Abstract:
:Although random mutagenesis and screening and evolutionary engineering have long been the gold standards for strain improvement in industry, the development of more sophisticated recombinant DNA tools has led to the introduction of alternate methods for engineering strain diversity. Here, we summarize several combinatorial cell optimization methods developed in recent years, many of which are more amenable to phenotypic transfer and more efficient in probing greater dimensions of the available phenotypic space. They include tools that enable the fine-tuning of pathway expression (synthetic promoter libraries, tunable intergenic regions (TIGRs)), methods for generating randomized knockout and overexpression libraries, and more global techniques (artificial transcription factor engineering, global transcription machinery engineering, ribosome engineering, and genome shuffling) for eliciting complex, multigenic cellular properties.
journal_name
Curr Opin Chem Bioljournal_title
Current opinion in chemical biologyauthors
Santos CN,Stephanopoulos Gdoi
10.1016/j.cbpa.2008.01.017subject
Has Abstractpub_date
2008-04-01 00:00:00pages
168-76issue
2eissn
1367-5931issn
1879-0402pii
S1367-5931(08)00007-0journal_volume
12pub_type
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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abstract::Although new detection screening methods must still be developed, the actual main limitation in combinatorial chemistry seems to be the diversity of ligands that can be generated in terms of real structural and chemical diversity. Thus, there is a strong interest for the development of different strategies for the par...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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