Abstract:
:Our previous in vivo studies indicated that a phenolic thioether amine (PTEA), 4-S-cysteaminylphenol (CAP), selectively disintegrates melanocytes of black hair and skin, and inhibits the growth of murine and human malignant melanomas. To elucidate the mechanism of the in vivo melanocytotoxicity and anti-melanoma effect, this study examined the selectivity and specificity of PTEA incorporation into malignant melanoma cells using [14C]4(2)-S-CAP, and then identified a PTEA-binding protein through a ligand binding assay using [125I]-labelled cell lysates. Whole body autoradiography showed that [14C]4-S-CAP is selectively incorporated and accumulated into the eye and tumours of a B16 melanoma-bearing mouse. SK MEL 23 human melanoma cells also showed a steady accumulation of [14C]4-S-CAP (threefold at least up to 5 min) and of [14C]2-S-CAP (sevenfold up to 20 min), compared with that of HeLa cells and fibroblasts, which plateau at 5 min. Chromatography of 4-S-CAP on an affinity column (both CH- and CNBr-activated Sepharose 4B) identified a 58 kD protein in melanoma cells, which was present at very low levels in HeLa cells; this 58 kD protein was retained by both 4-S- and 2-S-CAP affinity columns, but not by columns of a phenolic thioether (cysteinylphenol: CP) or a phenolic thioether amide (N-acetyl-4-S-CAP), and could be retrieved by either 4-S or 2-S-CAP but not by CP and N-acetyl-4-S-CAP. This protein was glycosylated, and contained mannose residues.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Melanoma Resjournal_title
Melanoma researchauthors
Yamada K,Jimbow Kdoi
10.1097/00008390-199211000-00002subject
Has Abstractpub_date
1992-11-01 00:00:00pages
225-33issue
4eissn
0960-8931issn
1473-5636journal_volume
2pub_type
杂志文章abstract::This randomized phase II trial was performed to define the activity and toxicity of the combination of dacarbazine (DTIC), carmustine (BCNU), cisplatin (DDP) and tamoxifen (DBDT regimen) versus DTIC alone in patients with metastatic melanoma. Sixty patients with metastatic melanoma were randomly assigned to receive BC...
journal_title:Melanoma research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00008390-200104000-00015
更新日期:2001-04-01 00:00:00
abstract::Conditional survival in patients with localized primary cutaneous malignant melanoma (CMM) is well described. However, conditional recurrence-free survival (RFS) has not been investigated before. The aim of this study was to determine conditional RFS and test for time dependency in prognostic factors in patients with ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000479
更新日期:2018-12-01 00:00:00
abstract::Acral lentiginous melanoma (ALM) is the most frequent type of cutaneous melanoma in the Asian and African populations, but the fourth distinct variant of cutaneous melanoma in the Caucasian population. Histological criteria and prognosis of ALM remain controversial. A retrospective study, showing epidemiological, clin...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e3282f1d2b6
更新日期:2010-06-25 00:00:00
abstract::Significant advances have been made in the treatment of melanoma by targeting key cellular pathways, but additional targets are needed as many patients do not respond or relapse with resistant disease. MicroRNA-155 (MiR-155) has previously been shown to regulate melanoma cell growth and acts as a tumor suppressor. We ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000545
更新日期:2019-04-01 00:00:00
abstract::Electrochemotherapy has been demonstrated to be an effective treatment for cutaneous cancers. The treatment includes administering a chemotherapeutic agent followed by electric pulses which are applied directly to the tumour. The pulses facilitate delivery of drug through the plasma membrane. Enhanced delivery is rest...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199612000-00004
更新日期:1996-12-01 00:00:00
abstract::The demonstration that zinc-finger transcriptional repressors can control E-cadherin expression in epithelial cells has provided a new avenue of research in the field of epithelial-mesenchymal transition (EMT). One of these zinc-finger molecules is the transcription factor Snail, which controls gastrulation and neural...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200508000-00012
更新日期:2005-08-01 00:00:00
abstract::Melanoma is the deadliest form of skin cancer. Ipilimumab, a novel immunotherapy, is the first treatment shown to improve survival in patients with metastatic melanoma in large randomized controlled studies. The most concerning side effects reported in clinical studies of ipilimumab fall into the category of immune-re...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e32835c7e68
更新日期:2013-02-01 00:00:00
abstract::Inositol hexaphosphate (IP6) also called phytic acid is a polyphosphorylated carbohydrate naturally found in cereals, nuts, grains, and high-fiber-containing foods. It has been shown to inhibit the growth of many different tumor cell lines both in vitro and in vivo like colon, pancreas, liver, prostate, and even melan...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000577
更新日期:2019-06-01 00:00:00
abstract::CD8 T-cell infiltration of metastatic melanoma may be a useful biomarker for prediction of prognosis and response to therapy. The heterogeneous distribution of CD8 T cells within a single tumor, and across different tumors within a single patient, may complicate quantification of infiltration. However, the impact of h...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000330
更新日期:2017-06-01 00:00:00
abstract::Lymphoscintigraphy (LS) has been performed for 8 years in patients of the Sydney Melanoma Unit, to define lymphatic drainage patterns. Over the past 2 years, LS has also been used to locate the sentinel lymph node prior to surgery. Our technique for LS and subsequent sentinel node biopsy has an accuracy of 97%. All se...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199412000-00009
更新日期:1994-12-01 00:00:00
abstract::Prognostic groups defined by lactate dehydrogenase concentration and number of organ sites containing metastases have been reported for patients treated with dabrafenib and trametinib for advanced melanoma. We aimed to validate these prognostic groups for patients treated with vemurafenib and cobimetinib in the coBRIM...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000634
更新日期:2020-06-01 00:00:00
abstract::Melanoma cells are unusual because, unlike most epithelial tumours, constitutive expression of HLA class II antigens is common. We have previously demonstrated that a peptide-specific CD4+ T-cell clone proliferates briskly in response to peptide and HLA class II expressing melanoma cell lines derived from metastases. ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199904000-00010
更新日期:1999-04-01 00:00:00
abstract::To study the mechanisms by which insulin regulates proliferation, we have compared wild-type Cloudman melanoma cells, whose growth in inhibited by insulin (insinh) to variant lines that were genetically selected for resistance to insulin (insres). Scatchard analysis of insulin binding to five insres) lines and six ins...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199207000-00006
更新日期:1992-07-01 00:00:00
abstract::Many melanoma epitopes are presented to cytotoxic T-lymphocytes (CTLs) by major histocompatibility complex (MHC) class I molecules, and it is reasonable to expect that the epitopes would be good substrates for the transporter associated with antigen processing (TAP), as TAP plays a major role in the transport of pepti...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199808000-00008
更新日期:1998-08-01 00:00:00
abstract::Locally advanced cutaneous melanoma has marked quality-of-life implications; however, the patient experience of symptom management and subsequent impact on quality of life has not been well described. This study aims to address the impact on patients of advanced cutaneous melanoma through qualitative interviews. Adult...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000425
更新日期:2018-04-01 00:00:00
abstract::Immunosuppressed patients are at increased risk of skin cancer. A 67-year-old renal transplant recipient developed a nodular malignant melanoma after 30 years of immunosuppression with azathioprine and prednisolone. The patient died of metastatic disease 3 months after the diagnosis was made. The function of the renal...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e32831bc44a
更新日期:2009-08-01 00:00:00
abstract::We studied the response of mouse B16F10 and SK-MEL-28 and SK-MEL-1 human melanoma cell lines to treatment with 4-hydroxyanisole (4-HA), and attempted to relate the response to the dopa oxidase levels and the morphological characteristics of each cell line. Clear dose-response curves were observed after 24 h of treatme...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199710000-00003
更新日期:1997-10-01 00:00:00
abstract::Melanoma tumors have been shown to comprise both invasive and proliferative cell subpopulations. These populations are highly plastic, thus hampering full characterization and therapeutic targeting of dormant and partially dedifferentiated invasive cells. We have reported, previously, that melanoma cells grown in a se...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e328351e7c4
更新日期:2012-06-01 00:00:00
abstract::In this study we evaluated the overexpression status of HER-2 and its prognostic significance on survival in patients with thick cutaneous malignant melanoma. The immuno-alkaline phosphatase antigen detection technique was applied to archival diagnostic material from 51 patients with primary lesions measuring >or= 10 ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200204000-00006
更新日期:2002-04-01 00:00:00
abstract::Ipilimumab (anti-CTLA-4 antibody) is a new tool for the treatment of metastatic melanoma patients that has led to an improvement in survival rates worldwide. New types of toxicities have been described with ipilimumab called 'immune-related adverse events' or irAEs. Here, we report an acute and steroid resistant case ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e32835fb524
更新日期:2013-06-01 00:00:00
abstract::To examine the distribution of occult micrometastases that could be potential sources of recurrence, complete maps of microscopic and submicroscopic metastases in entire inguinal lymph node basins were generated in 13 melanoma patients who had undergone elective or therapeutic lymphadenectomy. Occult micrometastases w...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199908000-00010
更新日期:1999-08-01 00:00:00
abstract::The CD10 antigen is a neutral endopeptidase expressed by a variety of mesenchymal tumours (haemopoietic or not), including a subset of malignant melanomas. We investigated the expression of CD10 in formalin-fixed, paraffin-embedded tissue specimens of 72 cutaneous melanomas (28 primary, 26 metastatic to the skin and 1...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200206000-00007
更新日期:2002-06-01 00:00:00
abstract::Comparative genomic hybridization was used to map copy number abnormalities in 48 short-term cell cultures established from different stages and types of human melanoma. A variety of random and non-random chromosomal alterations were detected, with gains within chromosomes 20q, 7q, 7p, 20p, 6p and 17q and losses in 9p...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200310000-00007
更新日期:2003-10-01 00:00:00
abstract::Selective BRAF inhibitors (BRAFi) yield objective responses in 50% of patients with metastatic BRAF V600E mutant melanoma. Adding an MEK inhibitor increases this response rate to 70%. Limited data are available on the outcomes of unresectable stage III patients, and it remains unclear whether BRAF-targeted therapy can...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000214
更新日期:2016-02-01 00:00:00
abstract::Familial atypical multiple mole-melanoma (FAMMM) syndrome is characterized by the familial occurrence of malignant melanoma of the skin in combination with multiple atypical precursor naevi. In the present study we performed linkage analysis in seven Dutch FAMMM families to define the relationship between the ultimate...
journal_title:Melanoma research
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
abstract::Melanoma is among the most chemotherapy-resistant malignancies. Numerous new agents have been developed that target specific molecules on cancer cells, including the monoclonal antibody trastuzumab, which targets Her2/neu and has been very beneficial in the treatment of breast cancer. There are conflicting reports in ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/01.cmr.0000130874.33504.2f
更新日期:2004-06-01 00:00:00
abstract:BACKGROUND:Melanoma is the leading cause of death from skin cancers and its etiology is complex. Recent discoveries related to genetic risk factors are helping us to understand melanoma pathogenesis better. Nuclear factor-κB (NF-κB) has a critical role in immunity, inflammation, and tumor growth. The 94ins/del ATTG (rs...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000267
更新日期:2016-08-01 00:00:00
abstract::We describe a rare case of transplacental-transmitted maternal melanoma to the placenta and foetus during the second pregnancy of a 28-year-old woman. She was aware of a greyish-brown nodular lesion on the right gluteus during her first pregnancy. On histological examination, this lesion resulted to be an amelanocitic...
journal_title:Melanoma research
pub_type: 杂志文章,评审
doi:10.1097/CMR.0b013e32833faf6a
更新日期:2010-12-01 00:00:00
abstract::In a previous qualitative study it has been shown that the incorporation of pre-existing collagen bundles from the reticular dermis into the bulk of melanoma lesions metastatic to the skin indicates rapid systemic spread. In the present study the amount of pre-existing dermal collagen in the bulk of the melanoma lesio...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199804000-00010
更新日期:1998-04-01 00:00:00
abstract::The CXCR4 chemokine receptor plays an essential role in the homing of cells to organs expressing its ligand, CXCL12. CXCR4 expressed on tumor cells might regulate their traffic during metastasis. Here, we investigated whether the activation of CXCR4 on B16 murine melanoma cells regulates biological functions associate...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000346
更新日期:2017-08-01 00:00:00