Abstract:
:Physiological properties of soluble antigen-antibody (Ag-Ab) complexes depend in part on the size of the complexes. In previous work, the size distribution and structure of model Ag-Ab complexes were determined by electron microscopy. In this study, we used constrained regularization analysis of quasi-elastic light scattering data to estimate molecular weight distributions of model Ag-Ab complexes. A conformational model was necessary to determine appropriate correlations between molecular weight and diffusion coefficient, and to estimate particle structure factors. Porod-Kratky theory proved to be an adequate conformational model for these purposes. The molecular weight distributions determined by constrained regularization compared favorably with distributions obtained either by electron microscopy or by thermodynamic modeling.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Murphy RM,Yarmush ML,Colton CKdoi
10.1002/bip.360311107subject
Has Abstractpub_date
1991-10-01 00:00:00pages
1289-95issue
11eissn
0006-3525issn
1097-0282journal_volume
31pub_type
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