Lysenin forms a voltage-dependent channel in artificial lipid bilayer membranes.

Abstract:

:Lysenin, a hemolytic protein derived from the body fluid of earthworm, was incorporated into artificial bilayer membranes. Upon insertion, it formed a voltage-dependent large conductance channel in asolectin bilayers in a sphingomyelin-dependent manner. The channel had low ion-selectivity. Single-channel conductance was calculated as approximately 550 pS in 100 mM KCl. The channel in asolectin bilayers closed when the membrane was held at a positive potential. In contrast, the channel showed no voltage dependency in membranes made of pure phosphatidylcholine and sphingomyelin, suggesting some lipid contents included in the asolectin membranes affected channel gating.

authors

Ide T,Aoki T,Takeuchi Y,Yanagida T

doi

10.1016/j.bbrc.2006.05.115

subject

Has Abstract

pub_date

2006-07-21 00:00:00

pages

288-92

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(06)01183-1

journal_volume

346

pub_type

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