Abstract:
:The peptide specificity of both presequence-monolayer interactions and the ability of presequences to induce interbilayer contacts between large unilamellar vesicles was investigated. A range of different synthetic peptides that are documented for their mitochondrial protein import abilities were used for this purpose. Both monolayer insertion and vesicle aggregation were found to be strongly dependent on the primary structure of the studied presequence peptides. The combination of monolayer data and results of vesicle aggregation experiments leads to the overall suggestion that monolayer insertion and interbilayer contact formation are mechanistically related. For maximal effects the full length of a presequence peptide is required. The cardiolipin specificity of presequence-induced interbilayer contact formation previously reported was found to be a more general property among presequence peptides. The peptide's ability to induce vesicle-vesicle contacts seems to parallel the efficiency of its import ability into mitochondria. These results lead to an extended hypothesis on the role of presequence-induced contact site formation during the mitochondrial protein import process.
journal_name
Mol Membr Bioljournal_title
Molecular membrane biologyauthors
Leenhouts JM,Török Z,Demel RA,de Gier J,de Kruijff Bdoi
10.3109/09687689409162234subject
Has Abstractpub_date
1994-07-01 00:00:00pages
159-64issue
3eissn
0968-7688issn
1464-5203journal_volume
11pub_type
杂志文章abstract::Eleven sequenced anion exchanger (AE; band 3) proteins, including five AE1, four AE2 and two AE3 proteins, comprise the anion exchanger family (AEF) of homologous proteins. Eliminating the rat and rabbit proteins that are nearly identical to the corresponding mouse proteins, seven dissimilar members of this family wer...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689509027507
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journal_title:Molecular membrane biology
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doi:10.3109/09687689509072429
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pub_type: 杂志文章,评审
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abstract::Neurotransmitter transporters are regulated through a variety of signal transduction mechanisms which appear to operate in order to maintain appropriate levels of transmitter in the synaptic cleft. One such mechanism is the trafficking of the transporter in association with synaptic vesicle release machinery. This rep...
journal_title:Molecular membrane biology
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journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689509072437
更新日期:1995-10-01 00:00:00
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journal_title:Molecular membrane biology
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更新日期:2009-05-01 00:00:00
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journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689609160572
更新日期:1996-01-01 00:00:00
abstract::Site-directed mutagenesis and site-directed fluorescence spectroscopy demonstrate that Cys148 interacts hydrophobically with the galactosyl moiety of substrates of the lactose permease of Escherichia coli. By taking advantage of the finding that labelling of single-Cys148 permease with the thiol-specific fluorophore 2...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689809027513
更新日期:1998-01-01 00:00:00
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journal_title:Molecular membrane biology
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doi:10.3109/09687689409161024
更新日期:1994-01-01 00:00:00
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更新日期:2010-10-01 00:00:00
abstract::Spontaneous membrane adsorption, folding and insertion of the synthetic WALP16 and KALP16 peptides was studied by computer simulations starting from completely extended conformations. The peptides were simulated using an unmodified all-atom force field in combination with an efficient Monte Carlo sampling algorithm. T...
journal_title:Molecular membrane biology
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更新日期:2008-04-01 00:00:00
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journal_title:Molecular membrane biology
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doi:10.3109/09687689709044325
更新日期:1998-10-01 00:00:00
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journal_title:Molecular membrane biology
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doi:10.3109/09687688.2011.593049
更新日期:2011-08-01 00:00:00
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journal_title:Molecular membrane biology
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doi:10.1080/09687860601127770
更新日期:2007-05-01 00:00:00
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journal_title:Molecular membrane biology
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更新日期:2005-07-01 00:00:00
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journal_title:Molecular membrane biology
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journal_title:Molecular membrane biology
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doi:10.3109/09687689509038506
更新日期:1995-01-01 00:00:00
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journal_title:Molecular membrane biology
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doi:10.3109/09687689809074524
更新日期:1998-07-01 00:00:00
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journal_title:Molecular membrane biology
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