当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Generation of an ABCG2(GFPn-puro) transgenic line--a tool to study ABCG2 expression in mice.

Generation of an ABCG2(GFPn-puro) transgenic line--a tool to study ABCG2 expression in mice.

Abstract：

:The ATP-binding cassette (ABC) transporter 2 (ABCG2) is expressed by stem cells in many organs and in stem cells of solid tumors. These cells are isolated based on the side population (SP) phenotype, a Hoechst 3342 dye efflux property believed to be conferred by ABCG2. Because of the limitations of this approach we generated transgenic mice that express Nuclear GFP (GFPn) coupled to the Puromycin-resistance gene, under the control of ABCG2 promoter/enhancer sequences. We show that ABCG2 is expressed in neural progenitors of the developing forebrain and spinal cord and in embryonic and adult endothelial cells of the brain. Using the neurosphere assay, we isolated tripotent ABCG2-expressing neural stem cells from embryonic mouse brain. This transgenic line is a powerful tool for studying the expression of ABCG2 in many tissues and for performing functional studies in different experimental settings.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Orford M,Mean R,Lapathitis G,Genethliou N,Panayiotou E,Panayi H,Malas S

doi

10.1016/j.bbrc.2009.04.089

subject

Has Abstract

pub_date

2009-06-26 00:00:00

pages

199-203

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(09)00807-9

journal_volume

384

pub_type

杂志文章

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