Basic fibroblast growth factor and somatomedin C in human medulloepithelioma.

Abstract:

:Two published cases of medulloepithelioma, a rare malignant pediatric brain tumor composed of a mixture of primitive neuroepithelium and its differentiated neuronal and glial descendants, were examined by immunohistochemical staining for the presence of growth factors. From a panel of antibodies, those identifying basic fibroblast growth factor and insulin-like growth factor I, formerly known as somatomedin C, were strongly immunoreactive within the neuroepithelial cell population of the tumors. Immunoblots of purified recombinant basic fibroblast growth factor and insulin-like growth factor I showed antibody specificity without cross-reactivity. In controls, immunostaining of tissue sections was abolished by preabsorption of primary antibodies with the appropriate growth factor polypeptide antigen. Preabsorption with inappropriate growth factor did not reduce the intensity or alter the distribution of staining. The congruent histologic patterns of immunoreactivities suggest that more than one type of growth factor may be produced by the neuroepithelial component of medulloepithelioma. These growth factors may stimulate proliferation and differentiation of tumor cells by autocrine molecular mechanisms.

journal_name

Cancer

journal_title

Cancer

authors

Shiurba RA,Buffinger NS,Spencer EM,Urich H

doi

10.1002/1097-0142(19910815)68:4<798::aid-cncr28206

subject

Has Abstract

pub_date

1991-08-15 00:00:00

pages

798-808

issue

4

eissn

0008-543X

issn

1097-0142

journal_volume

68

pub_type

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