Abstract:
:Lysenin, a hemolytic protein derived from the body fluid of earthworm, was incorporated into artificial bilayer membranes. Upon insertion, it formed a voltage-dependent large conductance channel in asolectin bilayers in a sphingomyelin-dependent manner. The channel had low ion-selectivity. Single-channel conductance was calculated as approximately 550 pS in 100 mM KCl. The channel in asolectin bilayers closed when the membrane was held at a positive potential. In contrast, the channel showed no voltage dependency in membranes made of pure phosphatidylcholine and sphingomyelin, suggesting some lipid contents included in the asolectin membranes affected channel gating.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Ide T,Aoki T,Takeuchi Y,Yanagida Tdoi
10.1016/j.bbrc.2006.05.115subject
Has Abstractpub_date
2006-07-21 00:00:00pages
288-92issue
1eissn
0006-291Xissn
1090-2104pii
S0006-291X(06)01183-1journal_volume
346pub_type
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