Abstract:
:Calpain, an intracellular calcium-dependent protease, is activated at cell membranes and cleaves cytoskeletal and submembranous proteins. Calpain is inferred to be a calcium-dependent regulator for cytoskeletal reorganization. Calpastatin, an endogenous calpain inhibitor, inhibits not only the proteolytic activity of calpain but also the binding of calpain to membranes. Calpain activity is strictly regulated by calcium and calpastatin. Calpain has two distinct sites for interaction with calpastatin, one the active site and the other an EF-hand domain. It is believed that calpain interacts with substrates through the same two sites. We discuss the regulation of membrane binding and the activity of calpain through these two sites.
journal_name
Mol Membr Bioljournal_title
Molecular membrane biologyauthors
Kawasaki H,Kawashima Sdoi
10.3109/09687689609160599subject
Has Abstractpub_date
1996-10-01 00:00:00pages
217-24issue
4eissn
0968-7688issn
1464-5203journal_volume
13pub_type
杂志文章,评审abstract::Cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-regulated C1(-) channel. Malfunction of CFTR causes cystic fibrosis (CF). CFTR belongs to an ATP-binding cassette (ABC) transporter superfamily which includes P-glycoprotein (Pgp), the molecule that is responsible for multidrug resistance in cancer c...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689609160572
更新日期:1996-01-01 00:00:00
abstract::Nedd4 family ubiquitin ligases regulate trafficking and degradation of numerous target substrates in different cellular compartments, including at the plasma membrane, in endosomes, in the secretory pathway and in the nucleus. WWP1 is a Nedd4 family protein closely related to mouse Itch and Drosophila Su(dx), both of ...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860600665010
更新日期:2006-05-01 00:00:00
abstract::Notch receptor signalling plays a central role in development and its misfunction has been linked to a number of diseases. In the cannonical Notch signalling pathway, ligand binding to Notch activates a series of proteolytic cleavages that release the Notch intracellular domain for trafficking to the nucleus, where it...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/09687860500129778
更新日期:2005-07-01 00:00:00
abstract::Evidence was recently reported that the cysteine proteinase inhibitor, cystatin C, is highly expressed by cultured human retinal pigment epithelial (RPE) cells. As a step towards understanding possible functions of this protein associated with the RPE, the localization, targetting and trafficking of cystatin C were in...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680110075101
更新日期:2001-07-01 00:00:00
abstract::Constitutive secretion is used to deliver newly synthesized proteins to the cell surface and to the extracellular milieu. The trans-Golgi network is a key station along this route that mediates sorting of proteins into distinct transport pathways, aided in part by clathrin and adaptor proteins. Subsequent movement of ...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/0968768031000084172
更新日期:2003-04-01 00:00:00
abstract::2B is a 99 amino acid membrane protein encoded by enteroviruses such as polio and coxsackie viruses with two transmembrane domains. The protein is found to make membranes of infected cells permeable. Using a computational approach which positions the models and assesses stability by molecular dynamics (MD) simulations...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680903164101
更新日期:2009-08-01 00:00:00
abstract::A group of prenyltransferases produce linear lipids by catalyzing consecutive condensation reactions of farnesyl diphosphate (FPP) with specific numbers of isopentenyl diphosphate (IPP), a common building block of isoprenoid compounds. Depending on the stereochemistry of the double bonds formed during IPP condensation...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687688.2012.674162
更新日期:2012-11-01 00:00:00
abstract::At least four different types of interaction between protein transmembrane helices have been described to date. These include the use of charge-pair interactions that can play a positive or negative role in the assembly of multi-subunit complexes such as the T cell receptor, or recruit signal transducing accessory mol...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687689609160579
更新日期:1996-04-01 00:00:00
abstract::The serotonin1A (5-HT1A) receptors are members of a superfamily of seven transmembrane domain receptors that couple to G-proteins. They appear to be involved in various behavioural and cognitive functions. This paper reports an efficient strategy to solubilize 5-HT1A receptors from bovine hippocampal membranes using t...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680210149586
更新日期:2002-07-01 00:00:00
abstract::The entry of substrates into, and the export of glururonides from, the lumen of hepatic endoplasmic reticulum (ER) in vitro (sealed microsomes) has been measured using radioactivity-labelled materials and a rapid filtration assay. Analysis of liver microsomes from a jaundiced patient showed the accumulation of bilirub...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689509072429
更新日期:1995-07-01 00:00:00
abstract::A lactate permease was biochemically identified in Candida albicans RM1000 presenting the following kinetic parameters at pH 5.0: Km 0.33+/-0.09 mM and Vmax 0.85+/-0.06 nmol s(-1) mg dry wt(-1). Lactate uptake was competitively inhibited by pyruvic and propionic acids; acetic acid behaved as a non-competitive substrat...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860400011373
更新日期:2004-11-01 00:00:00
abstract::Small GTPase Rab36 is homologous to Rab34 with 56% amino acid sequence identity. Rab34 was characterized as a Golgi-associated Rab protein and regulates lysosomal positioning through interaction with RILP; however, the properties and functions of Rab36 have not been investigated. To investigate Rab36, we constructed E...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687680903417470
更新日期:2010-01-01 00:00:00
abstract::NupG from Escherichia coli is the archetype of a family of nucleoside transporters found in several eubacterial groups and has distant homologues in eukaryotes, including man. To facilitate investigation of its molecular mechanism, we developed methods for expressing an oligohistidine-tagged form of NupG both at high ...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860400003941
更新日期:2004-09-01 00:00:00
abstract::The effect of cholesterol and lanosterol on the formation of structures in the gel/subgel phase of 1,2-dipalmitoylphosphatidylcholine was investigated using freeze-fracture electron microscopy and X-ray diffraction. Mixtures with up to 25 mol% sterol were analysed after annealing for between several days and some mont...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689709048181
更新日期:1997-10-01 00:00:00
abstract::Palmitoylation is required for the activities of several cancer-associated proteins, making the palmitoyl acyltransferase (PAT) enzymes that catalyze these reactions potential targets for anticancer therapeutics. In this study, we sought to identify and characterize a human PAT with activity toward N-terminally myrist...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687681003616854
更新日期:2010-04-01 00:00:00
abstract::In hair cells of the inner ear, phosphatidylserine (PS), detected with fluorescent annexin V labeling, was rapidly exposed on the external leaflet of apical plasma membranes upon dissection of the organ of Corti. PS externalization was unchanged by caspase inhibition, suggesting that externalization did not portend ap...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860600926883
更新日期:2007-01-01 00:00:00
abstract::PagP is a bacterial outer membrane protein consisting of an 8 stranded transmembrane beta-barrel and an N-terminal alpha-helix. It is an enzyme which catalyses transfer of a palmitoyl chain from a phospholipid to lipid A. Molecular dynamics simulations have been used to compare the dynamic behaviour in simulations sta...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680902788967
更新日期:2009-05-01 00:00:00
abstract::Spontaneous membrane adsorption, folding and insertion of the synthetic WALP16 and KALP16 peptides was studied by computer simulations starting from completely extended conformations. The peptides were simulated using an unmodified all-atom force field in combination with an efficient Monte Carlo sampling algorithm. T...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680802020313
更新日期:2008-04-01 00:00:00
abstract::Homotypic fusion between early endosomes can be reconstituted in vitro. By using wortmannin and LY294002, inhibitors of phosphatidylinositol (Pl) 3-kinase, a requirement for this activity has been established in order for fusion to proceed efficiently. It has been shown that Pl 3-kinase activity is required downstream...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/096876899294788
更新日期:1999-01-01 00:00:00
abstract::The cyanelles of the glaucocystophyte alga Cyanophora paradoxa resemble endosymbiotic cyanobacteria in morphology, pigmentation and, especially, in the presence of a peptidoglycan wall situated between the inner and outer envelope membranes. However, it is now clear that cyanelles in fact are primitive plastids. Phylo...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/09687860500041411
更新日期:2005-01-01 00:00:00
abstract::This investigation is aimed at defining the structural requirements for aliphatic polyamines to interact with mitochondrial binding sites, which are relevant for the regulation of the permeability transition and for mitochondrial polyamine uptake. The triamines N-aminoethyl-1,2-diaminoethane (dien) and N-aminopropyl-1...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680310001654916
更新日期:2004-03-01 00:00:00
abstract::Flavonoids are ubiquitous polyphenolic compounds, found in vascular plants, which are endowed with a large variety of biological effects. Some of these effects have been assumed to result from interactions with the cell plasma membrane. In order to investigate the nature of these interactions a fluorescence study was ...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/096876899294616
更新日期:1999-04-01 00:00:00
abstract::The assessment of the transverse distribution and mobility of NBD-labelled phospholipid analogues in biological membranes by selective chemical destruction of fluorescent label in the outer monolayer with dithionite has been investigated using resealed erythrocyte ghosts as a model system. The distribution of those an...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689409161028
更新日期:1994-01-01 00:00:00
abstract::Modification of proteins with the lipid palmitate, a process called palmitoylation, is important for the normal function of neuronal cells. However, most attention has focused on how palmitoylation regulates the targeting and trafficking of neurotransmitter receptors and non-enzymatic scaffold proteins. In this review...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/09687688.2016.1182652
更新日期:2015-08-01 00:00:00
abstract::The Signal Recognition Particle (SRP) plays a critical role in the sorting of nascent secretory and membrane proteins. Remarkably, this function has been conserved from bacteria, where SRP delivers proteins to the inner membrane, through to eukaryotes, where SRP is required for targeting of proteins to the endoplasmic...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/09687860400026348
更新日期:2005-01-01 00:00:00
abstract::Lipid rafts are specialized plasma membrane microdomains in which glycosphingolipids and cholesterol are major structural components. Their relative insolubility to nonionic detergents is the most widely used method to purify these structures. Several signalling proteins are associated with these microdomains in T lym...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860500063506
更新日期:2005-05-01 00:00:00
abstract::Treatment of BHK fibroblasts with V. cholerae sialidase for 20 min caused the breakdown of about 70% of total cellular ganglioside GM3 and the production of an approximately equivalent amount of lactosylceramide. On removal of the enzyme, a slow resynthesis of GM3 from lactosylceramide was observed, equivalent to abou...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689709044325
更新日期:1998-10-01 00:00:00
abstract::Caveolae are approximately 50-100 nm membrane micro-invaginations associated with the plasma membrane of a wide variety of cells. Although they were first identified in transmission electron micrographs approximately 40 years ago, their exact function(s) has remained controversial. Two well-established functions inclu...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687689509038506
更新日期:1995-01-01 00:00:00
abstract::Solid-state NMR combined with sample deuteration was used to probe the proximity of the low-affinity substrate D-glucose to its binding site within the Escherichia coli sugar transport protein GalP. Samples of E. coli inner membranes with amplified expression of GalP were incubated in D(2)O with D-[(13)C(6)]glucose an...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2012.743193
更新日期:2013-03-01 00:00:00
abstract::Absorption of dietary fat in the small intestine involves epithelial exposure to potentially harmful molecules such as bile salts and free fatty acids. We used organ culture of porcine jejunal explants incubated with a pre-digested mixture of fat (plant oil), bile and pancreatin to mimick the physiological process of ...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2013.780642
更新日期:2013-05-01 00:00:00