Phage display peptide probes for imaging early response to bevacizumab treatment.

Abstract:

:Early evaluation of cancer response to a therapeutic regimen can help increase the effectiveness of treatment schemes and, by enabling early termination of ineffective treatments, minimize toxicity, and reduce expenses. Biomarkers that provide early indication of tumor therapy response are urgently needed. Solid tumors require blood vessels for growth, and new anti-angiogenic agents can act by preventing the development of a suitable blood supply to sustain tumor growth. The purpose of this study is to develop a class of novel molecular imaging probes that will predict tumor early response to an anti-angiogenic regimen with the humanized vascular endothelial growth factor antibody bevacizumab. Using a bevacizumab-sensitive LS174T colorectal cancer model and a 12-mer bacteriophage (phage) display peptide library, a bevacizumab-responsive peptide (BRP) was identified after six rounds of biopanning and tested in vitro and in vivo. This 12-mer peptide was metabolically stable and had low toxicity to both endothelial cells and tumor cells. Near-infrared dye IRDye800-labeled BRP phage showed strong binding to bevacizumab-treated tumors, but not to untreated control LS174T tumors. In addition, both IRDye800- and (18)F-labeled BRP peptide had significantly higher uptake in tumors treated with bevacizumab than in controls treated with phosphate-buffered saline. Ex vivo histopathology confirmed the specificity of the BRP peptide to bevacizumab-treated tumor vasculature. In summary, a novel 12-mer peptide BRP selected using phage display techniques allowed non-invasive visualization of early responses to anti-angiogenic treatment. Suitably labeled BRP peptide may be potentially useful pre-clinically and clinically for monitoring treatment response.

journal_name

Amino Acids

journal_title

Amino acids

authors

Cao Q,Liu S,Niu G,Chen K,Yan Y,Liu Z,Chen X

doi

10.1007/s00726-010-0548-9

subject

Has Abstract

pub_date

2011-11-01 00:00:00

pages

1103-12

issue

5

eissn

0939-4451

issn

1438-2199

journal_volume

41

pub_type

杂志文章
  • Expression and function of arginine-producing and consuming-enzymes in the kidney.

    abstract::The kidney plays a key role in arginine metabolism. Arginine production is controlled by argininosuccinate synthetase (ASS) and argininosuccinate lyase (ASL) which metabolize citrulline and aspartate to arginine and fumarate whereas arginine consumption is dependent on arginine:glycine amidinotransferase (GAT), which ...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s00726-011-0897-z

    authors: Levillain O

    更新日期:2012-04-01 00:00:00

  • Easy preparation of dehydroalanine building blocks equipped with oxazolidin-2-one chiral auxiliaries, and applications to the stereoselective synthesis of substituted tryptophans.

    abstract::Chiral dehydroamino acid building blocks are versatile starting materials for the preparation of optically active unusual amino acids and other compounds of pharmacological interest. Herein we disclose the expedient preparation of dehydroalanines (ΔAla) equipped with oxazolidin-2-one (Oxd) chiral auxiliaries, Ts-Oxd-Δ...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s00726-014-1839-3

    authors: De Marco R,Cavina L,Greco A,Gentilucci L

    更新日期:2014-12-01 00:00:00

  • Asymmetric hydrogenation of dehydrodipeptides bearing different protecting groups.

    abstract::N-[(Z)-N-Benzoyl- or N-boc-(2-fluorophenyl)dehydroalanyl]-(R)- or (S)-phenyl-alanines 1,2,5 and 6 were hydrogenated in the presence of chiral and achiral rhodium complexes. The optical induction is compared to the results obtained using the corresponding esters as substrates. ...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s007260200018

    authors: Döbler C,Kreuzfeld HJ,Fischer C,Michalik M

    更新日期:2002-06-01 00:00:00

  • The postprandial use of dietary amino acids as an energy substrate is delayed after the deamination process in rats adapted for 2 weeks to a high protein diet.

    abstract::The aim of this study was to determine the contribution of dietary amino acids (AA) to energy metabolism under high protein (HP) diets, using a double tracer method to follow simultaneously the metabolic fate of α-amino groups and carbon skeletons. Sixty-seven male Wistar rats were fed a normal (NP) or HP diet for 14 ...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s00726-010-0756-3

    authors: Fromentin C,Azzout-Marniche D,Tomé D,Even P,Luengo C,Piedcoq J,Fromentin G,Gaudichon C

    更新日期:2011-05-01 00:00:00

  • Stabilization of Angiotensin-(1-7) by key substitution with a cyclic non-natural amino acid.

    abstract::Angiotensin-(1-7) [Ang-(1-7)], a heptapeptide hormone of the renin-angiotensin-aldosterone system, is a promising candidate as a treatment for cancer that reflects its anti-proliferative and anti-angiogenic properties. However, the peptide's therapeutic potential is limited by the short half-life and low bioavailabili...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s00726-017-2471-9

    authors: Wester A,Devocelle M,Tallant EA,Chappell MC,Gallagher PE,Paradisi F

    更新日期:2017-10-01 00:00:00

  • Putative occurrence of lysine decarboxylase isoforms in soybean (Glycine max) seedlings.

    abstract::The activity of lysine decarboxylase was studied in 3-day-old soybean (Glycine max (L.) Meer cv. Sakai) seedlings also in relation to light conditions. Lysine decarboxylase activity was mainly localized in the roots and to a lesser extent in the hypocotyls and was detectable in both the soluble and particulate fractio...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s00726-008-0029-6

    authors: Ohe M,Scoccianti V,Bagni N,Tassoni A,Matsuzaki S

    更新日期:2009-01-01 00:00:00

  • Cytotoxicity of boron containing dipeptide analogs.

    abstract::This work is an extension of our previous work (Hall et al., 1993) on the synthesis and cytotoxic activity of boronated peptides. The aim of this work was to carry out structural modifications of the amine terminal in compounds1 and2, to increase water solubility, and its effect on the cytotoxicity to tumor cell lines...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/BF00806550

    authors: Karthikeyan S,Sood A,Tomasz J,Spielvogel BF,Hall IH

    更新日期:1995-12-01 00:00:00

  • Age-related salivary polyamine increase in adolescents wearing orthodontic Ni-Ti archwires.

    abstract::Until now information about the influence of puberty on gingival tissue responses to Ni-Ti alloy haven't been available. Since our previous researches have demonstrated that Ni-Ti appliances have an influence on hyperplastic gingivopathy and data has pointed out a possible hormonal influence on the susceptibility of g...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s007260200001

    authors: Venza M,Visalli M,Ruggeri P,Cicciù D,Teti D

    更新日期:2002-01-01 00:00:00

  • Recovery of pan-genotypic and genotype-specific amino acid alterations in chronic hepatitis C after viral clearance: transition at the crossroad of metabolism and immunity.

    abstract::Recovery of amino acid (AA) metabolism and the associated clinical implications in chronic hepatitis C (CHC) patients with sustained virological response (SVR) following anti-hepatitis C virus (HCV) therapy remains elusive. A prospective cohort study was conducted on 222 CHC patients with SVR. Eighty-two age-matched m...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s00726-016-2360-7

    authors: Chang ML,Cheng ML,Chang SW,Tang HY,Chiu CT,Yeh CT,Shiao MS

    更新日期:2017-02-01 00:00:00

  • Pathways involved in alanyl-glutamine-induced changes in neutrophil amino- and alpha-keto acid homeostasis or immunocompetence.

    abstract::We examined the effects of DON [glutamine-analogue and inhibitor of glutamine-requiring enzymes], alanyl-glutamine (regarding its role in neutrophil immunonutrition) and alanyl-glutamine combined with L-NAME, SNAP, DON, beta-alanine and DFMO on neutrophil amino and alpha-keto acid concentrations or important neutrophi...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s00726-006-0395-x

    authors: Mühling J,Burchert D,Langefeld TW,Matejec R,Harbach H,Engel J,Wolff M,Welters ID,Fuchs M,Menges T,Krüll M,Hempelmann G

    更新日期:2007-09-01 00:00:00

  • The role of dietary creatine.

    abstract::The daily requirement of a 70-kg male for creatine is about 2 g; up to half of this may be obtained from a typical omnivorous diet, with the remainder being synthesized in the body Creatine is a carninutrient, which means that it is only available to adults via animal foodstuffs, principally skeletal muscle, or via su...

    journal_title:Amino acids

    pub_type: 杂志文章,评审

    doi:10.1007/s00726-016-2188-1

    authors: Brosnan ME,Brosnan JT

    更新日期:2016-08-01 00:00:00

  • Kinetics of biomarkers: biological and technical validity of isoprostanes in plasma.

    abstract::Isoprostanes, non-enzymatic peroxidation products of arachidonic acid, are attractive biomarkers of oxidative stress in research in biology, medicine and nutrition. For the appropriate use of biomarkers it is required that these are both biologically and technically valid. Whereas the biological validity of isoprostan...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s00726-005-0229-2

    authors: Coolen SA,van Buuren B,Duchateau G,Upritchard J,Verhagen H

    更新日期:2005-12-01 00:00:00

  • Biochemical characterization of the catecholaldehyde reactivity of L-carnosine and its therapeutic potential in human myocardium.

    abstract::Oxidative deamination of norepinephrine (NE) and dopamine (DA) by monoamine oxidase (MAO) generates the catecholaldehydes 3,4-dihydroxyphenylglycolaldehyde (DOPEGAL) and 3,4-dihydroxyphenylacetaldehyde (DOPAL), respectively, and H2O2. Catecholaldehydes are highly reactive electrophiles that have been implicated as cau...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s00726-018-2647-y

    authors: Nelson MM,Builta ZJ,Monroe TB,Doorn JA,Anderson EJ

    更新日期:2019-01-01 00:00:00

  • Insilico study of the A(2A)R-D (2)R kinetics and interfacial contact surface for heteromerization.

    abstract::G-protein-coupled receptors (GPCRs) are cell surface receptors. The dynamic property of receptor-receptor interactions in GPCRs modulates the kinetics of G-protein signaling and stability. In the present work, the structural and dynamic study of A(2A)R-D(2)R interactions was carried to acquire the understanding of the...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s00726-012-1218-x

    authors: Prakash A,Luthra PM

    更新日期:2012-10-01 00:00:00

  • Study on the decrease of renal D-amino acid oxidase activity in the rat after renal ischemia by chiral ligand exchange capillary electrophoresis.

    abstract::D: -Amino acid oxidase (DAAO) in mammal kidney regulates the renal reactive oxygen species (ROS) levels directly and plays a leading role in the development of ROS-mediated renal pathologic damages based on its crucial role in the oxidative deamination of D: -amino acids and the consequent generation of H(2)O(2). Quan...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s00726-010-0811-0

    authors: Zhang H,Qi L,Lin Y,Mao L,Chen Y

    更新日期:2012-01-01 00:00:00

  • L-Arginine stimulates proliferation and prevents endotoxin-induced death of intestinal cells.

    abstract::This study tested the hypothesis that L-arginine (Arg) may stimulate cell proliferation and prevent lipopolysaccharide (LPS)-induced death of intestinal cells. Intestinal porcine epithelial cells (IPEC-1) were cultured for 4 days in Arg-free Dulbecco's modified Eagle's-F12 Ham medium (DMEM-F12) containing 10, 100 or 3...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s00726-009-0334-8

    authors: Tan B,Yin Y,Kong X,Li P,Li X,Gao H,Li X,Huang R,Wu G

    更新日期:2010-04-01 00:00:00

  • SILAC-based proteomic analysis to dissect the "histone modification signature" of human breast cancer cells.

    abstract::In living cells, the N-terminal tails of core histones, the proteinaceous component of nucleosomes, are subjected to a range of covalent post-translational modifications (PTMs), which have specific roles in modulating chromatin structure and function. A growing body of evidence suggests that deregulation of histone mo...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s00726-010-0668-2

    authors: Cuomo A,Moretti S,Minucci S,Bonaldi T

    更新日期:2011-07-01 00:00:00

  • Differential associations of circulating asymmetric dimethylarginine and cell adhesion molecules with metformin use in patients with type 2 diabetes mellitus and stable coronary artery disease.

    abstract::Metformin, the drug of first choice in type 2 diabetes mellitus (T2DM), reduces cardiovascular (CV) morbidity and mortality in part independently of improved glycemic control and changes in traditional risk factors. However, there are discordant reports on the effects of metformin on endothelial function in T2DM. Our ...

    journal_title:Amino acids

    pub_type: 临床试验,杂志文章

    doi:10.1007/s00726-015-1976-3

    authors: Kruszelnicka O,Chyrchel B,Golay A,Surdacki A

    更新日期:2015-09-01 00:00:00

  • Asymmetric synthesis of α-amino acids via homologation of Ni(II) complexes of glycine Schiff bases. Part 3: Michael addition reactions and miscellaneous transformations.

    abstract::The major goal of this review is a critical discussion of the literature data on asymmetric synthesis of α-amino acids via Michael addition reactions involving Ni(II)-complexes of amino acids. The material covered is divided into two conceptually different groups dealing with applications of: (a) Ni(II)-complexes of g...

    journal_title:Amino acids

    pub_type: 杂志文章,评审

    doi:10.1007/s00726-014-1764-5

    authors: Aceña JL,Sorochinsky AE,Soloshonok V

    更新日期:2014-09-01 00:00:00

  • Properties of putrescine uptake by PotFGHI and PuuP and their physiological significance in Escherichia coli.

    abstract::Properties of putrescine uptake by PotFGHI and PuuP and their physiological significance were studied using a polyamine biosynthesis and uptake deficient Escherichia coli KK3131 transformed with pACYC184 containing potFGHI or puuP. Putrescine uptake activity of E. coli KK3131 transformed with pACYC184-PotFGHI was high...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s00726-013-1517-x

    authors: Terui Y,Saroj SD,Sakamoto A,Yoshida T,Higashi K,Kurihara S,Suzuki H,Toida T,Kashiwagi K,Igarashi K

    更新日期:2014-03-01 00:00:00

  • On the segregation of protein ionic residues by charge type.

    abstract::Based on ubiquitous presence of large ionic motifs and clusters in proteins involved in gene transcription and protein synthesis, we analyzed the distribution of ionizable sidechains in a broad selection of proteins with regulatory, metabolic, structural and adhesive functions, in agonist, antagonist, toxin and antimi...

    journal_title:Amino acids

    pub_type: 杂志文章,评审

    doi:10.1007/s00726-012-1418-4

    authors: Parker MS,Balasubramaniam A,Parker SL

    更新日期:2012-12-01 00:00:00

  • In silico investigation of molecular mechanism of laminopathy caused by a point mutation (R482W) in lamin A/C protein.

    abstract::Lamin A/C proteins are the major components of a thin proteinaceous filamentous meshwork, the lamina, that underlies the inner nuclear membrane. A few specific mutations in the lamin A/C gene cause a disease with remarkably different clinical features: FPLD, or familial partial lipodystrophy (Dunnigan-type), which mai...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s00726-011-1108-7

    authors: Rajendran V,Purohit R,Sethumadhavan R

    更新日期:2012-08-01 00:00:00

  • Taurine in adipocytes prevents insulin-mediated H2O2 generation and activates Pka and lipolysis.

    abstract::Among many actions assigned to taurine (Tau), the most abundant amino acid in numerous mammalian tissues, it prevents high-fat diet-induced obesity with increasing resting energy expenditure. To sustain this Tau action, the goal of the present study was to explore the acute effects of Tau on baseline and on adrenaline...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s00726-011-0919-x

    authors: Piña-Zentella G,de la Rosa-Cuevas G,Vázquez-Meza H,Piña E,de Piña MZ

    更新日期:2012-05-01 00:00:00

  • Kinetics of taurine depletion and repletion in plasma, serum, whole blood and skeletal muscle in cats.

    abstract::The relationship between taurine concentrations of plasma, whole blood, serum and skeletal muscle during taurine depletion and repletion was investigated in cats, to identify the most useful indicators of taurine status. Sixteen cats were fed a purified diet containing either 0 or 0.15 g/kg taurine for 5 months. Treat...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s007260170006

    authors: Pacioretty L,Hickman MA,Morris JG,Rogers QR

    更新日期:2001-12-01 00:00:00

  • Analysis of the Plasmodium falciparum proteasome using Blue Native PAGE and label-free quantitative mass spectrometry.

    abstract::Detailed knowledge of the composition of protein complexes is crucial for the understanding of their structure and function; however, appropriate techniques for compositional analyses of complexes largely rely on elaborate tagging, immunoprecipitation, cross-linking and purification strategies. The proteasome is a pro...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s00726-012-1296-9

    authors: Sessler N,Krug K,Nordheim A,Mordmüller B,Macek B

    更新日期:2012-09-01 00:00:00

  • Glutathione and its associated enzymes in peripheral blood cells in different stages of chronic renal insufficiency.

    abstract::Reduced glutathione (GSH) levels and glutathione reductase (GR) and glutathione S-transferase (GST) activities were investigated in the erythrocytes and lymphocytes of non-dialyzed patients with varying degrees of chronic renal insufficiency, and also of patients on regular hemodialysis treatment. GSH, GR and GST leve...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/BF00805943

    authors: Mimic-Oka J,Simic T,Djukanovic L,Stefanovski J,Ramic Z

    更新日期:1992-10-01 00:00:00

  • The plant extracellular transglutaminase: what mammal analogues tell.

    abstract::The extracellular transglutaminases (TGs) in eukaryotes are responsible for the post-translational modification of proteins through different reactions, cross-linking being the best known. In higher plants, extracellular TG appears to be involved in roles similar to those performed by the mammalian counterparties. Sin...

    journal_title:Amino acids

    pub_type: 杂志文章,评审

    doi:10.1007/s00726-013-1605-y

    authors: Del Duca S,Verderio E,Serafini-Fracassini D,Iorio R,Cai G

    更新日期:2014-03-01 00:00:00

  • Chemically functionalized single-walled carbon nanotubes enhance the glutamate uptake characteristics of mouse cortical astrocytes.

    abstract::Using a radioactive glutamate uptake assay and immunolabeling, we report that single-walled carbon nanotubes, chemically functionalized with polyethylene glycol (SWCNT-PEG), delivered as a colloidal solute, cause an increase in the uptake of extracellular glutamate by astrocytes and an increase in the immunoreactivity...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s00726-015-1970-9

    authors: Gottipati MK,Bekyarova E,Haddon RC,Parpura V

    更新日期:2015-07-01 00:00:00

  • Ca2+ -independent effects of spermine on pyruvate dehydrogenase complex activity in energized rat liver mitochondria incubated in the absence of exogenous Ca2+ and Mg2+.

    abstract::In the absence of exogenous Ca(2+) and Mg(2+) and in the presence of EGTA, which favours the release of endogenous Ca(2+), the polyamine spermine is able to stimulate the activity of pyruvate dehydrogenase complex (PDC) of energized rat liver mitochondria (RLM). This stimulation exhibits a gradual concentration-depend...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s00726-008-0099-5

    authors: Pezzato E,Battaglia V,Brunati AM,Agostinelli E,Toninello A

    更新日期:2009-03-01 00:00:00

  • A novel method for promoting heterologous protein expression in Escherichia coli by fusion with the HIV-1 TAT core domain.

    abstract::The human immunodeficiency virus type 1 (HIV-1) transactivator of transcription (TAT) protein, a member of the protein transduction domain (PTD) superfamily, can deliver heterologous proteins across most biomembranes without losing bioactivity. However, there is no report on whether the TAT core domain containing the ...

    journal_title:Amino acids

    pub_type: 杂志文章

    doi:10.1007/s00726-010-0534-2

    authors: Wu Y,Ren C,Gao Y,Hou B,Chen T,Zhang C

    更新日期:2010-08-01 00:00:00