Abstract:
:Downstream of Ras, the serine/threonine kinase B-raf has been reported to be mutated, amongst other carcinomas, in a substantial subset of primary melanomas, with a preponderance of mutations within the kinase domain, including the activating V599E and V599K transitions. We investigated a representative series of 54 resection specimens of melanoma lymph node metastases for the presence of mutations within the activation segment (exon 15) of the B-raf kinase domain by polymerase chain reaction (PCR) and single-strand conformational polymorphism (SSCP) gel electrophoresis. Sequencing of cloned PCR-SSCP amplicons resulted in 24 (44%) samples harbouring somatic mutations, which is not significantly different from the mutation frequency found in recently investigated primary cutaneous melanomas (Deichmann M, Thome M, Benner A, Näher H. B-raf exon 15 mutations are common in primary melanoma resection specimens but not associated with clinical outcome. Oncology 2004; 66:411-419). The activating mutation T1796A was present in 20 (83%) of these resection specimens, followed in frequency by the oncogenic g1795A mutation in five (21%) cases. With regard to the B-raf protein sequence, the acidic amino acid transitions V599E and V599K were predicted in 15 (62%) and five (21%) of the 24 positive metastases, respectively. The detection of mutations at this hot spot codon was significantly associated with subsequent visceral metastasis (P=0.03; Fisher's exact test). During the transition from primary melanomas (see reference above) to lymph node metastases, the spectrum of B-raf mutations narrows significantly (P=0.00047). The oncogenic B-raf mutations V599E and V599K, as early events in melanocyte transformation, persist throughout metastasis with important prognostic implications.
journal_name
Melanoma Resjournal_title
Melanoma researchauthors
Kirschner M,Helmke B,Starz H,Benner A,Thome M,Deichmann Mdoi
10.1097/00008390-200510000-00011subject
Has Abstractpub_date
2005-10-01 00:00:00pages
427-34issue
5eissn
0960-8931issn
1473-5636pii
00008390-200510000-00011journal_volume
15pub_type
杂志文章abstract::When cancer cells, including melanoma cells, are genetically altered to secrete cytokines, irradiated and injected into subjects, long-term antitumour immunity is induced. Optimally, existing melanomas induced to produce cytokines in vivo could stimulate this same immune response. Although in vivo electroporation enha...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200012000-00010
更新日期:2000-12-01 00:00:00
abstract::M30 and M65 are relatively new assays that detect different circulating forms of the epithelial cell structural protein cytokeratin 18. This study was carried out to investigate the serum levels of M30 and M65 in patients with melanoma and the relationship with tumor progression and known prognostic parameters. Fifty-...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e328363e4ab
更新日期:2013-10-01 00:00:00
abstract::To date, no study of melanoma patients who have undergone delayed lymph node dissection (DLND) has focused on the independent prognostic factors of overall survival, as calculated from surgery on the primary. Using Kaplan-Meier estimates and Cox's proportional hazard model, the significance of prognostic factors was e...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200010000-00011
更新日期:2000-10-01 00:00:00
abstract::The aim of this study was to determine the impact of complete response (CR) to systemic therapy on survival. We reviewed the cases of 647 chemo-naive patients with metastatic melanoma who were treated with cisplatin-vinblastine-dacarbazine or cisplatin-taxol-dacarbazine alone, or cisplatin-vinblastine-dacarbazine toge...
journal_title:Melanoma research
pub_type: 杂志文章,评审
doi:10.1097/CMR.0b013e328341445f
更新日期:2011-02-01 00:00:00
abstract::Protein tyrosyl phosphorylation is an essential component in intracellular signalling, with diverse and crucial functions including mediation of cell proliferation, survival, death, differentiation, migration and attachment. It is regulated by the balance between the activities of protein tyrosine kinases (PTKs) and p...
journal_title:Melanoma research
pub_type: 杂志文章,评审
doi:10.1097/00008390-200010000-00001
更新日期:2000-10-01 00:00:00
abstract::Significant advances have been made in the treatment of melanoma by targeting key cellular pathways, but additional targets are needed as many patients do not respond or relapse with resistant disease. MicroRNA-155 (MiR-155) has previously been shown to regulate melanoma cell growth and acts as a tumor suppressor. We ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000545
更新日期:2019-04-01 00:00:00
abstract::Immunosuppressed patients are at increased risk of skin cancer. A 67-year-old renal transplant recipient developed a nodular malignant melanoma after 30 years of immunosuppression with azathioprine and prednisolone. The patient died of metastatic disease 3 months after the diagnosis was made. The function of the renal...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e32831bc44a
更新日期:2009-08-01 00:00:00
abstract::In an attempt to define the role of plasminogen activator in invasiveness and differentiation of human melanoma cells, the modulation of these parameters was studied in two melanoma clones characterized by marked differences in their basal features, using 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and retinoic acid, ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199408000-00007
更新日期:1994-08-01 00:00:00
abstract::Pigmented basal cell carcinoma (PBCC) is a tumour with distinct clinical features which occasionally may be difficult to differentiate from malignant melanoma (MM). The purpose of this study was to re-examine the epiluminescence microscopy (ELM) criteria for PBCC and to determine their statistical significance in the ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199708000-00005
更新日期:1997-08-01 00:00:00
abstract::The presence or absence of melanoma cells in human peripheral blood has recently been shown to be associated with disease prognosis, including overall survival. The detection of tyrosinase mRNA-positive circulating melanoma cells by reverse transcription-polymerase chain reaction (RT-PCR) has been limited to dissemina...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200008000-00007
更新日期:2000-08-01 00:00:00
abstract::Prognostic blood biomarkers for patients with uveal melanoma have not been identified. Tumor monosomy-3 is strongly associated with the development of metastatic disease. Tumor expression of human leukocyte antigen class I molecules and insulin-like growth factor (IGF)-1 receptor has also been associated with the deve...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e32835b7154
更新日期:2013-02-01 00:00:00
abstract::The aim of the study was to identify the frequency of asymptomatic brain metastases detected by computed tomography (CT) scans in patients with metastatic cutaneous melanoma referred to first-line systemic treatment. Between 1995 and 2009, 697 Danish patients were screened with a contrast-enhanced CT scan of the brain...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e32835ae915
更新日期:2013-02-01 00:00:00
abstract::MAGE proteins have been identified as potential specific targets for cancer vaccination. Although MAGE-6 and MAGE-12 were originally identified in malignant melanoma there are no studies reporting the frequency of expression of these antigens in this malignancy. These are of relevance particularly for MAGE-6 as recent...
journal_title:Melanoma research
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract::This study addressed the impact of exposure to ultraviolet radiation on the risk of cutaneous malignant melanoma (CMM), as well as the behavioral components at stake in its occurrence. We performed a one-to-one unmatched case-control study among subjects aged 20 years or more with naturally non-pigmented skin in Germa...
journal_title:Melanoma research
pub_type: 杂志文章,多中心研究
doi:10.1097/00008390-199404000-00001
更新日期:1994-04-01 00:00:00
abstract::The proto-oncogene c-Kit encodes a membrane receptor protein with intrinsic tyrosine kinase activity. Activation of c-Kit induces cell proliferation, differentiation or migration among different cell types. The present study provides evidence that c-Kit plays an important role in the cell differentiation rather than i...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199510000-00002
更新日期:1995-10-01 00:00:00
abstract::A large body of evidence in the scientific literature suggests that the numbers of common and atypical nevi are strong, independent risk factors for the occurrence of cutaneous malignant melanoma. Furthermore, some studies recently found an association between high nevus counts and an improved melanoma prognosis. The ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000579
更新日期:2020-04-01 00:00:00
abstract::Glutathione (GSH) is the major non-protein thiol in cells that plays a critical role against damage from electrophilic agents such as alkylating drugs. Selective therapeutic GSH elevation in normal but not in tumour cells has been suggested as a means of protecting host tissues against more intense doses of chemothera...
journal_title:Melanoma research
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract::Melanoma is the deadliest form of skin cancer. Ipilimumab, a novel immunotherapy, is the first treatment shown to improve survival in patients with metastatic melanoma in large randomized controlled studies. The most concerning side effects reported in clinical studies of ipilimumab fall into the category of immune-re...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e32835c7e68
更新日期:2013-02-01 00:00:00
abstract::To investigate the diagnostic value of ultrasound-guided core needle biopsy (US-CNB) in suspected cases of lymph node metastasis from cutaneous melanoma. All patients with cutaneous melanoma followed in Saint-Louis Hospital between 2006 and 2010 who underwent US-CNB for suspicion of melanoma lymph node metastasis were...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000161
更新日期:2015-12-01 00:00:00
abstract::Metastasis is a complicated multi-step process involving interactions between tumour cells, the extracellular matrix and the vessel walls. Experimental observations suggest that leucocyte migration and function could be a suitable model in order to understand tumour cell dissemination. In the present report we show an...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199810000-00001
更新日期:1998-10-01 00:00:00
abstract::Metabolic imaging with F-18 fluorodeoxy-D-glucose positron emission tomography is one of the most sensitive and non-invasive techniques, and has proved useful in melanoma. We designed, in 2004, at the Institute Gustave Roussy, a prospective study to determine the value of F-18 fluorodeoxy-D-glucose positron emission t...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e32815c10b0
更新日期:2007-06-01 00:00:00
abstract::The correlation between ultraviolet radiation of the skin and melanoma incidence in humans is well established. Interestingly, epidemiologic data suggest also a correlation to an increased BMI pointing to metabolic trigger factors in melanoma pathogenesis. To substantiate this connection, we studied the expression of ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000445
更新日期:2018-08-01 00:00:00
abstract::In a previous large randomized, open-label study, retrospective subset analysis revealed that the addition of the Bcl-2 antisense oligonucleotide oblimersen to dacarbazine (Dac) significantly improved overall survival, progression-free survival, and the response rate in chemotherapy-naive patients with advanced melano...
journal_title:Melanoma research
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1097/CMR.0000000000000056
更新日期:2014-06-01 00:00:00
abstract::Electrochemotherapy (ECT), chemotherapy administered in combination with electric fields, has the potential to be an effective localized treatment for cutaneous malignancies. Bleomycin's cytotoxicity was enhanced by exposing tumour cells to electrical fields following intravenous injection of the chemotherapeutic agen...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199702000-00003
更新日期:1997-02-01 00:00:00
abstract::Dissemination of uveal melanomas is almost exclusively haematogenous, making angiogenesis of the tumour a prerequisite for the formation of metastases. Uveal melanomas must employ strategies to evade the immune system in order to escape immune surveillance. We therefore determined the expression of the following angio...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199910000-00003
更新日期:1999-10-01 00:00:00
abstract::Melanoma patients frequently have elevated serum levels of interleukin-6 (IL-6), which is correlated with a poor prognosis. IL-6 activates STAT3 phosphorylation, inducing the transcription of genes that regulate tumor cell proliferation and antiapoptosis. In addition, recent evidence suggests that IL-6 induces the epi...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000021
更新日期:2013-12-01 00:00:00
abstract::Patients with melanoma may experience a variety of different vision symptoms, in part associated with melanoma-associated retinopathy. For several melanoma patients with or without melanoma-associated retinopathy, colour vision deficiencies, especially involving the tritan system, have been reported. The frequency of ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/01.cmr.0000222599.35062.84
更新日期:2006-10-01 00:00:00
abstract::Treatment of patients with metastatic uveal melanoma is very challenging because the tumor commonly spreads to the liver, surgical resection of metastases is rarely possible, and there is no standard effective systemic therapy. We report the case of a 38-year-old man, who presented with metastatic involvement of the l...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e328344958c
更新日期:2011-04-01 00:00:00
abstract::Loss of expression of E-cadherin, the major cell-cell adhesion receptor on keratinocytes, has been linked to tumour progression in various carcinomas. As E-cadherin has been reported to be expressed in cultured human melanocytes, we questioned whether loss of E-cadherin expression may also be related to melanocytic tu...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199604000-00007
更新日期:1996-04-01 00:00:00
abstract::Several comparative genomic hybridization studies provide evidence for overrepresentation of the long arm of chromosome 20 in malignant melanoma. These studies also suggest that chromosome 20q contains genes that may contribute to melanoma pathogenesis. To refine the region of 20q amplification and to identify potenti...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e3280141617
更新日期:2007-02-01 00:00:00