GABAergic tonic inhibition of substantia gelatinosa neurons in mouse spinal cord.

Abstract:

:We used whole-cell recording to identify, for the first time, GABAergic tonic current in subpopulations of substantia gelatinosa neurons in mouse spinal cord slices. Application of the gamma-aminobutyric acid type A receptor antagonist bicuculline revealed tonic currents in 49 of 73 substantia gelatinosa neurons. These tonic currents were dissected into three groups according to their diverse pharmacological properties. In the first group, furosemide failed to block the tonic current while midazolam and propofol potentiated it. In the second group, furosemide blocked the tonic current but midazolam and propofol failed to enhance the current. In the third group, furosemide blocked and 3alpha, 21-dihydroxy-5alpha-pregnan-20-one enhanced the tonic current. The presence of mRNAs for alpha4, alpha5, delta and epsilon subunits in the substantia gelatinosa (reverse-transcriptase polymerase chain reaction) suggest that gamma-aminobutyric acid type A receptors comprising alpha5betaxgamma2, alphaxbetaxepsilon and alpha4betaxdelta subunits are responsible for tonic currents.

journal_name

Neuroreport

journal_title

Neuroreport

authors

Takahashi A,Mashimo T,Uchida I

doi

10.1097/01.wnr.0000230515.86090.bc

subject

Has Abstract

pub_date

2006-08-21 00:00:00

pages

1331-5

issue

12

eissn

0959-4965

issn

1473-558X

pii

00001756-200608210-00021

journal_volume

17

pub_type

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