Abstract:
:A growing body of evidence implicates endoplasmic reticulum (ER)-induced cellular dysfunction and apoptosis as important factors to a variety of diseases. In endothelial cells (ECs), the sulfur-containing amino acid homocysteine (Hcy) causes EC apoptosis and reactive oxygen species (ROS) generation through induction of ER stress. Here, we have investigated whether piceatannol (Pic), a resveratrol analog, could protect ECs against Hcy-induced apoptosis, oxidative stress and ER stress, with specific emphasis on heme oxygenase-1 (HO-1). In human ECs, we determined the effects of Hcy and Pic on annexin V positivity, glucose-regulated protein 78 kDa (GRP78) and C/EBP homologous protein (CHOP) expression, X-box binding protein 1 (Xbp-1) mRNA slicing, and ROS-sensitive dihydroethidium (DHE) oxidation. Hcy increased annexin V-positive cells, DHE oxidation, GRP78 and CHOP expression and Xbp-1 mRNA splicing, indicating that Hcy induces apoptosis, oxidative stress and ER stress. Pretreatment of ECs with Pic significantly inhibited Hcy-induced apoptosis, ROS generation and ER stress. Pic also increased HO-1 expression via activation of nuclear factor-E2-related factor 2 (Nrf2). Interestingly, the inhibitory effects of Pic on Hcy-induced apoptosis, ROS generation and ER stress were abolished by down-regulation of HO-1 expression, while mimicked by treatment of ECs with the HO-1 inducer hemin. Overall, these results suggest that Pic may protect ECs against Hcy-induced apoptosis, oxidative stress and ER stress via Nrf2-dependent HO-1 expression.
journal_name
Amino Acidsjournal_title
Amino acidsauthors
Kil JS,Jeong SO,Chung HT,Pae HOdoi
10.1007/s00726-016-2375-0subject
Has Abstractpub_date
2017-04-01 00:00:00pages
735-745issue
4eissn
0939-4451issn
1438-2199pii
10.1007/s00726-016-2375-0journal_volume
49pub_type
杂志文章相关文献
AMINO ACIDS文献大全abstract::Several descriptors of protein structure at the sequence and residue levels have been recently proposed. They are widely adopted in the analysis and prediction of structural and functional characteristics of proteins. Numerous in silico methods have been developed for sequence-based prediction of these descriptors. Ho...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0721-1
更新日期:2011-03-01 00:00:00
abstract::The leptin receptor antagonist peptide Allo-aca exhibits picomolar activities in various cellular systems and sub-mg/kg subcutaneous efficacies in animal models making it a prime drug candidate and target validation tool. Here we identified the biochemical basis for its remarkable in vivo activity. Allo-aca decomposed...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-013-1650-6
更新日期:2014-04-01 00:00:00
abstract::Animals at the neonatal stage have to eat more to support better growth and health. However, it is difficult to understand the mechanism of feeding during an early stage of life in the brain of the rodent model. Chickens are precocial and they can look for their food by themselves right after hatching. Neonatal chicks...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-019-02762-x
更新日期:2019-08-01 00:00:00
abstract::Dietary polyamines and amino acids (AAs) are crucial for human growth, development, reproduction, and health. However, the scientific literature shows large variations in polyamine and AA concentrations among major staple foods of plant origin, and there is a scarcity of information regarding their complete compositio...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-019-02751-0
更新日期:2019-08-01 00:00:00
abstract::M33 is a branched peptide currently under preclinical characterization for the development of a new antibacterial drug against gram-negative bacteria. Here, we report its pegylation at the C-terminus of the three-lysine-branching core and the resulting increase in stability to Pseudomonas aeruginosa elastase. This pro...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-014-1686-2
更新日期:2014-05-01 00:00:00
abstract::In mammals, two major routes exist for the metabolic conversion of L-glutamine to α-ketoglutarate. The most widely studied pathway involves the hydrolysis of L-glutamine to L-glutamate catalyzed by glutaminases, followed by the conversion of L-glutamate to α-ketoglutarate by the action of an L-glutamate-linked aminotr...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-015-2061-7
更新日期:2016-01-01 00:00:00
abstract::Chemically defined amino acid diets have been developed for most laboratory and meat-producing animal species as well as for humans. In many cases, growth performance of animals fed these diets equals that obtained with standard intact-protein diets. The pattern of both essential and nonessential amino acids is critic...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00806073
更新日期:1992-02-01 00:00:00
abstract::The successful prediction of protein-folding rates based on the sequence-predicted secondary structure suggests that the folding rates might be predicted from sequence alone. To pursue this question, we directly predict the folding rates from amino acid sequences, which do not require any information on secondary or t...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1189-3
更新日期:2012-08-01 00:00:00
abstract::Lateral diffusion of membrane-integral receptors within the plane of the membrane has been postulated to be mechanistically important for signal transduction. Direct measurement of polypeptide hormone receptor lateral mobility using fluorescence photobleaching recovery techniques indicates that tyrosine kinase recepto...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00805831
更新日期:1995-06-01 00:00:00
abstract::Seryl-histidine dipeptide (Ser-His) has been recognized as the shortest peptide with hydrolysis cleavage activity; however, its protein cleavage spectrum has not yet been fully explored. Here, four differently folded proteins were treated with Ser-His, and the digestion products were evaluated with high-resolution mas...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-017-2487-1
更新日期:2018-01-01 00:00:00
abstract::This study determined whether N-acetylcysteine (NAC) could affect intestinal redox status, proinflammatory cytokines, epidermal growth factor (EGF), EGF receptor (EGFR), Toll-like receptor-4 (TLR4), and aquaporin-8 in a lipopolysaccharide (LPS)-challenged piglet model. Eighteen piglets (35-day-old) were randomly alloc...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1295-x
更新日期:2013-09-01 00:00:00
abstract::The purpose of this study was to investigate H(e) concentration in plasma from 80 healthy donors in relation to age (6 newborns are also included), sex, daily variation (9, 11 a.m.; 2, 6, 12 p.m.) and a period of 5 subsequent months. A significant correlation (r = 0.63, p < 0.001) was observed between plasma H(e) and ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00806188
更新日期:1993-02-01 00:00:00
abstract::The effects of metabotropic glutamate receptor (mGluR) activation were studied in medium spiny neurons and large aspiny (LA) interneurons by means of electrophysiological and optical recordings. DCG-IV and L-SOP, agonists for group II and III mGluRs, respectively, produced a presynaptic inhibitory effect on corticostr...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s007260070040
更新日期:2000-01-01 00:00:00
abstract::Early evaluation of cancer response to a therapeutic regimen can help increase the effectiveness of treatment schemes and, by enabling early termination of ineffective treatments, minimize toxicity, and reduce expenses. Biomarkers that provide early indication of tumor therapy response are urgently needed. Solid tumor...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0548-9
更新日期:2011-11-01 00:00:00
abstract::Oxidative stress, an excess of reactive oxygen species (ROS), may lead to oxidative post-translational modifications of proteins resulting in the cleavage of the peptide backbone, known as α-amidation, and formation of fragments such as peptide amides and α-ketoacyl peptides (α-KaP). In this study, we first compared d...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-020-02902-8
更新日期:2020-10-01 00:00:00
abstract::The synthesis of enantiomerically pure 2-aminocyclobutanecarboxylic acids has been refined to improve both the efficiency and the simplicity. These improvements provide a shorter and easier access to the racemic cis-cyclobutane β-amino acid core. Derivatization of this material with a chiral non-racemic oxazolidin-2-o...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-0918-y
更新日期:2011-08-01 00:00:00
abstract::The G protein-coupled receptor kinase (GRK) family consists of seven cytosolic serine/threonine (Ser/Thr) protein kinases, and among them, GRK2 is involved in the regulation of an enormous range of both G protein-coupled receptors (GPCRs) and non-GPCR substrates that participate in or regulate many critical cellular p...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-020-02864-x
更新日期:2020-07-01 00:00:00
abstract::On a biochemical level, conventional antidepressants have been shown to modulate synaptic levels of biogenic amines (i.e., serotonin, norepinephrine, and dopamine), most often by interfering with reuptake processes or inhibiting metabolism. Strategies directed at modulating glutamatergic transmission may overcome the ...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-001-0121-7
更新日期:2002-01-01 00:00:00
abstract::Germination ofBacillus subtilis spores was initiated by L-Ala and competitively inhibited by D-Ala, suggesting the presence of an alanine receptor. The spores showed alanine racemase activity in the spore coat. To investigate the role of alanine racemase (L → D) on germination, net racemase activity was determined usi...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00805804
更新日期:1993-02-01 00:00:00
abstract::This study tested the hypothesis that L-arginine (Arg) may stimulate cell proliferation and prevent lipopolysaccharide (LPS)-induced death of intestinal cells. Intestinal porcine epithelial cells (IPEC-1) were cultured for 4 days in Arg-free Dulbecco's modified Eagle's-F12 Ham medium (DMEM-F12) containing 10, 100 or 3...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0334-8
更新日期:2010-04-01 00:00:00
abstract::Hypotaurine, concentrated under reduced pressure in HCl solution, partially (30-40%) degrades into taurine (about 30%), 2-aminoethyl-2-aminoethanethiolsulfonate (about 10%) and ethanolamine. The degradation products were identified using LC/APCI-MS, NMR, amino acid analysis and various chromatographies. The identities...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF01320900
更新日期:1998-01-01 00:00:00
abstract::This study aimed to evaluate the effect of taurine (tau) supplementation to low fishmeal (FM) diets on growth performance, oxidative status, and immune response of European seabass juveniles. Four isoproteic (46% crude protein) and isolipidic (19% crude lipid) diets were formulated to contain either 25 or 12.5% FM and...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-019-02769-4
更新日期:2019-09-01 00:00:00
abstract::Among the large family of cyclodepsipeptides, the simplest members are the cyclodidepsipeptides which have an ester group and an amide group in the same six-membered ring. To point out the pharmacological potential of this class of compounds, the present article reviews structure, isolation, synthesis and biological p...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-014-1666-6
更新日期:2014-04-01 00:00:00
abstract::D-Aspartate (D-Asp) is a free D-amino acid detected in multiple brain regions and putative precursor of endogenous N-methyl-D-aspartate (NMDA) acting as agonist at NMDA receptors. In this study, we investigated whether D-Asp (20 mM) in drinking solution for 1 month affects pain responses and pain-related emotional, an...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-016-2205-4
更新日期:2016-07-01 00:00:00
abstract::No systematic approach to detect expression of differentiation-related elements was published so far. The undifferentiated N1E-115 neuroblastoma cell line was switched into a neuronal phenotype by DMSO treatment and used for proteomic experiments. We used two-dimensional gel electrophoresis followed by unambiguous mas...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-005-0256-z
更新日期:2006-10-01 00:00:00
abstract::Green tea which is widely consumed in China, Japan and India, contains polyphenolic compounds, which account for 30% of the dry weight of the leaves. Most of the polyphenols are flavanols, of which (-)-epigallocatechin-3-gallate (EGCG) is most abundant. Epidemiological studies revealed that the incidences of stomach a...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s726-002-8197-9
更新日期:2002-01-01 00:00:00
abstract::Alterations in adipocyte characteristics are highly implicated in the pathology of obesity. In a recent article, we demonstrated that high-fat diet-induced obesity impairs lysosomal function, thereby suppressing autophagy in mice white adipose tissue. Taurine, an amino acid naturally contained in the normal diet and e...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-018-2550-6
更新日期:2018-05-01 00:00:00
abstract::Glycation, or non-enzymatic glycosylation, is a common protein modification formed by reactions between reducing sugars (i.e. aldoses and ketoses) with protein amino groups. Resulting Amadori and Heyns compounds, respectively, can be oxidatively degraded yielding a structurally heterogeneous group of advanced glycatio...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-015-1940-2
更新日期:2015-05-01 00:00:00
abstract::D: -Amino acid oxidase (DAAO) in mammal kidney regulates the renal reactive oxygen species (ROS) levels directly and plays a leading role in the development of ROS-mediated renal pathologic damages based on its crucial role in the oxidative deamination of D: -amino acids and the consequent generation of H(2)O(2). Quan...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0811-0
更新日期:2012-01-01 00:00:00
abstract::The role of post-translational modification of cell proteins with polyamines, a reaction catalyzed by a tissue tranglutaminase (TG, EC 2.3.2.13), in the induction of cell differentiation, represents an intriguing strategy to control cell proliferation and metastatic ability of different tumor cell lines. In this revie...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-008-0157-z
更新日期:2009-04-01 00:00:00