Abstract:
:Studies in a canine bronchogenic carcinoma model indicate that alveolar type II cells may differentiate from carcinogen-exposed epithelium of larger bronchi and generate adenocarcinomas with bronchioloalveolar and other growth patterns. In this study, we investigated whether type II cells are one of the major proliferating cells (= stem cells) in the genesis of two major subsets of bronchogenic carcinoma in humans. Adenocarcinomas (17 bronchioloalveolar; 3 papillary; and 10 other) and squamous cell carcinomas (n = 27) as well as (pre)neoplastic lesions in adjacent bronchi and bronchioles were examined for the presence of type II cell markers and cellular proliferation markers (PCNA; Ki-67) using light and electron microscopy and immunohistochemistry. Distinctive features of type II cells, which do not depend upon the degree of cell maturity, are the approximately cuboid shape, large and roundish nucleus, cytoplasmic staining for surfactant protein A (SP-A), and presence of multilamellar bodies or their precursory forms. Cells with this phenotype were found in early progressive (i.e., dysplastic, in situ, microinvasive) lesions in conducting airways and in all the carcinomas investigated, although with a much greater abundance among glandular lesions compared to squamous lesions. The most consistent sites of type II cells were the basal and adjacent epithelial layers. Nuclear PCNA (Ki-67) expression usually predominated in the same region. None of the lesions displayed specific Clara cell features. Our findings strongly suggest that the type II cell is a pluripotential stem cell in human lung carcinogenesis. Based on our findings in humans and dogs, we postulate that type II tumor stem cells may originate from one of two sources: (1) normal bronchial epithelium (by an oncofetal mechanism of differentiation); and (2) normal alveolar type II cells.
journal_name
Histol Histopatholjournal_title
Histology and histopathologyauthors
Ten Have-Opbroek AA,Benfield JR,van Krieken JH,Dijkman JHsubject
Has Abstractpub_date
1997-04-01 00:00:00pages
319-36issue
2eissn
0213-3911issn
1699-5848journal_volume
12pub_type
杂志文章abstract::We examined the relationship between the incidence of ultrastructural changes in the alveolar septum and the extravascular lung water content. Pulmonary edema was induced in 18 mongrel dogs by either dextran (n = 12) or alloxan (n = 6) administration. Six other dogs served as controls. Extravascular lung water content...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::E(epithelial)-cadherin is a member of a calcium-dependent family of cell surface glycoproteins involved in cell-cell adhesion and morphogenesis. Catenins are a large family of proteins that connect the cadherins to the cytoskeleton. They are important for cadherin function and for transducing signals involved in speci...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-16.1097
更新日期:2001-10-01 00:00:00
abstract::There is now evidence that estrogens and androgens are exerting their effects in different tissues throughout the body. In order to determine the sites of action of these steroids, studies have been performed to identify at the cellular level the localization of androgen receptor (AR) and the two estrogen receptor (ER...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-15.1261
更新日期:2000-10-01 00:00:00
abstract::Chaperone-mediated autophagy (CMA), a selective form of autophagy, where cellular proteins with KFERQ-like motif are targeted to the lysosome for degradation, is necessary to maintain cellular homeostasis. The role of CMA in neurodegenerative diseases has been extensively studied in the past decades, with defects in t...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-18-202
更新日期:2020-07-01 00:00:00
abstract::Using histopathological analysis of semi-thin sections stained with toluidine blue, we observed profound pathological changes in the islets of Langerhans of hamsters infected with the scrapie agent (strain 139H). These included cytoplasmic vesicles, nuclear swelling, and vacuolization in the islet cells. Two types of ...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Although testicular germ cell tumors (TGCTs) are relatively uncommon, they are particularly important, as they tend to affect children and young men, the most common tumor being in males aged from 20 to 40 years, and the incidence has been increasing in the last years. TGCTs comprise two major histologic groups: semin...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-29.999
更新日期:2014-08-01 00:00:00
abstract::Ewing-like sarcomas (ELS) are a heterogenous group of tumors that frequently affect pediatric and young adult patients. Accurate classification and distinction from the Ewing sarcoma family of tumor (ESFT) is decisive in patient management. ELS share a significant morphologic, immunohistochemical and clinical overlap ...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-11-792
更新日期:2016-11-01 00:00:00
abstract::The novel coronavirus disease 2019 (COVID-19) outbreak began in the city of Wuhan, whereupon it rapidly spread throughout China and subsequently across the world. Rapid transmission of COVID-19 has caused wide-spread panic. Many established medications have been used to treat the disease symptoms; however, no specific...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-18-223
更新日期:2020-10-01 00:00:00
abstract::A technique for staining the nucleolar organizer regions (NORs) in tumor cells applied to smears from brain tumor biopsy specimens is described. This technique provides a rapid intraoperative evaluation of the proliferative activity in cerebral neoplasms and is a valuable complement to hematoxylineosin stained smears,...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
abstract::The presence of the mu-opioid receptor and the type of glycosylation in the third extra-cellular loop of this receptor was investigated in the isthmus of mare oviduct during oestrus by means of immunoblotting and immunohistochemistry combined with enzymatic (N-glycosidase F and O-glycosidase) and chemical (beta-elimin...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-23.555
更新日期:2008-05-01 00:00:00
abstract::Neurotrophins are substances that have been shown to be important in growth and remodelling phases in different types of tissue. There is no information concerning the possible occurrences of neurotrophins and their receptors in tendons. In this study, sections of both chronic painful (tendinosis) and pain-free (non-t...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-24.839
更新日期:2009-07-01 00:00:00
abstract::To date, no detailed knowledge from animal experiments is available on the kind and extent of osseous and mineral metabolic disorders in genetically determined, insulin-dependent Type I diabetes. The purpose of this study was to examine the influence of the diabetic metabolic state in spontaneously diabetic BB/O(ttawa...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-18.111
更新日期:2003-01-01 00:00:00
abstract::The immunohistochemical distribution of 15 blood coagulation factors in the germinal centers (GCs) of human Peyer's patches (PPs) was studied. Although factor VIII, active alpha-thrombin, and fibrinogen were hardly evident in the GCs, the majority of coagulation factors, such as kallikrein, high-molecular-weight kinin...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::CD24 is known to be an important diagnostic and prognostic marker of several major cancers affecting females. We aimed to determine CD24 expression in normal, hyperplastic, and carcinomatous endometrium and its correlation with estrogen and progesterone receptor expression. A total of 271 cases including 62 normal/atr...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-24.309
更新日期:2009-03-01 00:00:00
abstract::Ultrastructural changes of the parathyroid glands of pinealectomized golden hamsters were investigated. The main changes in the parathyroid glands 1 hour and 1 day after pinealectomy compared with the control and sham-operated groups were an increase of the Golgi complexes, cisternae of the granular endoplasmic reticu...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
abstract::Cocaine- and amphetamine-regulated transcript peptide (CART) is a neuromediator and/or neuromodulator in nerve structures within the gastrointestinal tract, but knowledge about its distribution, functions and co-localisation with other neuronal factors, especially in humans, is very scarce. During the present investig...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-28.851
更新日期:2013-07-01 00:00:00
abstract::Platelet-derived growth factor-A (PDGF-A) is a locally produced growth factor in the rat testis secreted by both Sertoli cells and Leydig cells. It has been suggested that PDGF-A may be involved in modulation of testosterone production and may be essential to Leydig cell differentiation, however it is not known at wha...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-21.1295
更新日期:2006-12-01 00:00:00
abstract::A quantitative study regarding the age-related changes occurring in the somatic organelles of the neocerebellar Purkinje cell is carried out, using female rats aged 2 to 24 months. Standard manual morphometric techniques are used to calculate the following parameters: somatic volume, volumetric fractions and absolute ...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::Diabetic nephropathy (DN) is a major cause of end-stage renal disease (ESRD), however, specific treatment for DN has not yet been elucidated. Therefore, it is critically important to understand the molecular mechanism underlying DN to develop cause-related therapeutic strategy. To date, various factors such as hemodyn...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-11-777
更新日期:2016-10-01 00:00:00
abstract::We review the morphofunctional characteristics of CD34+ stromal fibroblastic/fibrocytic cells (CD34+ SFCs) and report our observations. We consider the following aspects of CD34+ SFCs: A) The confusing terms applied to this cell type, often combining the prefix CD34 with numerous names, including fibroblasts, fibrocyt...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-29.831
更新日期:2014-07-01 00:00:00
abstract::The population of the world grows every year, and life expectancy tends to increase. Thus, long-term preservation of teeth in aged individuals is an urgent issue. The main causes of tooth loss are well known to be periodontitis, caries, fractures, and orthodontic conditions. Although implant placement is a widely acce...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-29.1249
更新日期:2014-10-01 00:00:00
abstract::Sialoderivatives expressed in the rabbit oviduct under hormonal treatment have been investigated in situ by lectin histochemistry with a view to specifying further regional and temporal specializations which enable ampulla and isthmus to play distinct roles in the reproductive events. Application of MAL II and SNA lec...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-19.1175
更新日期:2004-10-01 00:00:00
abstract::Cell secretion is an important physiological process that ensures smooth metabolic activities, tissue repair and growth and immunological functions in the body. It occurs when the intracellular secretory materials are released to the exterior; these may be in the form of lipids, protein or mucous and may travel throug...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-18.597
更新日期:2003-04-01 00:00:00
abstract::The effects of diethylstilbestrol (DES) and of long-acting somatostatin analog, octreotide (SMS) on the rat anterior pituitary microvasculature have been studied by means of computer-assisted image analysis. Additionally, the effects of DES and SMS on prolactin secretion and anterior pituitary cell proliferation have ...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1997-10-01 00:00:00
abstract::Fluoxetine shows controversial lung effects as it prevents pulmonary hypertension in adult rats but exposure during gestation causes pulmonary hypertension in neonatal rats. In the present study, we tested the null hypothesis that the antidepressant drug fluoxetine does not modify the development of bronchopulmonary d...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-27.1599
更新日期:2012-12-01 00:00:00
abstract::Parkinson's disease (PD) is a neurodegenerative disorder characterized by progressive cell loss confined mostly to dopaminergic neurons of the substantia nigra. Several factors, including oxidative stress, and decreased activity of complex I mitochondrial respiratory chain, are involved in the degenerative process. Ye...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::Sphingosine kinase functions to phosphorylate sphingosine to sphingosine 1-phosphate (S1P) to keep balance in the metabolites of sphingolipids. There are two isoforms of sphingosine kinase, sphingosine kinase 1 (SphK1) and sphingosine kinase 2 (SphK2). Although SphK1 and SphK2 share high sequence similarity, SphK2 has...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-11-939
更新日期:2018-05-01 00:00:00
abstract:BACKGROUND:Lipopolysaccharide (LPS) induced inflammation often leads to lung injury, in which pulmonary recruitment of neutrophils plays a pivotal role. Inflammatory processes are influenced by CD26/DPP4, highly expressed in lungs. Asthma induced CD26/DPP4 deficient (CD26/DPP4⁻) Fischer (F) 344 rats suffering from a tr...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-18-117
更新日期:2019-10-01 00:00:00
abstract::The present study describes the use of a microwave processing protocol for the rapid histopathological and immunohistochemical diagnosis of bovine rabies. Immunohistochemistry has been used for rabies diagnosis in formalin-fixed tissue with satisfactory results, although the time to diagnosis is considerably longer th...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-27.1227
更新日期:2012-09-01 00:00:00
abstract::Various microscopic methods have been used to analyze the morphology and molecular distribution of cells and tissues. Using conventional procedures, however, ischemic or anoxic artifacts are inevitably caused by tissue-resection or perfusion-fixation. The in vivo cryotechnique (IVCT) was developed to overcome these pr...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-22.1281
更新日期:2007-11-01 00:00:00