TGN38 cycles via the basolateral membrane of polarized Caco-2 cells.

abstract：:In Saccharomyces cerevisiae Jen1p is a lactate/proton symporter belonging to the lactate/pyruvate:H(+) symporter subfamily (TC#2.A.1.12.2) of the Major Facilitator Superfamily. We investigated structure-function relationships of Jen1p using a rational mutational analysis based on the identification of conserved amino ...

journal_title：Molecular membrane biology

authors： Soares-Silva I,Paiva S,Diallinas G,Casal M

更新日期：2007-09-01 00:00:00

abstract：:Neurotransmitter transporters are regulated through a variety of signal transduction mechanisms which appear to operate in order to maintain appropriate levels of transmitter in the synaptic cleft. One such mechanism is the trafficking of the transporter in association with synaptic vesicle release machinery. This rep...

journal_title：Molecular membrane biology

authors： Horton N,Quick MW

更新日期：2001-01-01 00:00:00

abstract：:Membrane proteins are ubiquitous in biology and are key targets for therapeutic development. Despite this, our structural understanding has lagged behind that of their soluble counterparts. This review provides an overview of this important field, focusing in particular on the recent resurgence of electron microscopy ...

journal_title：Molecular membrane biology

authors： Rawson S,Davies S,Lippiat JD,Muench SP

更新日期：2016-03-01 00:00:00

abstract：:Amiodarone (AMI) is a low water-solubility drug, which is very useful in the treatment of severe cardiac disease. Its adverse effects are associated with toxicity in different tissues. Several antioxidants have been shown to reduce, and prevent AMI toxicity. The aim of this work was to develop and characterize Dimyris...

journal_title：Molecular membrane biology

authors： Benedini L,Antollini S,Fanani ML,Palma S,Messina P,Schulz P

更新日期：2014-03-01 00:00:00

abstract：:Neurotransmitter receptor trafficking in and out synapses has emerged as a key process to regulate synaptic transmission during synaptic development and plasticity both at excitatory and inhibitory synapses. Lateral diffusion of surface neurotransmitter receptors has recently emerged as a key pathway to regulate recep...

journal_title：Molecular membrane biology

authors： Groc L,Choquet D

更新日期：2008-05-01 00:00:00

abstract：:Eleven sequenced anion exchanger (AE; band 3) proteins, including five AE1, four AE2 and two AE3 proteins, comprise the anion exchanger family (AEF) of homologous proteins. Eliminating the rat and rabbit proteins that are nearly identical to the corresponding mouse proteins, seven dissimilar members of this family wer...

journal_title：Molecular membrane biology

authors： Espanol MJ,Saier MH Jr

更新日期：1995-04-01 00:00:00

abstract：:Palmitoylation is required for the activities of several cancer-associated proteins, making the palmitoyl acyltransferase (PAT) enzymes that catalyze these reactions potential targets for anticancer therapeutics. In this study, we sought to identify and characterize a human PAT with activity toward N-terminally myrist...

journal_title：Molecular membrane biology

authors： Draper JM,Smith CD

更新日期：2010-04-01 00:00:00

abstract：:The Signal Recognition Particle (SRP) plays a critical role in the sorting of nascent secretory and membrane proteins. Remarkably, this function has been conserved from bacteria, where SRP delivers proteins to the inner membrane, through to eukaryotes, where SRP is required for targeting of proteins to the endoplasmic...

journal_title：Molecular membrane biology

authors： Pool MR

更新日期：2005-01-01 00:00:00

abstract：:The entry of substrates into, and the export of glururonides from, the lumen of hepatic endoplasmic reticulum (ER) in vitro (sealed microsomes) has been measured using radioactivity-labelled materials and a rapid filtration assay. Analysis of liver microsomes from a jaundiced patient showed the accumulation of bilirub...

journal_title：Molecular membrane biology

authors： Waddell ID,Robertson K,Burchell A,Hume R,Burchell B

更新日期：1995-07-01 00:00:00

abstract：:Ionotropic glutamate receptors belong to the superfamily of P-loop channels as well as K(+), Na(+), and Ca(2+) channels. However, the structural similarity between ion channels of the glutamate receptors and K(+) channels is a matter of discussion. The aim of this study was to analyze differences between the structure...

journal_title：Molecular membrane biology

authors： Tikhonov DB

更新日期：2007-03-01 00:00:00

abstract：:A lactate permease was biochemically identified in Candida albicans RM1000 presenting the following kinetic parameters at pH 5.0: Km 0.33+/-0.09 mM and Vmax 0.85+/-0.06 nmol s(-1) mg dry wt(-1). Lactate uptake was competitively inhibited by pyruvic and propionic acids; acetic acid behaved as a non-competitive substrat...

journal_title：Molecular membrane biology

authors： Soares-Silva I,Paiva S,Kötter P,Entian KD,Casal M

更新日期：2004-11-01 00:00:00

abstract：:We have previously shown that a mixture of cerebrosides obtained from dried tubers of herb Typhonium giganteum Engl. plays a neuroprotective role in the ischemic brain through its effect on activation of BK(Ca) channels. It is very curious to know whether a single pure cerebroside compound could activate the BK(Ca) ch...

journal_title：Molecular membrane biology

authors： Xu H,Qi J,Wang G,Deng H,Qi Z

更新日期：2011-02-01 00:00:00

abstract：:Equilibrative Nucleoside Transporters (SLC29) are a family of proteins that transport nucleosides, nucleobases and nucleoside analogue drugs across cellular membranes. ENT1 is expressed ubiquitously in mammalian tissues and responsible for a significant portion of nucleoside analog drug uptake in humans. Despite the i...

journal_title：Molecular membrane biology

authors： Reyes G,Nivillac NM,Karim MZ,Desouza L,Siu KW,Coe IR

更新日期：2011-09-01 00:00:00

abstract：:In hair cells of the inner ear, phosphatidylserine (PS), detected with fluorescent annexin V labeling, was rapidly exposed on the external leaflet of apical plasma membranes upon dissection of the organ of Corti. PS externalization was unchanged by caspase inhibition, suggesting that externalization did not portend ap...

journal_title：Molecular membrane biology

authors： Shi X,Gillespie PG,Nuttall AL

更新日期：2007-01-01 00:00:00

abstract：:Glycosyl-phosphatidylinositol (GPI)-anchored proteins are enriched in cholesterol- and sphingolipid-rich lipid rafts within the membrane. Rafts are known to have roles in cellular organization and function, but little is understood about the factors controlling the distribution of proteins in rafts. We have used atomi...

journal_title：Molecular membrane biology

authors： Garner AE,Smith DA,Hooper NM

更新日期：2007-05-01 00:00:00

abstract：:The transporter SbtA is a high affinity Na+-dependent HCO3- uptake system present in a majority of cyanobacterial clades. It functions in conjunction with CO2 uptake systems and other HCO3- uptake systems to allow cyanobacteria to accumulate high levels of HCO3- used to support efficient photosynthetic CO2 fixation vi...

journal_title：Molecular membrane biology

authors： Price GD,Shelden MC,Howitt SM

更新日期：2011-08-01 00:00:00

abstract：:Intracellular pH was measured with the pH-sensitive fluorescent probe BCECF in spinal cord neurones cultured from rat embryos. At an external pH of 7.3, the average steady-state pHi was 7.18 +/- 0.03 (SEM, n = 97) and 7.02 +/- 0.01 (n = 221) in HEPES-buffered and in bicarbonate-buffered medium, respectively. In both e...

journal_title：Molecular membrane biology

authors： Brechenmacher C,Rodeau JL

更新日期：2000-04-01 00:00:00

abstract：:2B is a 99 amino acid membrane protein encoded by enteroviruses such as polio and coxsackie viruses with two transmembrane domains. The protein is found to make membranes of infected cells permeable. Using a computational approach which positions the models and assesses stability by molecular dynamics (MD) simulations...

journal_title：Molecular membrane biology

authors： Patargias G,Barke T,Watts A,Fischer WB

更新日期：2009-08-01 00:00:00

abstract：:A group of prenyltransferases produce linear lipids by catalyzing consecutive condensation reactions of farnesyl diphosphate (FPP) with specific numbers of isopentenyl diphosphate (IPP), a common building block of isoprenoid compounds. Depending on the stereochemistry of the double bonds formed during IPP condensation...

journal_title：Molecular membrane biology

authors： Teng KH,Liang PH

更新日期：2012-11-01 00:00:00

abstract：:This investigation is aimed at defining the structural requirements for aliphatic polyamines to interact with mitochondrial binding sites, which are relevant for the regulation of the permeability transition and for mitochondrial polyamine uptake. The triamines N-aminoethyl-1,2-diaminoethane (dien) and N-aminopropyl-1...

journal_title：Molecular membrane biology

authors： Dalla Via L,Salvi M,Di Noto V,Stefanelli C,Toninello A

更新日期：2004-03-01 00:00:00

abstract：:Numerous cell surface receptors propagate activation signals to the interior of the cell via tyrosine phosphorylation of transmembrane proteins. This leads to the phosphotyrosine (PiY)-mediated recruitment of cytoplasmic signalling protein complexes which catalyze crucial biochemical signalling reactions. Here we desc...

journal_title：Molecular membrane biology

authors： Sangani D,Venien-Bryan C,Harder T

更新日期：2009-04-01 00:00:00

abstract：:PagP is a bacterial outer membrane protein consisting of an 8 stranded transmembrane beta-barrel and an N-terminal alpha-helix. It is an enzyme which catalyses transfer of a palmitoyl chain from a phospholipid to lipid A. Molecular dynamics simulations have been used to compare the dynamic behaviour in simulations sta...

journal_title：Molecular membrane biology

authors： Cox K,Sansom MS

更新日期：2009-05-01 00:00:00

abstract：:Solid-state NMR combined with sample deuteration was used to probe the proximity of the low-affinity substrate D-glucose to its binding site within the Escherichia coli sugar transport protein GalP. Samples of E. coli inner membranes with amplified expression of GalP were incubated in D(2)O with D-[(13)C(6)]glucose an...

journal_title：Molecular membrane biology

authors： Patching SG,Henderson PJ,Sharples DJ,Middleton DA

更新日期：2013-03-01 00:00:00

abstract：:4-Hydroxy-2-nonenal (4-HNE) is a reactive aldehyde and a lipid peroxidation product formed in biological tissues under physiological and pathological conditions. Its concentration increases with oxidative stress and induces deleterious modifications of proteins and membranes. Mitochondrial and cytosolic isoforms of cr...

journal_title：Molecular membrane biology

authors： Maniti O,François-Moutal L,Lecompte MF,Vial C,Lagarde M,Guichardant M,Marcillat O,Granjon T

更新日期：2015-01-01 00:00:00

abstract：:Small GTPase Rab36 is homologous to Rab34 with 56% amino acid sequence identity. Rab34 was characterized as a Golgi-associated Rab protein and regulates lysosomal positioning through interaction with RILP; however, the properties and functions of Rab36 have not been investigated. To investigate Rab36, we constructed E...

journal_title：Molecular membrane biology

authors： Chen L,Hu J,Yun Y,Wang T

更新日期：2010-01-01 00:00:00

abstract：:The mitochondrial ADP/ATP carrier imports ADP from the cytosol into the mitochondrial matrix for its conversion to ATP by ATP synthase and exports ATP out of the mitochondrion to replenish the eukaryotic cell with chemical energy. Here the substrate specificity of the human mitochondrial ADP/ATP carrier AAC1 was deter...

journal_title：Molecular membrane biology

authors： Mifsud J,Ravaud S,Krammer EM,Chipot C,Kunji ER,Pebay-Peyroula E,Dehez F

更新日期：2013-03-01 00:00:00

abstract：:Hereditary spherocytosis (HS), a common human inherited haemolytic anaemia, is associated with partial deficiency of different erythrocyte membrane proteins. In a subset of dominant HS, a partial membrane expression deficiency of band 3, the erythrocyte anion exchanger (AE1), have previously been characterized, and se...

journal_title：Molecular membrane biology

authors： Dhermy D,Burnier O,Bourgeois M,Grandchamp B

更新日期：1999-10-01 00:00:00

abstract：:Caveolae are approximately 50-100 nm membrane micro-invaginations associated with the plasma membrane of a wide variety of cells. Although they were first identified in transmission electron micrographs approximately 40 years ago, their exact function(s) has remained controversial. Two well-established functions inclu...

journal_title：Molecular membrane biology

authors： Lisanti MP,Tang Z,Scherer PE,Kübler E,Koleske AJ,Sargiacomo M

更新日期：1995-01-01 00:00:00

abstract：:The assessment of the transverse distribution and mobility of NBD-labelled phospholipid analogues in biological membranes by selective chemical destruction of fluorescent label in the outer monolayer with dithionite has been investigated using resealed erythrocyte ghosts as a model system. The distribution of those an...

journal_title：Molecular membrane biology

authors： Pomorski T,Herrmann A,Zachowski A,Devaux PF,Müller P

更新日期：1994-01-01 00:00:00

abstract：:Homotypic fusion between early endosomes can be reconstituted in vitro. By using wortmannin and LY294002, inhibitors of phosphatidylinositol (Pl) 3-kinase, a requirement for this activity has been established in order for fusion to proceed efficiently. It has been shown that Pl 3-kinase activity is required downstream...

journal_title：Molecular membrane biology

authors： Mills IG,Jones AT,Clague MJ

更新日期：1999-01-01 00:00:00