Abstract:
:Crustacean hyperglycemic hormone (CHH) not only plays an important role in the modulation of hemolymph glucose level but also functions in other biological events including molting, reproduction and stress response. Of the six CHHs characterized in Marsupenaeus japonicus, an expression system for recombinant Pej-SGP-VII (rPej-SGP-VII-amide) has not yet been established. Here, we established a procedure using a Nus-tag for solubilization, thereby soluble and biologically active rPej-SGP-VII-amide could successfully be obtained by a simpler procedure than previous ones used for producing other recombinant Pej-SGPs (Pej-SGP-I, III and IV). It was found that rPej-SGP-VII-amide thus obtained had the correct arrangement of intramolecular disulfide bonds and helix-rich secondary structure. The established expression system for rPej-SGP-VII-amide may be applicable for the preparation of other recombinant CHHs.
journal_name
Peptidesjournal_title
Peptidesauthors
Nagai C,Asazuma H,Nagata S,Ohira T,Nagasawa Hdoi
10.1016/j.peptides.2008.07.017subject
Has Abstractpub_date
2009-03-01 00:00:00pages
507-17issue
3eissn
0196-9781issn
1873-5169pii
S0196-9781(08)00320-3journal_volume
30pub_type
杂志文章相关文献
PEPTIDES文献大全abstract::The antinociceptive effect of i.t.-administered Tyr-d-Arg-Phe-β-Ala (TAPA), an N-terminal tetrapeptide analog of dermorphin, was characterized in ddY mice. In the mouse tail-flick test, TAPA administered i.t. produced a potent antinociception. The antinociception induced by TAPA was significantly attenuated by i.t. pr...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2013.10.003
更新日期:2013-12-01 00:00:00
abstract::Effects of Met-enkephalin (Met-ENK) and opioid antagonists on H2O2 release by peritoneal macrophages from DA and AO rats were investigated. Met-ENK increased and decreased H2O2 production by macrophages of DA and AO rats, respectively. These effects were antagonized by low, but not high, concentrations of naloxone and...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(95)02003-f
更新日期:1995-01-01 00:00:00
abstract::Increased plasma levels of neuropeptide Y (NPY)-like immunoreactivity (p-NPY-LI) have been described in hemodialysis (HD) patients. In this investigation the effect of a standard HD on p-NPY-LI, analyzed by radioimmunoassay and reverse-phase high performance liquid chromatography (HPLC), was studied. During dialysis p...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(94)00206-l
更新日期:1995-01-01 00:00:00
abstract::We examined the mechanism through which leptin increases Na(+), K(+)-ATPase activity in the rat kidney. Leptin was infused under anaesthesia into the abdominal aorta proximally to the renal arteries and then Na(+), K(+)-ATPase activity was measured in the renal cortex and medulla. Leptin (1mug/kgmin) increased Na(+), ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2006.08.010
更新日期:2006-12-01 00:00:00
abstract::Although physiological processes related to vascular function differ greatly between resistance arteries and conduit arteries, it is not known whether the effects of calcitonin gene-related peptide (CGRP), a vasodilator neuropeptide, on these arteries differ in humans. In the present study, the conduit portion and the...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(96)00076-9
更新日期:1996-01-01 00:00:00
abstract::Parathyroid hormone (PTH) has been shown to have actions within the brain, suggesting the presence of central PTH receptors. This possibility was examined by determining the binding of 125I-labeled [Nle8,18,Tyr34]bovine PTH to the plasma membranes of rat and rabbit brains. Specific binding of the tracer to membranes o...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(93)90174-f
更新日期:1993-11-01 00:00:00
abstract::Intracerebroventricular injections of rat hypothalamic growth hormone-releasing factor (rhGRF) were examined for effects on food intake in free-feeding rats. It was found that, in picomole doses, rhGRF had a stimulatory effect on food intake. Examination of higher doses of rhGRF revealed a decrease in the facilitatory...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(88)90220-3
更新日期:1988-01-01 00:00:00
abstract::The gut tissues and associated nervous system of the African migratory locust, Locusta migratoria, were found to contain FMRFamide-like immunoreactive (FLI) material throughout the five larval instars and 2 weeks into the adult stage in both males and females. FMRFamide-like immunoreactivity associated with the locust...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2003.08.013
更新日期:2003-10-01 00:00:00
abstract::Motilin-immunopositive cells (Mo cells) are known to be present in the upper small intestine of various species, including man. However, whether Mo cells are present in the rabbit gastrointestinal tract remained to be elucidated. Therefore, this study was designed to investigate the distribution of Mo cells in the rab...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(95)00046-m
更新日期:1995-01-01 00:00:00
abstract::Angiotensin II (Ang II) uptake and transport across monolayers of bovine brain microvessel endothelial cells (BMECs) was demonstrated. Ang II transport was linear up to 2 h, saturable with a K(m) of 1.7 nM, and tended to be polarized with the apical-to-basolateral transport being greater. [3H]Ang II transport was foun...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(98)00054-0
更新日期:1998-01-01 00:00:00
abstract::We studied the ability of NPFF, a FMRFamide-like peptide with certain antiopiate properties, to affect the inhibitory effect of morphine on the electrically induced contraction of guinea pig ileum. Neuropeptide FF had no effect when administered alone but reduced morphine inhibition in a noncompetitive manner. Neurope...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(93)90078-u
更新日期:1993-09-01 00:00:00
abstract::To examine the relationship between glutamate receptors and the action of NC-1900 on a step-through passive avoidance (PA) task in mice, MK-801, an NMDA receptor blocker, and (S)-4-carboxyphenylglycine (4CPG), a group I metabotropic receptor antagonist, were administered intraventricularly (i.c.v.) singly or as co-inj...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2004.12.014
更新日期:2005-05-01 00:00:00
abstract::Glucagon-like peptide-1 (GLP-1) is a proglucagon-derived hormone with cellular protective actions. We hypothesized that GLP-1 would protect the endothelium from injury during inflammation. Our aims were to determine the: (1) effect of GLP-1 on basal microvascular permeability, (2) effect of GLP-1 on increased microvas...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2009.06.019
更新日期:2009-09-01 00:00:00
abstract::Female Zucker lean and obese rats were treated for 14 days with 3.5 micromol/kg oleoyl-estrone (OE) in liposomes (Merlin-2). After 0, 3, 6, 10, and 14 days of treatment, the rats were killed and hypothalamic nuclei (lateral preoptic, median preoptic, paraventricular, ventromedial and arcuate) were used for neuropeptid...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(98)00104-1
更新日期:1998-01-01 00:00:00
abstract::In sheep, arginine vasopressin (AVP) and corticotropin-releasing factor (CRF) stimulate adrenocorticotropic hormone (ACTH) secretion. Increased hypothalamic secretion of these peptides at the end of gestation initiates parturition in this species. We hypothesized that CRF and/or AVP content in the fetal hypothalamus i...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(95)02060-8
更新日期:1996-01-01 00:00:00
abstract::We investigated the effect of surgical castration of male rats on the binding of [Tyr(125I)10]VIP to receptors on the anterior pituitary gland, superior mesenteric artery, brain, liver, and prostate gland. In anterior pituitary membranes the maximum number of VIP binding sites was increased whereas binding affinity wa...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(90)90146-v
更新日期:1990-11-01 00:00:00
abstract::The gut-derived hormone peptide YY (PYY) is most commonly known for its effect on satiety, decreasing food intake and body weight in animals and humans. However, PYY is also involved in a wide range of digestive functions including regulating insulin secretion and glucose homeostasis. Over the last few years, there ha...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2006.07.031
更新日期:2007-02-01 00:00:00
abstract::The major amyloid-beta (Abeta) peptides found in the brain of familial and late onset Alzheimer's disease include the full-length Abeta1-42 and N-terminally truncated, pyroglutamylated peptides Abetap3-42 and Abetap11-42. The biophysical properties of Abeta1-42 have been extensively studied, yet little is known about ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2009.01.027
更新日期:2009-05-01 00:00:00
abstract::The in vitro interaction between five polycationic peptides, buforin II, cecropin P1, indolicidin, magainin II, and ranalexin, and several clinically used antimicrobial agents was evaluated against several clinical isolates of Gram-positive and Gram-negative aerobic bacteria, using the microbroth dilution method. The ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(00)00254-0
更新日期:2000-08-01 00:00:00
abstract::To determine if different subtypes of mu-opioid receptors were involved in antinociception induced by endomorphin-1 and endomorphin-2, the effect of pretreatment with various mu-opioid receptor antagonists beta-funaltrexamine, naloxonazine and 3-methylnaltrexone on the inhibition of the paw-withdrawal induced by endom...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(02)00016-5
更新日期:2002-05-01 00:00:00
abstract::Tachykinins are multifunctional neuropeptides that have been identified in vertebrates as well as invertebrates. The C-terminal FXGXRa-motif constitutes the consensus active core region of invertebrate tachykinins. In Drosophila, two putative G protein-coupled tachykinin receptors have been cloned: DTKR and NKD. This ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2006.06.014
更新日期:2007-01-01 00:00:00
abstract::Echinometra lucunter is an abundant sea urchin found in Brazilian waters. Accidents caused by this animal are common and are characterized by the penetration of the spines in the skin, which raises an inflammatory reaction through mechanical trauma as well as by the presumable action of toxins. Additionally, there hav...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2013.07.031
更新日期:2014-03-01 00:00:00
abstract::The thyroid gland is known to harbor cholinergic and VIPergic nerves. In the present study, the influences of cholinergic stimulation by carbachol, cholinergic blockade by methylatropine and stimulation with various VIP sequences on basal, TSH-induced and VIP-induced thyroid hormone section were investigated in vivo i...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(85)90157-3
更新日期:1985-07-01 00:00:00
abstract::The involvement of the hypothalamic beta-endorphinergic system in behavioral processes has previously been studied in adult rats. In the present report, we studied the effects of undernutrition and of inhibitory avoidance training on the hypothalamic beta-endorphin-like immunoreactivity of 21-day-old and adult rats. R...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(88)90117-9
更新日期:1988-07-01 00:00:00
abstract::The aim of the present study was to correlate the development of the renin angiotensin system (RAS) in the kidney of the rat with the development of genetic hypertension. Immature (1-week-old) and adult (12-week-old) normotensive Wistar-Kyoto (WKY) and spontaneously hypertensive kidney rats (SHR) were used for quantif...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(94)00150-5
更新日期:1995-01-01 00:00:00
abstract::The antibacterial activity of peptides without disulfide bridges, spanning the carboxy-terminal segment of arthropod defensins, has been investigated. Although all the peptides have net positive charges, they exhibited varying antibacterial potencies and spectra. Atomic force and fluorescence microscopic analyses indi...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2006.06.010
更新日期:2006-11-01 00:00:00
abstract::Spasmolytic polypeptide (SP) and intestinal trefoil factor (ITF) are trefoil peptides expressed by gut mucus cells. Using specific antisera we have quantified and characterized the molecular forms and distribution of these peptides in the rat gut. SP predominates in the gastric antrum as a 12 kDa form. ITF (7 kDa) is ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(95)00070-z
更新日期:1995-01-01 00:00:00
abstract::A simple and computationally nonintensive technique based on principal component analysis of 3-dimensional fields to derive theoretical descriptors is presented. The descriptors are then applied to a quantitative structure-activity relationship study on bradykinin potentiating pentapeptides. ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(91)90198-x
更新日期:1991-11-01 00:00:00
abstract::Glucagon-like-peptide-1 (GLP-1) is a proglucagon-derived peptide expressed in the intestinal enteroendocrine-L cells and released after meal ingestion. GLP-1 reduces postprandial glycemia not only by its hormonal effects, but also by its inhibitory effects on gastrointestinal motility. Recently, we showed that GLP-1 a...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2010.09.028
更新日期:2011-01-01 00:00:00
abstract::In order to study a possible direct action of LH-RH analogs on the pituitary lactotrophs, we investigated the effect of long-term in vivo pretreatment with D-Trp-6-LH-RH on in vitro secretion of PRL and luteinizing hormone (LH) by the pituitary glands from male and female rats. In vivo pretreatment with D-Trp-6-LH-RH ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(85)90333-x
更新日期:1985-09-01 00:00:00