Regulation of uncoupling protein 2 expression by long-chain fatty acids and hormones in bovine mammary epithelial cells.

abstract：:Ubiquitylation is an essential posttranslational protein modification that is implicated in a diverse array of cellular functions. Although cells contain eight structurally distinct types of polyubiquitin chains, detailed function of several chain types including K29-linked chains has remained largely unclear. Current...

journal_title：Biochemical and biophysical research communications

authors： Tsuchiya H,Tanaka K,Saeki Y

更新日期：2013-06-28 00:00:00

abstract：:[3H]cyclo(His-Pro) bound with high affinity (59 nM) to a single class of sites in rat liver plasma membranes, without significant tracer degradation during equilibration for 60 min at 0 degrees C. Binding was specific and saturable (3.9 pmol/mg protein), and were increased by the addition of K+, Mg++ and Na+ at optima...

journal_title：Biochemical and biophysical research communications

authors： Mori M,Yamada M,Yamaguchi M,Suzuki M,Ohshima K,Kobayashi I,Kobayashi S

更新日期：1986-01-14 00:00:00

abstract：:Antibody-based targeted therapy of cancers requires the antibody targeting of specific molecules inducing tumor cells apoptosis or death. Angiopoietin-2 (Agn-2) and translocator protein (TSPO) are identified as potential target molecules for glioblastoma therapy. The single chain anti-Agn-2 antibody (Anag-2) and anti-...

journal_title：Biochemical and biophysical research communications

authors： Li J,Zhang Z,Lv L,Qiao H,Chen X,Zou C

更新日期：2016-04-01 00:00:00

abstract：:The application of Fast Atom Bombardment Mass Spectrometry to the measurement of small differences in molecular weight is discussed in relation to the spectra of bovine proinsulin and a genetic variant. ...

journal_title：Biochemical and biophysical research communications

authors： Cottrell JS,Frank BH

更新日期：1985-03-29 00:00:00

abstract：:Complementation studies, using fused cell lines from patients with peroxisomal disorders, have shown correction of defective plasmalogen synthesis and phytanic acid oxidation as well as an increase in the number of peroxisomes. At least six complementation groups have been reported. We demonstrate here that complement...

journal_title：Biochemical and biophysical research communications

authors： McGuinness MC,Moser AB,Moser HW,Watkins PA

更新日期：1990-10-15 00:00:00

abstract：:The ubiquitous eukaryotic protein kinase CKII (casein kinase II) has been found to interact with a number of cellular proteins, either through the catalytic subunit or the regulatory subunit. Using the yeast two-hybrid screening method, we found that the catalytic subunit of Drosophila melanogaster CKII (DmCKII) inter...

journal_title：Biochemical and biophysical research communications

authors： Zhao W,Bidwai AP,Glover CV

更新日期：2002-10-18 00:00:00

abstract：:Endostatin, a natural angiogenesis inhibitor, had been identified for years. It opened a new approach for cancer therapy. Sequence analysis revealed that endostatin is the NC1 domain (non-triple-helical domain) of collagen XVIII. In this report, the cDNA of NC1 domain of type VIII collagen (alpha 1) was cloned and exp...

journal_title：Biochemical and biophysical research communications

authors： Xu R,Yao ZY,Xin L,Zhang Q,Li TP,Gan RB

更新日期：2001-11-23 00:00:00

abstract：:A novel method using an amplifier with a cantilever and gold nanoparticles successfully to extend the length of the target for the specific and high sensitive detection of DNA was reported. When the size of gold nanoparticle is 50 nm, a sensitivity of 10(-15)M for the single base mutation detection has been achieved. ...

journal_title：Biochemical and biophysical research communications

authors： Liu T,Tang J,Han M,Jiang L

更新日期：2003-04-25 00:00:00

abstract：:The interaction of transketolase with its acceptor substrate, ribose 5-phosphate, has been studied. The active centers of the enzyme were shown to be functionally nonequivalent with respect to ribose 5-phosphate binding. Under the conditions where only one out of the two active centers of transketolase is functional, ...

journal_title：Biochemical and biophysical research communications

authors： Yurshev VA,Sevostyanova IA,Solovjeva ON,Zabrodskaya SV,Kochetov GA

更新日期：2007-10-05 00:00:00

abstract：:A folded synthetic peptide analog of NDF (NDF5), which has the 52 amino acid EGF-like domain of NDF alpha 2, has been characterized. The folded peptide stimulates tyrosine phosphorylation of Her2, Her3 and Her4 in breast cancer cells and competes with low affinity with full-length NDF alpha 2 for binding to the cells,...

journal_title：Biochemical and biophysical research communications

authors： Kita Y,Mayer J,Zamborelli T,Hara S,Rohde M,Watson E,Koski R,Ratzkin B,Nicolson M

更新日期：1995-05-16 00:00:00

abstract：:Gene delivery has become an important tool for biological research and gene therapy trials. Lentiviral vector (LV) mediated gene transfer is a preferred approach for stable, sustained transgenic expression. We report here step-wise development of an Indian human immunodeficiency virus type 2 (HIV-2) isolate derived th...

journal_title：Biochemical and biophysical research communications

authors： Santhosh CV,Tamhane MC,Kamat RH,Patel VV,Mukhopadhyaya R

更新日期：2008-07-04 00:00:00

abstract：BACKGROUND:Adiponectin (APN) is an adipocyte-derived bioactive molecule with anti-diabetic and anti-atherogenic properties. Although anti-diabetic effects are mostly mediated by the adiponectin receptors AdipoR1 and AdipoR2, the anti-atherogenic mechanisms have not been fully elucidated. METHODS AND RESULTS:In this st...

journal_title：Biochemical and biophysical research communications

authors： Yamamoto H,Kuroda N,Uekita H,Kochi I,Matsumoto A,Niinaga R,Funahashi T,Shimomura I,Kihara S

更新日期：2016-02-05 00:00:00

abstract：:The effects of heparin (180 micrograms/ml) on steady state mRNA levels for fibronectin, thrombospondin, actin and collagen types I and III were investigated in human umbilical artery smooth muscle cells. Heparin caused a 120% increase in thrombospondin mRNA levels and a 60% and 180% increase in the mRNA levels of proc...

journal_title：Biochemical and biophysical research communications

authors： Lyons-Giordano B,Brinker JM,Kefalides NA

更新日期：1989-08-15 00:00:00

abstract：:Chromatin spatial organization is essential for transcriptional modulation and stabilization. The pattern of DNA distal interplay form the multiple topological associating domains (TADs), and further assemble the functional compartmentalization with open and expression-active chromatin ("A" compartments) or closed and...

journal_title：Biochemical and biophysical research communications

authors： Gong W,Liu Y,Qu H,Liu A,Sun P,Wang X

更新日期：2019-03-19 00:00:00

abstract：:The Rep proteins of some plasmid replicons have two functions. Dimers bind to the operator sequences acting as auto-repressors, whereas monomers bind to the iterons to initiate replication of DNA. The ColE2 Rep proteins are present mostly in a dimeric form with some multimers larger than dimers in solution, while the ...

journal_title：Biochemical and biophysical research communications

authors： Han M,Aoki K,Yagura M,Itoh T

更新日期：2007-02-09 00:00:00

abstract：:Reversion of adenine auxotrophic strains of D. radiodurans by cross-links in DNA was studied. Mutation was induced in a wild-type but not in a mitomycin C sensitive mutant after irradiation with near UV (approximately 365nm) in the presence of 4,5',8-trimethylpsoralen (TMP). To get information on implication of cross-...

journal_title：Biochemical and biophysical research communications

authors： Yatagai F,Kitayama S

更新日期：1983-04-29 00:00:00

abstract：:The short-term incubation of HeLa cells in the presence of diferric transferrin or ferricyanide, which are reduced externally by the transplasma membrane reductase, produces a stoichiometric decrease in NADH and increase in NAD+, which is stimulated by insulin. The NADP/NADPH ratio does not change during 15 min incuba...

journal_title：Biochemical and biophysical research communications

authors： Navas P,Sun IL,Morré DJ,Crane FL

更新日期：1986-02-26 00:00:00

abstract：:Skeletal tissue contains a network of nerve fibers expressing several neuropeptides, including vasoactive intestinal peptide (VIP) and the related peptide pituitary adenylate cyclase activating peptide (PACAP). These peptides have been demonstrated to regulate osteoclast formation and osteoclast activity. Using atomic...

journal_title：Biochemical and biophysical research communications

authors： Ransjö M,Lie A,Mukohyama H,Lundberg P,Lerner UH

更新日期：2000-08-02 00:00:00

abstract：:This study reports the X-ray crystallographic structure of the glycyl-tRNA synthetase (GlyRS) of Nanoarchaeum equitans - a hyperthermophilic archaeal species. This is the first archaeal GlyRS crystal structure elucidated. The GlyRS comprises an N-terminal catalytic domain and a C-terminal anticodon-binding domain with...

journal_title：Biochemical and biophysical research communications

authors： Fujisawa A,Toki R,Miyake H,Shoji T,Doi H,Hayashi H,Hanabusa R,Mutsuro-Aoki H,Umehara T,Ando T,Noguchi H,Voet A,Park SY,Tamura K

更新日期：2019-04-02 00:00:00

abstract：:The smallest protein of hepatitis B virus, HBX, has been implicated in the development of liver diseases by interfering with normal cellular processes. Its role in cell proliferation has been unclear as both pro-apoptotic and anti-apoptotic activities have been reported. We showed molecular evidence that HBX induced a...

journal_title：Biochemical and biophysical research communications

authors： Lu YW,Chen WN

更新日期：2005-12-23 00:00:00

abstract：:The role of small ras homologous GTP-binding proteins in the regulation of smooth muscle contractility has become increasingly apparent but there is still little information about the presence of these proteins in human uterine smooth muscle. Messenger RNAs for p21-activated protein kinase isoforms (PAK1, PAK2, and PA...

journal_title：Biochemical and biophysical research communications

authors： Moore F,Da Silva C,Wilde JI,Smarason A,Watson SP,López Bernal A

更新日期：2000-03-16 00:00:00

abstract：:Isolated rat hepatocytes exposed to CCl4 showed a dramatic decrease in [32P] incorporation into proteins which was evident as early as 5 min after the haloalkane addition. DEAE cellulose separation of protein kinases present in both particulated and cytosolic fractions of hepatocytes revealed that only the calcium and...

journal_title：Biochemical and biophysical research communications

authors： Poli G,Albano E,Dianzani MU,Melloni E,Pontremoli S,Marinari UM,Pronzato MA,Cottalasso D

更新日期：1988-06-16 00:00:00

abstract：:From the inner shoots of the chive Allium tuberosum, a single-chained protein with a molecular weight of 36 kDa and an N-terminal sequence manifesting resemblance to chitinases but lacking in cysteine residues characteristic of a cysteine-rich domain present in chitinases of other Allium species, was purified. The iso...

journal_title：Biochemical and biophysical research communications

authors： Lam YW,Wang HX,Ng TB

更新日期：2000-12-09 00:00:00

abstract：:We have investigated the effects of three unrelated topoisomerase 2 inhibitors, genistein, adriamycin, and etoposide, on phosphorylation/activation of the checkpoint kinase Chk2 in normal or ATM-deficient (ATM-) human fibroblasts and in cells overexpressing a catalytically inactive ATR kinase. We demonstrate that geni...

journal_title：Biochemical and biophysical research communications

authors： Théard D,Coisy M,Ducommun B,Concannon P,Darbon JM

更新日期：2001-12-21 00:00:00

abstract：:Kinetics of pinocytosis in Dictyostelium discoideum were investigated over an extended period of time (up to 6 hours) using fluorescein isothiocyanate (FITC)-dextran as a fluid-phase marker. FITC-dextran added to the medium accumulated rapidly inside the cells with a rate of influx equivalent to 9 microns3 of fluid/ce...

journal_title：Biochemical and biophysical research communications

authors： Klein G,Satre M

更新日期：1986-08-14 00:00:00

abstract：:It has been reported that mammalian cells incubated with excess nitric oxide (NO) accumulate p53 protein but concomitantly this p53 loses its capacity for binding to its DNA consensus sequence. As nitration of tyrosine residues in various proteins has been shown to inhibit their functions, we examined whether NO nitra...

journal_title：Biochemical and biophysical research communications

authors： Chazotte-Aubert L,Hainaut P,Ohshima H

更新日期：2000-01-19 00:00:00

abstract：:Dynamic properties of spin-labelled heterosaccharides in the Fc-region of murine monoclonal antihapten immunoglobulin G were studied in model immune complexes (IC) as a function of the IC size. Model IC dimers, trimers and oligomers were formed using bivalent photoaffinity antigens. The ESR spectrum exhibits two compo...

journal_title：Biochemical and biophysical research communications

authors： Kusumi A,Winkelhake JL

更新日期：1986-05-29 00:00:00

abstract：:Lipopolysaccharides (LPS) induces intrahepatic cholestasis and canalicular multispecific organic anion transporter (CMOAT/MRP2) plays a central role in hepatic bilirubin transport. This study examined the role of Kupffer cell in LPS-induced cholestasis. Rats were injected intravenously with LPS. Kupffer cells were ina...

journal_title：Biochemical and biophysical research communications

authors： Nakamura J,Nishida T,Hayashi K,Kawada N,Ueshima S,Sugiyama Y,Ito T,Sobue K,Matsuda H

更新日期：1999-02-05 00:00:00

abstract：:Rat type 1 angiotensin II receptor has two subtypes, namely type 1a and type 1b. The regulation of the expressions of these two subtype receptor mRNAs was studied by using a competitive polymerase chain reaction method. The expression of the type 1a mRNA in the liver was negatively and that of the type 1b mRNA in the ...

journal_title：Biochemical and biophysical research communications

authors： Iwai N,Inagami T,Ohmichi N,Nakamura Y,Saeki Y,Kinoshita M

更新日期：1992-10-15 00:00:00

abstract：:A guinea pig adrenal hydroxysteroid sulfotransferase (gpHST2) has been cloned that is distinct from guinea pig hydroxysteroid sulfotransferase that stereoselectively acts on 3 alpha-hydroxylated neutral steroids (gp3 alpha HST, redesignated gpHST1). The deduced amino acid sequences for gpHST1 and gpHST2 are 86% identi...

journal_title：Biochemical and biophysical research communications

authors： Luu NX,Driscoll WJ,Martin BM,Strott CA

更新日期：1995-12-26 00:00:00