Abstract:
:The assessment of the transverse distribution and mobility of NBD-labelled phospholipid analogues in biological membranes by selective chemical destruction of fluorescent label in the outer monolayer with dithionite has been investigated using resealed erythrocyte ghosts as a model system. The distribution of those analogues can be determined in < 30 s directly in the cell suspension provided the permeation of dithionite across the membrane is suppressed. The results were compared with data on translocation of either NBD- or spin-labelled phospholipid analogues obtained with the technique of back exchange to BSA. It is shown that the passage of dithionite can be mediated by anion-transport systems such as band 3 which is inhibited by DIDS. Appropriate conditions for the applicability of the assay were elucidated also using resealed ghosts having fluorescent NBD-taurine in the intracellular lumen. The application of the assay to measure fast translocation processes, e.g. those mediated by the aminophospholipid translocase, is described.
journal_name
Mol Membr Bioljournal_title
Molecular membrane biologyauthors
Pomorski T,Herrmann A,Zachowski A,Devaux PF,Müller Pdoi
10.3109/09687689409161028subject
Has Abstractpub_date
1994-01-01 00:00:00pages
39-44issue
1eissn
0968-7688issn
1464-5203journal_volume
11pub_type
杂志文章abstract::Glycosyl-phosphatidylinositol (GPI)-anchored proteins are enriched in cholesterol- and sphingolipid-rich lipid rafts within the membrane. Rafts are known to have roles in cellular organization and function, but little is understood about the factors controlling the distribution of proteins in rafts. We have used atomi...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860601127770
更新日期:2007-05-01 00:00:00
abstract::Trypsin activation of Cry4B, a 130-kDa Bacillus thuringiensis (Bt) protein, produces a 65-kDa toxin active against mosquito larvae. The active toxin is made of two protease resistant-products of ca. 45 kDa and ca. 20 kDa. The cloned 21-kDa fragment consisting of the N-terminal region of the toxin was previously shown ...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680310001625792
更新日期:2004-01-01 00:00:00
abstract::This review describes the numerous and innovative methods used to study the structure and function of viral fusion peptides. The systems studied include both intact fusion proteins and synthetic peptides interacting with model membranes. The strategies and methods include dissecting the fusion process into intermediat...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687689709048170
更新日期:1997-07-01 00:00:00
abstract::Palmitoylation is required for the activities of several cancer-associated proteins, making the palmitoyl acyltransferase (PAT) enzymes that catalyze these reactions potential targets for anticancer therapeutics. In this study, we sought to identify and characterize a human PAT with activity toward N-terminally myrist...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687681003616854
更新日期:2010-04-01 00:00:00
abstract::4-Hydroxy-2-nonenal (4-HNE) is a reactive aldehyde and a lipid peroxidation product formed in biological tissues under physiological and pathological conditions. Its concentration increases with oxidative stress and induces deleterious modifications of proteins and membranes. Mitochondrial and cytosolic isoforms of cr...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2015.1023376
更新日期:2015-01-01 00:00:00
abstract::Site-directed mutagenesis and site-directed fluorescence spectroscopy demonstrate that Cys148 interacts hydrophobically with the galactosyl moiety of substrates of the lactose permease of Escherichia coli. By taking advantage of the finding that labelling of single-Cys148 permease with the thiol-specific fluorophore 2...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689809027513
更新日期:1998-01-01 00:00:00
abstract::The molecular events and the protein components that are involved in signalling by the T cell receptor (TCR) for antigen have been extensively studied. Activation of signalling cascades following TCR stimulation depends on the phosphorylation of the receptor by the tyrosine kinase Lck, which localizes to the cytoplasm...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/09687860500453673
更新日期:2006-01-01 00:00:00
abstract::Recent advances have revealed much about the signaling events and molecular components associated with autophagy. Although it is clear that there are multiple points of intersection and connection between autophagy and known vesicular membrane transport processes between membrane compartments, autophagy is modulated b...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2013.850178
更新日期:2013-12-01 00:00:00
abstract::The mitochondrial ADP/ATP carrier imports ADP from the cytosol into the mitochondrial matrix for its conversion to ATP by ATP synthase and exports ATP out of the mitochondrion to replenish the eukaryotic cell with chemical energy. Here the substrate specificity of the human mitochondrial ADP/ATP carrier AAC1 was deter...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2012.745175
更新日期:2013-03-01 00:00:00
abstract::CitS of Klebsiella pneumoniae and GltS of Escherichia coli are Na+-dependent secondary transporters from different families that are believed to share the same fold and quaternary structure. A 10 kDa protein tag (Biotin Acceptor Domain [BAD]) was fused to the N-terminus of both proteins (CitS-BAD1 and GltS-BAD1, respe...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2011.581252
更新日期:2011-08-01 00:00:00
abstract::Small GTPase Rab36 is homologous to Rab34 with 56% amino acid sequence identity. Rab34 was characterized as a Golgi-associated Rab protein and regulates lysosomal positioning through interaction with RILP; however, the properties and functions of Rab36 have not been investigated. To investigate Rab36, we constructed E...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687680903417470
更新日期:2010-01-01 00:00:00
abstract::Nedd4 family ubiquitin ligases regulate trafficking and degradation of numerous target substrates in different cellular compartments, including at the plasma membrane, in endosomes, in the secretory pathway and in the nucleus. WWP1 is a Nedd4 family protein closely related to mouse Itch and Drosophila Su(dx), both of ...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860600665010
更新日期:2006-05-01 00:00:00
abstract::Membrane microdomains enriched in cholesterol, sphingolipids (rafts), and specific proteins are involved in important physiological functions. However their structure, size and stability are still controversial. Given that detergent-resistant membranes (DRMs) are in the liquid-ordered state and are rich in raft-like c...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2014.949319
更新日期:2014-09-01 00:00:00
abstract::At least four different types of interaction between protein transmembrane helices have been described to date. These include the use of charge-pair interactions that can play a positive or negative role in the assembly of multi-subunit complexes such as the T cell receptor, or recruit signal transducing accessory mol...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687689609160579
更新日期:1996-04-01 00:00:00
abstract::Previous work from our laboratory has established bovine hippocampal membranes as a convenient natural source for studying neuronal receptors such as the G-protein coupled serotonin1A receptor. In this paper, we have explored the organization and dynamics of bovine hippocampal membranes using environment-sensitive and...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860600803223
更新日期:2006-09-01 00:00:00
abstract::The number of Signal Peptidase I (SPasel) molecules per E. coli cell was determined using western blot techniques. Different strains were found to contain approximately 1000 SPasel molecules per cell during exponential growth. Based on the activity of SPasel in vitro it could be estimated that this amount is sufficien...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689509072437
更新日期:1995-10-01 00:00:00
abstract::Dengue fever is one of the most widespread tropical diseases in the world. The disease is caused by a virus member of the Flaviviridae family, a group of enveloped positive sense single-stranded RNA viruses. Dengue virus infection is mediated by virus glycoprotein E, which binds to the cell surface. After uptake by en...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680701633091
更新日期:2008-02-01 00:00:00
abstract::Nanoparticles present a new collection of contrast agents for the field of in vivo molecular imaging. This review focuses on promising molecular imaging probes for optical and magnetic resonance imaging based on four representative nanomaterial(s) platforms: quantum dots, upconversion phosphors, superparamagnetic iron...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687688.2010.481640
更新日期:2010-10-01 00:00:00
abstract::The effect of cholesterol and lanosterol on the formation of structures in the gel/subgel phase of 1,2-dipalmitoylphosphatidylcholine was investigated using freeze-fracture electron microscopy and X-ray diffraction. Mixtures with up to 25 mol% sterol were analysed after annealing for between several days and some mont...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689709048181
更新日期:1997-10-01 00:00:00
abstract::The entry of substrates into, and the export of glururonides from, the lumen of hepatic endoplasmic reticulum (ER) in vitro (sealed microsomes) has been measured using radioactivity-labelled materials and a rapid filtration assay. Analysis of liver microsomes from a jaundiced patient showed the accumulation of bilirub...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689509072429
更新日期:1995-07-01 00:00:00
abstract::Membrane proteins are intrinsically involved in both human and pathogen physiology, and are the target of 60% of all marketed drugs. During the past decade, advances in the studies of membrane proteins using X-ray crystallography, electron microscopy and NMR-based techniques led to the elucidation of over 250 unique m...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2012.710341
更新日期:2013-03-01 00:00:00
abstract::In hair cells of the inner ear, phosphatidylserine (PS), detected with fluorescent annexin V labeling, was rapidly exposed on the external leaflet of apical plasma membranes upon dissection of the organ of Corti. PS externalization was unchanged by caspase inhibition, suggesting that externalization did not portend ap...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860600926883
更新日期:2007-01-01 00:00:00
abstract::Calpain, an intracellular calcium-dependent protease, is activated at cell membranes and cleaves cytoskeletal and submembranous proteins. Calpain is inferred to be a calcium-dependent regulator for cytoskeletal reorganization. Calpastatin, an endogenous calpain inhibitor, inhibits not only the proteolytic activity of ...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687689609160599
更新日期:1996-10-01 00:00:00
abstract::The cyanelles of the glaucocystophyte alga Cyanophora paradoxa resemble endosymbiotic cyanobacteria in morphology, pigmentation and, especially, in the presence of a peptidoglycan wall situated between the inner and outer envelope membranes. However, it is now clear that cyanelles in fact are primitive plastids. Phylo...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/09687860500041411
更新日期:2005-01-01 00:00:00
abstract::This investigation is aimed at defining the structural requirements for aliphatic polyamines to interact with mitochondrial binding sites, which are relevant for the regulation of the permeability transition and for mitochondrial polyamine uptake. The triamines N-aminoethyl-1,2-diaminoethane (dien) and N-aminopropyl-1...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680310001654916
更新日期:2004-03-01 00:00:00
abstract::The serotonin1A (5-HT1A) receptors are members of a superfamily of seven transmembrane domain receptors that couple to G-proteins. They appear to be involved in various behavioural and cognitive functions. This paper reports an efficient strategy to solubilize 5-HT1A receptors from bovine hippocampal membranes using t...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680210149586
更新日期:2002-07-01 00:00:00
abstract::Lipid rafts are specialized plasma membrane microdomains in which glycosphingolipids and cholesterol are major structural components. Their relative insolubility to nonionic detergents is the most widely used method to purify these structures. Several signalling proteins are associated with these microdomains in T lym...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860500063506
更新日期:2005-05-01 00:00:00
abstract::Membrane proteins are ubiquitous in biology and are key targets for therapeutic development. Despite this, our structural understanding has lagged behind that of their soluble counterparts. This review provides an overview of this important field, focusing in particular on the recent resurgence of electron microscopy ...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/09687688.2016.1221533
更新日期:2016-03-01 00:00:00
abstract::Time and distance scales in membranes are discussed in relation to the inference of domain structure from spectroscopic measurements. Each type of spectroscopy has a natural time scale set by the magnitude of the interactions that determine the spectral width. For fluid membranes, the lateral diffusion of the lipid mo...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687689509038489
更新日期:1995-01-01 00:00:00
abstract::Homotypic fusion between early endosomes can be reconstituted in vitro. By using wortmannin and LY294002, inhibitors of phosphatidylinositol (Pl) 3-kinase, a requirement for this activity has been established in order for fusion to proceed efficiently. It has been shown that Pl 3-kinase activity is required downstream...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/096876899294788
更新日期:1999-01-01 00:00:00