Development-related effects of recombinant activin on steroid synthesis in rat granulosa cells.


:Activin is structurally related to polypeptide growth factors such as transforming-growth factor-beta, which may have paracrine and/or autocrine functions in the ovaries. We have investigated the action of activin on granulosa cell steroidogenesis in vitro in relation to preovulatory follicular development in vivo. Estrogen-primed immature female rats received no other treatment (nondifferentiated granulosa cells), treatment with ovine (o) FSH (differentiated granulosa cells), or treatment with oFSH followed by human (h) CG (preovulatory granulosa cells) to stimulate preovulatory follicular development. Granulosa cells were isolated and cultured in the presence and absence of recombinant human activin-A using serum-free medium supplemented with 1.0 microM testosterone as an aromatase substrate and hFSH, hLH, forskolin, or 8-bromo-cAMP to stimulate steroid synthesis in vitro. After 48 h, medium was collected for measurement of estradiol (aromatase activity), progesterone, and cAMP. Basal steroid synthesis in nondifferentiated granulosa cells was unaffected by activin, but both aromatase activity and progesterone production induced by treatment with FSH in vitro were dosedependently enhanced up to 10-fold by the presence of activin. FSH-stimulated cAMP production was not measurably altered by activin; however, steroidogenesis induced by forskolin or 8-bromo-cAMP was significantly enhanced by the factor. Thus the effect of activin on steroidogenesis includes action at a subcellular level(s) distal to the production of cAMP. After gonadotropin treatment in vivo, granulosa cell aromatase activity and progesterone production showed divergent responses to activin in vitro. Basal-, FSH-, and LH-stimulated aromatase activity were all enhanced by activin in cultures of differentiated and preovulatory granulosa cells. However, whereas basal progesterone production was stimulated by activin in cultures of differentiated granulosa cells, in preovulatory granulosa cells it was inhibited. Moreover, in vitro stimulation of progesterone production by treatment of both differentiated and preovulatory granulosa cells with FSH or LH was suppressed by the presence of activin. Thus rat granulosa cells display development-related steroidogenic responses to activin, aromatase production becoming enhanced and progesterone production suppressed as follicular maturation progresses. These results further implicate activin as a local modulator of granulosa cell steroid synthesis in the ovaries, although its functional significance has yet to be established.






Miró F,Smyth CD,Hillier SG




Has Abstract


1991-12-01 00:00:00












  • The effects of transforming growth factor-beta on growth and differentiation of the continuous rat thyroid follicular cell line, FRTL-5.

    abstract::Transforming growth factor-beta (TGF beta) has been shown to influence the growth and differentiation of many widely varied cell types in vitro, including some that are endocrinologically active. We have investigated the previously unknown effects of this unique growth factor in the differentiated rat thyroid follicul...


    pub_type: 杂志文章


    authors: Morris JC 3rd,Ranganathan G,Hay ID,Nelson RE,Jiang NS

    更新日期:1988-09-01 00:00:00

  • RET/PTC rearrangements and BRAF mutations in thyroid tumorigenesis.

    abstract::Thyroid papillary carcinoma is the most common type of endocrine cancer. It is frequently associated with genetic alterations leading to activation of the MAPK signaling pathway. The two most frequently affected genes, BRAF and RET, are activated by either point mutation or as a result of chromosomal rearrangement. Th...


    pub_type: 杂志文章,评审


    authors: Ciampi R,Nikiforov YE

    更新日期:2007-03-01 00:00:00

  • Comparative therapeutic effects of vitamin D3 and its derivatives on experimental renal osteodystrophy.

    abstract::The comparative effectiveness of vitamin D3 and its derivatives in curing hyperparathyroidism and osteodystrophic bone lesions was examined in a laboratory model of renal osteodystrophy associated with marked secondary hyperparathyroidism in rats. The experimental model was prepared by a single injection of homologous...


    pub_type: 杂志文章


    authors: Fukushima M,Niki R,Ohkawa H,Shimizu T,Matsunaga I,Nakano H,Takagaki Y,Nishii Y,Okano K,Suda T

    更新日期:1980-07-01 00:00:00

  • Parathyroid hormone and calcium: interactions in the control of renin secretion in the isolated, nonfiltering rat kidney.

    abstract::The present study was designed to characterize the interaction of calcium and PTH in the control of renin release in isolated rat kidneys perfused in a closed circuit at constant flow. Kidneys were rendered nonfiltering using low perfusion pressures (70 mm Hg) and a hyperoncotic perfusate (100 g/liter BSA). Under thes...


    pub_type: 杂志文章


    authors: Helwig JJ,Musso MJ,Judes C,Nickols GA

    更新日期:1991-09-01 00:00:00

  • The hepatic extraction of gastric inhibitory polypeptide and insulin.

    abstract::The hepatic extractions of gastric inhibitory polypeptide (GIP) and insulin were determined using in vitro and in vivo methods to assess the role of the liver in GIP metabolism and the possible effect of GIP on the hepatic extraction of insulin. During in vitro studies using the isolated perfused rat liver, infusion o...


    pub_type: 杂志文章


    authors: Hanks JB,Andersen DK,Wise JE,Putnam WS,Meyers WC,Jones RS

    更新日期:1984-09-01 00:00:00

  • Glucocorticoids inhibit keratinocyte growth factor production in primary dermal fibroblasts.

    abstract::The participation of growth factors in wound healing and tissue repair has been well established. Previous studies demonstrated that the expression of keratinocyte growth factor (KGF) was greatly elevated shortly after injury and that topical application of KGF accelerated healing. Steroidal antiinflammatory agents, s...


    pub_type: 杂志文章


    authors: Chedid M,Hoyle JR,Csaky KG,Rubin JS

    更新日期:1996-06-01 00:00:00

  • Genome-Wide Identification of Basic Helix-Loop-Helix and NF-1 Motifs Underlying GR Binding Sites in Male Rat Hippocampus.

    abstract::Glucocorticoids regulate hippocampal function in part by modulating gene expression through the glucocorticoid receptor (GR). GR binding is highly cell type specific, directed to accessible chromatin regions established during tissue differentiation. Distinct classes of GR binding sites are dependent on the activity o...


    pub_type: 杂志文章


    authors: Pooley JR,Flynn BP,Grøntved L,Baek S,Guertin MJ,Kershaw YM,Birnie MT,Pellatt A,Rivers CA,Schiltz RL,Hager GL,Lightman SL,Conway-Campbell BL

    更新日期:2017-05-01 00:00:00

  • Veratridine and ouabain stimulate calcium-dependent prolactin release.

    abstract::We have investigated the effects of veratridine, a Na+ channel activator, and ouabain, an inhibitor of Na+-K+-ATPase, on short term (1-h) PRL release from primary cultures of rat anterior pituitary cells and from the rat anterior pituitary cell line GH4C1 in culture. Both compounds should increase intracellular Na+. V...


    pub_type: 杂志文章


    authors: Scammell JG,Dannies PS

    更新日期:1983-10-01 00:00:00

  • The decidual prolactin receptor and its regulation by decidua-derived factors.

    abstract::Decidualization of the endometrial stroma in the rat gives rise to two different cell populations, located either mesometrially or antimesometrially in the uterus. We have previously shown that the rat decidua is the site of production and action of a PRL-like hormone. In this investigation we examined, using reverse ...


    pub_type: 杂志文章


    authors: Gu Y,Srivastava RK,Clarke DL,Linzer DI,Gibori G

    更新日期:1996-11-01 00:00:00

  • Extension of Survival in Bilaterally Adrenalectomized Mice by Implantation of SF-1/Ad4BP-Induced Steroidogenic Cells.

    abstract::Mesenchymal stroma/stem cells (MSCs) exist in adult tissues, such as adipose tissue and bone marrow, and differentiate into cells of multiple lineages. In previous studies, we found that MSCs differentiate into steroidogenic cells by forced expression of steroidogenic factor 1 (SF-1)/adrenal 4 binding protein (Ad4BP),...


    pub_type: 杂志文章


    authors: Tanaka T,Aoyagi C,Mukai K,Nishimoto K,Kodama S,Yanase T

    更新日期:2020-03-01 00:00:00

  • AP-1 Transcription Factors c-FOS and c-JUN Mediate GnRH-Induced Cadherin-11 Expression and Trophoblast Cell Invasion.

    abstract::GnRH is expressed in first-trimester human placenta and increases cell invasion in extravillous cytotrophoblasts (EVTs). Invasive phenotypes have been reported to be regulated by transcription factor activator protein 1 (AP-1) and mesenchymal cadherin-11. The aim of our study was to investigate the roles of AP-1 compo...


    pub_type: 杂志文章


    authors: Peng B,Zhu H,Ma L,Wang YL,Klausen C,Leung PC

    更新日期:2015-06-01 00:00:00

  • Comparison of inhibitory effects of 3,5,3'-triiodothyronine (T3), thyroxine (T4), 3,3,',5'-triiodothyronine (rT3), and 3,3'-diiodothyronine (T2) on thyrotropin-releasing hormone-induced release of thyrotropin in the rat in vitro.

    abstract::In order to compare, in vitro, the TSH suppressive effects of iodothyronines, rat pituitary quarters were first preincubated with T4, T3, rT3, or 3,3'-diiodothyronine (T2) in Gey and Gey buffer containing 1% bovine serum albumin for 2 h at 37 C and then incubated at 37 C for 1 h with the iodothyronine under study and ...


    pub_type: 杂志文章


    authors: Chopra IJ,Carlson HE,Solomon DH

    更新日期:1978-08-01 00:00:00

  • Interleukin-2 driven nuclear translocation of prolactin in cloned T-lymphocytes.

    abstract::The nuclear translocation of PRL is demonstrated at the immunofluorescence and electron microscopic (EM) levels in interleukin-2 (IL2)-stimulated cloned T-cells and concanavalin A-stimulated splenocytes. This translocation occurs 2-10 h after IL2 stimulation, and is reversed by the addition of anti-PRL antiserum into ...


    pub_type: 杂志文章


    authors: Clevenger CV,Sillman AL,Prystowsky MB

    更新日期:1990-12-01 00:00:00

  • The nuclear receptor corepressor has organizational effects within the developing amygdala on juvenile social play and anxiety-like behavior.

    abstract::Nuclear receptor function on DNA is regulated by the balanced recruitment of coregulatory complexes. Recruited proteins that increase gene expression are called coactivators, and those that decrease gene expression are called corepressors. Little is known about the role of corepressors, such as nuclear receptor corepr...


    pub_type: 杂志文章


    authors: Jessen HM,Kolodkin MH,Bychowski ME,Auger CJ,Auger AP

    更新日期:2010-03-01 00:00:00

  • Sex difference in septal neurons projecting axons to midbrain central gray in rats: a combined double retrograde tracing and ER-immunohistochemical study.

    abstract::Sex difference in the number of neurons projecting axons from the lateral septum (LS) to the midbrain central gray (MCG) that are concerned with the lordosis-inhibiting system was investigated by injection of Fluoro-Gold (FG), a retrograde tracer, into the rostral MCG on the right side in male and female rats. Immunoh...


    pub_type: 杂志文章


    authors: Tsukahara S,Yamanouchi K

    更新日期:2002-01-01 00:00:00

  • Dietary sugar in healthy female primates perturbs oocyte maturation and in vitro preimplantation embryo development.

    abstract::The consumption of refined sugars continues to pose a significant health risk. However, nearly nothing is known about the effects of sugar intake by healthy women on the oocyte or embryo. Using rhesus monkeys, we show that low-dose sucrose intake over a 6-month period has an impact on the oocyte with subsequent effect...


    pub_type: 杂志文章


    authors: Chaffin CL,Latham KE,Mtango NR,Midic U,VandeVoort CA

    更新日期:2014-07-01 00:00:00

  • Disrupted murine leukemia inhibitory factor (LIF) gene attenuates adrenocorticotropic hormone (ACTH) secretion.

    abstract::Leukemia Inhibitory Factor (LIF) and its receptors in human and mouse pituicytes are expressed abundantly in corticotrophs and somatotrophs. As LIF induces POMC transcription and LPS-mediated stress also induces hypothalamic and pituitary LIF expression, we studied ACTH secretion in LIF knockout (LIF KO) mice. Basal A...


    pub_type: 杂志文章


    authors: Akita S,Malkin J,Melmed S

    更新日期:1996-07-01 00:00:00

  • Chronic nicotine exposure enhances insulin-induced mitogenic signaling via up-regulation of alpha7 nicotinic receptors in isolated rat aortic smooth muscle cells.

    abstract::Insulin resistance and smoking are significant risk factors for cardiac and cerebral vascular diseases. Because vascular smooth muscle cells play a key role in the development and progression of atherosclerosis, we investigated the effect of nicotine on insulin-induced mitogenic signaling in aortic vascular smooth mus...


    pub_type: 杂志文章


    authors: Wada T,Naito M,Kenmochi H,Tsuneki H,Sasaoka T

    更新日期:2007-02-01 00:00:00

  • Failure of growth hormone (GH) to feed back at the level of the pituitary to alter the response of the somatotrophs to GH-releasing factor.

    abstract::GH feeds back at the level of the central nervous system to alter the release of somatostatin and GRF, resulting in altered GH release. The purpose of this study was to see whether the concentration of GH, impinging directly on the somatotrophs of the adenohypophysis, would alter the responsiveness of the somatotrophs...


    pub_type: 杂志文章


    authors: Kraicer J,Lussier B,Moor BC,Cowan JS

    更新日期:1988-04-01 00:00:00

  • Estrogen sulfotransferase: discrete and androgen-dependent expression in the male reproductive tract and demonstration of an in vivo function in the mouse epididymis.

    abstract::Estrogen sulfotransferase (EST) catalyzes the sulfoconjugation and inactivation of the steroid hormone estrogen. It is known previously that EST is expressed abundantly in Leydig cells of the testis. We recently have shown that male mice with targeted EST gene disruption developed age related Leydig cell and seminifer...


    pub_type: 杂志文章


    authors: Tong MH,Song WC

    更新日期:2002-08-01 00:00:00

  • Temporal silencing of an androgenic gland-specific insulin-like gene affecting phenotypical gender differences and spermatogenesis.

    abstract::Androgenic glands (AGs) of the freshwater prawn Macrobrachium rosenbergii were subjected to endocrine manipulation, causing them to hypertrophy. Transcripts from these glands were used in the construction of an AG cDNA subtractive library. Screening of the library revealed an AG-specific gene, termed the M. rosenbergi...


    pub_type: 杂志文章


    authors: Ventura T,Manor R,Aflalo ED,Weil S,Raviv S,Glazer L,Sagi A

    更新日期:2009-03-01 00:00:00

  • The Intermediate lactotroph: a morphologically distinct, ghrelin-responsive pituitary cell in the dwarf (dw/dw) rat.

    abstract::Profound somatotroph hypoplasia in the dwarf (dw/dw) rat is accompanied by an estrogen-dependent induction of prolactin secretion by the GH secretagogue, GHRP-6. Using electron microscopy, we demonstrated that the reduction in the somatotroph population in the dw/dw pituitary is accompanied by the presence of a morpho...


    pub_type: 杂志文章


    authors: Huerta-Ocampo I,Christian HC,Thompson NM,El-Kasti MM,Wells T

    更新日期:2005-11-01 00:00:00

  • Differential regulation of progestin receptor immunoreactivity in the rabbit oviduct.

    abstract::Cellular distribution of progestin receptor immunoreactivity (PR-IR) in paraffin-embedded rabbit oviduct tissue was determined using a monoclonal antibody and the indirect immunoperoxidase method. Ampullar and isthmic tissue from ovariectomized animals primed with 17 beta-estradiol or vehicle alone was examined. As ex...


    pub_type: 杂志文章


    authors: Hyde BA,Blaustein JD,Black DL

    更新日期:1989-09-01 00:00:00

  • Effect of ACTH, alpha-MSH, and beta-Lipotropin on calcium and phosphorus metabolism in the rabbit.

    abstract::Studies were undertaken to investigate the effects of synthetic 1-24 adrenocorticotropin (ACTH), bovine alpha melanocyte-stimulating hormone (alpha-MSH), and ovine beta lipotropin (beta-LPH) on plasma calcium and phosphate in rabbits. Equimolar concentrations of these hormones were infused intravenously in intact and ...


    pub_type: 杂志文章


    authors: Gildersleeve DL,Pearson TA,Baghdiantz A,Foster GV

    更新日期:1975-12-01 00:00:00

  • Axonal protection by 17β-estradiol through thioredoxin-1 in tumor necrosis factor-induced optic neuropathy.

    abstract::Axonal degeneration often leads to the death of neuronal cell bodies. Previous studies demonstrated the substantial protective role of 17β-estradiol (E2) in several types of neuron. However, most studies examined cell body protection, and the role of 17β-E2 in axonal degeneration of retinal ganglion cells (RGC) remain...


    pub_type: 杂志文章


    authors: Kitaoka Y,Munemasa Y,Hayashi Y,Kuribayashi J,Koseki N,Kojima K,Kumai T,Ueno S

    更新日期:2011-07-01 00:00:00

  • Localization of olfactory cyclic nucleotide-gated channels in rat gonadotropin-releasing hormone neurons.

    abstract::The GT1 GnRH cell lines express all three subunits of the cyclic nucleotide-gated (CNG) channels (CNG2, 4.3 and 5) expressed in olfactory neurons. We investigated using in situ hybridization and double immunofluorescence whether endogenous GnRH neurons in therat also express CNG channel subunits. Sections from male an...


    pub_type: 杂志文章


    authors: El-Majdoubi M,Weiner RI

    更新日期:2002-06-01 00:00:00

  • Effect of K+ deprivation and ouabain upon glucagon release.

    abstract::The effects of K+ depletion and ouabain (0.1, 0.5, and 1 mM) on glucagon and insulin release from the in vitro perfused rat pancreas were studied in the absence or presence of arginine (10 mM). In the absence of arginine, K+ depletion resulted in an inhibition of glucagon release, both at a low (2.8 mM) and a higher (...


    pub_type: 杂志文章


    authors: Leclercq-Meyer V,Marchand J,Malaisse WJ

    更新日期:1983-07-01 00:00:00

  • Genetic control of ductal morphology, estrogen-induced ductal growth, and gene expression in female mouse mammary gland.

    abstract::The uterotropic response of the uterus to 17β-estradiol (E2) is genetically controlled, with marked variation observed depending on the mouse strain studied. Previous genetic studies from our laboratory using inbred mice that are high (C57BL6/J; B6) or low (C3H/HeJ; C3H) responders to E2 led to the identification of q...


    pub_type: 杂志文章


    authors: Wall EH,Case LK,Hewitt SC,Nguyen-Vu T,Candelaria NR,Teuscher C,Lin CY

    更新日期:2014-08-01 00:00:00

  • Different regulation of growth hormone-releasing factor-sensitive adenylate cyclase in the anterior pituitary of young and aged rats.

    abstract::Low basal GH secretion and reduced GH responsiveness to different GH secretagogues, including GHRF, have been reported in aged animals and humans. Parallel to the in vivo findings, an impaired GH responsiveness to GHRF is evident in somatotropes from old rats of either sex. We report here that in anterior pituitaries ...


    pub_type: 杂志文章


    authors: Parenti M,Dall'ara A,Rusconi L,Cocchi D,Müller EE

    更新日期:1987-11-01 00:00:00

  • Estrogen acidifies vaginal pH by up-regulation of proton secretion via the apical membrane of vaginal-ectocervical epithelial cells.

    abstract::The objective of this study was to assess estrogen-dependent cellular mechanisms that could contribute to the acid pH of the vaginal lumen. Cultures of normal human cervical-vaginal epithelial (hECE) cells and endocervical cells were grown on filters, and acidification of the extracellular solutions on the luminal (L-...


    pub_type: 杂志文章


    authors: Gorodeski GI,Hopfer U,Liu CC,Margles E

    更新日期:2005-02-01 00:00:00