当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF CARDIOLOGY期刊下所有文献 > Characterisation of isoform-specific tryptic peptides of rat cardiac myosin heavy chains using automated liquid chromatography-matrix assisted laser desorption ionisation (LC-MALDI) mass spectrometry.

Characterisation of isoform-specific tryptic peptides of rat cardiac myosin heavy chains using automated liquid chromatography-matrix assisted laser desorption ionisation (LC-MALDI) mass spectrometry.

Abstract：

:Proteomics investigations using 2-dimensional electrophoresis (2-DE) cannot resolve the entire cardiac proteome because some proteins, including myosin heavy chains (MyHC), are insoluble in the buffers required for isoelectric focusing. Here, we report an automated mass spectrometry (MS) method complementary to 2-DE and capable of yielding important additional information. Rat myocardium was homogenised in standard lysis solution and centrifuged to produce a supernatant fraction, suitable for 2-DE. The pelleted fraction, which is normally discarded, was used for the current analysis. Proteins were digested with trypsin and the peptides fractionated by HPLC. Automated spotting of eluent fractions onto 384-well target plates and matrix-assisted laser desorption tandem time of flight (MALDI-ToF/ToF) MS were directed by dedicated software. Peptide ions were fragmented by collision-induced dissociation and the MS/MS spectra searched against the NCBI database using Mascot. This approach confidently identified 13 tryptic peptides specific to cardiac alpha-MyHC and 4 specific to beta-MyHC, which can be used to differentiate these highly homologous protein isoforms in future quantitative MS analyses.

journal_name

Int J Cardiol

journal_title

International journal of cardiology

authors

Burniston JG,Connolly JB

doi

10.1016/j.ijcard.2008.11.058

subject

Has Abstract

pub_date

2010-04-30 00:00:00

pages

363-6

issue

3

eissn

0167-5273

issn

1874-1754

pii

S0167-5273(08)01243-6

journal_volume

140

pub_type

信件

相关文献

INTERNATIONAL JOURNAL OF CARDIOLOGY文献大全
  • Pulmonary congestion evaluated by lung ultrasound predicts decompensation in heart failure outpatients.

    abstract：BACKGROUND:Pulmonary congestion is the main cause of hospital admission among heart failure (HF) patients. Lung ultrasound (LUS) assessment of B-lines has been recently proposed as a reliable and easy tool for evaluating pulmonary congestion. OBJECTIVE:To determine the prognostic value of LUS in predicting adverse eve...

    journal_title：International journal of cardiology

    pub_type： 杂志文章

    doi：10.1016/j.ijcard.2017.02.150

    authors： Miglioranza MH,Picano E,Badano LP,Sant'Anna R,Rover M,Zaffaroni F,Sicari R,Kalil RK,Leiria TL,Gargani L

    更新日期：2017-08-01 00:00:00

  • Functional mitral regurgitation: from normal to pathological anatomy of mitral valve.

    abstract：:Mitral valve (MV) is composed of several structures working in synchrony to open during diastole and close in systole within the high-pressure systemic environment. Its morphological features ensure a normal leaflet closure that prevents regurgitation of blood back into the left atrium causing loss of ventricular pres...

    journal_title：International journal of cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijcard.2011.11.023

    authors： Di Mauro M,Gallina S,D'Amico MA,Izzicupo P,Lanuti P,Bascelli A,Di Fonso A,Bartoloni G,Calafiore AM,Di Baldassarre A,Italian Group of Study for Heart Valve Disease (Italian Society of Cardiology).

    更新日期：2013-03-10 00:00:00

  • Thrombus crossing through a patent foramen ovale.

    abstract：:Paradoxical embolism across a patent foramen ovale (PFO) is a rare clinical entity and the thrombus is rarely caught passing through a PFO. A 65-year-old woman presented with dyspnea and pleuritic chest pain. Lower extremity venous Doppler ultrasound showed bilaterally extensive thrombosis at deep veins. Transthoracic...

    journal_title：International journal of cardiology

    pub_type： 信件

    doi：10.1016/j.ijcard.2007.11.007

    authors： Ozdogru I,Kaya MG,Dogan A,Inanc T,Yalcin A,Oguzhan A

    更新日期：2009-04-03 00:00:00

  • Association between polymorphisms of the HSPB7 gene and Cheyne-Stokes respiration with central sleep apnea in patients with dilated cardiomyopathy and congestive heart failure.

    abstract：BACKGROUND:CSR-CSA is frequent in patients with CHF. Dilated cardiomyopathy (DCM) is a structural heart disease with strong genetic background, yet one of the leading etiological causes of CHF. Studies have showed that the HSPB7 gene is associated with DCM. OBJECTIVES:We aimed to explore the prevalence of polymorphism...

    journal_title：International journal of cardiology

    pub_type： 杂志文章

    doi：10.1016/j.ijcard.2016.07.107

    authors： Wang M,Ding H,Kang J,Hu K,Lu W,Zhou X,Xu L

    更新日期：2016-10-15 00:00:00

  • 30 days and midterm outcomes of patients undergoing percutaneous replacement of aortic valve according to their renal function: a multicenter study.

    abstract：INTRODUCTION:Chronic kidney disease (CKD) constitutes a predictor of adverse events for surgical aortic valve replacement. In patients undergoing TAVI no study was performed to evaluate outcomes according to renal clearance, which represents the most accurate instrument to assess kidney function. METHODS:From January ...

    journal_title：International journal of cardiology

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.ijcard.2012.04.161

    authors： D'Ascenzo F,Moretti C,Salizzoni S,Bollati M,D'Amico M,Ballocca F,Giordana F,Barbanti M,Ussia GP,Brambilla N,Bedogni F,Biondi Zoccai G,Tamburino C,Gaita F,Sheiban I

    更新日期：2013-08-20 00:00:00

  • Right-to-left shunt, atrial septal aneurysm and thrombophilia in patients with cryptogenic stroke or TIA vs. those with venous thrombo-embolism.

    abstract：:Whether intracardiac right-to-left shunt (RLS) is an independent risk factor for cerebrovascular accidents is disputed. In patients with RLS, venous thrombo-embolism (VTE) may predispose to paradoxical embolic events, among which stroke and transient ischemic attack (TIA). Whether genetic or acquired thrombophilia is ...

    journal_title：International journal of cardiology

    pub_type： 信件

    doi：10.1016/j.ijcard.2007.06.072

    authors： Palmieri V,Tufano A,Carmen Bonito M,Martino S,Sabatella M,Di Minno G,Celentano A

    更新日期：2008-10-30 00:00:00

  • Minimally invasive mitral valve annuloplasty confers a long-term survival benefit compared with state-of-the-art treatment in heart failure with functional mitral regurgitation.

    abstract：BACKGROUND:Clinical impact of the minimally invasive surgical mitral valve annuloplasty (MVA) of functional mitral regurgitation (FMR) in systolic heart failure on top of the state-of-the-art standards of care remains controversial. Therefore, we aimed to compare clinical outcomes of isolated MVA using the mini-invasiv...

    journal_title：International journal of cardiology

    pub_type： 杂志文章

    doi：10.1016/j.ijcard.2017.06.029

    authors： Penicka M,Kotrc M,Ondrus T,Mo Y,Casselman F,Vanderheyden M,Van Camp G,Van Praet F,Bartunek J

    更新日期：2017-10-01 00:00:00

  • Clinical course of Chagas' heart disease: a comparison with dilated cardiomyopathy.

    abstract：:This investigation was carried out to compare the clinical course of patients with chronic Chagas' heart disease with that of patients with dilated cardiomyopathy. A total of 125 patients (75 chagasic and 50 nonchagasic) prospectively followed up at the Cardiomyopathy clinic of Santa Casa Hospital from January 1990 to...

    journal_title：International journal of cardiology

    pub_type： 杂志文章

    doi：10.1016/s0167-5273(97)00083-1

    authors： Bestetti RB,Muccillo G

    更新日期：1997-07-25 00:00:00

  • Can the site of origin of ventricular extrasystoles enhance the localisation of exercise-induced ischaemia?

    abstract：:Previous work from the Departments of Cardiology and Nuclear Medicine, Guy's Hospital, London, has enabled an atlas of the electrocardiographic appearances of ectopics from individual ventricular sites to be compiled. This has been used to investigate the relationship between regions of myocardial ischaemia and the si...

    journal_title：International journal of cardiology

    pub_type： 杂志文章

    doi：10.1016/0167-5273(86)90143-9

    authors： Holt P,Brennand-Roper D,Curry PV,Maisey MN

    更新日期：1986-11-01 00:00:00

  • Anabolic treatment with GH, IGF-I, or anabolic steroids in patients with HIV-associated wasting.

    abstract：:Wasting, and particularly loss of metabolically active lean tissue, contributes to increased mortality, accelerated disease progression, and impairment of strength and functional status in patients with HIV infection. A variety of protein anabolic agents, including growth hormone, insulin-like growth factor-I, testost...

    journal_title：International journal of cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/s0167-5273(02)00247-4

    authors： Mulligan K,Schambelan M

    更新日期：2002-09-01 00:00:00

  • High-density mapping of atrial fibrillation in a chronic substrate: evidence for distinct modes of repetitive wavefront propagation.

    abstract：BACKGROUND:Temporal dynamics of electrical wave propagation during AF is unknown. There are reports of transient linking of atrial activation. We aim to characterize temporal dynamics of wave propagation patterns during AF in an established chronically remodeled substrate. METHODS:Bi-atrial epicardial mapping of AF (m...

    journal_title：International journal of cardiology

    pub_type： 杂志文章

    doi：10.1016/j.ijcard.2015.07.057

    authors： Kuklik P,Lau DH,Ganesan AN,Brooks AG,Sanders P

    更新日期：2015-11-15 00:00:00

  • Distinct vascular and metabolic effects of different classes of anti-hypertensive drugs.

    abstract：BACKGROUND:ASCOT-BPLA study demonstrates that in hypertensive subjects, atenolol+bendroflumethiazide therapy is associated with higher incidence of adverse cardiovascular outcomes and developing diabetes than an amlodipine+perindopril regimen. This is not explained by changes in blood pressure alone. We hypothesized th...

    journal_title：International journal of cardiology

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.ijcard.2008.11.017

    authors： Koh KK,Quon MJ,Han SH,Lee Y,Kim SJ,Koh Y,Shin EK

    更新日期：2010-04-01 00:00:00

  • Cardiorespiratory response to exercise before and after acute beta-adrenoreceptor blockade in nonsmokers and chronic smokers.

    abstract：:To evaluate the effects of chronic smoking on exercise performance we studied 5 smokers and 7 nonsmokers of comparable age and physical characteristics. The resting heart rate in smokers (75 +/- 3 beats/min; mean +/- SD) was significantly (P less than 0.01) higher than in nonsmokers (64 +/- 5). During exercise on a bi...

    journal_title：International journal of cardiology

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/0167-5273(86)90034-3

    authors： Penny WJ,Mir MA

    更新日期：1986-06-01 00:00:00

  • A novel haplotype in ApoAI-CIII-AIV gene region is detrimental to Northwest Indians with coronary heart disease.

    abstract：:The present study investigated the genetic variation of 3' flanking region of ApoA-I (PstI), 3' untranslated region of ApoC-III (SstI) and intron 2 of ApoA-IV (XbaI) in 193 angiographically diagnosed CHD patients and 150 CHD negative controls of Punjab, Northwest India. Haplotype analysis reveals that P2-S2-X1 is a su...

    journal_title：International journal of cardiology

    pub_type： 信件

    doi：10.1016/j.ijcard.2007.07.029

    authors： Singh P,Singh M,Kaur TP,Grewal SS

    更新日期：2008-11-28 00:00:00

  • Vascular aging and hypertension: Implications for the clinical application of central blood pressure.

    abstract：:Vascular aging may be responsible for the high residual lifetime risk for hypertension in the middle-aged and elderly individuals. Increased arterial stiffness and wave reflection has been recognized as the dominant hemodynamic manifestations of vascular aging, and both are major determinants of central blood pressure...

    journal_title：International journal of cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijcard.2016.12.170

    authors： Cheng HM,Park S,Huang Q,Hoshide S,Wang JG,Kario K,Park CG,Chen CH,Characteristics on the Management of Hypertension in Asia - Morning Hypertension Discussion Group (COME Asia MHDG).

    更新日期：2017-03-01 00:00:00

  • Therapeutic options of Angiotensin Receptor Neprilysin inhibitors (ARNis) in chronic heart failure with reduced ejection fraction: Beyond RAAS and sympathetic nervous system inhibition.

    abstract：:In heart failure, in addition to the renin-angiotensin-aldosterone system and sympathetic nervous system, the natriuretic peptide (NP) system plays a fundamental role among compensating mechanisms. The NPs undergo rapid enzymatic degradation that limits their vasorelaxant, natriuretic, and diuretic actions. Degradatio...

    journal_title：International journal of cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijcard.2016.04.180

    authors： Volterrani M,Iellamo F,Senni M,Piepoli MF

    更新日期：2017-01-01 00:00:00

  • Cannabis-induced coronary artery thrombosis and acute anterior myocardial infarction in a young man.

    abstract：:Information concerning acute myocardial infarction after cannabis usage is limited and the actual mechanism of cannabis-induced myocardial infarction is not well known. In the report, we described a young man with an acute myocardial infarction and cannabis-induced coronary thrombosis. ...

    journal_title：International journal of cardiology

    pub_type： 信件

    doi：10.1016/j.ijcard.2006.08.012

    authors： Tatli E,Yilmaztepe M,Altun G,Altun A

    更新日期：2007-09-03 00:00:00

  • Extracranial carotid artery stenosis and outcomes of patients undergoing transcatheter aortic valve replacement.

    abstract：BACKGROUND:Transcatheter aortic valve replacement (TAVR) is an alternative to open cardiac surgery in selected patients with severe aortic stenosis (AS). Carotid artery stenosis (CAS) has been associated with an increased risk of stroke following cardiac surgery, although the association between CAS and outcomes follow...

    journal_title：International journal of cardiology

    pub_type： 杂志文章

    doi：10.1016/j.ijcard.2016.11.107

    authors： Ben-Shoshan J,Zahler D,Steinvil A,Banai S,Keren G,Bornstein NM,Finkelstein A,Halkin A

    更新日期：2017-01-15 00:00:00

  • Acute fluoride poisoning alters myocardial cytoskeletal and AMPK signaling proteins in rats.

    abstract：BACKGROUND:Our previous findings revealed that increased oxidative stress, apoptosis and necrosis were implicated in acute fluoride (F-) induced cardiac dysfunction apart from hypocalcemia and hyperkalemia. Cardiac intermediate filaments (desmin and vimentin) and cytoskeleton linker molecule vinculin plays an imperativ...

    journal_title：International journal of cardiology

    pub_type： 杂志文章

    doi：10.1016/j.ijcard.2016.11.221

    authors： Panneerselvam L,Raghunath A,Perumal E

    更新日期：2017-02-15 00:00:00

  • Influence of amiodarone on QT dispersion in patients with life-threatening ventricular arrhythmias and clinical outcome.

    abstract：UNLABELLED:Increased QT dispersion, defined as the difference between the maximum and minimum QT interval on the standard 12-lead electrocardiogram is assumed to reflect regional inhomogeneity of ventricular repolarization and has been shown to be associated with an increased risk of arrhythmic events. The purpose of t...

    journal_title：International journal of cardiology

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/s0167-5273(97)00073-9

    authors： Meierhenrich R,Helguera ME,Kidwell GA,Tebbe U

    更新日期：1997-08-08 00:00:00

  • Cancer anorexia: a model for the understanding and treatment of secondary anorexia.

    abstract：:Under normal conditions, food intake is controlled in the hypothalamus by: (i) transducing metabolic/sensorial inputs arising from the periphery into neuronal response; (ii) integrating the information originating from different tissues; and (iii) triggering the appropriate feeding responses. Thus, the anorexia associ...

    journal_title：International journal of cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/s0167-5273(02)00234-6

    authors： Rossi Fanelli F,Laviano A

    更新日期：2002-09-01 00:00:00

  • Left ventricular dysfunction in ADPKD and effects of octreotide-LAR: A cross-sectional and longitudinal substudy of the ALADIN trial.

    abstract：BACKGROUND AND AIM:In autosomal dominant polycystic kidney disease (ADPKD) cardiac abnormalities have been observed before the onset of hypertension or renal dysfunction. We sought to characterize, in ADPKD patients, left ventricular (LV) function and its changes after somatostatin-analogue octreotide-LAR treatment. M...

    journal_title：International journal of cardiology

    pub_type： 杂志文章,多中心研究,随机对照试验

    doi：10.1016/j.ijcard.2018.10.063

    authors： Spinelli L,Pisani A,Giugliano G,Trimarco B,Riccio E,Visciano B,Remuzzi G,Ruggenenti P,ALADIN-Cardiovascular Study Group.

    更新日期：2019-01-15 00:00:00

  • Ergonovine provocative test in Caucasian patients with left ventricular apical ballooning syndrome.

    abstract：:Of a series of 346 consecutive patients with chest pain and no significant coronary stenosis studied with ergonovine provocative test (EPT), 18 fulfilled diagnostic criteria for left ventricular apical ballooning syndrome (LVABS). EPT was negative in all these patients. On the other 328 patients, EPT was positive in 6...

    journal_title：International journal of cardiology

    pub_type： 信件

    doi：10.1016/j.ijcard.2009.05.040

    authors： Martínez-Sellés M,Datino T,Pello AM,Fernández-Avilés F

    更新日期：2010-11-05 00:00:00

  • Implanted defibrillators and primary prevention of sudden cardiac death: where are we today?

    abstract：:Implanted defibrillators have become a mainstream therapy for the prevention of sudden cardiac death (SCD) from ventricular tachyarrhythmias in patients with chronic coronary artery disease. A decade of studies has confirmed the superiority of ICDs over antiarrhythmic drug therapy in prolonging the life of patients wi...

    journal_title：International journal of cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijcard.2003.10.032

    authors： Ray IB,Reddy VY

    更新日期：2005-01-01 00:00:00

  • Amlodipine in patients with stable angina pectoris treated with nitrates and beta-blockers. The influence on exercise tolerance, systolic and diastolic functions of the left ventricle.

    abstract：:The effects of 5 and 10 mg of amlodipine and of placebo were compared in 21 patients with stable angina pectoris and multivessel coronary artery disease. The blind comparison was performed by means of bicycle ergometry and stress echocardiography using esophageal stimulation of the left heart atrium. All patients subs...

    journal_title：International journal of cardiology

    pub_type： 临床试验,杂志文章

    doi：10.1016/0167-5273(92)90137-r

    authors： Meluzín J,Stejfa M,Novák M,Zeman K,Spinarová L,Julínek J,Toman J,Simek P

    更新日期：1992-10-01 00:00:00

  • A pilot study to assess the utility of five established variables to standardize exercise treadmill test reporting.

    abstract：BACKGROUND:The prognostic utility of 5 established variables (functional capacity, Duke treadmill score, chronotropic response to exercise, heart rate recovery, and premature ventricular contractions) together after routine exercise treadmill testing (ETT) has not been determined. METHODS:We assessed the combined prog...

    journal_title：International journal of cardiology

    pub_type： 杂志文章

    doi：10.1016/j.ijcard.2016.12.020

    authors： Krishnaswami A,Ho WK,Kwan WP,Tsou C,Rana JS,Solomon MD,Jiang SF,Jang JJ,Alloggiamento T,Praserthdam AW

    更新日期：2017-03-15 00:00:00

  • Impact of point-of-care pre-procedure creatinine and eGFR testing in patients with ST segment elevation myocardial infarction undergoing primary PCI: The pilot STATCREAT study.

    abstract：BACKGROUND:Contrast-induced acute kidney injury (CI-AKI) is a recognised complication during primary PCI that affects short and long term prognosis. The aim of this study was to assess the impact of point-of-care (POC) pre-PPCI creatinine and eGFR testing in STEMI patients. METHODS:160 STEMI patients (STATCREAT group)...

    journal_title：International journal of cardiology

    pub_type： 杂志文章

    doi：10.1016/j.ijcard.2017.03.147

    authors： Karamasis GV,Hampton-Till J,Al-Janabi F,Mohdnazri S,Parker M,Ioannou A,Jagathesan R,Kabir A,Sayer JW,Robinson NM,Aggarwal RK,Clesham GJ,Gamma RA,Kelly PA,Tang KH,Davies JR,Keeble T

    更新日期：2017-08-01 00:00:00

  • An unusual case of myocarditis.

    abstract：:A 25-year-old woman was admitted to our center for left heart failure. The clinical, electrocardiographic and bioptic findings suggested an acute lymphocytic myocarditis. One month later, paroxysmal hypertension and high urinary excretion of noradrenaline revealed the presence of a pheochromocytoma. ...

    journal_title：International journal of cardiology

    pub_type： 杂志文章

    doi：10.1016/s0167-5273(98)00118-1

    authors： Baratella MC,Menti L,Angelini A,Daliento L

    更新日期：1998-08-01 00:00:00

  • A comparison between the Plateletworks-assay and light transmittance aggregometry for monitoring the inhibitory effects of clopidogrel.

    abstract：:A simple and rapid point-of-care platelet function assay that guides antiplatelet responses to aspirin and clopidogrel may be of great clinical value for the individual tailoring of therapy. The Plateletworks assay is based on single platelet counting and has been described as a promising point-of-care assay for the m...

    journal_title：International journal of cardiology

    pub_type： 信件

    doi：10.1016/j.ijcard.2008.10.046

    authors： van Werkum JW,Kleibeuker M,Postma S,Bouman HJ,Elsenberg EH,ten Berg JM,Hackeng CM

    更新日期：2010-04-01 00:00:00

  • Myocardial ischemia/reperfusion upregulates the transcription of the Neuregulin1 receptor ErbB3, but only postconditioning preserves protein translation: Role in oxidative stress.

    abstract：BACKGROUND:Neuregulin1 (Nrg1) and its receptors ErbB are crucial for heart development and for adult heart structural maintenance and function and Nrg1 has been proposed for heart failure treatment. Infarct size is the major determinant of heart failure and the mechanism of action and the role of each ErbB receptor rem...

    journal_title：International journal of cardiology

    pub_type： 杂志文章

    doi：10.1016/j.ijcard.2017.01.122

    authors： Morano M,Angotti C,Tullio F,Gambarotta G,Penna C,Pagliaro P,Geuna S

    更新日期：2017-04-15 00:00:00