Abstract:
PURPOSE:Robo1, a member of the roundabout (Robo) family, serves as neuronal guidance receptors and has been reported to mediate tumor angiogenesis. In this study, we investigated the function of Robo1 and its possible role in retinal angiogenesis in vitro and in vivo. METHODS:Expression of Robo1 in relation to retinal neovascularization was studied in an animal model of retinopathy of prematurity (ROP). siRNA technology was used to knockdown Robo1 expression to study its effects on monkey choroidal retinal endothelial cells (RF/6A) in vitro. Cell proliferation, migration, spreading, cycling, and apoptosis were assessed with the methyl thiazolyl tetrazolium (MTT), Boyden chamber, immunohistochemistry, and flow cytometry assay. Tube formation by RF/6A on Matrigel was also analyzed. RESULTS:Robo1 expression was elevated in the ROP murine eyes (P < 0.01). Knockdown of Robo1 expression inhibited cell proliferation and migration. Tube formation by RF/6A was also disturbed. CONCLUSIONS:Our results show that Robo1 inhibits choroidal and retinal angiogenesis in vitro. Further studies are ongoing to evaluate Robo1 as a potential target for the treatment of choroidal or retinal angiogenesis.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Huang L,Xu Y,Yu W,Li X,Liqun C,He X,Peiying Hdoi
10.3109/02713680903308495subject
Has Abstractpub_date
2009-12-01 00:00:00pages
1019-29issue
12eissn
0271-3683issn
1460-2202journal_volume
34pub_type
杂志文章abstract::The effect of electrical stimulation of the sympathetic nerves on the blood-aqueous barrier was investigated in rabbits. The permeability of the barrier was assessed during either acute, chronic or following chronic nerve stimulation. During acute and chronic stimulation of the sympathetic nerves, fluorescein entered ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689009069929
更新日期:1990-10-01 00:00:00
abstract::The corneal endothelium of alloxan diabetic dogs was examined by wide-field specular microscopy and compared to that of age-matched non-diabetic dogs. Computer-assisted morphometry of individual cells showed that the diabetic dog endothelium had marked polymegethism and pleomorphism similar to that previously describe...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688509020031
更新日期:1985-07-01 00:00:00
abstract::The changes in mouse corneal inflammatory cell population and the phagocytic capability of these cells were studied quantitatively at 24 and 48 hours after eliciting inflammatory cells into the cornea by Pseudomonas aeruginosa inoculation. Mice were selected for study according to their ability (young adult Swiss-Webs...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008995199
更新日期:1990-02-01 00:00:00
abstract:PURPOSE:The aim of this study was to characterize swelling pressure-thickness, swelling pressure-hydration and hydration-thickness relations of porcine cornea. METHODS:Mechanical compression tests and free swelling experiments were performed on porcine cornea. A rheometer (DHR-2, TA Instruments) with a thermally contr...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2013.809769
更新日期:2013-11-01 00:00:00
abstract:PURPOSE:Extracellular matrix homeostasis is dependent in part upon a family of matrix metalloproteinases and their specific inhibitors, the tissue inhibitors of metalloproteinases (TIMPs). Recently, gene defects in TIMP-3 have been identified in the affected individuals of several families with Sorsby's fundus dystroph...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.2.102.5086
更新日期:1997-02-01 00:00:00
abstract::The initial rapid fall in tear film fluorescence after instilling a 1 microliter drop of fluorophore could be a result of stimulated tear flow or of distribution of the dye throughout the tear fluid present in the conjunctival sac. An attempt was made to decide this by comparing the fall in fluorescence of two differe...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689509033526
更新日期:1995-04-01 00:00:00
abstract:PURPOSE:Corneal autofluorescence is related to advanced glycation end products formed by glucose that reaches the cornea via the aqueous humour. The aim of the study was to examine the influence on autofluorescence of changes in permeability of the blood aqueous barrier. METHODS:Corneal autofluorescence was measured i...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.26.4.307.15433
更新日期:2003-05-01 00:00:00
abstract:PURPOSE:To evaluate the effect of the birth weight and the postconceptional age on the tear production of preterm and term newborn infants and to evaluate the changes in tear production during the first two months of life. SUBJECTS--METHODS: Both eyes of medically stable term and preterm infants were included in the st...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.28.2.145.26235
更新日期:2004-02-01 00:00:00
abstract::The effects of light damage were studied in four inbred strains of albino rats, Fischer (F344), Lewis (L), Wistar (W), and Buffalo (BUF). Thirty-five-day-old rats (3 from each strain) were kept in cyclic light for two weeks, after which they were exposed to constant fluorescent light (180-190 foot-candles, 490-580 nm)...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008999576
更新日期:1990-08-01 00:00:00
abstract:PURPOSE:To evaluate how patient satisfaction after cataract surgery is associated with postoperative visual acuity, visual functioning, dry eye signs and symptoms, health anxiety, and depressive symptoms. PATIENTS AND METHODS:Fifty-four patients (mean age: 68.02 years) were assessed 2 months after uneventful phacoemul...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1262429
更新日期:2017-06-01 00:00:00
abstract:PURPOSE:To quantitatively measure the flow, nonflow, density, and foveal avascular zone (FAZ) assessment tool parameters of optical coherence tomography angiography (OCTA) in patients with nonocular Behcet's Disease (BD), and to compare the results with those obtained in healthy controls. METHODS:FAZ parameters, flow ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1530361
更新日期:2019-02-01 00:00:00
abstract::It has been shown that illumination of rod outer segment suspension in the presence of photosensitizers (methylene blue lambda greater than or equal to 620 nm; retinal 370 less than or equal to lambda less than or equal to 390 nm) results in chemical modification of the lipid and protein components of the photo-recept...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688209019997
更新日期:1982-01-01 00:00:00
abstract:PURPOSE:To investigate the relationship between meibomian gland dysfunction (MGD) and incomplete blinking caused by cranial nerve seven (CN VII) palsy. METHODS:A prospective case series of 60 consecutive patients with unilateral CN VII palsy was evaluated for MGD. According to the House-Brackmann scale, patients were ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1007211
更新日期:2016-01-01 00:00:00
abstract:PURPOSE:Disulfiram (DSF) exhibits a wide variety of biological activities, including an anti-inflammatory action, on which we focused our attention. The aim of the present study was to investigate the effect of oral DSF on endotoxin-induced uveitis (EIU) in rats. METHODS:We investigated its effect upon cellular infilt...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2010.495442
更新日期:2010-10-01 00:00:00
abstract::The intraocular inflammatory reaction in a murine model of herpes simplex keratouveitis was studied using immunohistochemical techniques. 5 x 10(5) PFU of HSV-1 (Stewart strain) was applied to the abraded cornea of BALB/c mice. The subsequent development of keratouveitis was documented by sequential examination, conve...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688909013892
更新日期:1989-01-01 00:00:00
abstract:PURPOSE:To investigate orbital biomechanical properties in patients with thyroid orbitopathy and in age- and gender-matched healthy subjects using the Corvis-ST (CST, Oculus Wetzlar, Germany). METHODS:The CST allows a non-contact tonometry with an ultra-high-speed Scheimpflug device to record the deformation of the co...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1405044
更新日期:2018-03-01 00:00:00
abstract::Langerhans cells (LC) appear to have a role in the processing of antigens presented through cutaneous surfaces. In most species LC are found in the conjunctiva and limbal epithelium but are rarely found in the central cornea. A quantitative study of corneal LC was performed in guinea pigs and rabbits with HSV-1 kerati...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709020087
更新日期:1987-01-01 00:00:00
abstract::Free radicals and oxidative damage play roles in aging and age-related ocular diseases such as cataracts, so defensive mechanisms become important factors for protection. Because N-acetylation is involved in a wide variety of detoxification processes, this study was conducted to examine the relationship between the ac...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680591003799
更新日期:2005-10-01 00:00:00
abstract::Lenses from normal Wistar rats and those from a Wistar strain with X-ray-induced cataract mutation were examined electron microscopically for morphological characterization and acid phosphatase (AcPase) localization. Ocular anlages of 11 through 18 days of gestation were included in the study. A late-separating or per...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409003064
更新日期:1984-04-01 00:00:00
abstract:PURPOSE:This study was performed to compare the positive predictive value of peripapillary retinal nerve fiber layer (RNFL) thickness measurements obtained using Cirrus high-definition optical coherence tomography (Cirrus HD-OCT; Carl Zeiss Meditec, Dublin, CA) and spectral OCT/scanning laser ophthalmoscopy (SLO) (OPKO...
journal_title:Current eye research
pub_type: 临床试验,杂志文章
doi:10.3109/02713683.2013.824989
更新日期:2014-01-01 00:00:00
abstract:PURPOSE:Matrix metalloproteinases (MMPs) are involved in posterior capsule opacification (PCO), but the mechanisms that promote MMP expression are yet to be determined. In this study, we investigated whether type I collagen, which is only detected in aged or cataractous lens capsules, affects the expression and activat...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2013.853194
更新日期:2014-05-01 00:00:00
abstract::Four groups of BALB/c mice were treated locally with different concentrations (0.1-1.0%) of retinoic acid (Etretinate) ointment for a period of one to four weeks. A fifth group was treated with the ointment base only. Ia-positive dendritic cells were identified by an indirect immunofluorescence technique which was con...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689509033511
更新日期:1995-03-01 00:00:00
abstract:OBJECTIVE:Children with chronic kidney diseases (CKD) are at risk for neurological diseases at early adulthood. Spectral-domain optical coherence tomography (SD-OCT) of the retina is especially suitable for determination of intraretinal layer thickness. We wonder whether retinal thinning is already present in pediatric...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1522649
更新日期:2019-02-01 00:00:00
abstract:PURPOSE:Ethacrynic acid (ECA) has been shown to increase facility of aqueous outflow in whole eyes and perfused anterior segments, to open up spaces between cells in the trabecular meshwork and inner wall of Schlemm's canal, and to cause separation and retraction of trabecular meshwork and endothelial cells in culture....
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689609017644
更新日期:1996-09-01 00:00:00
abstract::The efficiency of protein immobilization to provide a hydrogel surface with a high affinity for corneal epithelial cells was investigated. The cell adhesive proteins collagen and fibronectin, and the cell adhesive oligopeptide RGDS (arginine-glycine-aspartic acid-serine) were successfully covalently immobilized on pol...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689109020325
更新日期:1991-10-01 00:00:00
abstract::Two different measures of response to therapy were evaluated in a model of keratitis caused by Aspergillus fumigatus in Dutch-belted rabbits. Combined pre and post-inoculation treatment with oral fluconazole 37.5 mg/kg bid or itraconazole 40 mg/kg bid was compared to post-inoculation treatment only and untreated contr...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689209069165
更新日期:1992-01-01 00:00:00
abstract::Utilizing a rapid colorimetric assay that relates cell number to cytoplasmic hexosaminidase activity, we conducted drug-induced cytotoxicity experiments on human ocular fibroblasts cultured from Tenon's capsule specimens. The effects of two different agents--dimethyl sulfoxide (DMSO) or mitomycin C--on the proliferati...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008999599
更新日期:1990-06-01 00:00:00
abstract::Purpose: Notch signaling plays a vital role in the differentiation and proliferation of corneal epithelial cells from limbal stem cells. The temporal regulation of Notch signaling during this differentiation remains unknown. Hence, we investigated the importance of temporal activation/blockage of Notch signaling durin...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1673436
更新日期:2020-04-01 00:00:00
abstract::Objectives: To investigate the characteristics of margin reflex distance 1 (MRD1) asymmetry in congenital lower eyelid epiblepharon and its resolution after surgical correction of epiblepharon. Methods: Among patients who underwent lower eyelid epiblepharon surgery from November 2015 to September 2017, patients with a...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1857781
更新日期:2020-12-15 00:00:00
abstract:PURPOSE:The mechanism by which prostaglandin(PG)F2 alpha increases uveoscleral outflow and lowers intraocular pressure in primates is not known. In cultured human ciliary muscle cells, PGF2 alpha induces the expression of the protooncogene c-fos which is known to induce the transcription of genes such as matrix metallo...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689609017628
更新日期:1996-08-01 00:00:00