Abstract：
:An inadequate balance between oxidant species and antioxidant mechanisms may constitute the primary mechanisms of a number of pathologies. The liver plays a central role in this balance: parenchymal hepatic cells contain and export especially high levels of the antioxidant glutathione and activated Kupffer cells release inflammation mediators and reactive oxygen species. There is growing evidence of a paracrine regulation of hepatic function by means of a fluent intercellular communication which must still be fully elucidated, especially in basal conditions. In vivo models provide often too complex results but, in vitro, tissue interactions are left aside; therefore it is important to find new experimental models to address cell communication studies. Here we propose the complementary use of three models to study liver glutathione system regulation in basal conditions: pure parenchymal cells primary cultures, addition of sinusoidal cell conditioned media to parenchymal cells and co-culture of sinusoidal cells using porous membranes. We have also developed a high specifity immunofluorescent method for the complete characterization of sinusoidal cell populations by flow cytometry and confocal microscopy. Our results show that Kupffer cells possess higher levels of reactive oxygen species than sinusoidal endothelial cells even in basal conditions. We also report that the glutathione content of hepatic parenchymal cells in basal conditions is regulated by a sinusoidal-parenchymal cells cross-talk and suggest the existence of a paracrine circuit in the management of liver oxidative stress.
journal_name
Histol Histopatholjournal_title
Histology and histopathologyauthors
Catalá M,Pagani R,Portolés MTdoi
10.14670/HH-22.399subject
Has Abstractpub_date
2007-04-01 00:00:00pages
399-407issue
4eissn
0213-3911issn
1699-5848journal_volume
22pub_type
杂志文章abstract：:The salamanderfish, Lepidogalaxias salamandroides (Galaxiidae, Teleostei) is endemic to southwestern Australia and inhabits shallow, freshwater pools which evaporate during the hot summer months. Burrowing into the substrate in response to falling water levels allows these fish to aestivate for extended periods of tim...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-13.1037
更新日期：1998-10-01 00:00:00
abstract：:High mobility group box 1 protein (HMGB1) is a non-histone chromosomal protein which is highly conserved, ubiquitous, and widely distributed. HMGB1 has multiple functions in the nucleus, including the maintenance of nucleosome structure, the regulation of gene transcription, and involvement in DNA recombination. HMBG1...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-29.235
更新日期：2014-02-01 00:00:00
abstract：:Macrophages are important effector cells in cell-mediated immunity against intracellular infection. Among cytokines that macrophages are able to release are IL-12 and TNF alpha. IL-12 is a critical linker between the innate and adaptive cell-mediated immunity, capable of Th1 differentiation and IFN gamma release by T ...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-15.199
更新日期：2000-01-01 00:00:00
abstract：:In order to analyze the incidence and prevalence of Human Papillomavirus (HPV) in penile carcinoma, we studied 49 patients with penile carcinoma. Formalin-fixed, paraffin-embedded tissue samples were collected from 64 samples of penile carcinoma from the Hospital General Universitario (Albacete, Spain). Cases were his...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-22.177
更新日期：2007-02-01 00:00:00
abstract：:Cyclosporin A is an immunosuppressive drug, which disrupts the activation of peripheral T-lymphocyte pool and blocks the maturation of thymocytes within the thymus. Normally, thymic nonlymphoid cells provide the optimal inductive microenvironment for development of T-lymphocytes. After application of cyclosporin A the...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-13.1183
更新日期：1998-10-01 00:00:00
abstract：:Terminal dendrites of cone horizontal cells (HCs) in teleost retinas show numerous spine-like protrusions named spinules, which are invaginated into the cone pedicles during light-adaptation, but retracted during dark-adaptation. Somata of HC show nematosomes whose size decreases as the number of spinules increases. M...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-17.721
更新日期：2002-01-01 00:00:00
abstract：:HNK-1 positive (HNK-1+) cells in human peripheral blood and lymph nodes were comparatively analysed by means of immunohistochemistry and immunoelectron microscopy. In peripheral blood, the HNK-1+ cells were grouped into large granular lymphocytes (LGLs), small lymphocytes and intermediate forms, all of which had many ...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：:The aim of the study was to estimate the significance of oxidative/nitrosative damage and expression of antioxidant enzymes in renal cell carcinomas (RCC). For this we investigated immunohistochemically six antioxidant enzymes (AOEs) including MnSOD, ECSOD, thioredoxin, thioredoxin reductase, and gammaglutamyl cystein...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-21.157
更新日期：2006-02-01 00:00:00
abstract：:To clarify the role of REG IV, a new member of the regenerating gene (REG) family, in tumorigenesis and progression of colorectal carcinoma (CRC), 320 CRC specimens, 123 corresponding adjacent non-cancerous mucosa (ANCMs), 46 corresponding non-adjacent non-cancerous mucosa (NANCMs) and 86 adenomas were investigated im...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-25.473
更新日期：2010-04-01 00:00:00
abstract：:Hexachlorobenzene (HCB) is a fungicide and a pollutant of increasing concern in industrialized regions of the world. Reproductive failure is one of the effects of HCB upon mammals. Alteration of cell shape in the ovary surface epithelium (OSE) of Cynomolgus monkeys following oral administration of HCB was observed in ...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：:Laminin (LAM) and fibronectin (FI) are regarded as major components of the glomerular extracellular matrix. The aim of this study was to define the distribution of LAM and FI in primary glomerulonephritis (GN) and GN of systemic lupus erythematosus (SLE) and to correlate the type of glomerular disorders with possible ...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1993-07-01 00:00:00
abstract：BACKGROUND AND RESULTS:Embryos from diabetic mice exhibit several forms of neural tube defects, including non-closure of the neural tube. In the present study, embryos collected at embryonic day 11.5 from diabetic pregnancies displayed open neural tube with architectural disruption of the surrounding tissues. The perce...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-26.965
更新日期：2011-08-01 00:00:00
abstract：:An electron microscopic study of the myoepithelial cells in the chicken Harderian gland provides evidence that these cells can be transformed into myofibroblasts. After the application of a Brucella ovis suspension in sterile saline onto the eyeball, every 5 minutes for half an hour, myoepithelial cells gradually deve...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1991-07-01 00:00:00
abstract：:The sarcolemmal domain of rat duodenal smooth muscle cells includes caveolae and associated cytoskeletal or filamentous elements. We have used the quick-freezing, deep-etching method to examine the three dimensional relationships between these components. Replica membranes for separated strips of rat duodenal muscle l...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-15.1059
更新日期：2000-10-01 00:00:00
abstract：:This report presents preliminary observations on the distribution of an epithelial surface antigen in human trophoblastic cells studied with the monoclonal antibody Ber-EP4 by means of an immunoperoxidase technique. Three cases of gestational choriocarcinoma and 20 cases of both villous and extravillous trophoblast, i...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
abstract：:Bone morphogenetic proteins (BMPs) belong to the TGF-Beta superfamily and are vital bone inductive factors. BMPs also play important roles during embryonic development and the postnatal homeostasis of various organs and tissues, by controlling cellular differentiation, proliferation and apoptosis. Prostate cancer is t...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-22.1129
更新日期：2007-10-01 00:00:00
abstract：:We compared characteristic lesions occurring in chickens and domestic ducks naturally infected with H5N1 HPAI virus in April and May 2008. Infected chickens generally exhibited pale-green, watery diarrhoea, depression, neurological signs and cyanosis of wattles and combs, and infected ducks generally exhibited neurolo...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-26.167
更新日期：2011-02-01 00:00:00
abstract：:Lymphatic vessels afferent to the popliteal nodes in young adult rats and guinea pigs were interrupted at the lowest edge of the popliteal fossa, and regressive changes in the nodes occurring 4 to 16 or 18 weeks after surgery were re-examined histologically. In the rat, some popliteal nodes were drained by lymphatic c...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1993-07-01 00:00:00
abstract：BACKGROUND:Lipopolysaccharide (LPS) induced inflammation often leads to lung injury, in which pulmonary recruitment of neutrophils plays a pivotal role. Inflammatory processes are influenced by CD26/DPP4, highly expressed in lungs. Asthma induced CD26/DPP4 deficient (CD26/DPP4⁻) Fischer (F) 344 rats suffering from a tr...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-18-117
更新日期：2019-10-01 00:00:00
abstract：:Equine endometritis eosinophilica (EE) is rarely described and its diagnostic criteria are not defined. The aim of this study was to characterize histological features of EE. A data base (1995-2013) was searched for biopsies with increased eosinophils. This study included all biopsies with this diagnosis and represent...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-11-872
更新日期：2017-11-01 00:00:00
abstract：:Snake-like chromatin (SLC) is a nuclear alteration occurring under various pathological conditions and in different tissues. The aim of this study was the morphological and immunocytochemical characterization of SLC-positive conjunctival epithelial cells from keratoconjunctivitis sicca (KCS) patients. Impression cytol...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-21.355
更新日期：2006-04-01 00:00:00
abstract：:Huntington's disease (HD) is a tremendously debilitating disorder that strikes relatively young individuals and progresses rapidly over the next ten to fifteen years inducing a loss of cognitive and motor skills and eventually death occurs. The primary locus of the disorder is a polyglutamine expansion of the protein ...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-23.237
更新日期：2008-02-01 00:00:00
abstract：:Amyloidosis is a group of diseases characterized by the extracellular deposition of protein that contains non-branching, straight fibrils on electron microscopy (amyloid fibrils) that have a high content of beta-pleated sheet conformation. Various biochemically distinct proteins can undergo transformation into amyloid...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-20.1295
更新日期：2005-10-01 00:00:00
abstract：:Mitochondrial transcription factor A (mtTFA) plays a crucial role in both the transcription and maintenance of mitochondrial DNA. A high expression of mtTFA has been demonstrated in several solid tumors, and is closely associated with cancer cell survival/apoptosis and growth. However, its expression pattern in pancre...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-30.193
更新日期：2015-02-01 00:00:00
abstract：:Pneumadin (PNM) is a biologically active decapeptide, which has previously been found to enhanced rat adrenal growth; the mechanism is indirect and probably involves the stimulation of both arginine-vasopressin (AVP) and ACTH release. The effects of 2- and 6-day PNM administration on the atrophic adrenal cortices of r...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：:The main role of growth arrest and DNA damage-inducible (GADD) genes is to block proliferation at G1 and G2 checkpoints in response to DNA damage. The goal of this study was to examine the expression of GADD genes in primary melanomas with respect to prognosis. GADD34 was found in 73% of the primary melanomas investig...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-17.805
更新日期：2002-01-01 00:00:00
abstract：:The presence of Cajal-Retzius cells in the adult human prefrontal and visual cortices has been demonstrated with calcium binding protein immunocytochemistry and NADPH-diaphorase histochemistry. These cells expressed parvalbumin, calbindin and calretinin calcium binding proteins and displayed NADPH-diaphorase enzyme ac...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-14.487
更新日期：1999-04-01 00:00:00
abstract：:Introduction. Lichen planus (LP) is a chronic cytokine-mediated disease of possible auto-immune etiology. 25% of men have anogenital manifestations. Erosive penile LP causes a scarring phimosis of the foreskin in uncircumcised men. Mast cells as potent immune modulators have been implicated in a number of autoimmune a...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-29.1017
更新日期：2014-08-01 00:00:00
abstract：:Studies in a canine bronchogenic carcinoma model indicate that alveolar type II cells may differentiate from carcinogen-exposed epithelium of larger bronchi and generate adenocarcinomas with bronchioloalveolar and other growth patterns. In this study, we investigated whether type II cells are one of the major prolifer...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1997-04-01 00:00:00
abstract：:We used antisera to pure atrial natriuretic peptide to localise this peptide by immunocytochemistry in rat and human tissue. We showed that both rat and human atrial cardiocytes gave a positive reaction while ventricular cardiocytes were consistently negative. Peripheral islet cells in rat but not in human pancreas al...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1986-04-01 00:00:00
