Abstract：
BACKGROUND AND PURPOSE:Since the Fas/Fas Ligand (FasL) interaction has been recognized as an apoptotic pathway, it eliminates the activated T cells and promotes the survival of grafts. In this study, the effect of FasL transfection of pig chondrocytes on allogeneic transplantation was examined in vitro and in vivo. METHODS:Chondrocytes were isolated from articular and aural cartilages of anesthetized Guizhou Xiang (Gz) pig. The cells were transfected with G418 selected virus, packed from PA317 cells with a constructed plasmid using pig FasL (pGCEN-FasL). The apoptotic effect of FasL transfection was examined on Jurkat cells and activated recipient Gz T cells. The FasL expression was assessed by Western blot and flow cytometry. FasL+chondrocytes-Pluronic F-127 complex was injected into the right abdomen of recipient Gz pig. Histology and morphology of the engineered tissue were examined after 2 and 5 weeks of transplantation. RESULTS:The FasL expression was confirmed in pGCEN-FasL transfected chondrocytes. The expression of FasL of chondrocytes from Gz pig was analyzed by FACS. The apoptosis of Jurkat cells and activated recipient Gz T cells was increased by co-culture with FasL(+) chondrocytes (53.41% and 30.38% (E/T=10:1), in contrast of 32.27% and 13.16% with the control chondrocytes, respectively, P<0.01). FasL(+) chondrocytes-Pluronic F-127 implant expressed FasL and Type II collagen at the 5th week and survived until the 8th week. INTERPRETATION:The result indicates that the expression of FasL by chondrocytes is capable of inducing apoptosis of activated T cells. This suggests a potential role for allogeneic transplantation with chondrocytes.
journal_name
Transpl Immunoljournal_title
Transplant immunologyauthors
Xie GH,Wang SJ,Wang Y,Zhang Y,Zhang HZ,Jin S,Wang QF,Liu ZC,Ge HLdoi
10.1016/j.trim.2008.02.001subject
Has Abstractpub_date
2008-05-01 00:00:00pages
145-51issue
2eissn
0966-3274issn
1878-5492pii
S0966-3274(08)00011-7journal_volume
19pub_type
杂志文章abstract：:Keratinocytes are activated to express MHC class II and ICAM-1 molecules during cutaneous inflammatory reactions. It is controversial how the interaction between these 'nonprofessional' antigen presenting cells (APC) and infiltrating T cells affects the local inflammatory response. To address this issue we analyzed wh...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/0966-3274(93)90059-h
更新日期：1993-01-01 00:00:00
abstract：BACKGROUND:The liver is an immunological organ containing a large number of T, NK and NKT cells, but little is known about intrahepatic immunity after LTx. Here, we investigated whether the distribution of T, NK and CD3(+)CD56(+)NKT cells is altered in transplanted livers under different circumstances. METHODS:Core bi...
journal_title：Transplant immunology
pub_type： 临床试验,杂志文章
doi：10.1016/j.trim.2011.05.006
更新日期：2011-07-01 00:00:00
abstract：:Limiting dilution analysis technique was used to enumerate the circulating precursor frequency of donor and third-party-reactive helper T lymphocytes (HTLpf) in 28 renal allograft recipients before (pre-tx) and at three intervals (T1: 60-90 days, T2: 120-180 days, T3: 360-1620 days) after transplantation (post-tx). Tw...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/s0966-3274(97)80048-2
更新日期：1997-06-01 00:00:00
abstract：:Persistent rejection in the face of treatment and multiple episodes of rejection are associated with the development of chronic rejection and graft loss in solid organ transplantation. The factors that create an environment for rejection that persists in the face of treatment are as yet not understood. The objective o...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2004.11.001
更新日期：2005-03-01 00:00:00
abstract：:We present management strategies utilised for the first case of an urgent live-donor ABO incompatible B blood group renal transplant, in a patient with a prior A blood group lung transplant for cystic fibrosis. Three years on, renal function is excellent and stable, whilst lung function has improved. ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2016.09.002
更新日期：2016-11-01 00:00:00
abstract：:Mixed lymphocyte reaction (MLR) assays were performed serially over 24 months in 19 first cadaver renal transplant recipients. Immunosuppression consisted of cyclosporine, methylprednisolone and azathioprine. Cyclosporine was withdrawn at 6 months postoperatively. The MLR reactivity gradually decreased over the first ...
journal_title：Transplant immunology
pub_type： 临床试验,杂志文章
doi：10.1016/s0966-3274(98)80016-6
更新日期：1998-12-01 00:00:00
abstract：:New immunosuppressive strategies have improved short- and long-term graft survival. The current aim is to decrease the intensity of the immunosuppressive regimen, in an attempt to limit side effects and the direct toxicity of calcineurin inhibitor (CNI) for kidney function. We describe here current experience in liver...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/j.trim.2006.11.007
更新日期：2007-02-01 00:00:00
abstract：:We determined total rabbit-IgG (r-ATG) levels in serum samples before (day 0) and after (day 11 and day 25) unrelated donor umbilical cord blood transplantation (UCBT). Most patients (27/41) suffered from a haematological malignancy. There were 25 children and 16 adults. All patients received rabbit anti-thymocyte glo...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2012.06.003
更新日期：2012-08-01 00:00:00
abstract：:Although the clinical significance of anti-HLA antibodies in heart and lung transplantation is less well studied than in renal transplantation, several studies have shown that heart and lung recipients transplanted in the presence of donor-specific antibodies are at increased risk for early acute rejection and have a ...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/j.trim.2004.01.005
更新日期：2004-06-01 00:00:00
abstract：:Transplant rejection is a multifactorial process involving complex interactions between components of the innate and the acquired immune system. In view of the shortage of donor organs available for transplantation, xenotransplantation of pig organs into man has been considered as a potential solution. However, in com...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/s0966-3274(02)00042-4
更新日期：2002-05-01 00:00:00
abstract：:Adhesion molecule expression is an important event during early transplant failure. The aim of the present study was to examine the release of adhesion molecules during the first minutes of kidney allograft reperfusion in relation to delayed graft function and acute graft rejection. We enrolled 49 renal transplant rec...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2006.08.002
更新日期：2006-11-01 00:00:00
abstract：:Calcineurin inhibitors (CNI) cyclosporine (Csa) and tacrolimus (Tac) are now first intention immunosuppressive drugs in renal transplantation. However, although these treatments are effective for preventing allograft rejection, they are nephrotoxic: they can cause chronic renal dysfunction and degradation of renal gra...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2008.09.002
更新日期：2008-11-01 00:00:00
abstract：:Induction and maintenance immunosuppression protocols with or without long-term steroid therapy in kidney transplant recipients are variable and are transplant center-specific. The aim of this prospective randomized pilot study was to compare 5-year outcomes in kidney recipients maintained on 4 different calcineurin i...
journal_title：Transplant immunology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.trim.2008.08.005
更新日期：2008-11-01 00:00:00
abstract：:Previously, we developed a well-tolerated single-day protocol for induction of stable multilineage chimerism and permanent donor-specific tolerance across major histocompatibility complex (MHC) barriers, with preservation of the host's normal immune responses. In our murine model, recipient mice were treated with a si...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/s0966-3274(98)80039-7
更新日期：1998-09-01 00:00:00
abstract：:Treatment with autologous bone marrow transplantation (ABMT) can change the natural history of diabetes in patients with new-onset Type 1 diabetes (T1D). Effects of syngeneic bone marrow transplantation (syn-BMT) on diabetic nephropathy were studied in streptozotocin-induced diabetic mice. Diabetic mice received sibli...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2009.12.003
更新日期：2010-02-01 00:00:00
abstract：BACKGROUND:Donor specific hematopoietic cell transplantation has long been recognized for its potential in tolerance induction for subsequently transplanted organs. We have recently published that co-administration of Myeloid Progenitor (MP) and third party Hematopoietic Stem Cells (HSC) can induce MP-specific toleranc...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2014.04.001
更新日期：2014-08-01 00:00:00
abstract：BACKGROUND:Antibody-mediated rejection (ABMR) is the leading cause of kidney graft loss worldwide. Criteria for acute humoral rejection (currently labeled active humoral rejection) established by the 2007 Banff classification are highly specific but lack sensitivity. Modifications to the Banff classification were intro...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2018.08.008
更新日期：2018-12-01 00:00:00
abstract：INTRODUCTION:Glutathione (GSH) is added to University of Wisconsin (UW) organ preservation solution to protect against oxidative stress. This study assesses the effect of GSH-supplementation on endothelial function in tissues subjected to cold ischaemia and compares its effects to a mono-ethyl ester equivalent (GSH-MEE...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2007.06.002
更新日期：2007-11-01 00:00:00
abstract：BACKGROUND:While acute neurotoxic side effects of calcineurin inhibitors (CNI) are well-known, data upon long-term effects on brain structure and function are sparse. We hypothesize that long-term CNI therapy affects the neuroimmune system, thereby, increasing the risk of neurodegeneration. Here, we measured the impact...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2019.101248
更新日期：2020-02-01 00:00:00
abstract：:We investigated the dynamics of the CD4+ and CD8+ lymphocyte subsets, and the expression of activation markers in cardiac transplant recipients. We tested 132 peripheral blood samples from 62 cardiac transplant recipients using fluorescent staining and flow cytometry analysis. The results were correlated with histolog...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/s0966-3274(02)00072-2
更新日期：2002-11-01 00:00:00
abstract：:Rituximab is frequently used in the setting of ABO-incompatible renal transplants, and highly sensitized patients. Its interference with B-cell flow cytometric crossmatch (B-FCXM) is well known. However, its effect on the T-cell flow cytometric crossmatch (T-FCXM) has not been described. We aimed to evaluate the effec...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2020.101360
更新日期：2021-02-01 00:00:00
abstract：:The ImmuKnow assay measures cell-mediated immunity, quantifying ATP production from peripheral blood CD4+T-cells in solid-organ transplant patients who undergo immunosuppressive therapy. We aimed to measure functional immunity in lung transplant recipients and correlate Immuknow values with immunosuppression levels, p...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2016.04.001
更新日期：2016-07-01 00:00:00
abstract：:Despite improvements in the prevention and treatment of graft-versus-host disease (GvHD) this allogeneic immune response is still one of major complications following allogeneic stem cell transplantation (SCT). Identification of patients at risk for the development of acute and chronic GvHD would facilitate early inte...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2012.07.003
更新日期：2012-12-01 00:00:00
abstract：:National registry data indicate a trend towards the incorporation of lymphocyte depletion antibody induction therapy into immunosuppressive regimens for solid organ transplantation. Depletional induction has been shown to reduce the risk of early acute rejection, but increase the risk of immune incompetence. As such, ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2008.09.003
更新日期：2008-11-01 00:00:00
abstract：:Administration of anti-D immunoglobulin to D- women after delivery of a D+ infant has dramatically reduced the number of immunised women and cases of haemolytic disease of the fetus and newborn. The use of monoclonal anti-D might alleviate some of the pressures on maintaining adequate supplies of plasma sourced anti-D...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/s0966-3274(02)00066-7
更新日期：2002-08-01 00:00:00
abstract：BACKGROUND:Response to Hepatitis B virus (HBV) vaccination can be diminished in some (50-80%) but not all dialysis patients. We hypothesized, that the response to vaccination on dialysis may correlate with the development of anti-HLA antibodies after renal transplantation and might therefore be a valuable parameter to ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2017.04.003
更新日期：2017-06-01 00:00:00
abstract：BACKGROUND:Cytomegalovirus (CMV)-specific T-cells are crucial to prevent CMV disease. CMV seropositive recipients transplanted with stem cells from a CMV seronegative allogeneic donor (R+D-) may be at risk for CMV disease due to absence of donor CMV-specific memory T-cells in the graft. METHODS:We analyzed the duratio...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2018.04.003
更新日期：2018-08-01 00:00:00
abstract：:The mechanisms by which alloreactive memory T-cells are generated in non-sensitized individuals have begun to be elucidated. It is generally accepted that a very high level of crossreactivity is an essential feature of the T-cell receptor. Indeed it has recently been shown that alloreactivity from viral specific memor...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/j.trim.2010.06.008
更新日期：2010-08-01 00:00:00
abstract：:Data binding the expression of Toll-like 4 receptor (TLR4ex), transplanted kidney function, and the cause of pre-transplant end-stage renal disease are scarcely available. OBJECTIVE:To investigate the relationship between pre-transplant chronic interstitial nephritis (CIN), TLR4ex and transplanted kidney function. M...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2020.101319
更新日期：2020-10-01 00:00:00
abstract：BACKGROUND:We tested the hypothesis that the best time for genetic modification is while the cell viability of the graft is reduced for long-term preservation. The hemagglutinating virus of Japan (HVJ)-liposome method, a nonviral gene transfer technique, was used with a luciferase gene to test the efficacy of protein i...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/S0966-3274(03)00002-9
更新日期：2003-01-01 00:00:00
