Lysenin forms a voltage-dependent channel in artificial lipid bilayer membranes.


:Lysenin, a hemolytic protein derived from the body fluid of earthworm, was incorporated into artificial bilayer membranes. Upon insertion, it formed a voltage-dependent large conductance channel in asolectin bilayers in a sphingomyelin-dependent manner. The channel had low ion-selectivity. Single-channel conductance was calculated as approximately 550 pS in 100 mM KCl. The channel in asolectin bilayers closed when the membrane was held at a positive potential. In contrast, the channel showed no voltage dependency in membranes made of pure phosphatidylcholine and sphingomyelin, suggesting some lipid contents included in the asolectin membranes affected channel gating.


Ide T,Aoki T,Takeuchi Y,Yanagida T




Has Abstract


2006-07-21 00:00:00














  • Proteomic investigations of human HERC2 mutants: Insights into the pathobiology of a neurodevelopmental disorder.

    abstract::HERC2 is a giant protein with E3 ubiquitin ligase activity and other known and suspected functions. Mutations of HERC2 are implicated in the pathogenesis of various cancers and result in severe neurological conditions in Herc2-mutant mice. Recently, a pleotropic autosomal recessive HERC2-associated syndrome of intelle...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Abraham JR,Barnard J,Wang H,Noritz GH,Yeganeh M,Buhas D,Natowicz MR

    更新日期:2019-04-30 00:00:00

  • Expression of Nischarin negatively correlates with estrogen receptor and alters apoptosis, migration and invasion in human breast cancer.

    abstract::Nischarin, a novel integrin binding protein, has been demonstrated its negative effects on cell migration and invasion. However, the biological role of Nischarin in breast cancer has not been fully elucidated yet. Our study aimed to analyze the association between Nischarin expression and clinical features of breast c...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chang C,Wei W,Han D,Meng J,Zhu F,Xiao Y,Wu G,Shi X,Zhang L

    更新日期:2017-03-11 00:00:00

  • Effect of HIF1α on the TRPC6 channel of glomerular podocytes under chronic hypoxia.

    abstract:BACKGROUND:Chronic hypoxia plays an important role in the initiation and progression of chronic renal disease. The pathogenic role of chronic hypoxia in tubulointerstitial injury has been investigated widely, but little is known about acute hypoxia implications in glomerular damage. In this study, we investigated the e...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hu G,He Y,Li Y,Hu X,Liu S,Liao C,Zhang R,Zhou X,Sun H

    更新日期:2021-01-12 00:00:00

  • Structure of an unconventional SH3 domain from the postsynaptic density protein Shank3 at ultrahigh resolution.

    abstract::The Shank family comprises three large multi-domain proteins playing central roles as protein scaffolds in the neuronal postsynaptic density. The Shank proteins are closely linked to neuropsychiatric diseases, such as autism spectrum disorders. One characteristic domain in the Shank family is the SH3 domain, assumed t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ponna SK,Myllykoski M,Boeckers TM,Kursula P

    更新日期:2017-08-26 00:00:00

  • Hydrogen sulfide is produced by cystathionine γ-lyase at the steady-state low intracellular Ca(2+) concentrations.

    abstract::Hydrogen sulfide (H(2)S) is recognized as a physiologic mediator produced in a variety of tissues. It is produced by three enzymes, cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (3MST). However, the regulation of H(2)S production by CSE has not well been understoo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Mikami Y,Shibuya N,Ogasawara Y,Kimura H

    更新日期:2013-02-08 00:00:00

  • Effect of retinoic acid on the acylation of phospholipids in granulocytes in vitro.

    abstract::The influence of retinoic acid on the incorporation of [1-14C]palmitic acid and [1-14C]arachidonic acid into phospholipids was examined in guinea pig peritoneal granulocytes. All-trans-retinoic acid inhibited the incorporation of both fatty acids into phosphatidic acid and phosphatidylinositol. However, it stimulated ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tou J

    更新日期:1983-07-18 00:00:00

  • p21-activated kinase PAK phosphorylates desmin at sites different from those for Rho-associated kinase.

    abstract::p21-activated kinase (PAK) and Rho-associated kinase (Rho-kinase) have been shown to induce Ca(2+)-independent contraction of smooth muscle. PAK-induced contraction of Triton-skinned smooth muscle correlates with increased phosphorylation of caldesmon and desmin, although the role of desmin phosphorylation has remaine...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ohtakara K,Inada H,Goto H,Taki W,Manser E,Lim L,Izawa I,Inagaki M

    更新日期:2000-06-16 00:00:00

  • A coarse-grained Langevin molecular dynamics approach to de novo protein structure prediction.

    abstract::De novo prediction of protein structures, the prediction of structures from amino acid sequences which are not similar to those of hitherto resolved structures, has been one of the major challenges in molecular biophysics. In this paper, we develop a new method of de novo prediction, which combines the fragment assemb...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sasaki TN,Cetin H,Sasai M

    更新日期:2008-05-02 00:00:00

  • Effects of tributyltin on barrier functions in human intestinal Caco-2 cells.

    abstract::The effect of tributyltin (TBT) on human intestinal epithelial cell functions was investigated by using human intestinal Caco-2 cell monolayers. We paid particular attention to the effect of TBT on two barrier functions: the tight junction as a physical barrier and MDR1/P-glycoprotein as a biological barrier. A loss o...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tsukazaki M,Satsu H,Mori A,Sugita-Konishi Y,Shimizu M

    更新日期:2004-03-19 00:00:00

  • Characteristics of carcinoembryonic antigen (CEA) expressed in different cell types: evidence that CEA can function as an adhesion molecule and as a repulsion molecule.

    abstract::The characteristics of carcinoembryonic antigen (CEA) molecules expressed by different cell types have been examined using cDNA transfection into cells of epithelial, neuroectodermal and hematopoietic origin. Variability in the CEA molecules was observed in terms of monoclonal antibody binding, apparent molecular weig...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Grimm T,Riethmüller G,Johnson JP

    更新日期:1994-11-15 00:00:00

  • The 47kDa Akt substrate associates with phosphodiesterase 3B and regulates its level in adipocytes.

    abstract::We have previously described a novel putative 47kDa substrate for the protein kinase Akt (designated AS47) in 3T3-L1 adipocytes. In the present study, we have found by co-immunoprecipitation that AS47 was associated with cyclic nucleotide phosphodiesterase 3B (PDE3B) in lysates of 3T3-L1 adipocytes. The patterns of ex...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chavez JA,Gridley S,Sano H,Lane WS,Lienhard GE

    更新日期:2006-04-21 00:00:00

  • Transcription factor levels in medullary thyroid carcinoma cells differentiated by Harvey ras oncogene: c-jun is increased.

    abstract::In the TT cell line of human medullary thyroid carcinoma, the viral Harvey ras (v-rasH) oncogene induces differentiation, marked by morphological changes, diminution of growth, and increased expression of the calcitonin gene. Here, we show that the transcriptional factor c-jun is increased during v-rasH induced differ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Nelkin BD,Borges M,Mabry M,Baylin SB

    更新日期:1990-07-16 00:00:00

  • Human VAPA and the yeast VAP Scs2p with an altered proline distribution can phenocopy amyotrophic lateral sclerosis-associated VAPB(P56S).

    abstract::A human isoform of the vesicle-associated membrane protein-associated proteins (VAPs), VAPB, causes amyotrophic lateral sclerosis eight due to the missense mutation of Pro-56, whereas human VAPA and the yeast VAP Scs2p proteins are not significantly affected by similar mutations. We have found that VAPA and Scs2p have...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Nakamichi S,Yamanaka K,Suzuki M,Watanabe T,Kagiwada S

    更新日期:2011-01-14 00:00:00

  • The mechanism of action of nitric oxide-donating aspirin.

    abstract::NO-donating aspirin (NO-ASA) is a promising anticancer drug. We studied the contribution of NO-ASA's components (ASA, NO-releasing moiety, and spacer linking them) to its effect. The ASA and NO-releasing moieties play no biological role: ASA inhibits the growth of colon cancer cells >100-fold less potently that NO-ASA...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kashfi K,Rigas B

    更新日期:2007-07-13 00:00:00

  • Identification of a novel murine organic anion transporter family member, OAT6, expressed in olfactory mucosa.

    abstract::The organic anion and cation transporters (OATs and OCTs) are a large family (SLC22) of transmembrane proteins that are able to transport a variety of compounds including drugs, environmental toxins, and endogenous metabolites. OATs are expressed in various tissues, primarily kidney and liver, but also in placenta, sm...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Monte JC,Nagle MA,Eraly SA,Nigam SK

    更新日期:2004-10-15 00:00:00

  • Upstream stimulatory factors regulate the C/EBP alpha gene during differentiation of 3T3-L1 preadipocytes.

    abstract::During adipocyte differentiation, CCAAT/enhancer-binding protein alpha (C/EBPalpha) functions as a pleiotropic transcriptional activator of numerous adipocyte genes. The promoter of the C/EBPalpha gene has an E-box upstream of C/EBP binding site. Deletion or mutation of the E-box decreases promoter activity, suggestin...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kim JW,Monila H,Pandey A,Lane MD

    更新日期:2007-03-09 00:00:00

  • Analysis of laser scattering pattern as an early measure of apoptosis.

    abstract::Light scattering pattern analysis (LSPA) was applied in the current study for accurate and sensitive detection of subtle changes in cell size, which occur in mouse thymocytes undergoing apoptosis. The decrease in cell diameter as measured by LSPA was found to be an early signal of apoptosis preceding the externalizati...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Shiffer Z,Zurgil N,Shafran Y,Deutsch M

    更新日期:2001-12-21 00:00:00

  • Gastric and thymic assay of acute oral treatment of rats with nitric oxide esters of ibuprofen or indomethacin.

    abstract::Two common non-steroidal anti-inflammatory drugs (NSAIDs) and their nitric oxide (NO) adducts were evaluated for effects on stomach and thymus. Following 4-h duration (acute) oral dosing of fasted male Wistar rats, 1.33 x 10(-4)mol/kg of ibuprofen caused significant visual irritation score and microscopic thinning, al...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Downing JE,Madden JC,Ingram MJ,Rostron C

    更新日期:2005-08-26 00:00:00

  • Computing the binding affinity of a ligand buried deep inside a protein with the hybrid steered molecular dynamics.

    abstract::Computing the ligand-protein binding affinity (or the Gibbs free energy) with chemical accuracy has long been a challenge for which many methods/approaches have been developed and refined with various successful applications. False positives and, even more harmful, false negatives have been and still are a common occu...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Villarreal OD,Yu L,Rodriguez RA,Chen LY

    更新日期:2017-01-29 00:00:00

  • Identification of ADAMTS13 peptide sequences binding to von Willebrand factor.

    abstract::ADAMTS13 cleaves multimeric von Willebrand factor (VWF) to regulate VWF-mediated thrombus formation. To search ADAMTS13 peptide sequences binding to VWF, a lambda-phage library expressing various peptides of ADAMTS13 on the surface was screened using VWF either immobilized or in solution under static condition. By the...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Moriki T,Maruyama IN,Igari A,Ikeda Y,Murata M

    更新日期:2010-01-01 00:00:00

  • Proteomics investigation of endogenous S-nitrosylation in Arabidopsis.

    abstract::S-Nitrosylation emerges as an important protein modification in many processes. However, most data were obtained at the protein level after addition of a NO donor, particularly in plants where information about the cysteines nitrosylated in these proteins is scarce. An adapted work-flow, combining the classical biotin...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Fares A,Rossignol M,Peltier JB

    更新日期:2011-12-16 00:00:00

  • Fasting activates the gene expression of UCP3 independent of genes necessary for lipid transport and oxidation in skeletal muscle.

    abstract::Fasting triggers a complex array of adaptive metabolic and hormonal responses including an augmentation in the capacity for mitochondrial fatty acid (FA) oxidation in skeletal muscle. This study hypothesized that this adaptive response is mediated by increased mRNA of key genes central to the regulation of fat oxidati...

    journal_title:Biochemical and biophysical research communications

    pub_type: 临床试验,杂志文章


    authors: Tunstall RJ,Mehan KA,Hargreaves M,Spriet LL,Cameron-Smith D

    更新日期:2002-06-07 00:00:00

  • Tuberous sclerosis complex 2 loss-of-function mutation regulates reactive oxygen species production through Rac1 activation.

    abstract::The products of the TSC1 (hamartin) and TCS2 (tuberin) tumor suppressor genes negatively regulate cell growth by inhibiting mTOR signaling. Recent research has led to the postulation that tuberin and/or hamartin are involved in tumor migration, presumably through Rho activation. Here we show that LEF-8 cells, which co...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Suzuki T,Das SK,Inoue H,Kazami M,Hino O,Kobayashi T,Yeung RS,Kobayashi K,Tadokoro T,Yamamoto Y

    更新日期:2008-03-28 00:00:00

  • Characterization of the novel human transmembrane protein 9 (TMEM9) that localizes to lysosomes and late endosomes.

    abstract::We have identified and characterized the novel human transmembrane protein 9 (TMEM9). TMEM9 encodes a 183 amino-acid protein that contains an N-terminal signal peptide, a single transmembrane region, three potential N-glycosylation sites, and three conserved cys-rich domains in the N-terminus, but no hitherto known fu...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kveine M,Tenstad E,Døsen G,Funderud S,Rian E

    更新日期:2002-10-04 00:00:00

  • IKK regulates the deubiquitinase CYLD at the postsynaptic density.

    abstract::K63-linked polyubiquitination of proteins regulates their trafficking into specific cellular pathways such as endocytosis and autophagy. CYLD, a deubiquitinase specific for K63-linked polyubiquitins, is present in high quantities at the postsynaptic density (PSD). It was previously shown that, under excitatory conditi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Thein S,Pham A,Bayer KU,Tao-Cheng JH,Dosemeci A

    更新日期:2014-07-18 00:00:00

  • Cyclo (His-Pro), a metabolite of thyrotropin-releasing hormone: specific binding to rat liver membranes.

    abstract::[3H]cyclo(His-Pro) bound with high affinity (59 nM) to a single class of sites in rat liver plasma membranes, without significant tracer degradation during equilibration for 60 min at 0 degrees C. Binding was specific and saturable (3.9 pmol/mg protein), and were increased by the addition of K+, Mg++ and Na+ at optima...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Mori M,Yamada M,Yamaguchi M,Suzuki M,Ohshima K,Kobayashi I,Kobayashi S

    更新日期:1986-01-14 00:00:00

  • Suppression of myostatin with vector-based RNA interference causes a double-muscle effect in transgenic zebrafish.

    abstract::Myostatin belongs to the transforming growth factor (TGF)-beta superfamily and is a potent negative regulator of skeletal muscle development and growth. We utilized microinjection of an antisense RNA-expressing vector to establish a hereditarily stable myostatin gene knockdown zebrafish strain with a double-muscle phe...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lee CY,Hu SY,Gong HY,Chen MH,Lu JK,Wu JL

    更新日期:2009-10-02 00:00:00

  • Increased copper toxicity in Saccharomyces cerevisiae lacking VPS35, a component of the retromer and monogenic Parkinson disease gene in humans.

    abstract::The Saccharomyces cerevisiae gene VPS35 encodes a component of the retromer complex which is involved in vesicle transport from endosomes to the trans-Golgi network. Yeast and human VPS35 orthologs are highly conserved and mutations in human VPS35 cause an autosomal dominant form of late-onset Parkinson disease (PD). ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sowada N,Stiller B,Kubisch C

    更新日期:2016-08-05 00:00:00

  • Comprehensive investigation of aberrant microRNAs expression in cells culture model of MnCl2-induced neurodegenerative disease.

    abstract::Manganese (Mn) is required in various human physiological processes. Excessive Mn exposure causes manganism, a progressive neurodegenerative disorder similar to idiopathic Parkinson's disease (IPD). However, the detailed mechanism of Mn-induced neurotoxicity is not yet fully understood. MicroRNAs (miRNAs) play importa...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: He R,Xie X,Lv L,Huang Y,Xia X,Chen X,Zhang L

    更新日期:2017-04-29 00:00:00

  • Function of the PLZF gene in early development and self-renewal of T cells in mice.

    abstract:BACKGROUND/AIMS:The expression of transcription factor Zbtb1 is essential for the maintenance and development of various blood cells in the hematopoietic system. In the current study, we found that the total number of thymocytes in PLZF deficient mice was reduced compared with thymocytes in wild-type mice, and the numb...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Du JL,Cao X,Liu HY,Zeng Y,Yang XC,Wan XM,Chang FF,Zhao TY,Jia XY,Wang HZ,Liu J,Cai KZ,Ma ZR

    更新日期:2019-04-16 00:00:00