Synchronous plasma membrane electrochemical potential oscillations during yeast colony development and aging.

abstract：:2B is a 99 amino acid membrane protein encoded by enteroviruses such as polio and coxsackie viruses with two transmembrane domains. The protein is found to make membranes of infected cells permeable. Using a computational approach which positions the models and assesses stability by molecular dynamics (MD) simulations...

journal_title：Molecular membrane biology

authors： Patargias G,Barke T,Watts A,Fischer WB

更新日期：2009-08-01 00:00:00

abstract：:Organellar and cytosolic pH homeostasis is central to most cellular processes, including vesicular trafficking, post-translational modification/processing of proteins, and receptor-ligand interactions. SLC9A7 (NHE7) was identified as a unique (Na+, K+)/H+ exchanger that dynamically cycles between the trans-Golgi netwo...

journal_title：Molecular membrane biology

authors： Kagami T,Chen S,Memar P,Choi M,Foster LJ,Numata M

更新日期：2008-08-01 00:00:00

abstract：:Nedd4 family ubiquitin ligases regulate trafficking and degradation of numerous target substrates in different cellular compartments, including at the plasma membrane, in endosomes, in the secretory pathway and in the nucleus. WWP1 is a Nedd4 family protein closely related to mouse Itch and Drosophila Su(dx), both of ...

journal_title：Molecular membrane biology

authors： Flasza M,Nguyen Huu NS,Mazaleyrat S,Clémence S,Villemant C,Clarke R,Baron M

更新日期：2006-05-01 00:00:00

abstract：:At least four different types of interaction between protein transmembrane helices have been described to date. These include the use of charge-pair interactions that can play a positive or negative role in the assembly of multi-subunit complexes such as the T cell receptor, or recruit signal transducing accessory mol...

journal_title：Molecular membrane biology

authors： Harrison PT

更新日期：1996-04-01 00:00:00

abstract：:Site-directed mutagenesis and site-directed fluorescence spectroscopy demonstrate that Cys148 interacts hydrophobically with the galactosyl moiety of substrates of the lactose permease of Escherichia coli. By taking advantage of the finding that labelling of single-Cys148 permease with the thiol-specific fluorophore 2...

journal_title：Molecular membrane biology

authors： He MM,Kaback HR

更新日期：1998-01-01 00:00:00

abstract：:Membrane proteins are intrinsically involved in both human and pathogen physiology, and are the target of 60% of all marketed drugs. During the past decade, advances in the studies of membrane proteins using X-ray crystallography, electron microscopy and NMR-based techniques led to the elucidation of over 250 unique m...

journal_title：Molecular membrane biology

authors： Simmons KJ,Gotfryd K,Billesbølle CB,Loland CJ,Gether U,Fishwick CW,Johnson AP

更新日期：2013-03-01 00:00:00

abstract：:A group of prenyltransferases produce linear lipids by catalyzing consecutive condensation reactions of farnesyl diphosphate (FPP) with specific numbers of isopentenyl diphosphate (IPP), a common building block of isoprenoid compounds. Depending on the stereochemistry of the double bonds formed during IPP condensation...

journal_title：Molecular membrane biology

authors： Teng KH,Liang PH

更新日期：2012-11-01 00:00:00

abstract：:Eleven sequenced anion exchanger (AE; band 3) proteins, including five AE1, four AE2 and two AE3 proteins, comprise the anion exchanger family (AEF) of homologous proteins. Eliminating the rat and rabbit proteins that are nearly identical to the corresponding mouse proteins, seven dissimilar members of this family wer...

journal_title：Molecular membrane biology

authors： Espanol MJ,Saier MH Jr

更新日期：1995-04-01 00:00:00

abstract：:The transverse distribution of phosphatidylglycerol (PG) across the inner membrane of Escherichia coli was studied. The oxidation of PG by periodate was used to discriminate between the PG present in the cytoplasmic and in the periplasmic leaflet of the inner membrane. Applied to large unilamellar vesicles derived of ...

journal_title：Molecular membrane biology

authors： Huijbregts RP,de Kroon AI,de Kruijff B

更新日期：1997-01-01 00:00:00

abstract：:Temperature-composition phase diagrams of binary lipid mixtures under conditions of constant pressure and excess water show that component immiscibility is the rule rather than the exception in these systems. Phase immiscibility is particularly noted in the solid phase but several cases are known where immiscibility i...

journal_title：Molecular membrane biology

authors： Vaz WL

更新日期：1995-01-01 00:00:00

abstract：:Small GTPase Rab36 is homologous to Rab34 with 56% amino acid sequence identity. Rab34 was characterized as a Golgi-associated Rab protein and regulates lysosomal positioning through interaction with RILP; however, the properties and functions of Rab36 have not been investigated. To investigate Rab36, we constructed E...

journal_title：Molecular membrane biology

authors： Chen L,Hu J,Yun Y,Wang T

更新日期：2010-01-01 00:00:00

abstract：:NupG from Escherichia coli is the archetype of a family of nucleoside transporters found in several eubacterial groups and has distant homologues in eukaryotes, including man. To facilitate investigation of its molecular mechanism, we developed methods for expressing an oligohistidine-tagged form of NupG both at high ...

journal_title：Molecular membrane biology

authors： Xie H,Patching SG,Gallagher MP,Litherland GJ,Brough AR,Venter H,Yao SY,Ng AM,Young JD,Herbert RB,Henderson PJ,Baldwin SA

更新日期：2004-09-01 00:00:00

abstract：:Palmitoylation is required for the activities of several cancer-associated proteins, making the palmitoyl acyltransferase (PAT) enzymes that catalyze these reactions potential targets for anticancer therapeutics. In this study, we sought to identify and characterize a human PAT with activity toward N-terminally myrist...

journal_title：Molecular membrane biology

authors： Draper JM,Smith CD

更新日期：2010-04-01 00:00:00

abstract：:Radiation-based therapies aided by nanoparticles have been developed for decades, and can be primarily categorized into two main platforms. First, delivery of payload of photo-reactive drugs (photosensitizers) using the conventional nanoparticles, and second, design and development of photo-triggerable nanoparticles (...

journal_title：Molecular membrane biology

authors： Yavlovich A,Smith B,Gupta K,Blumenthal R,Puri A

更新日期：2010-10-01 00:00:00

abstract：:Evidence was recently reported that the cysteine proteinase inhibitor, cystatin C, is highly expressed by cultured human retinal pigment epithelial (RPE) cells. As a step towards understanding possible functions of this protein associated with the RPE, the localization, targetting and trafficking of cystatin C were in...

journal_title：Molecular membrane biology

authors： Paraoan L,White MR,Spiller DG,Grierson I,Maden BE

更新日期：2001-07-01 00:00:00

abstract：:We have previously shown that a mixture of cerebrosides obtained from dried tubers of herb Typhonium giganteum Engl. plays a neuroprotective role in the ischemic brain through its effect on activation of BK(Ca) channels. It is very curious to know whether a single pure cerebroside compound could activate the BK(Ca) ch...

journal_title：Molecular membrane biology

authors： Xu H,Qi J,Wang G,Deng H,Qi Z

更新日期：2011-02-01 00:00:00

abstract：:The peptide specificity of both presequence-monolayer interactions and the ability of presequences to induce interbilayer contacts between large unilamellar vesicles was investigated. A range of different synthetic peptides that are documented for their mitochondrial protein import abilities were used for this purpose...

journal_title：Molecular membrane biology

authors： Leenhouts JM,Török Z,Demel RA,de Gier J,de Kruijff B

更新日期：1994-07-01 00:00:00

abstract：:The Signal Recognition Particle (SRP) plays a critical role in the sorting of nascent secretory and membrane proteins. Remarkably, this function has been conserved from bacteria, where SRP delivers proteins to the inner membrane, through to eukaryotes, where SRP is required for targeting of proteins to the endoplasmic...

journal_title：Molecular membrane biology

authors： Pool MR

更新日期：2005-01-01 00:00:00

abstract：:Homotypic fusion between early endosomes can be reconstituted in vitro. By using wortmannin and LY294002, inhibitors of phosphatidylinositol (Pl) 3-kinase, a requirement for this activity has been established in order for fusion to proceed efficiently. It has been shown that Pl 3-kinase activity is required downstream...

journal_title：Molecular membrane biology

authors： Mills IG,Jones AT,Clague MJ

更新日期：1999-01-01 00:00:00

abstract：:Membrane microdomains enriched in cholesterol, sphingolipids (rafts), and specific proteins are involved in important physiological functions. However their structure, size and stability are still controversial. Given that detergent-resistant membranes (DRMs) are in the liquid-ordered state and are rich in raft-like c...

journal_title：Molecular membrane biology

authors： Casadei BR,De Oliveira Carvalho P,Riske KA,Barbosa Rde M,De Paula E,Domingues CC

更新日期：2014-09-01 00:00:00

abstract：:Calpain, an intracellular calcium-dependent protease, is activated at cell membranes and cleaves cytoskeletal and submembranous proteins. Calpain is inferred to be a calcium-dependent regulator for cytoskeletal reorganization. Calpastatin, an endogenous calpain inhibitor, inhibits not only the proteolytic activity of ...

journal_title：Molecular membrane biology

authors： Kawasaki H,Kawashima S

更新日期：1996-10-01 00:00:00

abstract：:'No cellular organelle has been the subject of as many, as long-lasting or as diverse polemics as the Golgi apparatus'. This statement was made by Whaley almost 30 years ago in the book The Golgi Apparatus and still holds true today, perhaps more then ever. Why is this? How come something as mundane as a series of int...

journal_title：Molecular membrane biology

authors： Elsner M,Hashimoto H,Nilsson T

更新日期：2003-07-01 00:00:00

abstract：:Lipid rafts are specialized plasma membrane microdomains in which glycosphingolipids and cholesterol are major structural components. Their relative insolubility to nonionic detergents is the most widely used method to purify these structures. Several signalling proteins are associated with these microdomains in T lym...

journal_title：Molecular membrane biology

authors： Salgado FJ,Piñeiro A,Canda-Sánchez A,Lojo J,Nogueira M

更新日期：2005-05-01 00:00:00

abstract：:The ATP binding cassette (ABC) transporter Atm1p of the mitochondrial inner membrane performs crucial roles in both the biogenesis of cytosolic/nuclear iron-sulfur proteins and cellular iron homeostasis. Since the function of the mitochondrial iron-sulfur cluster (ISC) assembly machinery is also required for these two...

journal_title：Molecular membrane biology

authors： Kuhnke G,Neumann K,Mühlenhoff U,Lill R

更新日期：2006-03-01 00:00:00

abstract：:In Saccharomyces cerevisiae Jen1p is a lactate/proton symporter belonging to the lactate/pyruvate:H(+) symporter subfamily (TC#2.A.1.12.2) of the Major Facilitator Superfamily. We investigated structure-function relationships of Jen1p using a rational mutational analysis based on the identification of conserved amino ...

journal_title：Molecular membrane biology

authors： Soares-Silva I,Paiva S,Diallinas G,Casal M

更新日期：2007-09-01 00:00:00

abstract：:Gap junctions are plasma membrane spatial microdomains constructed of assemblies of channel proteins called connexins in vertebrates and innexins in invertebrates. The channels provide direct intercellular communication pathways allowing rapid exchange of ions and metabolites up to approximately 1 kD in size. Approxim...

journal_title：Molecular membrane biology

authors： Evans WH,Martin PE

更新日期：2002-04-01 00:00:00

abstract：:Neurotransmitter transporters are regulated through a variety of signal transduction mechanisms which appear to operate in order to maintain appropriate levels of transmitter in the synaptic cleft. One such mechanism is the trafficking of the transporter in association with synaptic vesicle release machinery. This rep...

journal_title：Molecular membrane biology

authors： Horton N,Quick MW

更新日期：2001-01-01 00:00:00

abstract：:Trypsin activation of Cry4B, a 130-kDa Bacillus thuringiensis (Bt) protein, produces a 65-kDa toxin active against mosquito larvae. The active toxin is made of two protease resistant-products of ca. 45 kDa and ca. 20 kDa. The cloned 21-kDa fragment consisting of the N-terminal region of the toxin was previously shown ...

journal_title：Molecular membrane biology

authors： Puntheeranurak T,Uawithya P,Potvin L,Angsuthanasombat C,Schwartz JL

更新日期：2004-01-01 00:00:00

abstract：:Previous work from our laboratory has established bovine hippocampal membranes as a convenient natural source for studying neuronal receptors such as the G-protein coupled serotonin1A receptor. In this paper, we have explored the organization and dynamics of bovine hippocampal membranes using environment-sensitive and...

journal_title：Molecular membrane biology

authors： Mukherjee S,Kalipatnapu S,Pucadyil TJ,Chattopadhyay A

更新日期：2006-09-01 00:00:00

abstract：:The mitochondrial ADP/ATP carrier imports ADP from the cytosol into the mitochondrial matrix for its conversion to ATP by ATP synthase and exports ATP out of the mitochondrion to replenish the eukaryotic cell with chemical energy. Here the substrate specificity of the human mitochondrial ADP/ATP carrier AAC1 was deter...

journal_title：Molecular membrane biology

authors： Mifsud J,Ravaud S,Krammer EM,Chipot C,Kunji ER,Pebay-Peyroula E,Dehez F

更新日期：2013-03-01 00:00:00