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Phosphorylcholine impairs susceptibility to biofilm formation of hydrogel contact lenses.

Abstract：

PURPOSE:To compare silicone-hydrogel, poly(2-hydroxyethyl methacrylate) (pHEMA), and phosphorylcholine-coated (PC-C) contact lenses in terms of their susceptibility to biofilm formation by Staphylococcus epidermidis and Pseudomonas aeruginosa. DESIGN:Laboratory investigation. METHODS:Biofilm formation on colonized test lenses was evaluated with confocal microscopy and in vitro antibiotic susceptibility assays. The results of the latter assays were compared with those performed on planktonic cultures of the same organism. RESULTS:For both microorganisms, sessile colonies on silicone-hydrogel and pHEMA lenses displayed lower antibiotic susceptibility than their planktonic counterparts. In contrast, the susceptibility of cultures growing on PC-C lenses was comparable with that for planktonic cultures. In particular, minimum inhibitory concentration for Tazocin (piperacillin plus tazobactam; Wyeth Pharmaceuticals, Aprilia, Italy; S. epidermidis) and gentamicin (P. aeruginosa) was identical, either in the presence of PC-C support or in planktonic cultures (Tazocin,

journal_name

Am J Ophthalmol

journal_title

American journal of ophthalmology

authors

Selan L,Palma S,Scoarughi GL,Papa R,Veeh R,Di Clemente D,Artini M

doi

10.1016/j.ajo.2008.07.032

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

134-9

issue

1

eissn

0002-9394

issn

1879-1891

pii

S0002-9394(08)00584-9

journal_volume

147

pub_type

杂志文章

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