Abstract:
:Ninety primary melanoma patients were studied to investigate the importance of adopting the simultaneous use of patent blue dye (PBD) and lymphoscintigraphy plus gamma detection probe to locate the sentinel node (SN). In total 135 SNs in 105 basins were visualized preoperatively under a gamma camera after lymphoscintigraphy. When a SN was identified intraoperatively, its radioactivity level and colour were verified and documented. Two of the SNs seen on lymphoscintigraphy were not found. Using PBD 78.52% of the SNs were identified; 95.5% were identified using the gamma detection probe. Using both methods together 98.5% of the SNs were detected. Twenty-two patients (24.4%) had pathologically positive SNs. The surgical learning curve was assessed for the two techniques. The learning curve associated with the methodology was important in finding the SN when using PBD associated with lymphoscintigraphy, but not when the gamma detection probe was used; we found a statistically significant reduction in the percentage of stained SNs found using PBD in the initial 14 SNs biopsied compared with the subsequent 121 nodes. This is important as not all institutions have access to a gamma probe. The time required to identify each SN was documented and analysed. The duration of the procedure was significantly shorter for stained SNs than for non-stained SNs, which support the use of both PBD and the gamma probe. In conclusion, SN biopsy should be performed by surgeons and nuclear medicine doctors in co-operation, both methods being adopted simultaneously to reduce the percentage of procedure failures.
journal_name
Melanoma Resjournal_title
Melanoma researchauthors
Testori A,Bartolomei M,Grana C,Mezzetti M,Chinol M,Mazzarol G,Lazzari I,Paganelli G,Geraghty JG,Andreoni B,Veronesi Udoi
10.1097/00008390-199912000-00008subject
Has Abstractpub_date
1999-12-01 00:00:00pages
587-93issue
6eissn
0960-8931issn
1473-5636journal_volume
9pub_type
杂志文章abstract::The primary treatment of a melanoma is surgical excision. An excisional biopsy is preferred, and safety margins of 1 cm for tumor thickness up to 2 mm and 2 cm for higher tumor thickness should be applied either at primary excision or in a two-step procedure. When dealing with facial, acral or anogenital melanomas, mi...
journal_title:Melanoma research
pub_type: 共识发展会议,杂志文章,实务指引
doi:10.1097/CMR.0b013e3282f0c893
更新日期:2008-02-01 00:00:00
abstract::Melanoma is among the most chemotherapy-resistant malignancies. Numerous new agents have been developed that target specific molecules on cancer cells, including the monoclonal antibody trastuzumab, which targets Her2/neu and has been very beneficial in the treatment of breast cancer. There are conflicting reports in ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/01.cmr.0000130874.33504.2f
更新日期:2004-06-01 00:00:00
abstract::Electroporation therapy (EPT) is a novel treatment modality that uses brief, high-intensity, pulsed electrical currents to enhance the uptake of chemotherapeutic agents, vaccines and genes into cells. This technique is potentially useful for patients with secondary and, possibly, some primary tumours. Nineteen patient...
journal_title:Melanoma research
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1097/00008390-200502000-00008
更新日期:2005-02-01 00:00:00
abstract::The goal of this study was to determine the effect of oral melatonin in divided doses on plasma melatonin levels in patients with metastatic melanoma. Hourly blood samples were obtained from five patients for 24 h prior to melatonin administration and for 24 h during oral administration of melatonin, 50 mg every 4 h. ...
journal_title:Melanoma research
pub_type: 临床试验,杂志文章
doi:10.1097/00008390-199402000-00009
更新日期:1994-02-01 00:00:00
abstract::Proteomics provides a powerful approach for screening alterations in protein expression and post-translational modification associated with particular human diseases. In this study, the analysis of protein expression was focused on malignant melanoma in order to determine the candidate genes involved in tumour progres...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200508000-00002
更新日期:2005-08-01 00:00:00
abstract::There is no general consensus regarding the optimal follow-up strategy for patients with melanoma. We sought to determine the utility and cost effectiveness of radiological restaging of patients with stage IIB-IIIC melanoma at the 3-year follow-up time point. A retrospective review of 210 patients diagnosed with stage...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e3283471086
更新日期:2011-08-01 00:00:00
abstract::Loss of expression of E-cadherin, the major cell-cell adhesion receptor on keratinocytes, has been linked to tumour progression in various carcinomas. As E-cadherin has been reported to be expressed in cultured human melanocytes, we questioned whether loss of E-cadherin expression may also be related to melanocytic tu...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199604000-00007
更新日期:1996-04-01 00:00:00
abstract::There is a scarcity of available data on unmet information needs (UINs) of melanoma patients (MPs) from Germany and of MPs with clinical stage IV. In a multicenter cross-sectional survey, we explored the UINs of 529 MPs by applying a standardized questionnaire. Subgroup differences in scope and contents of UINs were d...
journal_title:Melanoma research
pub_type: 杂志文章,多中心研究
doi:10.1097/CMR.0000000000000464
更新日期:2019-04-01 00:00:00
abstract::Cellular senescence is a major barricade on the path of cancer development, yet proteins secreted from senescent cells exert complex and often discordant effects on subsequent cancer evolution. Somatic genome alternations driving the formation of nevi and melanoma are efficient inducers of cellular senescence. Melanoc...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000671
更新日期:2020-08-01 00:00:00
abstract::Cerebral metastases from melanoma are correlated with a poor prognosis. Temozolomide is an oral alkylating agent that can cross the blood-brain barrier and in phase II and III trials, patients with advanced metastatic melanoma achieved overall response rates of 13 to 21%. The present study evaluated the efficacy and t...
journal_title:Melanoma research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/01.cmr.0000136707.60108.ab
更新日期:2004-08-01 00:00:00
abstract::The mechanism of selective incorporation of thiourea into melanotic melanoma was investigated by model experiments in which the effect of the compound was examined at various stages of melanogenesis in vitro. Up to 50% inhibition of dopachrome formation was observed in the tyrosinase-dopa reaction in the presence of t...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199712000-00006
更新日期:1997-12-01 00:00:00
abstract::The CD10 antigen is a neutral endopeptidase expressed by a variety of mesenchymal tumours (haemopoietic or not), including a subset of malignant melanomas. We investigated the expression of CD10 in formalin-fixed, paraffin-embedded tissue specimens of 72 cutaneous melanomas (28 primary, 26 metastatic to the skin and 1...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200206000-00007
更新日期:2002-06-01 00:00:00
abstract::Between 1983 and 1990, 59 patients with malignant melanoma were retrospectively reviewed to assess the safety, efficacy and the maximal tolerated dose of cisplatin used in hyperthermic isolated limb perfusion. The median follow-up was 29 months (range 3-54 months). The local recurrence rate was 12% in Stage I, 33% in ...
journal_title:Melanoma research
pub_type: 临床试验,杂志文章
doi:10.1097/00008390-199104000-00007
更新日期:1991-04-01 00:00:00
abstract::Metastasis is a complicated multi-step process involving interactions between tumour cells, the extracellular matrix and the vessel walls. Experimental observations suggest that leucocyte migration and function could be a suitable model in order to understand tumour cell dissemination. In the present report we show an...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199810000-00001
更新日期:1998-10-01 00:00:00
abstract::Immunosuppressed patients are at increased risk of skin cancer. A 67-year-old renal transplant recipient developed a nodular malignant melanoma after 30 years of immunosuppression with azathioprine and prednisolone. The patient died of metastatic disease 3 months after the diagnosis was made. The function of the renal...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e32831bc44a
更新日期:2009-08-01 00:00:00
abstract::Glutathione (GSH) is the major non-protein thiol in cells that plays a critical role against damage from electrophilic agents such as alkylating drugs. Selective therapeutic GSH elevation in normal but not in tumour cells has been suggested as a means of protecting host tissues against more intense doses of chemothera...
journal_title:Melanoma research
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract::Datasets from large health maintenance organizations (HMOs), particularly those with established cancer registries that report to the Surveillance, Epidemiology, and End Results program, are potentially excellent resources for studying melanoma epidemiology and outcomes. However, generalizability of the findings beyon...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000087
更新日期:2014-08-01 00:00:00
abstract::Between 6 and 14% of malignant melanomas have been reported to occur in a familial pattern. In this study 785 melanoma patients from the Victorian Melanoma Service and the private practice of a dermatologist were assessed for the total number of melanocytic naevi, the number of dysplastic naevi and other clinical char...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199810000-00011
更新日期:1998-10-01 00:00:00
abstract::Melan-A is widely used in the diagnostics of human melanoma. The immunogenicity of this glycoprotein makes it a potential target in immunotherapy and several authors have suggested its potential as a prognostic factor. Up to now there has been no clear direct evidence of changes of Melan-A expression during the progre...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0b013e328303beac
更新日期:2008-08-01 00:00:00
abstract::Reversible oxidation sensitivity of N-Oct-3 DNA binding activity was seen when melanoma extracts and recombinant Brn-2 protein were treated with a variety of metals, hydrogen peroxide and the cysteine disulphide bond forming agent diamide. Western blot analysis of diamide-oxidized N-Oct-3 protein indicated that this w...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199802000-00002
更新日期:1998-02-01 00:00:00
abstract::Inositol hexaphosphate (IP6) also called phytic acid is a polyphosphorylated carbohydrate naturally found in cereals, nuts, grains, and high-fiber-containing foods. It has been shown to inhibit the growth of many different tumor cell lines both in vitro and in vivo like colon, pancreas, liver, prostate, and even melan...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000577
更新日期:2019-06-01 00:00:00
abstract::Many melanoma epitopes are presented to cytotoxic T-lymphocytes (CTLs) by major histocompatibility complex (MHC) class I molecules, and it is reasonable to expect that the epitopes would be good substrates for the transporter associated with antigen processing (TAP), as TAP plays a major role in the transport of pepti...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199808000-00008
更新日期:1998-08-01 00:00:00
abstract::The universal second messenger cAMP regulates numerous cellular processes. Although the cAMP-signaling pathway leads to induction of gene transcription, it remains unknown whether this pathway contributes toward suppression of transcription. Here, we show that blockade of cAMP signaling using MDL12330A led to an incre...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000363
更新日期:2017-10-01 00:00:00
abstract::In a previous qualitative study it has been shown that the incorporation of pre-existing collagen bundles from the reticular dermis into the bulk of melanoma lesions metastatic to the skin indicates rapid systemic spread. In the present study the amount of pre-existing dermal collagen in the bulk of the melanoma lesio...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199804000-00010
更新日期:1998-04-01 00:00:00
abstract::The aim of this study was to evaluate metastatic latency and survival after the occurrence of metastases in patients with choroidal/ciliary body melanoma treated with proton therapy. This was a retrospective cohort study. All consecutive patients with choroidal/ciliary body melanoma treated with proton therapy between...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/CMR.0000000000000519
更新日期:2020-06-01 00:00:00
abstract::In this study we evaluated the overexpression status of HER-2 and its prognostic significance on survival in patients with thick cutaneous malignant melanoma. The immuno-alkaline phosphatase antigen detection technique was applied to archival diagnostic material from 51 patients with primary lesions measuring >or= 10 ...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200204000-00006
更新日期:2002-04-01 00:00:00
abstract::In human melanoma no complete information about the expression of the apoptosis-promoting and apoptosis-inhibiting members of the Bcl-2 family has been available to date. In this study we have investigated by Western blotting the expression pattern of Bcl-2 and its homologues Bax, Bak, Bcl-xL, Bcl-xS, Mcl-1 and Bad in...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-199806000-00001
更新日期:1998-06-01 00:00:00
abstract::The aim of this study was to determine the impact of complete response (CR) to systemic therapy on survival. We reviewed the cases of 647 chemo-naive patients with metastatic melanoma who were treated with cisplatin-vinblastine-dacarbazine or cisplatin-taxol-dacarbazine alone, or cisplatin-vinblastine-dacarbazine toge...
journal_title:Melanoma research
pub_type: 杂志文章,评审
doi:10.1097/CMR.0b013e328341445f
更新日期:2011-02-01 00:00:00
abstract::During the process of melanogenesis free radicals are generated. The aim of this study was to investigate the effects of melanogenesis in B16 melanoma on lipid peroxidation and antioxidant capacity in selected tissues of black C57BL/6J mice. The study was conducted on 24 mice: 12 healthy controls and 12 with a transpl...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/00008390-200302000-00004
更新日期:2003-02-01 00:00:00
abstract::Resistance to various antineoplastic agents is common in the clinical management of malignant melanoma. The biological mechanisms conferring these different drug-resistant phenotypes are still unclear. To identify potential factors mediating drug resistance to melanoma cells, the mRNA expression profiles of the parent...
journal_title:Melanoma research
pub_type: 杂志文章
doi:10.1097/01.cmr.0000215037.23188.58
更新日期:2006-04-01 00:00:00