Abstract:
:Melanoma patients frequently have elevated serum levels of interleukin-6 (IL-6), which is correlated with a poor prognosis. IL-6 activates STAT3 phosphorylation, inducing the transcription of genes that regulate tumor cell proliferation and antiapoptosis. In addition, recent evidence suggests that IL-6 induces the epithelial-to-mesenchymal transition and enhances the invasiveness of tumor cells of epithelial origin. However, it is unknown whether IL-6 affects mesenchymal tumor cells. In this study, we examined the effects of IL-6 on melanoma cells and found that IL-6 can enhance their metastatic potential by regulating the expression of Twist and N-cadherin. First, we confirmed that human melanoma tissues express IL-6 (especially at the lesion site), the IL-6 receptor, N-cadherin, and nuclear Twist. Next, we found that IL-6 induces STAT3 phosphorylation in WM-266-4 human melanoma cells, resulting in transient upregulation of Twist, which is a key regulator of metastasis. Importantly, the expression of N-cadherin, a protein downstream of Twist, was also increased on the cell surface after treatment with IL-6. These cells showed enhanced invasiveness, assessed using an invasion assay, and formed more metastatic nodules in the lungs of NOD-SCID mice after an intravenous injection. Importantly, melanoma cells with knocked-down N-cadherin formed less lung nodules compared with control in the NOD-SCID mouse model. Our data suggest that increased serum IL-6 in cancer patients could increase the invasiveness of melanoma cells and accelerate metastasis. Blocking IL-6 in the melanoma microenvironment may therefore inhibit disease progression.
journal_name
Melanoma Resjournal_title
Melanoma researchauthors
Na YR,Lee JS,Lee SJ,Seok SHdoi
10.1097/CMR.0000000000000021subject
Has Abstractpub_date
2013-12-01 00:00:00pages
434-43issue
6eissn
0960-8931issn
1473-5636journal_volume
23pub_type
杂志文章abstract::Cell clones derived from a human melanoma metastasis selected for different integrin profiles were examined in vitro for invasive potential and biological and biochemical features potentially related to this process. Clones which expressed high levels of integrins showed high invasive potential, extracellular matrix d...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199212000-00012
更新日期:1992-12-01 00:00:00
abstract::The objective of this study was to identify novel tumor-suppressor genes in melanoma, using an integrative genomic approach. Data from: (i) earlier reports of DNA loss and gain in malignant melanoma accompanied by comparative genomic hybridization high-definition array data of the entire human genome; (ii) microarray ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e328344a003
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abstract::Conjunctival melanoma is a rare disease, and little is known about its molecular background. Here, we present the case of a 48-year-old patient with conjunctival melanoma and metachronic lymph node and skin metastasis with KRAS p.K117Y mutation in exon 4 in all the lesions. The cancer genome interpreter predicted this...
journal_title:Melanoma research
pub_type: 杂志文章,评审
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更新日期:2018-04-01 00:00:00
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journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200208000-00003
更新日期:2002-08-01 00:00:00
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journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e32835ae915
更新日期:2013-02-01 00:00:00
abstract::Anorectal malignant melanoma is an uncommon tumour. Unlike for cutaneous melanoma, there are few guidelines for its optimal management. In particular, very few palliative treatment strategies have been described for patients with advanced disease. We report on an 80 year old patient with locally advanced anorectal mel...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200208000-00013
更新日期:2002-08-01 00:00:00
abstract::A 58-year-old man with indolent metastatic BRAF mutant melanoma presented with several days' history of progressive ataxia and dysdiadochokinesia. His PET/computed tomography restaging scan indicated two new fluorine-18-fluorodeoxyglucose-avid mesenteric lymph nodes. Meanwhile, his MRI brain and whole spine were withi...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000264
更新日期:2016-08-01 00:00:00
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journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200310000-00007
更新日期:2003-10-01 00:00:00
abstract::Isolated limb perfusion (ILP) with melphalan is used to treat recurrent melanoma. This study aimed to develop a microdialysis technique for melphalan tissue concentration measurement during ILP. The effects of melphalan concentration (50-600 microg/ml), microdIalysis flow rate (0.55-17.5 microl/min), probe length (5-5...
journal_title:Melanoma research
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
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journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e328334c36e
更新日期:2010-06-01 00:00:00
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journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199207000-00006
更新日期:1992-07-01 00:00:00
abstract::Conditional survival in patients with localized primary cutaneous malignant melanoma (CMM) is well described. However, conditional recurrence-free survival (RFS) has not been investigated before. The aim of this study was to determine conditional RFS and test for time dependency in prognostic factors in patients with ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000479
更新日期:2018-12-01 00:00:00
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journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200110000-00002
更新日期:2001-10-01 00:00:00
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journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199211000-00002
更新日期:1992-11-01 00:00:00
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journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000625
更新日期:2020-02-01 00:00:00
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journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e328329ae49
更新日期:2009-04-01 00:00:00
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journal_title:Melanoma research
pub_type: 临床试验,杂志文章,多中心研究
doi:
更新日期:1993-04-01 00:00:00
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journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e32833e3286
更新日期:2010-12-01 00:00:00
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journal_title:Melanoma research
pub_type: 临床试验,杂志文章
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journal_title:Melanoma research
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journal_title:Melanoma research
pub_type: 杂志文章,评审
doi:
更新日期:1997-08-01 00:00:00
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pub_type: 杂志文章
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journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200102000-00001
更新日期:2001-02-01 00:00:00
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journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e328344958c
更新日期:2011-04-01 00:00:00
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journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000174
更新日期:2015-10-01 00:00:00
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journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199706000-00005
更新日期:1997-06-01 00:00:00
abstract::In patients with metastatic cancer, gut microbiome composition differs between responder and non-responders to immune checkpoint inhibitors. However, there is little consensus on the microbiome taxa associated with response or lack of response. Additionally, recognized confounders of gut microbiome composition have ge...
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pub_type: 杂志文章
doi:10.1097/CMR.0000000000000656
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doi:10.1097/00008390-200108000-00012
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journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000195
更新日期:2015-12-01 00:00:00
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journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000245
更新日期:2016-08-01 00:00:00