Determining molecular weight distributions of antigen-antibody complexes by quasi-elastic light scattering.

Abstract:

:Physiological properties of soluble antigen-antibody (Ag-Ab) complexes depend in part on the size of the complexes. In previous work, the size distribution and structure of model Ag-Ab complexes were determined by electron microscopy. In this study, we used constrained regularization analysis of quasi-elastic light scattering data to estimate molecular weight distributions of model Ag-Ab complexes. A conformational model was necessary to determine appropriate correlations between molecular weight and diffusion coefficient, and to estimate particle structure factors. Porod-Kratky theory proved to be an adequate conformational model for these purposes. The molecular weight distributions determined by constrained regularization compared favorably with distributions obtained either by electron microscopy or by thermodynamic modeling.

journal_name

Biopolymers

journal_title

Biopolymers

authors

Murphy RM,Yarmush ML,Colton CK

doi

10.1002/bip.360311107

subject

Has Abstract

pub_date

1991-10-01 00:00:00

pages

1289-95

issue

11

eissn

0006-3525

issn

1097-0282

journal_volume

31

pub_type

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