Abstract:
:The CXCR4 chemokine receptor plays an essential role in the homing of cells to organs expressing its ligand, CXCL12. CXCR4 expressed on tumor cells might regulate their traffic during metastasis. Here, we investigated whether the activation of CXCR4 on B16 murine melanoma cells regulates biological functions associated with metastasis, in vitro and in vivo. Flow cytometry and PCR analysis showed that B16 constitutively expresses high levels of CXCR4 (CXCR4-B16). Biological assays showed that the activation of CXCR4, by its ligand CXCL12, increases the migration, invasion, and proliferation of CXCR4-B16. AMD3100 significantly inhibited the stimulatory migrating effect induced by CXCL12. Treatment of CXCR4-B16 with CXCL12 increases their adhesion to liver sinusoidal endothelial cell (LSEC) monolayers. LSEC, expressing CXCL12, increased the migration of CXCR4-B16. In a liver metastasis model, CXCR4-B16 metastasis was associated with an increased expression of CXCL12 in LSEC territories. CXCR4-B16 cells were located close to LSEC microenvironments expressing CXCL12. Increased liver metastasis was observed after injecting CXCR4-B16 cells previously treated with CXCL12. Our results provide evidence showing that CXCR4 plays an important role in regulating biological functions associated with B16 liver metastasis.
journal_name
Melanoma Resjournal_title
Melanoma researchauthors
Mendt M,Cardier JEdoi
10.1097/CMR.0000000000000346subject
Has Abstractpub_date
2017-08-01 00:00:00pages
300-308issue
4eissn
0960-8931issn
1473-5636journal_volume
27pub_type
杂志文章abstract::The objective of this study is to assess the expression of heat shock protein 105 (HSP105) in melanoma and benign melanocytic lesions. The expression of HSP105 in 62 human melanoma samples--46 primary and 16 metastatic lesions--and 42 melanocytic naevi samples, was assessed by immunohistochemistry. Western blotting wa...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e3282fe9a16
更新日期:2008-06-01 00:00:00
abstract::Prognostic blood biomarkers for patients with uveal melanoma have not been identified. Tumor monosomy-3 is strongly associated with the development of metastatic disease. Tumor expression of human leukocyte antigen class I molecules and insulin-like growth factor (IGF)-1 receptor has also been associated with the deve...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e32835b7154
更新日期:2013-02-01 00:00:00
abstract::The aim of this study was to evaluate S100B in bone marrow (BM) plasma from malignant melanoma patients. BM aspirates and peripheral blood (PB) plasma from 56 patients and BM aspirates from 29 healthy volunteers were collected. S100B was measured using an immune radiometric assay, which is a two-site sandwich assay ba...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e3282f623d9
更新日期:2008-04-01 00:00:00
abstract::The correlation between ultraviolet radiation of the skin and melanoma incidence in humans is well established. Interestingly, epidemiologic data suggest also a correlation to an increased BMI pointing to metabolic trigger factors in melanoma pathogenesis. To substantiate this connection, we studied the expression of ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000445
更新日期:2018-08-01 00:00:00
abstract::In a previous large randomized, open-label study, retrospective subset analysis revealed that the addition of the Bcl-2 antisense oligonucleotide oblimersen to dacarbazine (Dac) significantly improved overall survival, progression-free survival, and the response rate in chemotherapy-naive patients with advanced melano...
journal_title:Melanoma research
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1097/CMR.0000000000000056
更新日期:2014-06-01 00:00:00
abstract::Several hypotheses have explicitly implicated the role of an altered redox status of melanin in the aetiology of melanoma and macular degeneration. The balance between the intrinsic anti-oxidant and pro-oxidant properties of melanin is lost, resulting in an altered redox phenotype. We propose that such an alteration o...
journal_title:Melanoma research
pub_type: 杂志文章,评审
doi:10.1097/01.cmr.0000195699.35143.df
更新日期:2006-02-01 00:00:00
abstract::When cancer cells, including melanoma cells, are genetically altered to secrete cytokines, irradiated and injected into subjects, long-term antitumour immunity is induced. Optimally, existing melanomas induced to produce cytokines in vivo could stimulate this same immune response. Although in vivo electroporation enha...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200012000-00010
更新日期:2000-12-01 00:00:00
abstract::Treatment of patients with metastatic melanoma with either dacarbazine (DTIC) or interferon-alpha (IFNalpha) as single drugs, or in combination, results in a response rate of approximately 15-20%. This study evaluated the activity and toxicity following treatment with a combination of DTIC, IFNalpha2b and verapamil (V...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200302000-00014
更新日期:2003-02-01 00:00:00
abstract::To study the mechanisms by which insulin regulates proliferation, we have compared wild-type Cloudman melanoma cells, whose growth in inhibited by insulin (insinh) to variant lines that were genetically selected for resistance to insulin (insres). Scatchard analysis of insulin binding to five insres) lines and six ins...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199207000-00006
更新日期:1992-07-01 00:00:00
abstract::Superantigens like the Staphylococcus enterotoxin A (SEA) can direct cytotoxic T lymphocytes expressing certain T cell receptor V beta regions to lyse MHC class II-positive target cells. This superantigen-dependent cellular cytotoxicity (SDCC) has been extended to MHC class II-negative tumour cells by targeting T cell...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199706000-00005
更新日期:1997-06-01 00:00:00
abstract::The EORTC Melanoma Cooperative Group conducted a phase II trial (study number 18891) to study the effect of Toremifene in 45 patients with advanced, metastatic melanoma. Male and female patients, median age 61 (range 23-88) years received a mean total dose of 11 g (4.3-62.6) orally for at least 6 weeks. No objective r...
journal_title:Melanoma research
pub_type: 临床试验,杂志文章,多中心研究
doi:
更新日期:1993-04-01 00:00:00
abstract::This study addressed the impact of exposure to ultraviolet radiation on the risk of cutaneous malignant melanoma (CMM), as well as the behavioral components at stake in its occurrence. We performed a one-to-one unmatched case-control study among subjects aged 20 years or more with naturally non-pigmented skin in Germa...
journal_title:Melanoma research
pub_type: 杂志文章,多中心研究
doi:10.1097/00008390-199404000-00001
更新日期:1994-04-01 00:00:00
abstract::The p53 pathway regulates stress response, and variations in p53, MDM2, and MDM4 may predispose an individual to tumor development. The aim of this study was to study the impact of genetic variation on sporadic and hereditary melanoma. We have analyzed a combination of three functionally relevant variants of the p53 p...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000063
更新日期:2014-06-01 00:00:00
abstract::Inositol hexaphosphate (IP6) also called phytic acid is a polyphosphorylated carbohydrate naturally found in cereals, nuts, grains, and high-fiber-containing foods. It has been shown to inhibit the growth of many different tumor cell lines both in vitro and in vivo like colon, pancreas, liver, prostate, and even melan...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000577
更新日期:2019-06-01 00:00:00
abstract::Putative tumour suppressor genes CDKN2A and CDKN2B (on chromosome 9p21) and CDKN2A-interacting cell growth regulatory genes CDK4 and Id-1 have been demonstrated to be involved in the pathogenesis of malignant melanoma (MM). Mutation analysis of these candidate genes was performed in MM families from southern Italy wit...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200312000-00006
更新日期:2003-12-01 00:00:00
abstract::The presence or absence of melanoma cells in human peripheral blood has recently been shown to be associated with disease prognosis, including overall survival. The detection of tyrosinase mRNA-positive circulating melanoma cells by reverse transcription-polymerase chain reaction (RT-PCR) has been limited to dissemina...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200008000-00007
更新日期:2000-08-01 00:00:00
abstract::The demonstration that zinc-finger transcriptional repressors can control E-cadherin expression in epithelial cells has provided a new avenue of research in the field of epithelial-mesenchymal transition (EMT). One of these zinc-finger molecules is the transcription factor Snail, which controls gastrulation and neural...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200508000-00012
更新日期:2005-08-01 00:00:00
abstract::We studied the efficacy, tolerability and clinical courses of dabrafenib in patients with metastatic melanoma who were ineligible for enrolment into a clinical trial. Between July 2011 and May 2013, patients with unresectable stage III or stage IV, V600-mutated metastatic melanoma who were not eligible for inclusion i...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000036
更新日期:2014-04-01 00:00:00
abstract::To date, no study of melanoma patients who have undergone delayed lymph node dissection (DLND) has focused on the independent prognostic factors of overall survival, as calculated from surgery on the primary. Using Kaplan-Meier estimates and Cox's proportional hazard model, the significance of prognostic factors was e...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200010000-00011
更新日期:2000-10-01 00:00:00
abstract::There is a scarcity of available data on unmet information needs (UINs) of melanoma patients (MPs) from Germany and of MPs with clinical stage IV. In a multicenter cross-sectional survey, we explored the UINs of 529 MPs by applying a standardized questionnaire. Subgroup differences in scope and contents of UINs were d...
journal_title:Melanoma research
pub_type: 杂志文章,多中心研究
doi:10.1097/CMR.0000000000000464
更新日期:2019-04-01 00:00:00
abstract::High-dose cisplatin regimens have been shown to be highly active in advanced melanoma patients but are associated with unacceptable side effects. In order to increase the platinum dose but avoid severe side effects, we treated 15 dacarbazine (DTIC)-resistant metastatic melanoma patients with a combination regimen of c...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200108000-00012
更新日期:2001-08-01 00:00:00
abstract::Radiotherapy after lymph node dissection is recommended in high-risk melanoma cases. The aim of this study is to assess whether intensity-modulated radiotherapy (IMRT) offers advantages over three-dimensional conformal radiotherapy (3DCRT) in the groin nodal basin. Fifteen consecutively treated patients (5 3DCRT and 1...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000304
更新日期:2017-02-01 00:00:00
abstract::Ninety primary melanoma patients were studied to investigate the importance of adopting the simultaneous use of patent blue dye (PBD) and lymphoscintigraphy plus gamma detection probe to locate the sentinel node (SN). In total 135 SNs in 105 basins were visualized preoperatively under a gamma camera after lymphoscinti...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199912000-00008
更新日期:1999-12-01 00:00:00
abstract::Seasonal variation in melanoma diagnoses has been observed in numerous studies that used calendar time indicators. Depending on the latitude (and altitude) of the study region, the magnitude of seasonal and year-to-year variation of ultraviolet radiation (UVR) is neglected in these studies. An alternative approach com...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000205
更新日期:2015-12-01 00:00:00
abstract::The universal second messenger cAMP regulates numerous cellular processes. Although the cAMP-signaling pathway leads to induction of gene transcription, it remains unknown whether this pathway contributes toward suppression of transcription. Here, we show that blockade of cAMP signaling using MDL12330A led to an incre...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000363
更新日期:2017-10-01 00:00:00
abstract::Cellular senescence is a major barricade on the path of cancer development, yet proteins secreted from senescent cells exert complex and often discordant effects on subsequent cancer evolution. Somatic genome alternations driving the formation of nevi and melanoma are efficient inducers of cellular senescence. Melanoc...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000671
更新日期:2020-08-01 00:00:00
abstract::Treatment strategies for metastatic melanoma have changed markedly in recent times due to the advent of targeted systemic therapies. In addition to these developments, surgery remains a useful adjunct that can confer survival benefits in selected patients. In this review, we examine the current literature to highlight...
journal_title:Melanoma research
pub_type: 杂志文章,评审
doi:10.1097/CMR.0000000000000128
更新日期:2015-02-01 00:00:00
abstract::Melanoma cells are unusual because, unlike most epithelial tumours, constitutive expression of HLA class II antigens is common. We have previously demonstrated that a peptide-specific CD4+ T-cell clone proliferates briskly in response to peptide and HLA class II expressing melanoma cell lines derived from metastases. ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199904000-00010
更新日期:1999-04-01 00:00:00
abstract::Immune checkpoint inhibitors targeting the programmed cell death (PD)-1 receptor have dramatically changed the landscape of metastatic melanoma treatment. Nevertheless, these immuno-modulatory agents have associated side effects, including dermatologic manifestations. To this end, we report a patient with metastatic m...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000477
更新日期:2018-10-01 00:00:00
abstract::Patients with melanoma may experience a variety of different vision symptoms, in part associated with melanoma-associated retinopathy. For several melanoma patients with or without melanoma-associated retinopathy, colour vision deficiencies, especially involving the tritan system, have been reported. The frequency of ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/01.cmr.0000222599.35062.84
更新日期:2006-10-01 00:00:00