Abstract:
:Organellar and cytosolic pH homeostasis is central to most cellular processes, including vesicular trafficking, post-translational modification/processing of proteins, and receptor-ligand interactions. SLC9A7 (NHE7) was identified as a unique (Na+, K+)/H+ exchanger that dynamically cycles between the trans-Golgi network (TGN), endosomes and the plasma membrane. Here we have used mass spectrometry to explore the affinity-captured interactome of NHE7, leading to the identification of cytoskeletal proteins, cell adhesion molecules, membrane transporters, and signaling molecules. Among these binding proteins, calcium-calmodulin, but not apo-calmodulin, binds to NHE7 and regulates the organellar transporter activity. Vimentin was co-immunoprecipitated with endogenous NHE7 protein in human breast cancer MDA-MB-231 cells. A sizable population of NHE7 relocalized to focal complexes in migrating cells and showed colocalization with vimentin and actin in focal complexes. Among the NHE7-binding proteins identified, CD44, a cell surface glycoprotein receptor for hyaluronate and other ligands, showed regulated interaction with NHE7. Pretreatment of the cells with phorbol ester facilitated the NHE7-CD44 interaction and the lipid raft association of CD44. When lipid rafts were chemically disrupted, the NHE7-CD44 interaction was markedly reduced. These results suggest potential dual roles of NHE7 in intracellular compartments and subdomains of cell-surface membranes.
journal_name
Mol Membr Bioljournal_title
Molecular membrane biologyauthors
Kagami T,Chen S,Memar P,Choi M,Foster LJ,Numata Mdoi
10.1080/09687680802263046subject
Has Abstractpub_date
2008-08-01 00:00:00pages
436-47issue
5eissn
0968-7688issn
1464-5203pii
795318444journal_volume
25pub_type
杂志文章abstract::The transporter SbtA is a high affinity Na+-dependent HCO3- uptake system present in a majority of cyanobacterial clades. It functions in conjunction with CO2 uptake systems and other HCO3- uptake systems to allow cyanobacteria to accumulate high levels of HCO3- used to support efficient photosynthetic CO2 fixation vi...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2011.593049
更新日期:2011-08-01 00:00:00
abstract::Evidence was recently reported that the cysteine proteinase inhibitor, cystatin C, is highly expressed by cultured human retinal pigment epithelial (RPE) cells. As a step towards understanding possible functions of this protein associated with the RPE, the localization, targetting and trafficking of cystatin C were in...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680110075101
更新日期:2001-07-01 00:00:00
abstract::Small GTPase Rab36 is homologous to Rab34 with 56% amino acid sequence identity. Rab34 was characterized as a Golgi-associated Rab protein and regulates lysosomal positioning through interaction with RILP; however, the properties and functions of Rab36 have not been investigated. To investigate Rab36, we constructed E...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687680903417470
更新日期:2010-01-01 00:00:00
abstract::Solid-state NMR combined with sample deuteration was used to probe the proximity of the low-affinity substrate D-glucose to its binding site within the Escherichia coli sugar transport protein GalP. Samples of E. coli inner membranes with amplified expression of GalP were incubated in D(2)O with D-[(13)C(6)]glucose an...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2012.743193
更新日期:2013-03-01 00:00:00
abstract::The cyanelles of the glaucocystophyte alga Cyanophora paradoxa resemble endosymbiotic cyanobacteria in morphology, pigmentation and, especially, in the presence of a peptidoglycan wall situated between the inner and outer envelope membranes. However, it is now clear that cyanelles in fact are primitive plastids. Phylo...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/09687860500041411
更新日期:2005-01-01 00:00:00
abstract::Loss of epithelial cell polarity, which can arise following disruption of tight junctions (TJs), is a precursor to the care-fully orchestrated removal of moribund cells from epithelia in apoptosis. Ordinarily, this cycle of events has minimally disruptive effects on the function of the epithelial barrier, but some age...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/0968768021000061150
更新日期:2003-01-01 00:00:00
abstract::In Saccharomyces cerevisiae Jen1p is a lactate/proton symporter belonging to the lactate/pyruvate:H(+) symporter subfamily (TC#2.A.1.12.2) of the Major Facilitator Superfamily. We investigated structure-function relationships of Jen1p using a rational mutational analysis based on the identification of conserved amino ...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680701342669
更新日期:2007-09-01 00:00:00
abstract::Insolubility of membrane components in non-ionic detergents such as Triton X-100 at low temperature is a widely used biochemical criterion to identify, isolate and characterize membrane domains. In this work, we monitored the detergent insolubility of the serotonin(1A) receptor in CHO cell membranes and its modulation...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2012.688147
更新日期:2012-11-01 00:00:00
abstract::In hair cells of the inner ear, phosphatidylserine (PS), detected with fluorescent annexin V labeling, was rapidly exposed on the external leaflet of apical plasma membranes upon dissection of the organ of Corti. PS externalization was unchanged by caspase inhibition, suggesting that externalization did not portend ap...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860600926883
更新日期:2007-01-01 00:00:00
abstract::PagP is a bacterial outer membrane protein consisting of an 8 stranded transmembrane beta-barrel and an N-terminal alpha-helix. It is an enzyme which catalyses transfer of a palmitoyl chain from a phospholipid to lipid A. Molecular dynamics simulations have been used to compare the dynamic behaviour in simulations sta...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680902788967
更新日期:2009-05-01 00:00:00
abstract::The ATP binding cassette (ABC) transporter Atm1p of the mitochondrial inner membrane performs crucial roles in both the biogenesis of cytosolic/nuclear iron-sulfur proteins and cellular iron homeostasis. Since the function of the mitochondrial iron-sulfur cluster (ISC) assembly machinery is also required for these two...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860500473630
更新日期:2006-03-01 00:00:00
abstract::A structural characterization of a synthetic peptide corresponding to the fourth transmembrane domain (M4-TMD) of the gamma-subunit of the nicotinic acetylcholine receptor from Torpedo californica has been undertaken. Solid-state NMR and CD spectroscopy studies indicate that upon reconstitution into lipid vesicles or ...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860500370653
更新日期:2005-11-01 00:00:00
abstract::Trypsin activation of Cry4B, a 130-kDa Bacillus thuringiensis (Bt) protein, produces a 65-kDa toxin active against mosquito larvae. The active toxin is made of two protease resistant-products of ca. 45 kDa and ca. 20 kDa. The cloned 21-kDa fragment consisting of the N-terminal region of the toxin was previously shown ...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680310001625792
更新日期:2004-01-01 00:00:00
abstract::Neurotransmitter transporters are regulated through a variety of signal transduction mechanisms which appear to operate in order to maintain appropriate levels of transmitter in the synaptic cleft. One such mechanism is the trafficking of the transporter in association with synaptic vesicle release machinery. This rep...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::NupG from Escherichia coli is the archetype of a family of nucleoside transporters found in several eubacterial groups and has distant homologues in eukaryotes, including man. To facilitate investigation of its molecular mechanism, we developed methods for expressing an oligohistidine-tagged form of NupG both at high ...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860400003941
更新日期:2004-09-01 00:00:00
abstract::2B is a 99 amino acid membrane protein encoded by enteroviruses such as polio and coxsackie viruses with two transmembrane domains. The protein is found to make membranes of infected cells permeable. Using a computational approach which positions the models and assesses stability by molecular dynamics (MD) simulations...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680903164101
更新日期:2009-08-01 00:00:00
abstract::The eukaryotic endomembrane system (ES) is served by hundreds of dedicated proteins. Experimental characterization of the ES-associated molecular machinery in several model eukaryotes complemented by a recent progress in phylogenomics and comparative genomics have revealed a conserved complex core of the machinery tha...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687688.2010.521201
更新日期:2010-11-01 00:00:00
abstract::Ionotropic glutamate receptors belong to the superfamily of P-loop channels as well as K(+), Na(+), and Ca(2+) channels. However, the structural similarity between ion channels of the glutamate receptors and K(+) channels is a matter of discussion. The aim of this study was to analyze differences between the structure...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860601008806
更新日期:2007-03-01 00:00:00
abstract::Time and distance scales in membranes are discussed in relation to the inference of domain structure from spectroscopic measurements. Each type of spectroscopy has a natural time scale set by the magnitude of the interactions that determine the spectral width. For fluid membranes, the lateral diffusion of the lipid mo...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687689509038489
更新日期:1995-01-01 00:00:00
abstract::Nedd4 family ubiquitin ligases regulate trafficking and degradation of numerous target substrates in different cellular compartments, including at the plasma membrane, in endosomes, in the secretory pathway and in the nucleus. WWP1 is a Nedd4 family protein closely related to mouse Itch and Drosophila Su(dx), both of ...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860600665010
更新日期:2006-05-01 00:00:00
abstract::The number of structures of integral membrane proteins from higher eukaryotes is steadily increasing due to a number of innovative protein engineering and crystallization strategies devised over the last few years. However, it is sobering to reflect that these structures represent only a tiny proportion of the total n...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687688.2012.703703
更新日期:2013-02-01 00:00:00
abstract::This investigation is aimed at defining the structural requirements for aliphatic polyamines to interact with mitochondrial binding sites, which are relevant for the regulation of the permeability transition and for mitochondrial polyamine uptake. The triamines N-aminoethyl-1,2-diaminoethane (dien) and N-aminopropyl-1...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680310001654916
更新日期:2004-03-01 00:00:00
abstract::Recent advances have revealed much about the signaling events and molecular components associated with autophagy. Although it is clear that there are multiple points of intersection and connection between autophagy and known vesicular membrane transport processes between membrane compartments, autophagy is modulated b...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2013.850178
更新日期:2013-12-01 00:00:00
abstract::The assessment of the transverse distribution and mobility of NBD-labelled phospholipid analogues in biological membranes by selective chemical destruction of fluorescent label in the outer monolayer with dithionite has been investigated using resealed erythrocyte ghosts as a model system. The distribution of those an...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689409161028
更新日期:1994-01-01 00:00:00
abstract::Glu-269, which is located on the hydrophilic face of putative helix VIII in the lactose permease of Escherichia coli, has been replaced with Asp, Gln or Cys by oligonucleotide-directed, site specific mutagenesis. Cells expressing Asp-269 permease exhibit no lactose accumulation or lactose-induced H+ translocation, but...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689409161024
更新日期:1994-01-01 00:00:00
abstract::Elucidating the detailed mechanism of activation of membrane protein receptors and their ligand binding is essential for structure-based drug design. Membrane protein crystal structure analysis successfully aids in understanding these fundamental molecular interactions. However, protein crystal structure analysis of t...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2014.923588
更新日期:2014-08-01 00:00:00
abstract::Cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-regulated C1(-) channel. Malfunction of CFTR causes cystic fibrosis (CF). CFTR belongs to an ATP-binding cassette (ABC) transporter superfamily which includes P-glycoprotein (Pgp), the molecule that is responsible for multidrug resistance in cancer c...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689609160572
更新日期:1996-01-01 00:00:00
abstract::Microorganisms that survive in natural environments form organized multicellular communities, biofilms and colonies with specific properties. During stress and nutrient limitation, slow growing and senescent cells in such communities retain vital processes by maintaining plasma membrane integrity and retaining the abi...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680902893130
更新日期:2009-05-01 00:00:00
abstract::A group of prenyltransferases produce linear lipids by catalyzing consecutive condensation reactions of farnesyl diphosphate (FPP) with specific numbers of isopentenyl diphosphate (IPP), a common building block of isoprenoid compounds. Depending on the stereochemistry of the double bonds formed during IPP condensation...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687688.2012.674162
更新日期:2012-11-01 00:00:00
abstract::The present review introduces the concept of 'non-permanent membrane proteins', to encompass the wide variety of proteins that are not found in a stable membrane-bound form under physiological conditions, yet they interact with the membrane at some stage of their specific course of action. Non-permanent membrane prote...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/0968768021000035078
更新日期:2002-10-01 00:00:00