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Quantification of cellular uptake and in vivo tracking of transduction using real-time monitoring.

Abstract：

:Protein transduction domains (PTDs) are short amino acid sequences that promote their own translocation across the cell plasma membrane and have been studied for possible use in drug delivery and gene therapy. However, no direct method to quantify transduction is available. Here, using a new luciferase-tagged human PTD, we show that cellular uptake levels can be determined in a reliable manner. Furthermore, we show that enhanced in vivo tracking by human PTD can be quantified in a mouse model. This is the first report on the direct quantification of PTD transduction in vitro and in vivo, which will be necessary for studying its possible therapeutic application in drug delivery and gene therapy.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Lee MS,Kwon EH,Choi HS,Kwon SH,Lee CH,Shim IS,Lee SK,Her S

doi

10.1016/j.bbrc.2010.03.009

subject

Has Abstract

pub_date

2010-04-02 00:00:00

pages

348-53

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(10)00432-8

journal_volume

394

pub_type

杂志文章

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