Phospholipid methylation in skeletal muscle membranes.


:Phospholipid methylation is thought to modulate such vital cellular processes as calcium transport, receptor function, and membrane microviscosity. As these processes are fundamental to the function of muscle cells and are thought to be altered in disease states, we have characterized several features of phospholipid methylation reactions in skeletal muscle and have defined appropriate assay conditions. In rat leg muscle, methyltransferase activity was assayed radiometrically by measuring the incorporation of methyl groups from S-adenosyl-L-[methyl-3H]methionine into membrane phospholipids, the methylated derivatives of which were separated by thin-layer chromatography. Contrary to previous investigations of whole muscle, phospholipid methyltransferase activity was clearly present in skeletal muscle membranes, being highly localized in sarcoplasmic reticulum and present to a lesser extent in sarcolemma. Both the reaction products and the reaction kinetics were consistent with sequential methylation of phospholipids by two methyltransferase enzymes. S-adenosylhomocysteine and its analogues were potent inhibitors of phospholipid methylation in sarcoplasmic reticulum. The predominant localization of phospholipid methyltransferase activity in sarcoplasmic reticulum suggests that its functional role in skeletal muscle may be in calcium transport.


Muscle Nerve


Muscle & nerve


Kuncl RW,Drachman DB,Kishimoto Y




Has Abstract


1985-06-01 00:00:00












  • Invited review: peripheral neuropathy in Sjogren's syndrome.

    abstract::Our experience and review of the literature reveal that Sjogren's syndrome (SS) is an important, poorly recognized cause of peripheral neuropathy. Several forms of peripheral nerve dysfunction occur in SS including trigeminal sensory neuropathy, mononeuropathy multiplex, distal sensory neuropathy, distal sensorimotor ...

    journal_title:Muscle & nerve

    pub_type: 杂志文章,评审


    authors: Kaplan JG,Rosenberg R,Reinitz E,Buchbinder S,Schaumburg HH

    更新日期:1990-07-01 00:00:00

  • Central fatigue assessed by transcranial magnetic stimulation.

    abstract::Central fatigue is a subjective phenomenon which can be examined using transcranial magnetic stimulation (TMS). To assess central fatigue, we compared TMS and peripheral electrical stimulations in patients with central nervous system (CNS) lesions and controls before and after an exhaustive task. The recovery times of...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Liepert J,Kotterba S,Tegenthoff M,Malin JP

    更新日期:1996-11-01 00:00:00

  • Electrophysiologic investigation of thallium poisoning.

    abstract::Electrophysiologic findings in thallium intoxication are usually untimely, limited in extent, and often uninformative. This report documents serial conduction and electromyographic findings in a case of thallium poisoning, beginning 10 days after symptom onset and ending 24 months later. Initially, the plantar nerves ...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Dumitru D,Kalantri A

    更新日期:1990-05-01 00:00:00

  • A theoretical calculation of the electric field induced by magnetic stimulation of a peripheral nerve.

    abstract::A mathematical model is presented that predicts the electric field induced in the arm during magnetic stimulation of a peripheral nerve. The arm is represented as a homogeneous, cylindrical volume conductor. The electric field arises from two sources: the time-varying magnetic field and the accumulation of charge on t...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Roth BJ,Cohen LG,Hallett M,Friauf W,Basser PJ

    更新日期:1990-08-01 00:00:00

  • Clinicopathological study of an autopsy case with sensory-dominant polyradiculoneuropathy with antiganglioside antibodies.

    abstract::A previously reported patient presenting sensory-dominant neuropathy with antiganglioside antibodies, bound preferentially to polysialogangliosides including GD1b, was autopsied. While axonal degeneration was predominant in the sural nerve, many demyelinated fibers were present in the spinal roots. Dorsal roots had un...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Obi T,Murakami T,Takatsu M,Kusunoki S,Serizawa M,Mizoguchi K,Koike R,Nishimura Y

    更新日期:1999-10-01 00:00:00

  • Neuromuscular ultrasound findings in carpal tunnel syndrome with symptoms mainly in the nondominant hand.

    abstract:BACKGROUND:Carpal tunnel syndrome (CTS) may be associated with structural lesions or anatomical variations at the wrist, especially in patients whose symptoms are more severe in, or limited to, the nondominant hand. The aims of this study were to identify the type and frequency of structural abnormalities and anatomica...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Chompoopong P,Preston DC

    更新日期:2020-12-21 00:00:00

  • In vivo bioluminescent imaging of Schwann cells in a poly(DL-lactide-epsilon-caprolactone) nerve guide.

    abstract::Nerve guides seeded with Schwann cells (SCs) promote axonal regeneration in peripheral nerve lesions. We examined the applicability of bioluminescent imaging (BLI) for monitoring the fate of SCs in nerve guides after implantation. Rat SCs were transfected with the firefly luciferase (Fluc) gene and subsequently seeded...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Ma MS,Van Dam G,Meek M,Boddeke E,Copray S

    更新日期:2009-11-01 00:00:00

  • Different contribution of joint and cutaneous inputs to early scalp somatosensory evoked potentials.

    abstract::To elucidate whether the frontal components of scalp somatosensory evoked potentials (SEPs) depend on the type of peripheral input, we compared scalp SEPs in response to electrical stimuli applied to: (i) the proximal phalanx of the thumb, involving both deep and cutaneous afferents; and (ii) the distal phalanx of the...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Restuccia D,Valeriani M,Barba C,Le Pera D,Tonali P,Mauguière F

    更新日期:1999-07-01 00:00:00

  • Mitochondrial capacity, muscle endurance, and low energy in friedreich ataxia.

    abstract:INTRODUCTION:In this study we noninvasively evaluated skeletal muscle mitochondrial capacity, muscle-specific endurance, and energy/fatigue feelings in persons with Friedreich ataxia (FRDA) and able-bodied controls (AB). METHODS:Forearm mitochondrial capacity was measured in FRDA (n = 16) and AB (n = 10) study partici...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Bossie HM,Willingham TB,Schoick RAV,O'Connor PJ,McCully KK

    更新日期:2017-10-01 00:00:00

  • Guidelines for ethical behavior relating to clinical practice issues in neuromuscular and electrodiagnostic medicine.

    abstract::The American Association of Neuromuscular & Electrodiagnostic Medicine (AANEM) developed guidelines to formalize the ethical standards that neuromuscular and electrodiagnostic (EDx) physicians should observe in their clinical and scientific activities. Neuromuscular and EDx medicine is a subspecialty of medicine that ...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Abel NA,De Sousa EA,Govindarajan R,Mayer MP,Simpson DA

    更新日期:2015-12-01 00:00:00

  • Safety of influenza vaccination in patients with myasthenia gravis: a population-based study.

    abstract::Influenza vaccination has been associated with adverse events including Guillain-Barré syndrome. Because the safety of influenza vaccination in patients with myasthenia gravis (MG) has not been established, some clinicians discourage vaccination for these patients. We explored whether the administration of influenza v...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Zinman L,Thoma J,Kwong JC,Kopp A,Stukel TA,Juurlink DN

    更新日期:2009-12-01 00:00:00

  • Slower conduction velocity and motor unit discharge frequency are associated with muscle fatigue during isometric exercise in type 1 diabetes mellitus.

    abstract::Type 1 diabetes mellitus (T1DM) is associated with a peripheral neuropathy that reduces nerve conduction velocity. This may impair high motor-unit discharge frequencies (MUDF), decrease muscle activation, and curtail the ability to sustain repetitive contractile tasks. We examined (1) whether MUDF, the contractile pro...

    journal_title:Muscle & nerve

    pub_type: 临床试验,杂志文章


    authors: Almeida S,Riddell MC,Cafarelli E

    更新日期:2008-02-01 00:00:00

  • Evaluation of patients with recurrent symptoms after ulnar nerve transposition.

    abstract::Focal entrapment of the ulnar nerve occurs most frequently in the region of the elbow, at the ulnar groove or beneath the humeroulnar aponeurosis. Surgical treatment commonly involves transposition of the nerve anterior to the medial epicondyle, in the antecubital fossa. Symptoms may recur after surgery, and, to asses...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Matei CI,Logigian EL,Shefner JM

    更新日期:2004-10-01 00:00:00

  • Twice-weekly glucocorticosteroids in infants and young boys with Duchenne muscular dystrophy.

    abstract:INTRODUCTION:Glucocorticosteroids (GC) are effective in slowing weakness in boys with Duchenne muscular dystrophy (DMD). METHODS:This is a multisite, 1-year, open-label trial of twice-weekly prednisolone (5 mg/kg/dose) in infants/young boys (0.4-2.4 years) with DMD. We compared changes in Bayley III Scales of Infant D...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Connolly AM,Zaidman CM,Golumbek PT,Cradock MM,Flanigan KM,Kuntz NL,Finkel RS,McDonald CM,Iannaccone ST,Anand P,Siener CA,Florence JM,Lowes LP,Alfano LN,Johnson LB,Nicorici A,Nelson LL,Mendell JR,MDA DMD Clinical Resea

    更新日期:2019-06-01 00:00:00

  • Clinical utility of reflex studies in assessing cervical radiculopathy.

    abstract::We prospectively studied the diagnostic utility of upper limb segmental reflexes in patients with suspected cervical radiculopathy (CR). Fifty-three patients (29 men and 24 women), referred for electrodiagnostic testing, were positive for at least one of four clinical criteria for CR: abnormal (1) history, (2) motor (...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Miller TA,Pardo R,Yaworski R

    更新日期:1999-08-01 00:00:00

  • Cross-sectional and longitudinal analysis of an oxidative stress biomarker for spinal and bulbar muscular atrophy.

    abstract:INTRODUCTION:Spinal and bulbar muscular atrophy (SBMA) is an adult-onset motor neuron disease caused by a CAG repeat expansion in the androgen receptor gene. The aim of this study was to verify whether urinary 8-hydroxydeoxyguanosine (8-OHdG), an oxidative stress marker, is a biomarker for SBMA. METHODS:We measured th...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Mano T,Katsuno M,Banno H,Suzuki K,Suga N,Hashizume A,Tanaka F,Sobue G

    更新日期:2012-11-01 00:00:00

  • Muscle does not drive persistent posttraumatic elbow contracture in a rat model.

    abstract:INTRODUCTION:Posttraumatic elbow contracture is clinically challenging because injury often disrupts multiple periarticular soft tissues. Tissue specific contribution to contracture, particularly muscle, remains poorly understood. METHODS:In this study we used a previously developed animal model of elbow contracture. ...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Dunham CL,Chamberlain AM,Meyer GA,Lake SP

    更新日期:2018-12-01 00:00:00

  • Marked increase in CD44-highly positive cells in hyperplastic thymuses from patients with Myasthenia gravis.

    abstract::To investigate the role of the thymus in the pathogenesis of myasthenia gravis (MG), immunohistochemical expression of CD44, CD45R0, B7-1, and IL-2 was studied in: (1) hyperplastic thymuses of patients with MG whose symptoms markedly improved after thymectomy, (2) remnant thymuses of patients with MG whose symptoms di...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Utsugisawa K,Nagane Y,Tohgi H

    更新日期:2000-04-01 00:00:00

  • Conduction and excitability properties of peripheral nerves in end-stage liver disease.

    abstract::The pathophysiology of hepatic neuropathy is poorly understood, but membrane depolarization due to a toxic inhibition of oxidative metabolism has been proposed. We investigated the relationship between nerve excitability properties, nerve dysfunction, and liver function in 11 pretransplant patients, the majority of wh...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Ng K,Lin CS,Murray NM,Burroughs AK,Bostock H

    更新日期:2007-06-01 00:00:00

  • Altered MEF2 isoforms in myotonic dystrophy and other neuromuscular disorders.

    abstract::Because of their central role in muscle development and maintenance, MEF2 family members represent excellent candidate effectors of the muscle pathology in myotonic dystrophy (DM). We investigated the expression and alternative splicing of all four MEF2 genes in muscle from neuromuscular disorder (NMD) patients, inclu...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Bachinski LL,Sirito M,Böhme M,Baggerly KA,Udd B,Krahe R

    更新日期:2010-12-01 00:00:00

  • Estimation of the number and size of human flexor digiti minimi muscle motor units using histological methods.

    abstract::Motor unit (MU) number and size estimates were obtained from the human flexor digiti minimi muscle using histological methods. Ten adult fresh cadavers (33-74 years old) were used. The number of MUs was 130 +/- 15 and the MU size was 108 +/- 10. These values are similar to those reported for other hypothenar muscles, ...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Santo Neto H,de Carvalho VC,Marques MJ

    更新日期:1998-01-01 00:00:00

  • High-repetition cyclic loading is a risk factor for a lumbar disorder.

    abstract::Epidemiological data suggest that prolonged exposure to cyclic lumbar flexion elicits a chronic neuromuscular disorder and disability in workers. This study provides a physiological and biomechanical assessment of various repetitions of cyclic lumbar flexion sessions as a risk factor for development of an acute neurom...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Navar D,Zhou BH,Lu Y,Solomonow M

    更新日期:2006-11-01 00:00:00

  • Further observations on forearm flexor weakness in inclusion body myositis.

    abstract::In order to further characterize and provide a possible mechanism for the asymmetrical involvement of forearm muscles in inclusion body myositis (IBM), we measured isometric hand and pinch grip strength, and forearm muscle girth on 15 IBM patients. Forearm muscle strength and girth were significantly greater on the do...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Felice KJ,Relva GM,Conway SR

    更新日期:1998-05-01 00:00:00

  • Limits of normal nerve function during high-frequency stimulation.

    abstract::We studied the effects of prolonged high-frequency stimulation in normal rat tail nerves. Nerves could conduct impulses at 50 Hz for 5 hours without complete conduction block. However, over time there was a significant decrease in amplitude and increase in latency of the compound nerve action potential (CNAP), which g...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Robinson LR,Nielsen VK

    更新日期:1990-04-01 00:00:00

  • Beta-chemokine receptor expression in idiopathic inflammatory myopathies.

    abstract::Beta-chemokines attract and activate T cells and monocytes and have a key role in chronic inflammation. Certain beta-chemokines, such as monocyte chemoattractant protein-1 (MCP-1), have been reported to be upregulated in the idiopathic inflammatory myopathies (IIM). We studied the distribution of beta-chemokine recept...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: De Paepe B,De Bleecker JL

    更新日期:2005-05-01 00:00:00

  • Creatine kinase activity in normal and Duchenne muscular dystrophy fibroblasts.

    abstract::Cultured human skin fibroblasts from 9 patients with Duchenne muscular dystrophy (DMD) and 8 normal age- and sex-matched controls were examined for creatine kinase (CK) activity. Both the normal and the DMD fibroblasts were found to have significant levels of CK activity (approximately 10 x 10(-3) IU per milligram of ...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Davis MH,Cappel R,Vester JW,Samaha FJ,Gruenstein E

    更新日期:1982-01-01 00:00:00

  • Paradoxical effect of isoproterenol on hamster hereditary polymyopathy.

    abstract::Isoproterenol (ISO), a potent beta-adrenoreceptor agonist, was found to interfere with the development and progression of hamster hereditary polymyopathy. Cytoprotection involved both skeletal and heart muscles with reduced myofibrillar degeneration, phagocytosis, and an unusual scarring process rarely seen at this st...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Jasmin G,Proschek L

    更新日期:1983-07-01 00:00:00

  • Direct dystrophin and reporter gene transfer into dog muscle in vivo.

    abstract::Bacterial beta-galactosidase cDNA was injected without lipofectin into 41 sites in dog muscle and expression was seen in 22 of them. The cDNA and lipofectin was injected into 35 similar sites and expression was seen in 21. Expression was seen in a maximum of 2.5% of muscle fibers and 23.21% of nonmuscle cells. A total...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Howell JM,Fletcher S,O'Hara A,Johnsen RD,Lloyd F,Kakulas BA

    更新日期:1998-02-01 00:00:00

  • Variable presentation of nemaline myopathy: novel mutation of alpha actin gene.

    abstract::Nemaline myopathy is a rare disorder of varying severity and genetic etiology. We present two cases, a father and son, with a novel missense mutation in the alpha actin gene. Both have a history of early motor impairment, with the son's course being considerably more severe. This pair illustrates the clinical variabil...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Bouldin AA,Parisi MA,Laing N,Patterson K,Gospe SM Jr

    更新日期:2007-02-01 00:00:00

  • Acute quadriplegic myopathy: analysis of myosin isoforms and evidence for calpain-mediated proteolysis.

    abstract::Immunocytochemical analysis of muscle specimens from 5 patients with acute quadriplegic myopathy indictes that depletion of either fast or slow myosin occurs in this disorder. The initial lesion consists of focal myosin loss in nonatrophic fibers. Other structural proteins (actin, titin, nebulin) are spared or affecte...

    journal_title:Muscle & nerve

    pub_type: 杂志文章


    authors: Showalter CJ,Engel AG

    更新日期:1997-03-01 00:00:00