Abstract:
:Pituitary growth hormone (GH) and insulinlike growth factor (IGF)-1 are anabolic hormones whose physiological roles are particularly important during development. The activity of the GH/IGF-1 axis is controlled by complex neuroendocrine systems including two hypothalamic neuropeptides, GH-releasing hormone (GHRH) and somatostatin (SRIF), and a gastrointestinal hormone, ghrelin. The neurotransmitter acetylcholine (ACh) is involved in tuning GH secretion, and its GH-stimulatory action has mainly been shown in adults but is not clearly documented during development. ACh, together with these hormones and their receptors, is expressed before birth, and somatotroph cells are already responsive to GHRH, SRIF, and ghrelin. We thus hypothesized that ACh could contribute to the modulation of the main components of the somatotropic axis during development. In this study, we generated a choline acetyltransferase knockout mouse line and showed that heterozygous mice display a transient deficit in ACh from embryonic day 18.5 to postnatal day 10, and they recover normal ACh levels from the second postnatal week. This developmental ACh deficiency had no major impact on weight gain and cardiorespiratory status of newborn mice. Using this mouse model, we found that endogenous ACh levels determined the concentrations of circulating GH and IGF-1 at embryonic and postnatal stages. In particular, serum GH level was correlated with brain ACh content. ACh also modulated the levels of GHRH and SRIF in the hypothalamus and ghrelin in the stomach, and it affected the levels of these hormones in the circulation. This study identifies ACh as a potential regulator of the somatotropic axis during the developmental period.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Lecomte MJ,Bertolus C,Ramanantsoa N,Saurini F,Callebert J,Sénamaud-Beaufort C,Ringot M,Bourgeois T,Matrot B,Collet C,Nardelli J,Mallet J,Vodjdani G,Gallego J,Launay JM,Berrard Sdoi
10.1210/en.2017-03175subject
Has Abstractpub_date
2018-04-01 00:00:00pages
1844-1859issue
4eissn
0013-7227issn
1945-7170pii
4917524journal_volume
159pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::We have previously reported that the acute injection of interleukin-1 beta (IL-1 beta) into the brain ventricles of intact female rats promptly decreases LHRH release and inhibits gene expression of this peptide in the medial preoptic area (MPOA). The present studies were therefore designed to determine whether contin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.6.8243260
更新日期:1993-12-01 00:00:00
abstract::During the reproductive cycle, fluctuations in circulating estrogens affect multiple homeostatic systems controlled by hypothalamic neurons. Two of these neuronal populations are arcuate proopiomelanocortin and neuropeptide Y neurons, which control energy homeostasis and feeding. Estradiol modulates these neurons eith...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0769
更新日期:2008-12-01 00:00:00
abstract::We used differential display PCR on two GnRH producing cell lines to identify genes involved in GnRH gene expression and neuronal migration. RNA from Gn10 cells (derived from a tumor in the olfactory area when GnRH neurons are migrating and make low levels of GnRH) and from GT1-7 cells (derived from a tumor in forebra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.8.6221
更新日期:1998-08-01 00:00:00
abstract::Studies in rodents demonstrate that the mitogen, IGF-I, stimulates intestinal peptide YY (PYY) expression. To investigate whether the stimulatory influence of IGF-I is exerted at the level of gene transcription, rat PYY 5'-upstream sequences (-2800/+37 bp, -770/+37 bp, -127/+37 bp) fused to the firefly luciferase (luc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0770
更新日期:2004-02-01 00:00:00
abstract::Galanin-like peptide (GALP), a 29-amino-acid neuropeptide, is located in the hypothalamic arcuate nucleus (ARC), binds to galanin receptor subtype 2, and induces food intake upon intracerebroventricular (icv) injection in rats. However, neural mechanisms underlying its orexigenic action remain unclear. We aimed to ide...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0907
更新日期:2006-04-01 00:00:00
abstract::Angiotensin II receptors regulate muscle microvascular recruitment and the delivery of nutrients, oxygen, and insulin to muscle. Although angiotensin type 1 receptor antagonism increases muscle microvascular perfusion and insulin action, angiotensin type 2 receptor blockade markedly restricts muscle microvascular bloo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00585
更新日期:2018-02-01 00:00:00
abstract::Insulin and insulin-like growth factors (IGFs) stimulate responses in skeletal muscle that include effects on carbohydrate and fat metabolism, protein turnover, growth, and differentiation. To gain insight into the relative importance of insulin and IGFs at different stages of development, the expression of their spec...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-2-1064
更新日期:1989-02-01 00:00:00
abstract::Multiple interferon (IFN)-tau genes exist in cattle, but it has remained unclear how many are expressed, the extent of their variation, and whether different genes exhibit similar patterns of expression and code for proteins with similar biological activities. A total of 118 complementary DNA (cDNA) were bi-directiona...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.7.8249
更新日期:2001-07-01 00:00:00
abstract::The mechanisms by which oxytocin (OT) stimulates extracellular signal-regulated kinase 1/2 (ERK1/2) are only partially understood. OT receptor (OTR) signals predominantly through Galpha(q), but ERK1/2 phosphorylation (ERK1/2-P) in PHM1 myometrial cells was not eliminated by inhibition of downstream effectors such as p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-221039
更新日期:2003-07-01 00:00:00
abstract::Developmental exposures to phthalates are suspected to contribute to risk of metabolic syndrome. However, findings from human studies are inconsistent, and long-term metabolic impacts of early-life phthalate and phthalate mixture exposures are not fully understood. Furthermore, most animal studies investigating metabo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2019-00287
更新日期:2019-07-01 00:00:00
abstract::Although reovirus infection may lead to changes in endocrine function in vivo, little is known about the precise interaction of reovirus with endocrine cells. In this study we have examined the effects of reovirus infection on two types of endocrine cells, GH4C1 cells and RINm5F cells. Both type 1 reovirus and type 3 ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-6-2430
更新日期:1985-06-01 00:00:00
abstract::The genetically obese Zucker rat is a widely used model of early-onset obesity. Like obese children, these obese rats are hyperinsulinemic and have low GH secretion. However, data on linear growth and insulin-like growth factor-I (IGF-I) levels in this model are scanty and contradictory. In the present study, we inves...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.3.7509740
更新日期:1994-03-01 00:00:00
abstract::Calcitonin gene-related peptide (CGRP)-I has been reported to inhibit gastric acid secretion through stimulation of gastric somatostatin-14 (S-14) release. To establish whether some of the effects of CGRP-I on intestinal function might also be mediated through somatostatin, fetal rat intestinal cultures were treated w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.6.7902270
更新日期:1993-12-01 00:00:00
abstract::Multiplication-stimulating activity (MSA), an insulin-like growth factor (IGF) (rat IGF II), binds to extracts of many tissues from fetal lambs. We now report the presence of high concentrations of a glycoprotein receptor with a high affinity for MSA-II in microsomes prepared from fetal lamb liver. The binding of radi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-3-982
更新日期:1985-09-01 00:00:00
abstract::Hypothalamic peninsulae, posteriorly continuous with the brain stem, were produced in ovariectomized rhesus monkeys by aspiration of the cerebral hemispheres and all brain structures anterior and dorsal to the optic chiasm. These preparations were sustained for 52 to 60 h postoperatively by maintaining blood gases and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-101-4-1264
更新日期:1977-10-01 00:00:00
abstract::The ability of a monoclonal antiidiotypic antibody (clone 1D5) directed against the binding site of a monoclonal antiestradiol antibody to interact with the estrogen receptor (ER) was investigated. The following lines of evidence indicate that clone 1D5 has the capacity of mimicking the actions of estradiol, and recog...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.6.1597160
更新日期:1992-06-01 00:00:00
abstract::It has been reported that the endocannabinoid anandamide (AEA) exerts an adverse effect on human sperm motility, which has been ascribed to inhibition of mitochondrial activity. This seems to be at variance with evidence suggesting a major role of glycolysis in supplying ATP for sperm motility; furthermore, the role o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0484
更新日期:2010-12-01 00:00:00
abstract::Complementary DNA-encoding proteins with high homology to steroidogenic acute regulatory proteins (StAR) of mammals were cloned from rainbow trout head kidney and a mixture of several brook trout tissues. A cDNA encoding an MLN64 homolog was also cloned from brook trout. The C-terminal domains of rainbow trout StAR an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.6.8672
更新日期:2002-06-01 00:00:00
abstract::In mammals, lactation is associated with a period of infertility characterized by the loss of pulsatile secretion of GnRH and cessation of ovulatory cycles. Despite the importance of lactational infertility in determining overall fecundity of a species, the mechanisms by which the suckling stimulus suppresses GnRH sec...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1621
更新日期:2014-01-01 00:00:00
abstract::Sertoli cell intracellular protein 1 (SCc1) and 2 (SCc2) are polypeptides found in rat Sertoli cell cultures incubated with either FSH or (Bu)2cAMP. They were first identified in [35S]methionine-labeled Sertoli cell lysates using two-dimensional gel electrophoresis. Here we extend these observations by showing that SC...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.5.1330490
更新日期:1992-11-01 00:00:00
abstract::In previous work we showed that the chondrodysplastic phenotype of mice homozygous for a null mutation of the PTH-related peptide (PTHrP) gene was due in part to reduced proliferation and aberrant differentiation of growth plate chondrocytes. In the present study we have extended those observations by examining chondr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.11.8895380
更新日期:1996-11-01 00:00:00
abstract::The hepatic extractions of gastric inhibitory polypeptide (GIP) and insulin were determined using in vitro and in vivo methods to assess the role of the liver in GIP metabolism and the possible effect of GIP on the hepatic extraction of insulin. During in vitro studies using the isolated perfused rat liver, infusion o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-3-1011
更新日期:1984-09-01 00:00:00
abstract::Adenylate cyclase of rat renal cortex was inhibited by angiotensin II (AII). Inhibition required Na+ (100-200 mM) and GTP (10(-8)-10(-4) M) and was opposed by the receptor antagonist [1-sarcosine, 8-isoleucine]AII. The EC50 value (+/- SE)for inhibition by AII was 3.7 +/- 1.2 nM, and the maximum inhibition (+/- SE) was...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-5-1687
更新日期:1982-11-01 00:00:00
abstract::Rat sexual maturation is preceded by a reduction of the interpulse interval (IPI) of GnRH neurosecretion. This work aims at studying disruption of that neuroendocrine event in females after early exposure to a very low dose of bisphenol A (BPA), a ubiquitous endocrine disrupting chemical. Female rats were exposed to v...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1937
更新日期:2016-05-01 00:00:00
abstract::This study investigated the relationship between plasma gonadotropin concentrations and the initiation and maintenance of preovulatory follicular growth in macaques. Eight adult cynomolgus monkeys were treated with a GnRH antagonist [AcD2Nal1-4ClDPhe2, DTrp3, DArg6, DAla10]GnRH X HOAc to block endogenous gonadotropin ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-5-2025
更新日期:1986-11-01 00:00:00
abstract::GnRH neurons are regulated by hypothalamic kisspeptin neurons. Recently, galanin was identified in a subpopulation of kisspeptin neurons. Although the literature thoroughly describes kisspeptin activation of GnRH neurons, little is known about the effects of galanin on GnRH neurons. This study investigated whether gal...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1064
更新日期:2016-08-01 00:00:00
abstract::Intravascular injections of adrenocorticotropic hormone in hypophysectomized rats were approximately 3 times more effective in stimulating increases in plasma corticosteroid concentration when given at the offset rather than the onset of the daily photoperiod (12 h:12 h light-dark cycle). Reversal of the photoperiodic...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-5-1875
更新日期:1978-11-01 00:00:00
abstract::Pure porcine cholecystokinin-33 [the triacontatriapeptide form of cholecystokinin (CCK-33)], gastric inhibitory polypeptide (GIP), and secretin were infused in rats in doses of 1, 10 and 1000 pmol/kg . min. The peptides were administered alone or in combination with glucose (40 mg/kg . min) or arginine (50 mg/kg . min...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-4-1268
更新日期:1982-04-01 00:00:00
abstract::To ascertain if reductions in fetal plasma cortisol cause increases in fetal plasma ACTH, we treated pregnant ewes or their fetuses with aminoglutethimide (10 mg/kg BW) and metyrapone (20 mg/kg BW) and measured the hormonal responses with RIAs. When given to fetuses (n = 9) at 0.90 +/- 0.01 gestation (term-145 days), ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-3-1307
更新日期:1988-09-01 00:00:00
abstract::We previously reported that hormones important for the normal growth and function of FRTL-5 rat thyroid cells, TSH, or its cAMP signal plus insulin or IGF-I, could transcriptionally suppress constitutive and gamma-interferon (IFN)-increased synthesis of the 90K protein (also known as Mac-2BP). Here we cloned the 5'-fl...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0024
更新日期:2007-07-01 00:00:00