Abstract:
PURPOSE:To evaluate the ability of pituitary adenylate cyclase-activating polypeptide (PACAP) to induce growth of neuronal processes in cultured trigeminal ganglion cells, and to accelerate neurite outgrowth and recovery of corneal sensitivity after creation of a corneal flap in a rabbit model of laser-assisted in situ keratomileusis (LASIK) surgery. DESIGN:Animal study. METHODS:The cDNA of rabbit PACAP was sequenced, and the expression of PACAP receptors in the trigeminal ganglia from rabbits was quantified by quantitative real-time polymerase chain reaction. Trigeminal ganglion cells were isolated from rabbits and cultured for 48 hours with or without PACAP27 (bioactive N-terminal peptide from PACAP). Cells were stained with antibody against neurofilaments, and neurite outgrowth was quantified by cell counting. In the rabbit LASIK model, a corneal flap with a planned thickness of 130 microm and 8.5 mm diameter was created with a microkeratome. The rabbits then received eyedrops containing PACAP27 four times a day for eight weeks, and corneal sensitivity was measured. Neurite outgrowth was assessed by staining histologic sections of the flap area for cholinesterase. RESULTS:The deduced amino acid sequence of PACAP in rabbit was identical to that of human. PACAP receptor, PAC1, was highly expressed in trigeminal ganglia from newborn and adult rabbits. PACAP27 at 1 microM induced growth of neuronal processes in cultured primary trigeminal ganglion cells. In the LASIK model, extensions of neuronal processes from amputated nerve trunks in cornea were observed after administration of eyedrops containing 1 or 10 microM PACAP27. The 10 microM PACAP27 treatment also greatly accelerated recovery of corneal sensitivity. CONCLUSIONS:PACAP may be a candidate drug for ameliorating dry eye after LASIK surgery.
journal_name
Am J Ophthalmoljournal_title
American journal of ophthalmologyauthors
Fukiage C,Nakajima T,Takayama Y,Minagawa Y,Shearer TR,Azuma Mdoi
10.1016/j.ajo.2006.10.034subject
Has Abstractpub_date
2007-02-01 00:00:00pages
255-262issue
2eissn
0002-9394issn
1879-1891pii
S0002-9394(06)01203-7journal_volume
143pub_type
杂志文章abstract:PURPOSE:To compare the surgical outcomes of ocular surface squamous neoplasia (OSSN) following wide excisional biopsy with and without primary simple limbal epithelial transplantation (p-SLET). DESIGN:Nonrandomized clinical study with historical controls. METHODS:setting: Single-institutional study. PATIENTS:Eight p...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2016.10.021
更新日期:2017-02-01 00:00:00
abstract:PURPOSE:To investigate the relationship between intraretinal macular hemorrhage and visual acuity outcomes in eyes with central retinal vein occlusion or hemiretinal vein occlusion managed with aflibercept, bevacizumab, or observation. DESIGN:Retrospective analysis of data from 2 randomized clinical trials. METHODS:A...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2020.08.030
更新日期:2020-08-20 00:00:00
abstract::Cryotherapy was an effective modality in the treatment of basal cell carcinoma in the eyelid and periorbital area and cured 93% of the 29 lesions in 25 patients. This method was especially advantageous in patients with blood clotting disorders; those with medical contraindications to, or who refused, classical surgery...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/0002-9394(76)90059-3
更新日期:1976-12-01 00:00:00
abstract:PURPOSE/METHODS:We observed an unusual case of lens-induced glaucoma that occurred 65 years after congenital cataract extraction. We successfully treated the patient by removing the residual lens material with pars plana vitrectomy. RESULTS/CONCLUSIONS:The intraocular specimen exhibited lens material, epithelial cells...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0002-9394(14)72563-2
更新日期:1994-12-15 00:00:00
abstract::Follow-up examinations, ranging from four to more than 20 years, were performed on 100 patients with chronic cyclitis whose ages at onset were from 4 to 58 years. Cataracts were found in 42% of eyes and macular disease secondary to macular edema in 28% of eyes. Band keratopathy, glaucoma, retinal detachment, retinosch...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/0002-9394(76)90434-7
更新日期:1976-08-01 00:00:00
abstract::We compared endothelial preservation of paired corneas by scanning electron microscopy after storage in McCarey-Kaufman medium for two or three days to 2.5% chondroitin sulfate (K-Sol) for seven or ten days. One cornea from each of ten pairs of eyes was placed in McCarey-Kaufman medium and the opposite cornea was plac...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0002-9394(14)74106-6
更新日期:1987-11-15 00:00:00
abstract:PURPOSE:To measure the area of the optic nerve subarachnoid space (ONSASA) in patients with normal tension glaucoma (NTG), primary open-angle glaucoma (POAG), and controls and examine its association with relevant ocular and systemic parameters. DESIGN:Cross-sectional study. METHODS:The study included 40 patients wit...
journal_title:American journal of ophthalmology
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.ajo.2017.11.024
更新日期:2018-02-01 00:00:00
abstract::A 50-year-old man who had been taking betamethasone for 20 days experienced a slight bilateral decrease in visual acuity. There were localized dark-gray atrophic lesions at the posterior pole, accompanied by a diffuse detachment of the sensory retina. The retinal vessels appeared to be normal and only minimal anterior...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/0002-9394(82)90526-8
更新日期:1982-03-01 00:00:00
abstract::We successfully treated six patients with culture-proven acute bacterial endophthalmitis by a combination of emergency pars plana vitrectomy and the instillation of intravitreal antibiotics. All six patients regained satisfactory vision. ...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0002-9394(14)76806-0
更新日期:1978-08-01 00:00:00
abstract:PURPOSE:To evaluate serum levels of triamcinolone acetonide after intravitreal high-dose injection. DESIGN:Prospective, interventional case series study. METHODS:For 20 consecutive patients, venous blood samples were taken before and 13 +/- 19 days (range 4 to 92) after an intravitreal injection of 20 to 25 mg triamc...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2004.01.008
更新日期:2004-06-01 00:00:00
abstract::We analyzed the surgical results for 197 eyes that underwent vitrectomy for diabetic traction detachment of the macula. The retina was successfully reattached in 130 of the 197 cases (66%), although the final level of vision depended on the amount of permanent macular damage from the previous detachment and on the eff...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/0002-9394(83)90330-6
更新日期:1983-01-01 00:00:00
abstract:PURPOSE:To investigate the natural history of Stargardt disease (STGD1) using fixation location and fixation stability. DESIGN:Multicenter, international, prospective cohort study. METHODS:Fixation testing was performed using the Nidek MP-1 microperimeter as part of the prospective, multicenter, natural history study...
journal_title:American journal of ophthalmology
pub_type: 杂志文章,多中心研究
doi:10.1016/j.ajo.2018.06.003
更新日期:2018-09-01 00:00:00
abstract:PURPOSE:To learn whether electrophysiological changes indicating amblyopia occur even in the absence of clinically recognizable amblyopia. DESIGN:Prospective study. METHODS:Four consecutive infants between 7 and 19 months of age with unilateral periocular vascular lesions that intermittently obstructed vision in the ...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2008.11.007
更新日期:2009-04-01 00:00:00
abstract:PURPOSE:To compare silicone-hydrogel, poly(2-hydroxyethyl methacrylate) (pHEMA), and phosphorylcholine-coated (PC-C) contact lenses in terms of their susceptibility to biofilm formation by Staphylococcus epidermidis and Pseudomonas aeruginosa. DESIGN:Laboratory investigation. METHODS:Biofilm formation on colonized te...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2008.07.032
更新日期:2009-01-01 00:00:00
abstract::Glycogen, adenosine triphosphate, and hydration were measured in rabbit corneal epithelium to determine whether the corneal epithelium glycogen decrease, increase in epithelial hydration, and decrease in epithelial adenosine triphosphate stores, seen as a result of contact lens wear, were secondary only to anoxia or m...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/0002-9394(75)90884-3
更新日期:1975-07-01 00:00:00
abstract::Vitreous surgery techniques can be used to remove epiretinal tissue associated with various ocular conditions when this tissue covers, distorts, or detaches the macula and causes visual loss. Because the postoperative visual acuity is often between 6/9 (20/30) and 6/30 (20/100), we usually operate on eyes with preoper...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0002-9394(14)74654-9
更新日期:1981-11-01 00:00:00
abstract::Endoscopic intranasal dacryocystorhinostomy has been used as a primary treatment of lacrimal obstruction and for revision of conventional dacryocystorhinostomy. This study correlates dacryocystographic anatomy with the success of the endoscopic surgical technique. Eighteen patients with epiphora and nasolacrimal obstr...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0002-9394(14)71524-7
更新日期:1992-02-15 00:00:00
abstract:PURPOSE:To review the progress and challenges in obtaining accurate corneal power measurements for intraocular lens (IOL) calculations. DESIGN:Personal perspective, review of literature, case presentations, and personal data. METHODS:Through literature review findings, case presentations, and data from the author's c...
journal_title:American journal of ophthalmology
pub_type:
doi:10.1016/j.ajo.2016.08.020
更新日期:2016-11-01 00:00:00
abstract::Study of a father and son who had helicoid peripapillary chorioretinal degeneration suggests that a progressive tearing and retraction of the retinal pigment epithelium or of Bruch's membrane around the optic disk may be involved in the disorder's pathogenesis. We presume that this tearing results primarily from dyspl...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0002-9394(14)74552-0
更新日期:1990-03-15 00:00:00
abstract:PURPOSE:To evaluate whether cost, insurance prior authorization, pregnancy considerations, and subspecialty practice lead to changes in therapeutic decisions among uveitis and retina specialists in the treatment of intermediate and posterior uveitis. DESIGN:Prospective cross-sectional study. METHODS:A total of 934 uv...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2014.10.015
更新日期:2015-02-01 00:00:00
abstract::A 14-year-old boy was stabbed above the left eye and had subsequent loss of both retinal and choroidal perfusion to the posterior segment. Ophthalmoscopic examination ten days after the injury disclosed intense nerve fiber layer edema, most prominent in the posterior pole, and absence of a cherry-red spot. Intravenous...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/0002-9394(79)90666-4
更新日期:1979-10-01 00:00:00
abstract::We reviewed ten patients who had posterior segment complications of acquired syphilis. Five of these patients had a neovascular membrane of the choroid associated with secondary or tertiary syphilis. Vision was stabilized after laser photocoagulation in one patient who had a choroidal neovascular membrane. Five additi...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/0002-9394(89)90433-9
更新日期:1989-11-15 00:00:00
abstract:PURPOSE:To assess the efficacy and safety of systemic propranolol for infantile capillary hemangiomas of the eyelid. DESIGN:Prospective, interventional cases series. METHODS:All patients with eyelid infantile capillary hemangiomas at risk of developing amblyopia seen between January 2009 and January 2012 at the Unive...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2012.06.021
更新日期:2013-01-01 00:00:00
abstract:PURPOSE:To evaluate the microbiology of pediatric orbital cellulitis associated with sinusitis. DESIGN:Retrospective review of medical records of pediatric patients treated for orbital cellulitis. METHODS:All pediatric patients treated for orbital cellulitis associated with sinusitis at Texas Children's Hospital betw...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2007.04.049
更新日期:2007-10-01 00:00:00
abstract::A 33-year-old man had a sudden onset of night blindness 3 1/2 years after intestinal bypass surgery for morbid obesity. Ocular examination disclosed a probable Bitot's spot and electrophysiologic testing showed markedly impaired dark adaptation and subnormal electroretinography. After therapy with orally administered ...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/0002-9394(80)90164-6
更新日期:1980-06-01 00:00:00
abstract:PURPOSE:To determine the frequency of retinal thickening (RT) in eyes with iridocyclitis and to examine the correlations among anterior chamber (AC) inflammation, RT, and visual acuity. DESIGN:Retrospective, observational case series. METHODS:Records were reviewed of patients seen at the Duke University Eye Center Uv...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2009.03.034
更新日期:2009-09-01 00:00:00
abstract::The pathogenesis of reversible cupping of the optic disk in congenital glaucoma was examined by two approaches. Human fetal, neonatal, and adult eyes were examined by histochemistry and electron microscopy to delineate the embryologic development of the optic nerve head. While the neural, glial, and vascular elements ...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/0002-9394(77)90680-8
更新日期:1977-09-01 00:00:00
abstract:PURPOSE:To provide current estimates of the prevalence of diagnosed dry eye disease (DED) and associated demographics among US adults aged ≥18 years. DESIGN:Cross-sectional, population-based survey. METHODS:Data were analyzed from 75 000 participants in the 2013 National Health and Wellness Survey to estimate prevale...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/j.ajo.2017.06.033
更新日期:2017-10-01 00:00:00
abstract:PURPOSE:To report a case of progressive necrotizing fasciitis of the face following acute dacryocystitis. DESIGN:Interventional case report. METHODS:A 60-year-old woman presented with left acute dacryocystitis with abscess formation that had ruptured; a small wound remained. Erythematous swelling of the left eyelid a...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0002-9394(03)00089-8
更新日期:2003-07-01 00:00:00
abstract:PURPOSE:An association of glaucoma and sleep apnea syndrome (SAS) has been widely reported. We investigated the largest group of patients with SAS thus far to determine the prevalence of glaucoma among these patients. DESIGN:Cross-sectional study. METHODS:An institutional study. STUDY POPULATION:A total of 228 patie...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0002-9394(03)00709-8
更新日期:2003-12-01 00:00:00