Defect of insulin receptor in insulin-resistant variants of Cloudman S91 mouse melanoma cells.

Abstract:

:To study the mechanisms by which insulin regulates proliferation, we have compared wild-type Cloudman melanoma cells, whose growth in inhibited by insulin (insinh) to variant lines that were genetically selected for resistance to insulin (insres). Scatchard analysis of insulin binding to five insres) lines and six insres variants revealed a marked reduction in the number of high-affinity binding sites for insulin in the insres lines, and insres lines displayed an abnormal beta-subunit of the insulin receptor. During autophosphorylation of the wheat germ agglutinin-purified receptor, the beta-subunit apparently underwent proteolytic degradation. This proteolysis was ATP-dependent and was prevented by bovine pancreatic trypsin inhibitor, and phenylmethylsulphonyl fluoride, but not by aprotinin or leupeptin. Receptor proteolysis was not observed in wild-type lines. The results suggest that insulin resistance in the mutant Cloudman melanoma cells is apparently due to proteolysis of the beta-subunit of insulin receptor which, in turn, alters insulin binding capacity of the cells and blocks their anti-proliferative response to the hormone.

journal_name

Melanoma Res

journal_title

Melanoma research

authors

Slominski A,McNeely T,Pawelek J

doi

10.1097/00008390-199207000-00006

subject

Has Abstract

pub_date

1992-07-01 00:00:00

pages

115-22

issue

2

eissn

0960-8931

issn

1473-5636

journal_volume

2

pub_type

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