Abstract:
:We studied the non-specific interactions of two azo compounds: biebrich scarlet (BS) and naphthochrome green (NG), with four model proteins: bovine serum albumin, ovalbumin, poly-L-lysine and hemoglobin by UV-VIS spectrometry, fluorophotometry and circular dichroism melting technique. The optimal acidities of NG and BS for binding to proteins correspond to the physiological pHs of skin and gastro tissues. The saturation binding numbers of BS and NG on peptide chains were determined and the effects of electrolytes and temperature were investigated. These interactions were fitted by the Temkin absorption model and their thermodynamic parameters were calculated. The different bindings of BS and NG to proteins were compared from their molecular structures. We inferred that an ion-pair electrostatic interaction first fixes azo compounds to basic amino acid residues and subsequent binding involves the collective action of other non-covalent bonds: hydrogen bond, van der Waals force, and hydrophobic interaction. This combination of bonds caused a change of secondary conformation of protein from beta-sheet to helix and the possible process was illustrated. The potential protein toxicity resulting from such a non-specific binding was analyzed. Besides, the interaction of BS with peptide chains was applied to protein assay.
journal_name
Amino Acidsjournal_title
Amino acidsauthors
Gao HW,Liu XH,Qiu Z,Tan Ldoi
10.1007/s00726-008-0058-1subject
Has Abstractpub_date
2009-02-01 00:00:00pages
251-60issue
2eissn
0939-4451issn
1438-2199journal_volume
36pub_type
杂志文章相关文献
AMINO ACIDS文献大全abstract::Glioma is the most common type of brain tumor, and has the worst prognosis in human malignancy. Experimental evidence suggests that the use of high concentrations of various amino acids may perturb neoplastic cell growth. Thus, the aim of this study was to investigate whether essential amino acids can alter the growth...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1122-9
更新日期:2012-08-01 00:00:00
abstract::In this study, we investigated the activating role of Mcm1p at ARG1 during arginine starvation. Our results showed that two Mcm1p binding sites positively contribute to ARG1 transcription and cell growth. Especially, we provide strong evidence that the Mcm1p binding sites play a positive role in ARG1 transcription to ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0687-z
更新日期:2011-02-01 00:00:00
abstract::Extracellular peptide ligand binding sites, which bind the N-termini of angiotensin II (AngII) and bradykinin (BK) peptides, are located on the N-terminal and extracellular loop 3 regions of the AT(1)R and BKRB(1) or BKRB(2) G-protein-coupled receptors (GPCRs). Here we synthesized peptides P15 and P13 corresponding to...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1405-9
更新日期:2013-03-01 00:00:00
abstract::Early evaluation of cancer response to a therapeutic regimen can help increase the effectiveness of treatment schemes and, by enabling early termination of ineffective treatments, minimize toxicity, and reduce expenses. Biomarkers that provide early indication of tumor therapy response are urgently needed. Solid tumor...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0548-9
更新日期:2011-11-01 00:00:00
abstract::The glutamate/cystine antiporter system x(c)(-) transports cystine into cells in exchange for glutamate at a ratio of 1:1. It is composed of a specific light chain, xCT, and a heavy chain, 4F2, linked by a disulfide bridge. Intracellularly, cystine is reduced into cysteine, the rate-limiting precursor of glutathione (...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-011-0862-x
更新日期:2012-01-01 00:00:00
abstract::Several descriptors of protein structure at the sequence and residue levels have been recently proposed. They are widely adopted in the analysis and prediction of structural and functional characteristics of proteins. Numerous in silico methods have been developed for sequence-based prediction of these descriptors. Ho...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0721-1
更新日期:2011-03-01 00:00:00
abstract::Several new beta,beta-dihalo and beta-halo-beta-substituted dehydroalanines and dehydrodipeptides were synthesized by reacting the corresponding dehydroamino acid derivative with a N-halosuccinimide or in the case of beta,beta-di-iododehydroalanines with iodine. The results obtained confirmed that the stereochemical o...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0466-x
更新日期:2010-07-01 00:00:00
abstract::This article deals with the synthesis in solid phase and characterization of a nucleoamino amide, based on a phenylalaninamide moiety which was N-conjugated to a thymine nucleobase. In analogy to the natural nucleobase-amino acid conjugates, endowed with a wide range of biological properties, the nucleoamino amide int...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-018-2562-2
更新日期:2018-07-01 00:00:00
abstract::The G protein-coupled receptor kinase (GRK) family consists of seven cytosolic serine/threonine (Ser/Thr) protein kinases, and among them, GRK2 is involved in the regulation of an enormous range of both G protein-coupled receptors (GPCRs) and non-GPCR substrates that participate in or regulate many critical cellular p...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-020-02864-x
更新日期:2020-07-01 00:00:00
abstract::Starting from a chiral 4-hydroxymethyl pyrrolidin-2-one, an isostere of α-methyl homoserine tethered on a γ-lactam ring was prepared exploiting a stereoselective acylation-methylation sequence, followed by Curtius rearrangement, and structural assignment was confirmed by n.O.e. experiments. By reverting the sequence, ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-014-1671-9
更新日期:2014-04-01 00:00:00
abstract::The lipidicα-amino acids (LAAs) are non-naturalα-amino acids with saturated or unsaturated long aliphatic side chains. LAAs and their derivatives (lipid mimetics) together with the lipidic peptides represent a class of compounds which combine structural features of lipids with those of amino acids and peptides. Racemi...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00807940
更新日期:1996-09-01 00:00:00
abstract::Lamin A/C proteins are the major components of a thin proteinaceous filamentous meshwork, the lamina, that underlies the inner nuclear membrane. A few specific mutations in the lamin A/C gene cause a disease with remarkably different clinical features: FPLD, or familial partial lipodystrophy (Dunnigan-type), which mai...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1108-7
更新日期:2012-08-01 00:00:00
abstract::Polyamines have been related to the "Crassulacean Acid Metabolism" (CAM) in higher plants. Such relationship was however observed in plants where CAM activity is inducible by external factors. Results presented here indicate that, inOpuntia F. indica, cladodes where onset of CAM is dependent on internal conditions, i....
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00814160
更新日期:1994-06-01 00:00:00
abstract::Some of the chemistry of amino acids going on in our laboratory (Laboratoire des Amino acides Peptides et Protéines) is described as well as some mass spectrometry methodology for their characterization particularly on solid supports. Several aspects are presented including: (i) the stereoselective synthesis of natura...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/BF01388176
更新日期:1999-01-01 00:00:00
abstract::D-Asp-containing proteins have been implicated in many aging-related diseases. To clarify the role of D-Asp-containing proteins in such diseases, we developed a screening system for these proteins using a D-aspartyl endopeptidase that specifically cleaves the proteins at the C-terminus. The digested proteins were dete...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-008-0040-y
更新日期:2009-01-01 00:00:00
abstract::Creatine is an amino acid that has a pivotal role in energy metabolism of cells. Creatine acts as an "ATP shuttle", carrying ATP to the sites where it is utilized, through its reversible phosphorylation by creatine kinase. Moreover, the creatine-phosphocreatine system delays ATP depletion during anoxia or ischemia, th...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-013-1525-x
更新日期:2013-10-01 00:00:00
abstract::Transglutaminases are confounding enzymes which are known to play key roles in various cellular processes. In this paper, we aim to bring together several pieces of evidence from published research and literature that suggest a potentially vital role for transglutaminases in receptor tyrosine kinases (RTK) signalling....
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-011-1113-x
更新日期:2013-01-01 00:00:00
abstract::The dipeptide carnosine is found in high concentrations in human skeletal muscle and shows large inter-individual differences. Sex and age are determining factors, however, systematic studies investigating the sex effects on muscle carnosine content throughout the human lifespan are lacking. Despite the large inter-in...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1197-3
更新日期:2012-07-01 00:00:00
abstract::Recovery of amino acid (AA) metabolism and the associated clinical implications in chronic hepatitis C (CHC) patients with sustained virological response (SVR) following anti-hepatitis C virus (HCV) therapy remains elusive. A prospective cohort study was conducted on 222 CHC patients with SVR. Eighty-two age-matched m...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-016-2360-7
更新日期:2017-02-01 00:00:00
abstract::How to enhance fruit tolerance to cold stress is an important biological interest. In this paper, we found that mango (Mangifera indica L.) fruit treated with 10 μM brassinolide (BL) showed a higher tolerance to cold temperature of 5 °C. Further, we compared the changes in expression profiles of plasma membrane (PM) p...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1327-6
更新日期:2012-12-01 00:00:00
abstract::The purpose of the present study was to investigate the relationship between plasma carnitine concentration and body composition variation in relation to muscular and fat masses since there is no experimentally proved correlation between plasma carnitine and body masses. We used bioelectric impedance analysis (BIA), t...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF01318855
更新日期:1998-01-01 00:00:00
abstract::Reports on nucleobase-containing chiral peptides (both natural and artificial) and achiral pseudopeptides are reviewed. Their synthesis, structural features, DNA and RNA-binding ability, as well as some other interesting applications which make them promising diagnostic/therapeutic agents of great importance in many a...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-010-0567-6
更新日期:2010-06-01 00:00:00
abstract::In this paper we report the complete separation of amino acids as DABS-derivatives using a 3µm Supelcosil LC-18 (25 cm × 2.1 mm I.D.) narrowbore column. The system described makes it possible to perform the analysis of DABS-amino acids with a sensitivity to the femtomole level. We have also studied the conditions nece...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00806005
更新日期:1992-10-01 00:00:00
abstract::The study was designed to investigate the effects of NMDA receptor agonist on the behavioral activity in rats with experimental hyperammonemia. The experiments were performed on adult male Wistar rats. Experimental hyperammonemia was induced by intraperitoneal injections of tioacetamide (TAA, 200 mg/kg) for three cons...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-004-0122-4
更新日期:2005-02-01 00:00:00
abstract::Chlamydocin is a powerfulin vitro antitumoral agent, quickly inactivatedin vivo. A series of cyclic tetrapeptides related to chlamydocin or HC toxin and bearing a bioactive alkylating group on anε-amino-lysyl function have been examined for their antitumoral activity on L1210 and P388 murine leukemia cell lines. One a...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00813752
更新日期:1994-10-01 00:00:00
abstract::Arginine (Arg) and glutamine (Gln) utilization is greatly increased during catabolic stress. While the supply of both amino acids has been advocated in this situation, arginine administration is possibly associated with deleterious effects. From a metabolic point of view, these two amino acids are reciprocal precursor...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0601-8
更新日期:2010-11-01 00:00:00
abstract::Cupiennin 1a, a cytolytic peptide isolated from the venom of the spider Cupiennius salei, exhibits broad membranolytic activity towards bacteria, trypanosomes, and plasmodia, as well as human blood and cancer cells. In analysing the cytolytic activity of synthesised all-D: - and all-L: -cupiennin 1a towards pro- and e...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0471-0
更新日期:2011-01-01 00:00:00
abstract::The human organism is exposed to numerous processes that generate reactive oxygen species (ROS). ROS may directly or indirectly cause oxidative modification and damage of proteins. Protein oxidation is regarded as a crucial event in the pathogenesis of various diseases ranging from rheumatoid arthritis to Alzheimer's ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-005-0203-z
更新日期:2005-12-01 00:00:00
abstract::Green tea which is widely consumed in China, Japan and India, contains polyphenolic compounds, which account for 30% of the dry weight of the leaves. Most of the polyphenols are flavanols, of which (-)-epigallocatechin-3-gallate (EGCG) is most abundant. Epidemiological studies revealed that the incidences of stomach a...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s726-002-8197-9
更新日期:2002-01-01 00:00:00
abstract::Metformin, the drug of first choice in type 2 diabetes mellitus (T2DM), reduces cardiovascular (CV) morbidity and mortality in part independently of improved glycemic control and changes in traditional risk factors. However, there are discordant reports on the effects of metformin on endothelial function in T2DM. Our ...
journal_title:Amino acids
pub_type: 临床试验,杂志文章
doi:10.1007/s00726-015-1976-3
更新日期:2015-09-01 00:00:00