Functional engineered channels and pores (Review).

abstract：:The serotonin1A (5-HT1A) receptors are members of a superfamily of seven transmembrane domain receptors that couple to G-proteins. They appear to be involved in various behavioural and cognitive functions. This paper reports an efficient strategy to solubilize 5-HT1A receptors from bovine hippocampal membranes using t...

journal_title：Molecular membrane biology

authors： Chattopadhyay A,Harikumar KG,Kalipatnapu S

更新日期：2002-07-01 00:00:00

abstract：:Time and distance scales in membranes are discussed in relation to the inference of domain structure from spectroscopic measurements. Each type of spectroscopy has a natural time scale set by the magnitude of the interactions that determine the spectral width. For fluid membranes, the lateral diffusion of the lipid mo...

journal_title：Molecular membrane biology

authors： Bloom M,Thewalt JL

更新日期：1995-01-01 00:00:00

abstract：:The cyanelles of the glaucocystophyte alga Cyanophora paradoxa resemble endosymbiotic cyanobacteria in morphology, pigmentation and, especially, in the presence of a peptidoglycan wall situated between the inner and outer envelope membranes. However, it is now clear that cyanelles in fact are primitive plastids. Phylo...

journal_title：Molecular membrane biology

authors： Steiner JM,Löffelhardt W

更新日期：2005-01-01 00:00:00

abstract：:Notch is a vitally important signalling receptor controlling cell fate determination and pattern formation in numerous ways during development of both invertebrate and vertebrate species. An intriguing pathway for the Notch signal has emerged where, after ligand-dependent proteolysis, an intracellular fragment of the ...

journal_title：Molecular membrane biology

authors： Baron M,Aslam H,Flasza M,Fostier M,Higgs JE,Mazaleyrat SL,Wilkin MB

更新日期：2002-01-01 00:00:00

abstract：:Spontaneous membrane adsorption, folding and insertion of the synthetic WALP16 and KALP16 peptides was studied by computer simulations starting from completely extended conformations. The peptides were simulated using an unmodified all-atom force field in combination with an efficient Monte Carlo sampling algorithm. T...

journal_title：Molecular membrane biology

authors： Ulmschneider MB,Ulmschneider JP

更新日期：2008-04-01 00:00:00

abstract：:Hereditary spherocytosis (HS), a common human inherited haemolytic anaemia, is associated with partial deficiency of different erythrocyte membrane proteins. In a subset of dominant HS, a partial membrane expression deficiency of band 3, the erythrocyte anion exchanger (AE1), have previously been characterized, and se...

journal_title：Molecular membrane biology

authors： Dhermy D,Burnier O,Bourgeois M,Grandchamp B

更新日期：1999-10-01 00:00:00

abstract：:The eukaryotic endomembrane system (ES) is served by hundreds of dedicated proteins. Experimental characterization of the ES-associated molecular machinery in several model eukaryotes complemented by a recent progress in phylogenomics and comparative genomics have revealed a conserved complex core of the machinery tha...

journal_title：Molecular membrane biology

authors： Elias M

更新日期：2010-11-01 00:00:00

abstract：:Membrane proteins are ubiquitous in biology and are key targets for therapeutic development. Despite this, our structural understanding has lagged behind that of their soluble counterparts. This review provides an overview of this important field, focusing in particular on the recent resurgence of electron microscopy ...

journal_title：Molecular membrane biology

authors： Rawson S,Davies S,Lippiat JD,Muench SP

更新日期：2016-03-01 00:00:00

abstract：:Glu-269, which is located on the hydrophilic face of putative helix VIII in the lactose permease of Escherichia coli, has been replaced with Asp, Gln or Cys by oligonucleotide-directed, site specific mutagenesis. Cells expressing Asp-269 permease exhibit no lactose accumulation or lactose-induced H+ translocation, but...

journal_title：Molecular membrane biology

authors： Ujwal ML,Sahin-Tóth M,Persson B,Kaback HR

更新日期：1994-01-01 00:00:00

abstract：:4-Hydroxy-2-nonenal (4-HNE) is a reactive aldehyde and a lipid peroxidation product formed in biological tissues under physiological and pathological conditions. Its concentration increases with oxidative stress and induces deleterious modifications of proteins and membranes. Mitochondrial and cytosolic isoforms of cr...

journal_title：Molecular membrane biology

authors： Maniti O,François-Moutal L,Lecompte MF,Vial C,Lagarde M,Guichardant M,Marcillat O,Granjon T

更新日期：2015-01-01 00:00:00

abstract：:Microorganisms that survive in natural environments form organized multicellular communities, biofilms and colonies with specific properties. During stress and nutrient limitation, slow growing and senescent cells in such communities retain vital processes by maintaining plasma membrane integrity and retaining the abi...

journal_title：Molecular membrane biology

authors： Palková Z,Váchová L,Gásková D,Kucerová H

更新日期：2009-05-01 00:00:00

abstract：:The present review introduces the concept of 'non-permanent membrane proteins', to encompass the wide variety of proteins that are not found in a stable membrane-bound form under physiological conditions, yet they interact with the membrane at some stage of their specific course of action. Non-permanent membrane prote...

journal_title：Molecular membrane biology

authors： Goñi FM

更新日期：2002-10-01 00:00:00

abstract：:Neurotransmitter receptor trafficking in and out synapses has emerged as a key process to regulate synaptic transmission during synaptic development and plasticity both at excitatory and inhibitory synapses. Lateral diffusion of surface neurotransmitter receptors has recently emerged as a key pathway to regulate recep...

journal_title：Molecular membrane biology

authors： Groc L,Choquet D

更新日期：2008-05-01 00:00:00

abstract：:2B is a 99 amino acid membrane protein encoded by enteroviruses such as polio and coxsackie viruses with two transmembrane domains. The protein is found to make membranes of infected cells permeable. Using a computational approach which positions the models and assesses stability by molecular dynamics (MD) simulations...

journal_title：Molecular membrane biology

authors： Patargias G,Barke T,Watts A,Fischer WB

更新日期：2009-08-01 00:00:00

abstract：:In Saccharomyces cerevisiae Jen1p is a lactate/proton symporter belonging to the lactate/pyruvate:H(+) symporter subfamily (TC#2.A.1.12.2) of the Major Facilitator Superfamily. We investigated structure-function relationships of Jen1p using a rational mutational analysis based on the identification of conserved amino ...

journal_title：Molecular membrane biology

authors： Soares-Silva I,Paiva S,Diallinas G,Casal M

更新日期：2007-09-01 00:00:00

abstract：:A lactate permease was biochemically identified in Candida albicans RM1000 presenting the following kinetic parameters at pH 5.0: Km 0.33+/-0.09 mM and Vmax 0.85+/-0.06 nmol s(-1) mg dry wt(-1). Lactate uptake was competitively inhibited by pyruvic and propionic acids; acetic acid behaved as a non-competitive substrat...

journal_title：Molecular membrane biology

authors： Soares-Silva I,Paiva S,Kötter P,Entian KD,Casal M

更新日期：2004-11-01 00:00:00

abstract：:Notch receptor signalling plays a central role in development and its misfunction has been linked to a number of diseases. In the cannonical Notch signalling pathway, ligand binding to Notch activates a series of proteolytic cleavages that release the Notch intracellular domain for trafficking to the nucleus, where it...

journal_title：Molecular membrane biology

authors： Wilkin MB,Baron M

更新日期：2005-07-01 00:00:00

abstract：:The Amt/Mep/Rh family of integral membrane proteins comprises ammonium transporters of bacteria, archaea and eukarya, as well as the Rhesus proteins found in animals. They play a central role in the uptake of reduced nitrogen for biosynthetic purposes, in energy metabolism, or in renal excretion. Recent structural inf...

journal_title：Molecular membrane biology

authors： Andrade SL,Einsle O

更新日期：2007-09-01 00:00:00

abstract：:Previous work from our laboratory has established bovine hippocampal membranes as a convenient natural source for studying neuronal receptors such as the G-protein coupled serotonin1A receptor. In this paper, we have explored the organization and dynamics of bovine hippocampal membranes using environment-sensitive and...

journal_title：Molecular membrane biology

authors： Mukherjee S,Kalipatnapu S,Pucadyil TJ,Chattopadhyay A

更新日期：2006-09-01 00:00:00

abstract：:Recent advances have revealed much about the signaling events and molecular components associated with autophagy. Although it is clear that there are multiple points of intersection and connection between autophagy and known vesicular membrane transport processes between membrane compartments, autophagy is modulated b...

journal_title：Molecular membrane biology

authors： Chan SN,Tang BL

更新日期：2013-12-01 00:00:00

abstract：:Palmitoylation is required for the activities of several cancer-associated proteins, making the palmitoyl acyltransferase (PAT) enzymes that catalyze these reactions potential targets for anticancer therapeutics. In this study, we sought to identify and characterize a human PAT with activity toward N-terminally myrist...

journal_title：Molecular membrane biology

authors： Draper JM,Smith CD

更新日期：2010-04-01 00:00:00

abstract：:Vascular-targeted drug delivery systems could provide more efficient and effective pharmaceutical interventions for treating a variety of diseases including cardiovascular, pulmonary, inflammatory, and malignant disorders. However, several factors must be taken into account when designing these systems. The diverse bl...

journal_title：Molecular membrane biology

authors： Huang RB,Mocherla S,Heslinga MJ,Charoenphol P,Eniola-Adefeso O

更新日期：2010-08-01 00:00:00

abstract：:Intracellular pH was measured with the pH-sensitive fluorescent probe BCECF in spinal cord neurones cultured from rat embryos. At an external pH of 7.3, the average steady-state pHi was 7.18 +/- 0.03 (SEM, n = 97) and 7.02 +/- 0.01 (n = 221) in HEPES-buffered and in bicarbonate-buffered medium, respectively. In both e...

journal_title：Molecular membrane biology

authors： Brechenmacher C,Rodeau JL

更新日期：2000-04-01 00:00:00

abstract：:Absorption of dietary fat in the small intestine involves epithelial exposure to potentially harmful molecules such as bile salts and free fatty acids. We used organ culture of porcine jejunal explants incubated with a pre-digested mixture of fat (plant oil), bile and pancreatin to mimick the physiological process of ...

journal_title：Molecular membrane biology

authors： Danielsen EM,Hansen GH,Rasmussen K,Niels-Christiansen LL

更新日期：2013-05-01 00:00:00

abstract：:Dengue fever is one of the most widespread tropical diseases in the world. The disease is caused by a virus member of the Flaviviridae family, a group of enveloped positive sense single-stranded RNA viruses. Dengue virus infection is mediated by virus glycoprotein E, which binds to the cell surface. After uptake by en...

journal_title：Molecular membrane biology

authors： Stauffer F,Melo MN,Carneiro FA,Sousa FJ,Juliano MA,Juliano L,Mohana-Borges R,Da Poian AT,Castanho MA

更新日期：2008-02-01 00:00:00

abstract：:Membrane microdomains enriched in cholesterol, sphingolipids (rafts), and specific proteins are involved in important physiological functions. However their structure, size and stability are still controversial. Given that detergent-resistant membranes (DRMs) are in the liquid-ordered state and are rich in raft-like c...

journal_title：Molecular membrane biology

authors： Casadei BR,De Oliveira Carvalho P,Riske KA,Barbosa Rde M,De Paula E,Domingues CC

更新日期：2014-09-01 00:00:00

abstract：:This investigation is aimed at defining the structural requirements for aliphatic polyamines to interact with mitochondrial binding sites, which are relevant for the regulation of the permeability transition and for mitochondrial polyamine uptake. The triamines N-aminoethyl-1,2-diaminoethane (dien) and N-aminopropyl-1...

journal_title：Molecular membrane biology

authors： Dalla Via L,Salvi M,Di Noto V,Stefanelli C,Toninello A

更新日期：2004-03-01 00:00:00

abstract：:Homotypic fusion between early endosomes can be reconstituted in vitro. By using wortmannin and LY294002, inhibitors of phosphatidylinositol (Pl) 3-kinase, a requirement for this activity has been established in order for fusion to proceed efficiently. It has been shown that Pl 3-kinase activity is required downstream...

journal_title：Molecular membrane biology

authors： Mills IG,Jones AT,Clague MJ

更新日期：1999-01-01 00:00:00

abstract：:Constitutive secretion is used to deliver newly synthesized proteins to the cell surface and to the extracellular milieu. The trans-Golgi network is a key station along this route that mediates sorting of proteins into distinct transport pathways, aided in part by clathrin and adaptor proteins. Subsequent movement of ...

journal_title：Molecular membrane biology

authors： Ponnambalam S,Baldwin SA

更新日期：2003-04-01 00:00:00

abstract：:CitS of Klebsiella pneumoniae and GltS of Escherichia coli are Na+-dependent secondary transporters from different families that are believed to share the same fold and quaternary structure. A 10 kDa protein tag (Biotin Acceptor Domain [BAD]) was fused to the N-terminus of both proteins (CitS-BAD1 and GltS-BAD1, respe...

journal_title：Molecular membrane biology

authors： Krupnik T,Dobrowolski A,Lolkema JS

更新日期：2011-08-01 00:00:00