Abstract:
:Previous studies have demonstrated that His 12 plays a major role in the pH-dependent stability of the helix formed by the isolated C-peptide (residues 1-13 of ribonuclease A). Here, amino acid replacement experiments show that His 12+ stabilizes the C-peptide helix chiefly by interacting with Phe 8. The Phe 8 ... His 12+ ring interaction is specific for the protonated form of His 12 (His 12+) and the interaction is not screened significantly by NaCl, unlike the charged group ... helix dipole interactions studied earlier in C-peptide. Analogs of C-peptide that are unable to form the Phe 8 ... His 12+ interaction show large increases in helix content for Phe----Ala and His----Ala. Therefore, the helical tendencies of the individual residues Phe, His, and Ala are important in determining the result of a replacement experiment. Since the side chains of Phe 8 and His 12 probably interact within the N-terminal helix of ribonuclease A, the existence of the Phe 8 ... His 12+ interaction in the isolated C-peptide helix adds to the evidence that the C-peptide helix is an autonomous folding unit.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Shoemaker KR,Fairman R,Schultz DA,Robertson AD,York EJ,Stewart JM,Baldwin RLdoi
10.1002/bip.360290104subject
Has Abstractpub_date
1990-01-01 00:00:00pages
1-11issue
1eissn
0006-3525issn
1097-0282journal_volume
29pub_type
杂志文章相关文献
BIOPOLYMERS文献大全abstract::A desire to replicate the structural and functional complexity of proteins with structured, sequence-specific oligomers motivates study of the structural features of water-soluble peptoids (N-substituted glycine oligomers). Understanding the molecular-level details of peptoid self-assembly in water is essential to adv...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.23248
更新日期:2019-04-01 00:00:00
abstract::We describe a method to determine the magnitude of protein-induced DNA bends relative to a set of standard A tract bends using comparative gel electrophoresis. The DNA bend of interest was that induced by the catabolite activator protein (CAP), the transcriptional activator protein of the lac operon. The set of compar...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360290106
更新日期:1990-01-01 00:00:00
abstract::Reactive oxygen species (ROS) have generated a great deal of interest in the clinical field since experimental studies showed the involvement of these species in carcinogenesis. This paper reports the detection of ROS during the decomposition of H2O2 in the presence of samples obtained from tissues of 16 patients with...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20068
更新日期:2004-08-05 00:00:00
abstract::A conformational study of poly-L-serine has shown that it can exist in the left-handed α-helical form. A study of a pair of peptide units with the serine sidegroup attached to the α carbon atom linking the two units showed that OH ⃛O hydrogen bonds between the OH group of the side chain and a carbonyl oxygen of the f...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.1968.360060403
更新日期:1968-04-01 00:00:00
abstract::Physiological properties of soluble antigen-antibody (Ag-Ab) complexes depend in part on the size of the complexes. In previous work, the size distribution and structure of model Ag-Ab complexes were determined by electron microscopy. In this study, we used constrained regularization analysis of quasi-elastic light sc...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360311107
更新日期:1991-10-01 00:00:00
abstract::Polymeric macromolecules, when viewed abstractly as strings of symbols, can be treated in terms of formal language theory, providing a mathematical foundation for characterizing such strings both as collections and in terms of their individual structures. In addition this approach offers a framework for analysis of ma...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.22101
更新日期:2013-03-01 00:00:00
abstract::The present study characterizes the unfolding and folding processes of recombinant manganese peroxidase. This enzyme contains five disulfide bonds, two calcium ions, and one heme prosthetic group. Circular dichroism in the far UV was used to monitor global changes of the protein secondary structure, whereas UV-visible...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10283
更新日期:2003-01-01 00:00:00
abstract::Recently, we isolated and purified a neutral polysaccharide (PGN) from edible fungus Pleurotus geestanus. Its structure was characterized by a range of physical-chemical methods, including high performance anion exchange chromatography, uronic acid, and protein analyses, size exclusion chromatography with ultraviolet,...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21303
更新日期:2010-02-01 00:00:00
abstract::It has recently been reported that synthetic peptides corresponding to the C-terminal sequence of G alpha, can be used to study the molecular mechanisms of interaction between this protein and G protein coupled receptors (Hamm et al., Science, 1988, Vol. 241, pp. 832-835). A conformational analysis on a 11 amino acids...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(200009)54:3<186::AID-BIP50>3.0.C
更新日期:2000-09-01 00:00:00
abstract::Because of its involvement in HIV entry, the chemokine receptor CXCR4 is an attractive target for antiretroviral drugs. Despite the large number of CXCR4 inhibitors studied, the 3D pharmacophore for binding to CXCR4 remains elusive, mainly as a result of conformational flexibility inherent in the identified ligands. I...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20508
更新日期:2006-01-01 00:00:00
abstract::Constrained azapeptides were designed based on the Ala-Val-Pro-Ile sequence from the second mitochondria-derived activator of caspases (Smac) protein and tested for ability to induce apoptosis in cancer cells. Diels-Alder cyclizations and Alder-ene reactions on azopeptides enabled construction of a set of constrained ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22851
更新日期:2016-05-01 00:00:00
abstract::The self-cleaving hepatitis delta virus (HDV) ribozyme is essential for the replication of HDV, a liver disease causing pathogen in humans. The catalytically critical nucleotide C75 of the ribozyme is buttressed by a trefoil turn pivoting around an extruded G76. In all available crystal structures, the conformation of...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20693
更新日期:2007-04-05 00:00:00
abstract::The formation mechanism of an alanine-based peptide has been studied by all-atom molecular dynamics simulations with a recently developed all-atom point-charge force field and the Generalize Born continuum solvent model at an effective salt concentration of 0.2M. Thirty-two simulations were conducted. Each simulation ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10216
更新日期:2003-01-01 00:00:00
abstract::The aminoacyl-tRNA synthetases are an ancient group of enzymes that catalyze the covalent attachment of an amino acid to its cognate transfer RNA. The question of specificity, that is, how each synthetase selects the correct individual or isoacceptor set of tRNAs for each amino acid, has been referred to as the second...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0282(1999)52:1<1::AID-BIP1>3.0.
更新日期:1999-01-01 00:00:00
abstract::For systems involving highly and oppositely charged proteins, electrostatic forces dominate association and contribute to biomolecular complex stability. Using experimental or theoretical alanine-scanning mutagenesis, it is possible to elucidate the contribution of individual ionizable amino acids to protein associati...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21644
更新日期:2011-11-01 00:00:00
abstract::Surfactant protein C (SP-C) is an important constituent of lung surfactant (LS) and, along with SP-B, is included in exogenous surfactant replacement therapies for treating respiratory distress syndrome (RDS). SP-C's biophysical activity depends upon the presence of a rigid C-terminal helix, of which the secondary str...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.23277
更新日期:2019-06-01 00:00:00
abstract::Short peptides composed of phenylalanine and sequences derived from amyloidogenic peptides have the ability to self-assemble to form nanostructures including hydrogels. The self-assembly of peptides composed of only hydrophobic amino acids and aliphatic protecting groups have not been investigated in detail. We have e...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.23033
更新日期:2017-11-01 00:00:00
abstract::Cyclic pentapeptides are excellent models for reverse turns and have been used extensively in our laboratory to explore the influence of different amino acid sequences on turn preference. This paper is divided into two parts: In the first, we review our previous studies of cyclic pentapeptides. We summarize work that ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360290130
更新日期:1990-01-01 00:00:00
abstract::Shorter analogues of a continuous epitope of hepatitis A virus, VP3(110-121) peptide, failed to react with convalescent sera, indicating the importance of the entire peptide in the epitope structure. To better understand the influence of the structural properties of this 12-mer peptide epitope on its biological activi...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(200012)54:7<477::AID-BIP10>3.0.C
更新日期:2000-12-01 00:00:00
abstract::Peptide-conjugated polysaccharide matrices using bioactive laminin-derived peptides are useful biomaterials for tissue and cell engineering. Here, we demonstrate an easy handling preparation method for peptide-polysaccharide matrices using polyion complex with both alginate and chitosan. First, aldehyde-alginate was s...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22983
更新日期:2017-01-01 00:00:00
abstract::A novel approach to sequentially degrade peptoid N-terminal N-(substituted)glycine residues on the solid-phase using very mild conditions is reported. This method relies on the treatment of resin-bound, bromoacetylated peptoids with silver perchlorate in THF, leading to an intramolecular cyclization reaction to libera...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22884
更新日期:2016-09-01 00:00:00
abstract::Tissue engineering techniques particularly using electrospun scaffolds have been intensively used in recent years for the development of small diameter vascular grafts. However, the development of a completely successful scaffold that fulfills multiple requirements to guarantee complete vascular regeneration remains c...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22524
更新日期:2014-12-01 00:00:00
abstract::The solution conformations of three trispeptides--L,L,L-1,3,5-C6H3[CH2NHCOCH(X)-NHBoc++ +]3, X = CH3 (Ala) or CH2CH(CH3)2 (Leu), and L,L,L-N(CH2CH2NHCOCH[CH2-CH(CH3)2]NHBoc)3--have been determined from their ir and vibrational CD (VCD) spectra in the NH stretching and carbonyl stretching regions in apolar solution. Th...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360320706
更新日期:1992-07-01 00:00:00
abstract::The aim of this study was to develop a nondestructive method to quantitate relative amounts of n-3 and n-6 polyunsaturated fatty acid (PUFA) species in vegetable oils and oil seeds using Fourier transform IR spectroscopy (FTIR). The alkene Cbond;H stretching vibrations of unsaturated fatty acids in oils showed IR abso...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10505
更新日期:2003-12-01 00:00:00
abstract::Chiral interaction of helical peptide with chiral molecule, and concomitant induction in its helix sense have been demonstrated in optically inactive nonapeptide (1) possessing Gly at its N-terminus: H-Gly-(Delta(Z)Phe-Aib)(4)-OCH(3) (1: Delta(Z)Phe = Z-dehydrophenylalanine; Aib = alpha-aminoisobutyric acid). Spectros...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20557
更新日期:2006-11-01 00:00:00
abstract::A series of CD measurements were carried out on members of peptides in the endothelin and sarafotoxin families. The helical structures taken by these peptides containing the helical motif with the sequences of Cys-X-X-X-Cys and Cys-X-Cys [Y. Kobayashi et al. (1991) Neurochemistry International Vol. 18, pp. 525-534] ar...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360320410
更新日期:1992-04-01 00:00:00
abstract::The applicability of the FTIR attenuated total reflectance technique for in situ monitoring of plant physiological processes such as leaf senescence and aging has been examined. Difference spectra obtained by subtracting the spectrum of the young plant leaf from that of the older one revealed positive bands at 1650-15...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10297
更新日期:2003-01-01 00:00:00
abstract::Infrared spectra of the amino acid residues in H2O solution have been obtained in the 1800-1400-cm-1 region. It has been established that amino acid residues of arginine, asparagine, glutamine, aspartic and glutamic acids, lysine, tyrosine, histidine, and phenylalanine have intensive absorption in this spectral region...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360301309
更新日期:1990-01-01 00:00:00
abstract::Bleomycins A(2) and B(2) are the two active components in the antineoplastic drug Blenoxane. DNA is targeted by this drug in cancer cells and the mode of action of this drug involves DNA binding. Ambiguity exists as to the way in which bleomycin binds to DNA. Raman spectroscopy was used to examine both calf thymus DNA...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(1999)52:3<110::AID-BIP20>3.0.CO;
更新日期:1999-01-01 00:00:00
abstract::The title compound represents the smallest member of cyclic proline peptides corresponding to the general formula c(DDLL-Pro4)n with a strictly D,D,L,L double-alternating sequence of the chiral amino acid residues. The cyclopeptides with n greater than or equal to 2 could be synthesized from both DDLL-Pro4 (1) and DLL...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360310618
更新日期:1991-05-01 00:00:00