Overexpression of membrane proteins in mammalian cells for structural studies.

abstract：:Caveolae are approximately 50-100 nm membrane micro-invaginations associated with the plasma membrane of a wide variety of cells. Although they were first identified in transmission electron micrographs approximately 40 years ago, their exact function(s) has remained controversial. Two well-established functions inclu...

journal_title：Molecular membrane biology

authors： Lisanti MP,Tang Z,Scherer PE,Kübler E,Koleske AJ,Sargiacomo M

更新日期：1995-01-01 00:00:00

abstract：:CitS of Klebsiella pneumoniae and GltS of Escherichia coli are Na+-dependent secondary transporters from different families that are believed to share the same fold and quaternary structure. A 10 kDa protein tag (Biotin Acceptor Domain [BAD]) was fused to the N-terminus of both proteins (CitS-BAD1 and GltS-BAD1, respe...

journal_title：Molecular membrane biology

authors： Krupnik T,Dobrowolski A,Lolkema JS

更新日期：2011-08-01 00:00:00

abstract：:The eukaryotic endomembrane system (ES) is served by hundreds of dedicated proteins. Experimental characterization of the ES-associated molecular machinery in several model eukaryotes complemented by a recent progress in phylogenomics and comparative genomics have revealed a conserved complex core of the machinery tha...

journal_title：Molecular membrane biology

authors： Elias M

更新日期：2010-11-01 00:00:00

abstract：:Insolubility of membrane components in non-ionic detergents such as Triton X-100 at low temperature is a widely used biochemical criterion to identify, isolate and characterize membrane domains. In this work, we monitored the detergent insolubility of the serotonin(1A) receptor in CHO cell membranes and its modulation...

journal_title：Molecular membrane biology

authors： Sahu SK,Saxena R,Chattopadhyay A

更新日期：2012-11-01 00:00:00

abstract：:Homotypic fusion between early endosomes can be reconstituted in vitro. By using wortmannin and LY294002, inhibitors of phosphatidylinositol (Pl) 3-kinase, a requirement for this activity has been established in order for fusion to proceed efficiently. It has been shown that Pl 3-kinase activity is required downstream...

journal_title：Molecular membrane biology

authors： Mills IG,Jones AT,Clague MJ

更新日期：1999-01-01 00:00:00

abstract：:Lipid rafts are specialized plasma membrane microdomains in which glycosphingolipids and cholesterol are major structural components. Their relative insolubility to nonionic detergents is the most widely used method to purify these structures. Several signalling proteins are associated with these microdomains in T lym...

journal_title：Molecular membrane biology

authors： Salgado FJ,Piñeiro A,Canda-Sánchez A,Lojo J,Nogueira M

更新日期：2005-05-01 00:00:00

abstract：:Microorganisms that survive in natural environments form organized multicellular communities, biofilms and colonies with specific properties. During stress and nutrient limitation, slow growing and senescent cells in such communities retain vital processes by maintaining plasma membrane integrity and retaining the abi...

journal_title：Molecular membrane biology

authors： Palková Z,Váchová L,Gásková D,Kucerová H

更新日期：2009-05-01 00:00:00

abstract：:Nanoparticles present a new collection of contrast agents for the field of in vivo molecular imaging. This review focuses on promising molecular imaging probes for optical and magnetic resonance imaging based on four representative nanomaterial(s) platforms: quantum dots, upconversion phosphors, superparamagnetic iron...

journal_title：Molecular membrane biology

authors： Rosenblum LT,Kosaka N,Mitsunaga M,Choyke PL,Kobayashi H

更新日期：2010-10-01 00:00:00

abstract：:A lactate permease was biochemically identified in Candida albicans RM1000 presenting the following kinetic parameters at pH 5.0: Km 0.33+/-0.09 mM and Vmax 0.85+/-0.06 nmol s(-1) mg dry wt(-1). Lactate uptake was competitively inhibited by pyruvic and propionic acids; acetic acid behaved as a non-competitive substrat...

journal_title：Molecular membrane biology

authors： Soares-Silva I,Paiva S,Kötter P,Entian KD,Casal M

更新日期：2004-11-01 00:00:00

abstract：:Eleven sequenced anion exchanger (AE; band 3) proteins, including five AE1, four AE2 and two AE3 proteins, comprise the anion exchanger family (AEF) of homologous proteins. Eliminating the rat and rabbit proteins that are nearly identical to the corresponding mouse proteins, seven dissimilar members of this family wer...

journal_title：Molecular membrane biology

authors： Espanol MJ,Saier MH Jr

更新日期：1995-04-01 00:00:00

abstract：:Spontaneous membrane adsorption, folding and insertion of the synthetic WALP16 and KALP16 peptides was studied by computer simulations starting from completely extended conformations. The peptides were simulated using an unmodified all-atom force field in combination with an efficient Monte Carlo sampling algorithm. T...

journal_title：Molecular membrane biology

authors： Ulmschneider MB,Ulmschneider JP

更新日期：2008-04-01 00:00:00

abstract：:The assessment of the transverse distribution and mobility of NBD-labelled phospholipid analogues in biological membranes by selective chemical destruction of fluorescent label in the outer monolayer with dithionite has been investigated using resealed erythrocyte ghosts as a model system. The distribution of those an...

journal_title：Molecular membrane biology

authors： Pomorski T,Herrmann A,Zachowski A,Devaux PF,Müller P

更新日期：1994-01-01 00:00:00

abstract：:2B is a 99 amino acid membrane protein encoded by enteroviruses such as polio and coxsackie viruses with two transmembrane domains. The protein is found to make membranes of infected cells permeable. Using a computational approach which positions the models and assesses stability by molecular dynamics (MD) simulations...

journal_title：Molecular membrane biology

authors： Patargias G,Barke T,Watts A,Fischer WB

更新日期：2009-08-01 00:00:00

abstract：:A structural characterization of a synthetic peptide corresponding to the fourth transmembrane domain (M4-TMD) of the gamma-subunit of the nicotinic acetylcholine receptor from Torpedo californica has been undertaken. Solid-state NMR and CD spectroscopy studies indicate that upon reconstitution into lipid vesicles or ...

journal_title：Molecular membrane biology

authors： Williamson PT,Zandomeneghi G,Barrantes FJ,Watts A,Meier BH

更新日期：2005-11-01 00:00:00

abstract：:The Amt/Mep/Rh family of integral membrane proteins comprises ammonium transporters of bacteria, archaea and eukarya, as well as the Rhesus proteins found in animals. They play a central role in the uptake of reduced nitrogen for biosynthetic purposes, in energy metabolism, or in renal excretion. Recent structural inf...

journal_title：Molecular membrane biology

authors： Andrade SL,Einsle O

更新日期：2007-09-01 00:00:00

abstract：:The peptide specificity of both presequence-monolayer interactions and the ability of presequences to induce interbilayer contacts between large unilamellar vesicles was investigated. A range of different synthetic peptides that are documented for their mitochondrial protein import abilities were used for this purpose...

journal_title：Molecular membrane biology

authors： Leenhouts JM,Török Z,Demel RA,de Gier J,de Kruijff B

更新日期：1994-07-01 00:00:00

abstract：:Membrane proteins are intrinsically involved in both human and pathogen physiology, and are the target of 60% of all marketed drugs. During the past decade, advances in the studies of membrane proteins using X-ray crystallography, electron microscopy and NMR-based techniques led to the elucidation of over 250 unique m...

journal_title：Molecular membrane biology

authors： Simmons KJ,Gotfryd K,Billesbølle CB,Loland CJ,Gether U,Fishwick CW,Johnson AP

更新日期：2013-03-01 00:00:00

abstract：:Vascular-targeted drug delivery systems could provide more efficient and effective pharmaceutical interventions for treating a variety of diseases including cardiovascular, pulmonary, inflammatory, and malignant disorders. However, several factors must be taken into account when designing these systems. The diverse bl...

journal_title：Molecular membrane biology

authors： Huang RB,Mocherla S,Heslinga MJ,Charoenphol P,Eniola-Adefeso O

更新日期：2010-08-01 00:00:00

abstract：:The transverse distribution of phosphatidylglycerol (PG) across the inner membrane of Escherichia coli was studied. The oxidation of PG by periodate was used to discriminate between the PG present in the cytoplasmic and in the periplasmic leaflet of the inner membrane. Applied to large unilamellar vesicles derived of ...

journal_title：Molecular membrane biology

authors： Huijbregts RP,de Kroon AI,de Kruijff B

更新日期：1997-01-01 00:00:00

abstract：:Membrane microdomains enriched in cholesterol, sphingolipids (rafts), and specific proteins are involved in important physiological functions. However their structure, size and stability are still controversial. Given that detergent-resistant membranes (DRMs) are in the liquid-ordered state and are rich in raft-like c...

journal_title：Molecular membrane biology

authors： Casadei BR,De Oliveira Carvalho P,Riske KA,Barbosa Rde M,De Paula E,Domingues CC

更新日期：2014-09-01 00:00:00

abstract：:4-Hydroxy-2-nonenal (4-HNE) is a reactive aldehyde and a lipid peroxidation product formed in biological tissues under physiological and pathological conditions. Its concentration increases with oxidative stress and induces deleterious modifications of proteins and membranes. Mitochondrial and cytosolic isoforms of cr...

journal_title：Molecular membrane biology

authors： Maniti O,François-Moutal L,Lecompte MF,Vial C,Lagarde M,Guichardant M,Marcillat O,Granjon T

更新日期：2015-01-01 00:00:00

abstract：:Previous work from our laboratory has established bovine hippocampal membranes as a convenient natural source for studying neuronal receptors such as the G-protein coupled serotonin1A receptor. In this paper, we have explored the organization and dynamics of bovine hippocampal membranes using environment-sensitive and...

journal_title：Molecular membrane biology

authors： Mukherjee S,Kalipatnapu S,Pucadyil TJ,Chattopadhyay A

更新日期：2006-09-01 00:00:00

abstract：:Equilibrative Nucleoside Transporters (SLC29) are a family of proteins that transport nucleosides, nucleobases and nucleoside analogue drugs across cellular membranes. ENT1 is expressed ubiquitously in mammalian tissues and responsible for a significant portion of nucleoside analog drug uptake in humans. Despite the i...

journal_title：Molecular membrane biology

authors： Reyes G,Nivillac NM,Karim MZ,Desouza L,Siu KW,Coe IR

更新日期：2011-09-01 00:00:00

abstract：:The ATP binding cassette (ABC) transporter Atm1p of the mitochondrial inner membrane performs crucial roles in both the biogenesis of cytosolic/nuclear iron-sulfur proteins and cellular iron homeostasis. Since the function of the mitochondrial iron-sulfur cluster (ISC) assembly machinery is also required for these two...

journal_title：Molecular membrane biology

authors： Kuhnke G,Neumann K,Mühlenhoff U,Lill R

更新日期：2006-03-01 00:00:00

abstract：:Significant progress has been made in membrane protein engineering over the last 5 years, based largely on the re-design of existing scaffolds. Engineering techniques that have been employed include direct genetic engineering, both covalent and non-covalent modification, unnatural amino acid mutagenesis and total synt...

journal_title：Molecular membrane biology

authors： Bayley H,Jayasinghe L

更新日期：2004-07-01 00:00:00

abstract：:Treatment of BHK fibroblasts with V. cholerae sialidase for 20 min caused the breakdown of about 70% of total cellular ganglioside GM3 and the production of an approximately equivalent amount of lactosylceramide. On removal of the enzyme, a slow resynthesis of GM3 from lactosylceramide was observed, equivalent to abou...

journal_title：Molecular membrane biology

authors： Sillence DJ,Allan D

更新日期：1998-10-01 00:00:00

abstract：:The transporter SbtA is a high affinity Na+-dependent HCO3- uptake system present in a majority of cyanobacterial clades. It functions in conjunction with CO2 uptake systems and other HCO3- uptake systems to allow cyanobacteria to accumulate high levels of HCO3- used to support efficient photosynthetic CO2 fixation vi...

journal_title：Molecular membrane biology

authors： Price GD,Shelden MC,Howitt SM

更新日期：2011-08-01 00:00:00

abstract：:Membrane proteins are ubiquitous in biology and are key targets for therapeutic development. Despite this, our structural understanding has lagged behind that of their soluble counterparts. This review provides an overview of this important field, focusing in particular on the recent resurgence of electron microscopy ...

journal_title：Molecular membrane biology

authors： Rawson S,Davies S,Lippiat JD,Muench SP

更新日期：2016-03-01 00:00:00

abstract：:Notch receptor signalling plays a central role in development and its misfunction has been linked to a number of diseases. In the cannonical Notch signalling pathway, ligand binding to Notch activates a series of proteolytic cleavages that release the Notch intracellular domain for trafficking to the nucleus, where it...

journal_title：Molecular membrane biology

authors： Wilkin MB,Baron M

更新日期：2005-07-01 00:00:00

abstract：:Glu-269, which is located on the hydrophilic face of putative helix VIII in the lactose permease of Escherichia coli, has been replaced with Asp, Gln or Cys by oligonucleotide-directed, site specific mutagenesis. Cells expressing Asp-269 permease exhibit no lactose accumulation or lactose-induced H+ translocation, but...

journal_title：Molecular membrane biology

authors： Ujwal ML,Sahin-Tóth M,Persson B,Kaback HR

更新日期：1994-01-01 00:00:00