Abstract:
:This study examines whether 6-thioguanine resistant T cells (mutant) from metastatic melanoma patients are enriched for melanoma-associated T cells compared to T cells obtained analogously without thioguanine selection (wild-type). Melanoma-associated antigen pentamer staining was performed on 5 tumour and 9 peripheral blood samples from metastatic melanoma patients. T cell receptor beta chain repertoire was examined via Sanger sequencing of mutant and wild-type in blood and tumour from metastatic melanoma patients at times of tumour progression (n = 8) and via Illumina sequencing in tumour derived T cells and in uncultured T cells (uncultured), wild-type and mutant from blood before and after immune checkpoint blockade (n = 1). Mutant from tumour (3 of 5; P < 0.001), but not blood (0 of 9), were enriched compared to wild-type for binding melanoma-associated antigen pentamers. T cell receptor beta analysis in patients with tumour progression (n = 8) detected increased melanoma associated T cells in mutant compared to wild-type from blood (Monte Carlo P = 10). Comparison of blood samples before and after immune checkpoint blockade with prior tumor from one metastatic melanoma patient detected increased T cell receptor beta sharing between tumour and mutant compared to tumour and wild-type or tumour and uncultured: 11.0% (72/656), 1.5% (206/13 639) and 1.3% (381/29 807), respectively (Monte Carlo P = 10 for mutant versus wild-type and mutant versus uncultured). These data demonstrate that mutant in metastatic melanoma patients are enriched for melanoma-associated T cells and are candidate probes to study in vivo melanoma-reactive T cells.
journal_name
Melanoma Resjournal_title
Melanoma researchauthors
Zuleger CL,Newton MA,Ma X,Ong IM,Pei Q,Albertini MRdoi
10.1097/CMR.0000000000000625subject
Has Abstractpub_date
2020-02-01 00:00:00pages
52-61issue
1eissn
0960-8931issn
1473-5636pii
00008390-202002000-00005journal_volume
30pub_type
杂志文章abstract::Many melanoma epitopes are presented to cytotoxic T-lymphocytes (CTLs) by major histocompatibility complex (MHC) class I molecules, and it is reasonable to expect that the epitopes would be good substrates for the transporter associated with antigen processing (TAP), as TAP plays a major role in the transport of pepti...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199808000-00008
更新日期:1998-08-01 00:00:00
abstract::The aim of our study was to evaluate the clinical and morphological features of primary malignant melanomas of the urinary bladder. We obtained information on five such cases from three different institutions. These were three men and two women between 52 and 76 years of age. Three tumors presented with hematuria, one...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000300
更新日期:2016-12-01 00:00:00
abstract::Proteomics provides a powerful approach for screening alterations in protein expression and post-translational modification associated with particular human diseases. In this study, the analysis of protein expression was focused on malignant melanoma in order to determine the candidate genes involved in tumour progres...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200508000-00002
更新日期:2005-08-01 00:00:00
abstract::Conjunctival melanoma is a rare disease, and little is known about its molecular background. Here, we present the case of a 48-year-old patient with conjunctival melanoma and metachronic lymph node and skin metastasis with KRAS p.K117Y mutation in exon 4 in all the lesions. The cancer genome interpreter predicted this...
journal_title:Melanoma research
pub_type: 杂志文章,评审
doi:10.1097/CMR.0000000000000423
更新日期:2018-04-01 00:00:00
abstract::Our previous in vivo studies indicated that a phenolic thioether amine (PTEA), 4-S-cysteaminylphenol (CAP), selectively disintegrates melanocytes of black hair and skin, and inhibits the growth of murine and human malignant melanomas. To elucidate the mechanism of the in vivo melanocytotoxicity and anti-melanoma effec...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199211000-00002
更新日期:1992-11-01 00:00:00
abstract::Ipilimumab is approved for adjuvant melanoma treatment at a dose of 10 mg/kg, but its use is limited owing to high toxicity and treatment-associated costs. We retrospectively analyzed 29 patients who underwent complete resection of stage IIC-III melanoma and were treated with ipilimumab 3 mg/kg in an adjuvant setting....
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000593
更新日期:2019-12-01 00:00:00
abstract::Melanoma is one of the most common cancers worldwide and its incidence has been increasing over the past few decades. Gallic acid (GA) can inhibit the growth of human cancer cells in vitro and in vivo. However, there is no available information to address the effects of GA on migration and invasion of human skin cance...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e3283414444
更新日期:2011-08-01 00:00:00
abstract::We have observed that an early increase in the CD4+/CD8+ ratio of metastatic melanoma patients during chemoimmunotherapy is the most favourable independent prognostic factor. In this study, 87 patients with metastatic melanoma were monitored for peripheral blood lymphocyte subsets (CD4+ and CD8+) before and during che...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200412000-00009
更新日期:2004-12-01 00:00:00
abstract::The cholesterol-lowering medications, statins, inhibit cellular proliferation and induce apoptosis in an array of cancer cell lines, including melanoma. We investigated the apoptotic mechanism of lovastatin on human melanoma cell lines in vitro. The cytotoxicity of statins on multiple cell lines was examined by Cell T...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200504000-00001
更新日期:2005-04-01 00:00:00
abstract::Cerebral metastases from melanoma are correlated with a poor prognosis. Temozolomide is an oral alkylating agent that can cross the blood-brain barrier and in phase II and III trials, patients with advanced metastatic melanoma achieved overall response rates of 13 to 21%. The present study evaluated the efficacy and t...
journal_title:Melanoma research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/01.cmr.0000136707.60108.ab
更新日期:2004-08-01 00:00:00
abstract::Primary malignant melanoma of the esophagus is an extremely rare, but highly aggressive, tumor. The prognosis for primary malignant melanoma of the esophagus remains dismal in most literatures. We report here a case of primary malignant melanoma of the esophagus that was treated by surgical resection and, additionally...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e3283307c8a
更新日期:2010-02-01 00:00:00
abstract::Melanogenesis appears to be a unique target to develop anti-tumour agents specific for malignant melanoma. Among the anti-melanoma compounds that we have examined, 4-S-cysteaminylphenol (4-S-CAP), a phenolic amine, was found to have the most promising anti-melanoma effects. To further improve the efficacy as anti-mela...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200404000-00006
更新日期:2004-04-01 00:00:00
abstract::Reversible oxidation sensitivity of N-Oct-3 DNA binding activity was seen when melanoma extracts and recombinant Brn-2 protein were treated with a variety of metals, hydrogen peroxide and the cysteine disulphide bond forming agent diamide. Western blot analysis of diamide-oxidized N-Oct-3 protein indicated that this w...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199802000-00002
更新日期:1998-02-01 00:00:00
abstract::Anecdotal cases of partial or complete responses have been reported after ipilimumab therapy for stage IV melanoma with brain metastasis treated earlier by surgery or radiosurgery. We report the first case of ipilimumab monotherapy resulting in durable complete remission of untreated, progressive brain metastases in a...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e3283364a37
更新日期:2010-06-01 00:00:00
abstract::Large population-based studies have shown a significant association between melanoma and lymphoid neoplasia, particularly non-Hodgkin's lymphoma (NHL) and chronic lymphocytic leukaemia (CLL), that is independent of any treatment received for the initial tumour. This study examines the presentation, diagnosis, treatmen...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200110000-00012
更新日期:2001-10-01 00:00:00
abstract::Between 1983 and 1992, 21 patients with extremity stage IIIA or IIIAB melanoma underwent hyperthermic isolation limb perfusion (HILP) with cisplatin in dosages varying from 26 to 237 mg/m2 as part of a pharmacokinetics and maximum-tolerated dose study. Extremity temperatures were up to 40 degrees C and the pH was cont...
journal_title:Melanoma research
pub_type: 临床试验,杂志文章
doi:
更新日期:1994-03-01 00:00:00
abstract::The aim of the study was to identify the frequency of asymptomatic brain metastases detected by computed tomography (CT) scans in patients with metastatic cutaneous melanoma referred to first-line systemic treatment. Between 1995 and 2009, 697 Danish patients were screened with a contrast-enhanced CT scan of the brain...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e32835ae915
更新日期:2013-02-01 00:00:00
abstract::To investigate the diagnostic value of ultrasound-guided core needle biopsy (US-CNB) in suspected cases of lymph node metastasis from cutaneous melanoma. All patients with cutaneous melanoma followed in Saint-Louis Hospital between 2006 and 2010 who underwent US-CNB for suspicion of melanoma lymph node metastasis were...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000161
更新日期:2015-12-01 00:00:00
abstract::Preoperative assessment of melanoma invasion by sonometry has been reported to exhibit a high correlation with postoperative histometry based on the determination of a regression coefficient. We believe that this approach is not adequate and that it can be misleading. We therefore applied an appropriate statistical ap...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199610000-00007
更新日期:1996-10-01 00:00:00
abstract::Before licensing, ipilimumab was first made available to previously treated advanced melanoma patients through an expanded access programme (EAP) across Europe. We interrogated data from UK EAP patients to inform future clinical practice. Clinicians registered in the UK EAP provided anonymized patient data using a pre...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000185
更新日期:2015-10-01 00:00:00
abstract::The mechanism of action of fotemustine, a relatively new chloroethylnitrosourea, was evaluated in human melanoma cells in order to assess its potential as an agent for hyperthermic limb perfusion. Fotemustine was more toxic to O6-alkylguanine methyl transferase (AGT) deficient (Mer-) cells than Mer+ cells, implicating...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199802000-00012
更新日期:1998-02-01 00:00:00
abstract::Dermoscopy is a non-invasive technique that can be utilized for the clinical diagnosis of pigmented lesions. The aim of this study was to assess the utilization and beliefs about the usefulness of dermoscopy in the evaluation of pigmented lesions by physicians in dermatology residency programmes, and to determine the ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200212000-00010
更新日期:2002-12-01 00:00:00
abstract::We studied the levels of serum copper and zinc as possible diagnostic factors or markers for the early detection of patients with melanoma. Levels were determined in 35 melanoma patients at various clinical stages and in 39 healthy persons. Measurements were performed by atomic absorption spectroscopy using 5100-PC-Pe...
journal_title:Melanoma research
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract::Single-agent anti-PD1 antibodies are usually very well tolerated, but serious toxicity can still occur. Despite the PD-1 pathway seems to be relevant in the pathogenesis of immune-related myositis, anti-PD1-related myositis is generally a rare side effect of the treatment and usually not serious. However, its frequenc...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000706
更新日期:2021-02-01 00:00:00
abstract::Little is known on whether melanocortin 1 receptor (MC1R) associated cutaneous melanoma (CM) risk varies depending on histological subtype and body site, and whether tumour thickness at diagnosis (the most important prognostic factor for CM patients) differs between MC1R variant carriers and wild-type individuals. We ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000668
更新日期:2020-10-01 00:00:00
abstract::Cell free extracts from metastases of human melanoma contain a highly active ribonucleoside diphosphate reductase (RR) which uses guanosine diphosphate (GDP) as substrate and deoxythymidine triphosphate (dTTP) as effector. No activity could be detected in these extracts when cytidine diphosphate (CDP) was used as the ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199212000-00014
更新日期:1992-12-01 00:00:00
abstract:BACKGROUND:Melanoma is the leading cause of death from skin cancers and its etiology is complex. Recent discoveries related to genetic risk factors are helping us to understand melanoma pathogenesis better. Nuclear factor-κB (NF-κB) has a critical role in immunity, inflammation, and tumor growth. The 94ins/del ATTG (rs...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000267
更新日期:2016-08-01 00:00:00
abstract::The goal of this study was to determine the effect of oral melatonin in divided doses on plasma melatonin levels in patients with metastatic melanoma. Hourly blood samples were obtained from five patients for 24 h prior to melatonin administration and for 24 h during oral administration of melatonin, 50 mg every 4 h. ...
journal_title:Melanoma research
pub_type: 临床试验,杂志文章
doi:10.1097/00008390-199402000-00009
更新日期:1994-02-01 00:00:00
abstract::The aim of this study was to evaluate metastatic latency and survival after the occurrence of metastases in patients with choroidal/ciliary body melanoma treated with proton therapy. This was a retrospective cohort study. All consecutive patients with choroidal/ciliary body melanoma treated with proton therapy between...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000519
更新日期:2020-06-01 00:00:00
abstract::Ninety primary melanoma patients were studied to investigate the importance of adopting the simultaneous use of patent blue dye (PBD) and lymphoscintigraphy plus gamma detection probe to locate the sentinel node (SN). In total 135 SNs in 105 basins were visualized preoperatively under a gamma camera after lymphoscinti...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199912000-00008
更新日期:1999-12-01 00:00:00