Galanin and vasoactive intestinal polypeptide: coexistence and corelease from the vascularly perfused pig ileum during distension and chemical stimulation of the mucosa.

Abstract:

:By immunohistochemistry and double staining technique, almost complete coexistence of galanin-like immunoreactivity (GAL-LI) and vasoactive intestinal polypeptide-like immunoreactivity (VIP-LI) was demonstrated in submucosal ganglionic cells and mucosal nerve fibers of the porcine ileum. The release of the two neuropeptides was studied in isolated, vascularly perfused pig ileum. Distension (increasing intraluminal pressure by 10 mm Hg) and intraluminal instillation of homologous gallbladder bile, amino acids, 0.1 M HCl, hypertonic NaCl (3,400 mosm x kg-1) and hypertonic glucose (1,100 mosm x kg-1) all increased the release of GAL-LI and VIP-LI into the venous effluent in parallel. Being the most potent stimulus, bile increased the output of GAL-LI from 0.74 +/- 0.12 to 2.44 +/- 0.81 pmol/min (mean +/- SE, p = 0.018) and the output of VIP-LI from 3.29 +/- 0.19 to 12.68 +/- 4.01 pmol/min (p less than 0.001), respectively. In conclusion, the coexistence and parallel release of GAL and VIP suggest that GAL/VIP neurons may be involved in intramural secretory and motor reflexes.

journal_name

Digestion

journal_title

Digestion

authors

Harling H,Messell T,Poulsen SS,Rasmussen TN,Holst JJ

doi

10.1159/000200741

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

61-71

issue

2

eissn

0012-2823

issn

1421-9867

journal_volume

50

pub_type

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