Abstract:
:In this paper we report the complete separation of amino acids as DABS-derivatives using a 3µm Supelcosil LC-18 (25 cm × 2.1 mm I.D.) narrowbore column. The system described makes it possible to perform the analysis of DABS-amino acids with a sensitivity to the femtomole level. We have also studied the conditions necessary for using the narrow-bore columns for routine analysis, paying particular attention to the problem of providing adequate protection for the analytical column. We have found it very suitable to use a (2 cm × 2.1 mm I.D.) guard column filled with a 40µm Pelliguard LC-18, pellicular packing resin, without affecting the complete resolution of the DABS-amino acids. Comparing the results obtained using conventional HPLC columns (3-5µm Supelcosil LC-18) of different lengths (15 and 25 cm × 4.6 mm I.D.) with those obtainable with the narrow-bore columns used in this work, it is possible to achieve a much greater sensitivity using the narrow-bore columns. In short, using the appropriate guard column and the "standard" HPLC apparatus used, the narrow-bore columns are very useful for routine analyses of DABS-amino acids with a sensitivity at the femtomole level.
journal_name
Amino Acidsjournal_title
Amino acidsauthors
Stocchi V,Palma F,Piccoli G,Biagiarelli B,Magnani M,Masat L,Cucchiarini Ldoi
10.1007/BF00806005subject
Has Abstractpub_date
1992-10-01 00:00:00pages
303-9issue
3eissn
0939-4451issn
1438-2199journal_volume
3pub_type
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