Regulation of the nuclear localization of the human Nedd4-related WWP1 protein by Notch.

abstract：:Time and distance scales in membranes are discussed in relation to the inference of domain structure from spectroscopic measurements. Each type of spectroscopy has a natural time scale set by the magnitude of the interactions that determine the spectral width. For fluid membranes, the lateral diffusion of the lipid mo...

journal_title：Molecular membrane biology

authors： Bloom M,Thewalt JL

更新日期：1995-01-01 00:00:00

abstract：:Small GTPase Rab36 is homologous to Rab34 with 56% amino acid sequence identity. Rab34 was characterized as a Golgi-associated Rab protein and regulates lysosomal positioning through interaction with RILP; however, the properties and functions of Rab36 have not been investigated. To investigate Rab36, we constructed E...

journal_title：Molecular membrane biology

authors： Chen L,Hu J,Yun Y,Wang T

更新日期：2010-01-01 00:00:00

abstract：:Neurotransmitter transporters are regulated through a variety of signal transduction mechanisms which appear to operate in order to maintain appropriate levels of transmitter in the synaptic cleft. One such mechanism is the trafficking of the transporter in association with synaptic vesicle release machinery. This rep...

journal_title：Molecular membrane biology

authors： Horton N,Quick MW

更新日期：2001-01-01 00:00:00

abstract：:The peptide specificity of both presequence-monolayer interactions and the ability of presequences to induce interbilayer contacts between large unilamellar vesicles was investigated. A range of different synthetic peptides that are documented for their mitochondrial protein import abilities were used for this purpose...

journal_title：Molecular membrane biology

authors： Leenhouts JM,Török Z,Demel RA,de Gier J,de Kruijff B

更新日期：1994-07-01 00:00:00

abstract：:At least four different types of interaction between protein transmembrane helices have been described to date. These include the use of charge-pair interactions that can play a positive or negative role in the assembly of multi-subunit complexes such as the T cell receptor, or recruit signal transducing accessory mol...

journal_title：Molecular membrane biology

authors： Harrison PT

更新日期：1996-04-01 00:00:00

abstract：:The eukaryotic endomembrane system (ES) is served by hundreds of dedicated proteins. Experimental characterization of the ES-associated molecular machinery in several model eukaryotes complemented by a recent progress in phylogenomics and comparative genomics have revealed a conserved complex core of the machinery tha...

journal_title：Molecular membrane biology

authors： Elias M

更新日期：2010-11-01 00:00:00

abstract：:Calpain, an intracellular calcium-dependent protease, is activated at cell membranes and cleaves cytoskeletal and submembranous proteins. Calpain is inferred to be a calcium-dependent regulator for cytoskeletal reorganization. Calpastatin, an endogenous calpain inhibitor, inhibits not only the proteolytic activity of ...

journal_title：Molecular membrane biology

authors： Kawasaki H,Kawashima S

更新日期：1996-10-01 00:00:00

abstract：:Notch receptor signalling plays a central role in development and its misfunction has been linked to a number of diseases. In the cannonical Notch signalling pathway, ligand binding to Notch activates a series of proteolytic cleavages that release the Notch intracellular domain for trafficking to the nucleus, where it...

journal_title：Molecular membrane biology

authors： Wilkin MB,Baron M

更新日期：2005-07-01 00:00:00

abstract：:Significant progress has been made in membrane protein engineering over the last 5 years, based largely on the re-design of existing scaffolds. Engineering techniques that have been employed include direct genetic engineering, both covalent and non-covalent modification, unnatural amino acid mutagenesis and total synt...

journal_title：Molecular membrane biology

authors： Bayley H,Jayasinghe L

更新日期：2004-07-01 00:00:00

abstract：:The number of Signal Peptidase I (SPasel) molecules per E. coli cell was determined using western blot techniques. Different strains were found to contain approximately 1000 SPasel molecules per cell during exponential growth. Based on the activity of SPasel in vitro it could be estimated that this amount is sufficien...

journal_title：Molecular membrane biology

authors： van Klompenburg W,Whitley P,Diemel R,von Heijne G,de Kruijff B

更新日期：1995-10-01 00:00:00

abstract：:In Saccharomyces cerevisiae Jen1p is a lactate/proton symporter belonging to the lactate/pyruvate:H(+) symporter subfamily (TC#2.A.1.12.2) of the Major Facilitator Superfamily. We investigated structure-function relationships of Jen1p using a rational mutational analysis based on the identification of conserved amino ...

journal_title：Molecular membrane biology

authors： Soares-Silva I,Paiva S,Diallinas G,Casal M

更新日期：2007-09-01 00:00:00

abstract：:Spontaneous membrane adsorption, folding and insertion of the synthetic WALP16 and KALP16 peptides was studied by computer simulations starting from completely extended conformations. The peptides were simulated using an unmodified all-atom force field in combination with an efficient Monte Carlo sampling algorithm. T...

journal_title：Molecular membrane biology

authors： Ulmschneider MB,Ulmschneider JP

更新日期：2008-04-01 00:00:00

abstract：:The entry of substrates into, and the export of glururonides from, the lumen of hepatic endoplasmic reticulum (ER) in vitro (sealed microsomes) has been measured using radioactivity-labelled materials and a rapid filtration assay. Analysis of liver microsomes from a jaundiced patient showed the accumulation of bilirub...

journal_title：Molecular membrane biology

authors： Waddell ID,Robertson K,Burchell A,Hume R,Burchell B

更新日期：1995-07-01 00:00:00

abstract：:Temperature-composition phase diagrams of binary lipid mixtures under conditions of constant pressure and excess water show that component immiscibility is the rule rather than the exception in these systems. Phase immiscibility is particularly noted in the solid phase but several cases are known where immiscibility i...

journal_title：Molecular membrane biology

authors： Vaz WL

更新日期：1995-01-01 00:00:00

abstract：:Solid-state NMR combined with sample deuteration was used to probe the proximity of the low-affinity substrate D-glucose to its binding site within the Escherichia coli sugar transport protein GalP. Samples of E. coli inner membranes with amplified expression of GalP were incubated in D(2)O with D-[(13)C(6)]glucose an...

journal_title：Molecular membrane biology

authors： Patching SG,Henderson PJ,Sharples DJ,Middleton DA

更新日期：2013-03-01 00:00:00

abstract：:That some membranes restrict certain lipid species to one side of the bilayer and others to the opposite side has been known for two decades. However, how this asymmetric transbilayer distribution is generated and controlled, how many and what type of membranes are so structured, and even the reason for its existence ...

journal_title：Molecular membrane biology

authors： Williamson P,Schlegel RA

更新日期：1994-10-01 00:00:00

abstract：:Hereditary spherocytosis (HS), a common human inherited haemolytic anaemia, is associated with partial deficiency of different erythrocyte membrane proteins. In a subset of dominant HS, a partial membrane expression deficiency of band 3, the erythrocyte anion exchanger (AE1), have previously been characterized, and se...

journal_title：Molecular membrane biology

authors： Dhermy D,Burnier O,Bourgeois M,Grandchamp B

更新日期：1999-10-01 00:00:00

abstract：:Organellar and cytosolic pH homeostasis is central to most cellular processes, including vesicular trafficking, post-translational modification/processing of proteins, and receptor-ligand interactions. SLC9A7 (NHE7) was identified as a unique (Na+, K+)/H+ exchanger that dynamically cycles between the trans-Golgi netwo...

journal_title：Molecular membrane biology

authors： Kagami T,Chen S,Memar P,Choi M,Foster LJ,Numata M

更新日期：2008-08-01 00:00:00

abstract：:The assessment of the transverse distribution and mobility of NBD-labelled phospholipid analogues in biological membranes by selective chemical destruction of fluorescent label in the outer monolayer with dithionite has been investigated using resealed erythrocyte ghosts as a model system. The distribution of those an...

journal_title：Molecular membrane biology

authors： Pomorski T,Herrmann A,Zachowski A,Devaux PF,Müller P

更新日期：1994-01-01 00:00:00

abstract：:Homotypic fusion between early endosomes can be reconstituted in vitro. By using wortmannin and LY294002, inhibitors of phosphatidylinositol (Pl) 3-kinase, a requirement for this activity has been established in order for fusion to proceed efficiently. It has been shown that Pl 3-kinase activity is required downstream...

journal_title：Molecular membrane biology

authors： Mills IG,Jones AT,Clague MJ

更新日期：1999-01-01 00:00:00

abstract：:Palmitoylation is required for the activities of several cancer-associated proteins, making the palmitoyl acyltransferase (PAT) enzymes that catalyze these reactions potential targets for anticancer therapeutics. In this study, we sought to identify and characterize a human PAT with activity toward N-terminally myrist...

journal_title：Molecular membrane biology

authors： Draper JM,Smith CD

更新日期：2010-04-01 00:00:00

abstract：:Cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-regulated C1(-) channel. Malfunction of CFTR causes cystic fibrosis (CF). CFTR belongs to an ATP-binding cassette (ABC) transporter superfamily which includes P-glycoprotein (Pgp), the molecule that is responsible for multidrug resistance in cancer c...

journal_title：Molecular membrane biology

authors： Chen M,Zhang JT

更新日期：1996-01-01 00:00:00

abstract：:Membrane proteins are ubiquitous in biology and are key targets for therapeutic development. Despite this, our structural understanding has lagged behind that of their soluble counterparts. This review provides an overview of this important field, focusing in particular on the recent resurgence of electron microscopy ...

journal_title：Molecular membrane biology

authors： Rawson S,Davies S,Lippiat JD,Muench SP

更新日期：2016-03-01 00:00:00

abstract：:Previous work from our laboratory has established bovine hippocampal membranes as a convenient natural source for studying neuronal receptors such as the G-protein coupled serotonin1A receptor. In this paper, we have explored the organization and dynamics of bovine hippocampal membranes using environment-sensitive and...

journal_title：Molecular membrane biology

authors： Mukherjee S,Kalipatnapu S,Pucadyil TJ,Chattopadhyay A

更新日期：2006-09-01 00:00:00

abstract：:The serotonin1A (5-HT1A) receptors are members of a superfamily of seven transmembrane domain receptors that couple to G-proteins. They appear to be involved in various behavioural and cognitive functions. This paper reports an efficient strategy to solubilize 5-HT1A receptors from bovine hippocampal membranes using t...

journal_title：Molecular membrane biology

authors： Chattopadhyay A,Harikumar KG,Kalipatnapu S

更新日期：2002-07-01 00:00:00

abstract：:Numerous cell surface receptors propagate activation signals to the interior of the cell via tyrosine phosphorylation of transmembrane proteins. This leads to the phosphotyrosine (PiY)-mediated recruitment of cytoplasmic signalling protein complexes which catalyze crucial biochemical signalling reactions. Here we desc...

journal_title：Molecular membrane biology

authors： Sangani D,Venien-Bryan C,Harder T

更新日期：2009-04-01 00:00:00

abstract：:'No cellular organelle has been the subject of as many, as long-lasting or as diverse polemics as the Golgi apparatus'. This statement was made by Whaley almost 30 years ago in the book The Golgi Apparatus and still holds true today, perhaps more then ever. Why is this? How come something as mundane as a series of int...

journal_title：Molecular membrane biology

authors： Elsner M,Hashimoto H,Nilsson T

更新日期：2003-07-01 00:00:00

abstract：:This investigation is aimed at defining the structural requirements for aliphatic polyamines to interact with mitochondrial binding sites, which are relevant for the regulation of the permeability transition and for mitochondrial polyamine uptake. The triamines N-aminoethyl-1,2-diaminoethane (dien) and N-aminopropyl-1...

journal_title：Molecular membrane biology

authors： Dalla Via L,Salvi M,Di Noto V,Stefanelli C,Toninello A

更新日期：2004-03-01 00:00:00

abstract：:Site-directed mutagenesis and site-directed fluorescence spectroscopy demonstrate that Cys148 interacts hydrophobically with the galactosyl moiety of substrates of the lactose permease of Escherichia coli. By taking advantage of the finding that labelling of single-Cys148 permease with the thiol-specific fluorophore 2...

journal_title：Molecular membrane biology

authors： He MM,Kaback HR

更新日期：1998-01-01 00:00:00

abstract：:The cyanelles of the glaucocystophyte alga Cyanophora paradoxa resemble endosymbiotic cyanobacteria in morphology, pigmentation and, especially, in the presence of a peptidoglycan wall situated between the inner and outer envelope membranes. However, it is now clear that cyanelles in fact are primitive plastids. Phylo...

journal_title：Molecular membrane biology

authors： Steiner JM,Löffelhardt W

更新日期：2005-01-01 00:00:00