Ultrastructural localization of c-erbB-2 gene product in transitional cell carcinoma of the urinary tract.

Abstract:

:The c-erbB-2 gene product was demonstrated immunoelectron microscopically in human transitional cell carcinomas of the urinary tract. Two antibodies specific for the c-erbB-2 gene product were used: a polyclonal antibody directed to the intracellular domain and a monoclonal antibody recognizing an epitope in the extracellular domain. The immunoreaction with the polyclonal antibody was observed on cell membranes and the cytoplasmic area beneath membranes with particularly strong reaction on microvilli. The immune reaction with the monoclonal antibody was confined to cell membranes with a linear pattern. Amorphous deposits in the intercellular spaces were also immunoreactive with the monoclonal antibody directed to an extracellular epitope, suggesting that a part of the c-erbB-2 gene product may be released from the cancer cell membranes into the extracellular space.

journal_name

Ultrastruct Pathol

authors

Moriyama M,Morishita Y,Mori S,Akiyama T,Kato T

doi

10.3109/01913129009007219

subject

Has Abstract

pub_date

1990-09-01 00:00:00

pages

399-405

issue

5

eissn

0191-3123

issn

1521-0758

journal_volume

14

pub_type

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