically important trace elements in the lotus seeds by atomic absorption spectroscopy and evaluating the hypoglycemic propertie...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-010-8614-4
更新日期：2010-12-01 00:00:00
abstract：:Neurodegeneration disorders are complex and multifaceted, leading to many chronic diseases, like Parkinson's disease (PD). Electrolytes imbalance has a significant role in the pathophysiology of neurological disorders, which might serve as a bio-indicator of these problems. In the present study, the disturbances of th...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-020-02399-y
更新日期：2020-09-22 00:00:00
abstract：:Lead is a biohazardous metal that is commonly involved in human illness including renal injury. Although it is a non-redox reactive metal, lead-induced renal injury is largely based on oxidative stress. The current work aimed at exploring the possible protective effect of γ-glutamyl cysteine (γGC) against lead-induced...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-016-0624-4
更新日期：2016-09-01 00:00:00
abstract：:The degree of chromosome damage induced by three compounds of zinc (zinc chloride, zinc sulfate, zinc acetate) was compared in human leucocytes in vitro. Three concentrations of each salt, 3.0 x 10(-5)M, 3.0 x 10(-4)M, and 1.5 x 10(-3)M, were added to leukocyte cultures. The cells were harvested after 48 and 72 h and ...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1385/BTER:78:1-3:113
更新日期：2000-01-01 00:00:00
abstract：:The objective of this paper is to investigate the correlation between serum macro-element and trace element contents and bone mineral density (BMD) as well as the occurrence of osteoporosis. After the epidemiological investigation of 290 postmenopausal women from ages 45 to 65 in the Xi'an urban area, their blood was ...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-009-8363-4
更新日期：2009-12-01 00:00:00
abstract：:The aim of the study has been to determine and compare the influence upon the kidney antioxidative system, exercised by administration of vitamin E, and vitamin E in combination with methionine, under conditions of oxidative stress induced by sodium fluoride. The experiment was carried out on Wistar FL rats (adult mal...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-007-8030-6
更新日期：2008-01-01 00:00:00
abstract：:Fruit juices are widely consumed in tropical countries as part of habitual diet. The concentrations of several minerals in these beverages were evaluated. Four commercially available brands of juices were analyzed for cadmium, lead, copper, zinc, aluminum, iron, chromium, manganese, and molybdenum. The levels ranged f...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-011-9247-y
更新日期：2012-05-01 00:00:00
abstract：:The aim of the present study was to examine the effects of exogenous selenium (Se) supplementation on the tolerance of pepper (Capsicum annuum L.) cv. Suryamukhi Cluster plants to cadmium (Cd) phytotoxicity at the reproductive stage. The pepper plants were supplied with Cd (0, 0.25 or 0.50 mM) and Se (0, 3 or 7 μM), i...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-014-0028-2
更新日期：2014-07-01 00:00:00
abstract：:Several epidemiological studies have shown that exposure to electromagnetic radiation (EMR) can be harmful to human health. The purpose of this study was to examine oxidative parameters and apoptosis induced by EMR in human kidney embryonic cells (HEK293) and to investigate whether zinc (Zn) has protective effect on E...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-019-01811-6
更新日期：2020-04-01 00:00:00
abstract：:In the mouse embryo cell line BALB/3T3 Clone A31-1-1, dose-dependent morphologic neoplastic transformation was obtained with NaAsO2, Na2HAsO4, CdCl2, and K2CrO4. Cellular uptake was four fold higher for As3+ than for As5+, and As5- was metabolized to As3+ in cytosol. Cytotoxicity and transformation rates were four fol...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02917291
更新日期：1989-07-01 00:00:00
abstract：:Oxidative stress is a main factor in the pathogenesis of severe acute pancreatitis (SAP). The ability of zinc (Zn) to retard oxidative processes has been recognized for many years. This study aims to examine the levels of free oxygen radicals and antioxidant enzyme in SAP rats and know the effect of Zn supplementation...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-014-9971-1
更新日期：2014-06-01 00:00:00
abstract：:This experiment was designed to investigate the histological and lipid peroxidation effects of chronic fluorosis on testes tissues of first- and second-generation rats. Sixteen virgin female Wistar rats were mated with eight males (2:1) for approximately 12 h to obtain first-generation rats. Pregnant rats were divided...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-007-0036-6
更新日期：2007-09-01 00:00:00
abstract：:The forms of Se in the Se-dependent enzyme formate dehydrogenase is known to be selenocysteine, but the way this amino acid enters the polypeptide chain has not been established. Through the use of a cysteine-requiring mutant ofEscherichia coli K-12 that could also grow in the presence of glutathione, we were able to ...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02795520
更新日期：1986-12-01 00:00:00
abstract：:China has eliminated iodine deficiency disorders since 2011 via the implementation of universal salt iodisation. Following this, a new revised salt iodisation policy was introduced to reduce iodine content in table salt. Since maternal iodine deficiency can lead to cognitive impairment and cretinism in infants, the ai...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-020-02135-6
更新日期：2021-01-01 00:00:00
abstract：:This meta-analysis was conducted aiming to summarize the results obtained from the previous studies so that the effect of opium on blood lead levels (BLLs) can be investigated. Scopus, Embase, PubMed, and Web of Science (ISI) were systematically searched up to June 2020. Heterogeneity of the included studies was evalu...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-020-02504-1
更新日期：2020-11-26 00:00:00
abstract：:Neutron activation analysis (NAA) methodology was applied for the certification of eight trace elements (As, Co, Cr, Cu, Hg, Mn, Se, and Zn) in a plankton reference material (CRM 414) prepared by the BCR of the Commission of the European Communities. Average concentrations (micrograms/g dry wt) and standard deviations...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02917365
更新日期：1994-10-01 00:00:00
abstract：:The efficacy of selenate and other divalent anions to stimulate the efflux of radiolabeled sulfate from lactating rat mammary tissue slices has been examined. Both selenate and sulfate markedly increased the fractional release of sulfate via a system that is temperature-sensitive and sensitive to the anion-exchange in...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02917205
更新日期：1990-03-01 00:00:00
