Abstract:
:The number of Signal Peptidase I (SPasel) molecules per E. coli cell was determined using western blot techniques. Different strains were found to contain approximately 1000 SPasel molecules per cell during exponential growth. Based on the activity of SPasel in vitro it could be estimated that this amount is sufficient to process all translocated precursors. SPasel did not appear to be under growth phase dependent control, but was constitutively expressed. The quantitative western blot technique was also used to establish the orientation and intactness of isolated inner membrane vesicles.
journal_name
Mol Membr Bioljournal_title
Molecular membrane biologyauthors
van Klompenburg W,Whitley P,Diemel R,von Heijne G,de Kruijff Bdoi
10.3109/09687689509072437subject
Has Abstractpub_date
1995-10-01 00:00:00pages
349-53issue
4eissn
0968-7688issn
1464-5203journal_volume
12pub_type
杂志文章abstract::ADP-ribosylation factor related protein 1 (ARFRP1) is a member of the ARF-family of GTPases which operate as molecular switches in the regulation of intracellular protein traffic. Deletion of the mouse Arfrp1 gene leads to embryonic lethality during early gastrulation, suggesting that ARFRP1 is required for cell adhes...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860600840100
更新日期:2006-11-01 00:00:00
abstract::Intracellular pH was measured with the pH-sensitive fluorescent probe BCECF in spinal cord neurones cultured from rat embryos. At an external pH of 7.3, the average steady-state pHi was 7.18 +/- 0.03 (SEM, n = 97) and 7.02 +/- 0.01 (n = 221) in HEPES-buffered and in bicarbonate-buffered medium, respectively. In both e...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680050117129
更新日期:2000-04-01 00:00:00
abstract::Membrane proteins are ubiquitous in biology and are key targets for therapeutic development. Despite this, our structural understanding has lagged behind that of their soluble counterparts. This review provides an overview of this important field, focusing in particular on the recent resurgence of electron microscopy ...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/09687688.2016.1221533
更新日期:2016-03-01 00:00:00
abstract::Absorption of dietary fat in the small intestine involves epithelial exposure to potentially harmful molecules such as bile salts and free fatty acids. We used organ culture of porcine jejunal explants incubated with a pre-digested mixture of fat (plant oil), bile and pancreatin to mimick the physiological process of ...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2013.780642
更新日期:2013-05-01 00:00:00
abstract::A group of prenyltransferases produce linear lipids by catalyzing consecutive condensation reactions of farnesyl diphosphate (FPP) with specific numbers of isopentenyl diphosphate (IPP), a common building block of isoprenoid compounds. Depending on the stereochemistry of the double bonds formed during IPP condensation...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687688.2012.674162
更新日期:2012-11-01 00:00:00
abstract::Neurotransmitter receptor trafficking in and out synapses has emerged as a key process to regulate synaptic transmission during synaptic development and plasticity both at excitatory and inhibitory synapses. Lateral diffusion of surface neurotransmitter receptors has recently emerged as a key pathway to regulate recep...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/09687680801958364
更新日期:2008-05-01 00:00:00
abstract::Equilibrative Nucleoside Transporters (SLC29) are a family of proteins that transport nucleosides, nucleobases and nucleoside analogue drugs across cellular membranes. ENT1 is expressed ubiquitously in mammalian tissues and responsible for a significant portion of nucleoside analog drug uptake in humans. Despite the i...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2011.604861
更新日期:2011-09-01 00:00:00
abstract::The eukaryotic endomembrane system (ES) is served by hundreds of dedicated proteins. Experimental characterization of the ES-associated molecular machinery in several model eukaryotes complemented by a recent progress in phylogenomics and comparative genomics have revealed a conserved complex core of the machinery tha...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687688.2010.521201
更新日期:2010-11-01 00:00:00
abstract::Insolubility of membrane components in non-ionic detergents such as Triton X-100 at low temperature is a widely used biochemical criterion to identify, isolate and characterize membrane domains. In this work, we monitored the detergent insolubility of the serotonin(1A) receptor in CHO cell membranes and its modulation...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2012.688147
更新日期:2012-11-01 00:00:00
abstract::Site-directed mutagenesis and site-directed fluorescence spectroscopy demonstrate that Cys148 interacts hydrophobically with the galactosyl moiety of substrates of the lactose permease of Escherichia coli. By taking advantage of the finding that labelling of single-Cys148 permease with the thiol-specific fluorophore 2...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689809027513
更新日期:1998-01-01 00:00:00
abstract::Neurotransmitter transporters are regulated through a variety of signal transduction mechanisms which appear to operate in order to maintain appropriate levels of transmitter in the synaptic cleft. One such mechanism is the trafficking of the transporter in association with synaptic vesicle release machinery. This rep...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Flavonoids are ubiquitous polyphenolic compounds, found in vascular plants, which are endowed with a large variety of biological effects. Some of these effects have been assumed to result from interactions with the cell plasma membrane. In order to investigate the nature of these interactions a fluorescence study was ...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/096876899294616
更新日期:1999-04-01 00:00:00
abstract::Significant progress has been made in membrane protein engineering over the last 5 years, based largely on the re-design of existing scaffolds. Engineering techniques that have been employed include direct genetic engineering, both covalent and non-covalent modification, unnatural amino acid mutagenesis and total synt...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/09687680410001716853
更新日期:2004-07-01 00:00:00
abstract::The number of structures of integral membrane proteins from higher eukaryotes is steadily increasing due to a number of innovative protein engineering and crystallization strategies devised over the last few years. However, it is sobering to reflect that these structures represent only a tiny proportion of the total n...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687688.2012.703703
更新日期:2013-02-01 00:00:00
abstract::Notch is a vitally important signalling receptor controlling cell fate determination and pattern formation in numerous ways during development of both invertebrate and vertebrate species. An intriguing pathway for the Notch signal has emerged where, after ligand-dependent proteolysis, an intracellular fragment of the ...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/09687680110112929
更新日期:2002-01-01 00:00:00
abstract::Caveolae are approximately 50-100 nm membrane micro-invaginations associated with the plasma membrane of a wide variety of cells. Although they were first identified in transmission electron micrographs approximately 40 years ago, their exact function(s) has remained controversial. Two well-established functions inclu...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687689509038506
更新日期:1995-01-01 00:00:00
abstract::The Amt/Mep/Rh family of integral membrane proteins comprises ammonium transporters of bacteria, archaea and eukarya, as well as the Rhesus proteins found in animals. They play a central role in the uptake of reduced nitrogen for biosynthetic purposes, in energy metabolism, or in renal excretion. Recent structural inf...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/09687680701388423
更新日期:2007-09-01 00:00:00
abstract::Glu-269, which is located on the hydrophilic face of putative helix VIII in the lactose permease of Escherichia coli, has been replaced with Asp, Gln or Cys by oligonucleotide-directed, site specific mutagenesis. Cells expressing Asp-269 permease exhibit no lactose accumulation or lactose-induced H+ translocation, but...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689409161024
更新日期:1994-01-01 00:00:00
abstract::Small GTPase Rab36 is homologous to Rab34 with 56% amino acid sequence identity. Rab34 was characterized as a Golgi-associated Rab protein and regulates lysosomal positioning through interaction with RILP; however, the properties and functions of Rab36 have not been investigated. To investigate Rab36, we constructed E...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687680903417470
更新日期:2010-01-01 00:00:00
abstract::At least four different types of interaction between protein transmembrane helices have been described to date. These include the use of charge-pair interactions that can play a positive or negative role in the assembly of multi-subunit complexes such as the T cell receptor, or recruit signal transducing accessory mol...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687689609160579
更新日期:1996-04-01 00:00:00
abstract::The entry of substrates into, and the export of glururonides from, the lumen of hepatic endoplasmic reticulum (ER) in vitro (sealed microsomes) has been measured using radioactivity-labelled materials and a rapid filtration assay. Analysis of liver microsomes from a jaundiced patient showed the accumulation of bilirub...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689509072429
更新日期:1995-07-01 00:00:00
abstract::The transverse distribution of phosphatidylglycerol (PG) across the inner membrane of Escherichia coli was studied. The oxidation of PG by periodate was used to discriminate between the PG present in the cytoplasmic and in the periplasmic leaflet of the inner membrane. Applied to large unilamellar vesicles derived of ...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689709048168
更新日期:1997-01-01 00:00:00
abstract::Glucosyltransferases (Gtrs) and O-acetyltransferase (Oac) are integral membrane proteins embedded within the cytoplasmic membrane of Shigella flexneri. Gtrs and Oac are responsible for unidirectional host serotype conversion by altering the epitopic properties of the bacterial surface lipopolysaccharide (LPS) O-antige...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687680903552250
更新日期:2010-04-01 00:00:00
abstract::A lactate permease was biochemically identified in Candida albicans RM1000 presenting the following kinetic parameters at pH 5.0: Km 0.33+/-0.09 mM and Vmax 0.85+/-0.06 nmol s(-1) mg dry wt(-1). Lactate uptake was competitively inhibited by pyruvic and propionic acids; acetic acid behaved as a non-competitive substrat...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860400011373
更新日期:2004-11-01 00:00:00
abstract::Gap junctions are plasma membrane spatial microdomains constructed of assemblies of channel proteins called connexins in vertebrates and innexins in invertebrates. The channels provide direct intercellular communication pathways allowing rapid exchange of ions and metabolites up to approximately 1 kD in size. Approxim...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/09687680210139839
更新日期:2002-04-01 00:00:00
abstract::Membrane proteins are intrinsically involved in both human and pathogen physiology, and are the target of 60% of all marketed drugs. During the past decade, advances in the studies of membrane proteins using X-ray crystallography, electron microscopy and NMR-based techniques led to the elucidation of over 250 unique m...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2012.710341
更新日期:2013-03-01 00:00:00
abstract::A structural characterization of a synthetic peptide corresponding to the fourth transmembrane domain (M4-TMD) of the gamma-subunit of the nicotinic acetylcholine receptor from Torpedo californica has been undertaken. Solid-state NMR and CD spectroscopy studies indicate that upon reconstitution into lipid vesicles or ...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860500370653
更新日期:2005-11-01 00:00:00
abstract::TGN38 is a heavily glycosylated, type I integral membrane protein which is predominantly localized to the trans Golgi network (TGN), but which constitutively traffics between the TGN and the cell surface. The trafficking of TGN38 has been extensively studied in non-polarized cells, and a short, tyrosine-based, peptide...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689809074524
更新日期:1998-07-01 00:00:00
abstract::Radiation-based therapies aided by nanoparticles have been developed for decades, and can be primarily categorized into two main platforms. First, delivery of payload of photo-reactive drugs (photosensitizers) using the conventional nanoparticles, and second, design and development of photo-triggerable nanoparticles (...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687688.2010.507788
更新日期:2010-10-01 00:00:00
abstract::Eleven sequenced anion exchanger (AE; band 3) proteins, including five AE1, four AE2 and two AE3 proteins, comprise the anion exchanger family (AEF) of homologous proteins. Eliminating the rat and rabbit proteins that are nearly identical to the corresponding mouse proteins, seven dissimilar members of this family wer...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689509027507
更新日期:1995-04-01 00:00:00