Abstract:
:The existence of an "RNA world" as an early step in the history of life increases the interest for the characterization of these biomolecules. The hairpin ribozyme studied here is a self-cleaving/ligating motif found in the minus strand of the satellite RNA associated with Tobacco ringspot virus. Surface-enhanced Raman spectroscopy (SERS) is a powerful tool to study trace amounts of RNA. In controlled conditions, a SERS signal is proportional to the amount of free residues adsorbed on the metal surface. On RNA cleavage, residues are unpaired and free to interact with metal. SERS procedures are used to monitor and quantify the catalysis of ribozyme cleavage at biological concentrations in real time; thus, they propose an interesting alternative to electrophoretic methods.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Percot A,Lecomte S,Vergne J,Maurel MCdoi
10.1002/bip.21143subject
Has Abstractpub_date
2009-05-01 00:00:00pages
384-90issue
5eissn
0006-3525issn
1097-0282journal_volume
91pub_type
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